• Korean Journal of Poultry Science
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• v.37 no.1
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• pp.1-8
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• 2010

An experiment was conducted to investigate the effect of dietary supplementation of rare earth, individually and in combination with colloidal silver on growth performance, nutrient digestibility, carcass characteristics and immune response in broiler chicks. A total of 3,872 day-old chicks were randomly allotted to four dietary treatments in a randomized complete block design. There were four pens per treatment and 242 chicks per pen. The dietary treatments were T1) CON (control diet), T2) colloidal silver (control diet+20 ppb colloidal silver), T3) rare earth elements (control diet+500 ppm rare earth), T4) colloidal silver +rare earth elements (control diet+20 ppb colloidal silver+500 ppm rare earth). There were no significant differences in feed intake and feed conversion ratio (FCR) among dietary treatments during the whole experimental period (0 to 5 weeks). Body weight gain was greatest in birds fed T3 and T4 diets (p<0.01). Apparent digestibility of dry matter, crude protein, ether extract and calcium were greatest in birds fed T4 diet (p<0.05), while apparent digestibility of phosphorus was lowest in birds fed T3 diet (p<0.05). There was no significant difference in bone strength, carcass characteristics and immune response among dietary treatments. Dry matter content of bone was greatest in birds fed T4 diet (p<0.05), and phosphorus content of bone was greater in birds fed T3 diet than in birds fed T2 and T4 diets (p<0.05). Thus, the results of this study suggest that rare earth elements supplementation, individually and in combination with colloidal silver could improve performance of broilers.

• Korean Journal of Poultry Science
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• v.35 no.3
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• pp.303-311
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• 2008

This study was conducted to investigate the effect of dietary supplementation of Cu-methionine chelate(Cu-Met) and Cu-soy proteinate(Cu-SP) on the performance, small intestinal microflora and immune response in laying hens. A total of 960 Hy-line $Brown^{(R)}$ laying hens of 39 wks old were assigned to one of the following 6 dietary treatment: control(C), antibiotic(Avilamycine 6 ppm), Cu-Met 50 and Cu-Met 100(50 and 100 ppm Cu as Cu-methionine chelate), Cu-SP 50 and Cu-SP 100(50 and 100ppm Cu as Cu-soy proteinate). Each treatment was replicated 4 times with forty birds per replication, housed in 2 birds per cages. Forty birds units were arranged according to randomized block design. Feeding trial lasted 6 wks under 16 hours lighting regimen. Hen-day and hen-house egg production of groups treated with Antibiotic and Cu supplements tended to be higher than the control with significant difference (P<0.05) shown between Cu-Me 100 and control. Egg weight was significantly (P<0.05) heavier in antibiotic and Cu-SP treatments than Cu-Met treatments but they were not significantly different from the control. Eggshell strength, egg shell thickness, egg yolk color and Haugh unit were not significantly different among treatments. There were no significant differences in leukocytes and erythrocytes in the chicken blood. But mean corpuscular hemoglobin value(MCH) was significantly (P<0.05) higher in Cu-SP 100 than antibiotic treatment. The concentrations of serum IgG and IgA were not significantly different among treatments. Copper concentration in the liver tended to increase as the level of copper supplementation increased, that of Cu-SP 100 being significantly (P<0.05) higher than those of the control and antibiotic treatment. Concentrations of iron and zinc of the liver were not significantly influenced by treatments. Populations of Cl. perfringens and Lactobacilli in the small intestinal content were significantly (P<0.05) influenced by treatments. Population of Cl. perfringens decreased and that of Lactobacilli increased in the copper supplemented groups. The result of this experiment showed that Cu-Met and Cu-SP are comparable to antibiotic in improving egg production in laying hens. Birds fed diets supplemented with Cu-SP produced heavier eggs than those fed diets with Cu-Met. There were no significant differences in the performances between 50 ppm and 100 ppm copper supplementation as organic forms.

• Korean Journal of Poultry Science
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• v.40 no.2
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• pp.97-103
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• 2013

The present study examined effects of production performance, immune activity and egg quality by raising on exercise yard in laying Hens, the results of which could be used as baseline data to enhance animal welfare and the safety of livestock products. A total of 90 Hy-line-Brown laying hens of 13