• 제목/요약/키워드: blood cell analysis

검색결과 717건 처리시간 0.036초

The Changes of P-glycoprotein Activity by Interferon-γ and Tumor Necrosis Factor-α in Primary and Immortalized Human Brain Microvascular Endothelial Cells

  • Lee, Na-Young;Rieckmann, Peter;Kang, Young-Sook
    • Biomolecules & Therapeutics
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    • 제20권3호
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    • pp.293-298
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    • 2012
  • The purpose of this study was to investigate the modification of expression and functionality of the drug transporter P-glycoprotein (P-gp) by tumor necrosis factor-alpha (TNF-${\alpha}$) and interferon-gamma (IFN-${\gamma}$) at the blood-brain barrier (BBB). We used immortalized human brain microvessel endothelial cells (iHBMEC) and primary human brain microvessel endothelial cells (pHBMEC) as in vitro BBB model. To investigate the change of p-gp expression, we carried out real time PCR analysis and Western blotting. To test the change of p-gp activity, we performed rhodamin123 (Rh123) accumulation study in the cells. In results of real time PCR analysis, the P-gp mRNA expression was increased by TNF-${\alpha}$ or IFN-${\gamma}$ treatment for 24 hr in both cell types. However, 48 hr treatment of TNF-${\alpha}$ or IFN-${\gamma}$ did not affect P-gp mRNA expression. In addition, co-treatment of TNF-${\alpha}$ and IFN-${\gamma}$ markedly increased the P-gp mRNA expression in both cells. TNF-${\alpha}$ or IFN-${\gamma}$ did not influence P-gp protein expression whatever the concentration of cytokines or duration of treatment in both cells. However, P-gp expression was increased after treatments of both cytokines together in iHBMEC cells only compared with untreated control. Furthermore, in both cell lines, TNF-${\alpha}$ or IFN-${\gamma}$ induced significant decrease of P-gp activity for 24 hr treatment. And, both cytokines combination treatment also decreased significantly P-gp activity. These results suggest that P-gp expression and function at the BBB is modulated by TNF-${\alpha}$ or/and IFN-${\gamma}$. Therefore, the distribution of P-gp depending drugs in the central nervous system can be modulated by neurological inflammatory diseases.

일부 여대생의 체성분, 식이 섭취실태 및 혈액 임상조사 (Body Composition, Food Intake and Clinical Blood Indices of Female College Students)

  • 김정희;안혜준;이상은
    • 대한지역사회영양학회지
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    • 제8권6호
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    • pp.977-985
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    • 2003
  • This study was done to evaluate the health and nutritional status of female college students in Seoul. The subjects were 63 healthy college students aged 20 to 29 years. Their body composition, dietary intakes, clinical blood indices were investigated. Their body composition was determined by means of a multifreqency bioelectrical impedance analysis. Their dietary intake was determined using 3-day record method and their nutrient intake was analyzed by Computer Aided Nutritional analysis program for professional (CAN-pro). Their hemoglobin, hematocrit, red blood cell (RBC), white blood cell (WBC) and mean corpuscular volume (MCV) were determined by semi-automated microcell counter (F-520). Their plasma total cholesterol, TG, and HDL-cholesterol levels were measured using test kits. All data were statistically analyzed by SAS PC package program. Their average consumption of calcium, iron vitamin A, vitamin B2 and niacin were 63.3%, 65.0%, 85.2%, 89.2% and 95.2% of RDA, respectively. The overall mean values of the hematological indices in the female college students were within the normal range. However anemic subjects with hemoglobin (< 12 g/dl) and hematocrit (<36%) accounted for about 20% of the subjects. The mean levels of total cholesterol, HDL-cholesterol and TG were 188.4mg/dl, 69.9mg/dl and 67.4mg/dl, respectively. The percentages of the subjects with plasma total cholesterol level (> 200mg/dl) and LDL-cholesterol (>130mg/dl) were about 41% and 30.4%, respectively. The data showed a significantly positive correlation between either body fat (%) or BMI and TG. However. there was a significantly negative correlation between either body fat (%) or BMI and HDL-cholesterol. These overall results suggest that it is necessary for college women to be educated regarding consuming more iron and vitamin C and less fat, in order to prevent iron deficiency anemia and/or cardiovascular diseases in later life.

사무직 여성의 비만요인에 따른 NK세포 활성도 관계 연구 (A Study on the Relationship between Obesity Factors and NK Cell Activity in White-Collar Females)

  • 성현호;송창섭;최광모;박창은
    • 대한임상검사과학회지
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    • 제48권3호
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    • pp.196-201
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    • 2016
  • 효과적인 비만의 중재는 대상 인구에서 가장 관련성이 있는 인구통계학적 건강 관련 및 직무 관련 요인을 고려해야 한다. 가장 강력한 비만 관련 요인은 직업군 또는 연령 범주에서 다룰 수 있다. 이 연구의 목적은 심리적인 직무 스트레스와 면역관련 인자의 혈액분석의 상관성에 대한 최신지견을 제공하는 것이다. 우리는 여성 근로자의 비만 관련 매개 변수 뿐만 아니라 자연살해세포활성(NKCA)을 평가하였다. 비만과 관련된 혈당(p<0.05)은 연령에서 유의한 차이를 보였다. 또한, 비만군과 non-비만군에서는 NK 세포의 활성에 큰 차이를 보였다(p<0.05). 단계적 다중 회귀 분석에서 NKCA는 비만 관련 요인과 밀접한 상관성을 보였다. 향후에는 면역인자들의 활성도가 증가하는 생물학적 변화를 연구해야 할 것이다.

Integrated Bioinformatics Approach Reveals Crosstalk Between Tumor Stroma and Peripheral Blood Mononuclear Cells in Breast Cancer

  • He, Lang;Wang, Dan;Wei, Na;Guo, Zheng
    • Asian Pacific Journal of Cancer Prevention
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    • 제17권3호
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    • pp.1003-1008
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    • 2016
  • Breast cancer is now the leading cause of cancer death in women worldwide. Cancer progression is driven not only by cancer cell intrinsic alterations and interactions with tumor microenvironment, but also by systemic effects. Integration of multiple profiling data may provide insights into the underlying molecular mechanisms of complex systemic processes. We performed a bioinformatic analysis of two public available microarray datasets for breast tumor stroma and peripheral blood mononuclear cells, featuring integrated transcriptomics data, protein-protein interactions (PPIs) and protein subcellular localization, to identify genes and biological pathways that contribute to dialogue between tumor stroma and the peripheral circulation. Genes of the integrin family as well as CXCR4 proved to be hub nodes of the crosstalk network and may play an important role in response to stroma-derived chemoattractants. This study pointed to potential for development of therapeutic strategies that target systemic signals travelling through the circulation and interdict tumor cell recruitment.

급성 진행성 치주염환자에서 백혈구 화학주성 기능부전에 관한 연구 (Peripheral Blood Leukocyte Chemotaxis Dysfunction in Rapidly Progressive Periodontitis)

  • 정종평;정현주;정진형
    • 대한치과의사협회지
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    • 제21권10호통권173호
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    • pp.805-812
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    • 1983
  • For the assessment of peripheral blood leukocyte chemotaxis dysfunction in rapidly progressive periodontitis, peripheral blood leukocyte of 13 patients were isolated, and tested by the method of blind well chamber technique which was recently developed by Park. Data analysis was performed from the speed of cell migration from the bottom of filter paper to the top for 18 minutes at the condition of chemo-attractant(Zymosan-activated serum). Eight of the 13 patients (62%) exhibited statistically significant inhibition of leukocyte chemotaxis, 4 patients revealed normal function and the other one patients showed elevated leukocyte chemotaxis. Four of the 8 patients in chemotaxis dysfunction showed significant inhibition of random migration and chemotaxis dysfunction both. Two of these 4 patients exhibited random migration impairment, elevation of C.D.I. and chemotaxis dysfunction. The results indicated that the progression of rapidly progressive periodontitis was influenced by the leukocyte chemotaxis dysfunction, and half of these dysfunction was associated with cell-defect of leukocyte.

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Development of ResNet-based WBC Classification Algorithm Using Super-pixel Image Segmentation

  • Lee, Kyu-Man;Kang, Soon-Ah
    • 한국컴퓨터정보학회논문지
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    • 제23권4호
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    • pp.147-153
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    • 2018
  • In this paper, we propose an efficient WBC 14-Diff classification which performs using the WBC-ResNet-152, a type of CNN model. The main point of view is to use Super-pixel for the segmentation of the image of WBC, and to use ResNet for the classification of WBC. A total of 136,164 blood image samples (224x224) were grouped for image segmentation, training, training verification, and final test performance analysis. Image segmentation using super-pixels have different number of images for each classes, so weighted average was applied and therefore image segmentation error was low at 7.23%. Using the training data-set for training 50 times, and using soft-max classifier, TPR average of 80.3% for the training set of 8,827 images was achieved. Based on this, using verification data-set of 21,437 images, 14-Diff classification TPR average of normal WBCs were at 93.4% and TPR average of abnormal WBCs were at 83.3%. The result and methodology of this research demonstrates the usefulness of artificial intelligence technology in the blood cell image classification field. WBC-ResNet-152 based morphology approach is shown to be meaningful and worthwhile method. And based on stored medical data, in-depth diagnosis and early detection of curable diseases is expected to improve the quality of treatment.

이중 박동형 생명구조장치의 혈류, 혈압, 박동성의 분석 (Analysis on the Blood F1ow, Pressure and Pulsatility of a Dual Pulsatile Extracorporeal Life Support)

  • 최성욱;민병구
    • 대한의용생체공학회:의공학회지
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    • 제28권5호
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    • pp.684-693
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    • 2007
  • Previous trials for development of a pulsatile-Extracorporeal Life Support had some defects such as insufficient blood flow, high pressure at its membrane oxygenator and the high risk of blood cell damage. To solve those problems of previous pulsatile-ECLSs, we suggest dual pulsatile blood pump structure for the new pulsatile-ECLS. Two pulsatile pumps areconnected in a parallel manner and this new structure raises the inflow capacity and efficiency and it decreases the high blood pressure at membrane oxygenator. In in-vitro experiments, The Energy Equivalent Pressure Increment(EEP inc.) was 10%, and it showed that its pulsatilty was $5{\sim}10$ times higher than other commercial ECLS In in-vivo experiments, we had applied a new pulsatile-ECLS to 30 Kg pigs and a new pulsatile-ECLS couldsupport high blood flow and pulsatility above 2 L/min, 10% EEP inc.

A Study on Chromosomal Mosaicism Detected through Cytogenetic Analysis

  • Hwang, Si-Mok;Kwon, Kyoung-Hun;Yoon, Kyung-Ah
    • 대한의생명과학회지
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    • 제17권2호
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    • pp.129-134
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    • 2011
  • Mosaicism is the presence of two or more chromosomally distinct cell lines, each seen in two or more cells. Chromosomal mosaicism presents one of the most difficult problems in prenatal cytogenetic diagnosis, requiring the differentiation of true mosaicism from pseudomosaicism. To overcome associated problems we investigated 24 cases (amniotic fluid 13 cases, abortus tissue 3 cases, peripheral blood 8 cases) in which mosaicism has been found in cytogenetic analysis. 5 cases (38.5%) of 13 amniotic fluid cells in which mosaicisms showed single cell pseudomosaicism. Chromosomal true mosaicism is found in about 0.28% (8/2,826) of amniotic fluid cell cultures. The 24 cases involved 12 cases (50%) with sex chromosomal abnormalities, 7 cases (29.2%) with autosomal structural defects, 3 cases (12.5%) with autosomal abnormalities, 2 cases (8.3%) with a supernumerary marker. Mosaicism detected in amniotic fluid may represent the true mosaicism or may pseudomosaicism. If the same chromosome abnormality is seen in more than one cell and in two different cultures, it is considered a true mosaicism, whereas single-cell abnormalities from a single culture are regarded as pseudomosaicism. In this study, we describe a mosaicism in chromosome analysis, its diagnostic problems and clinical significance.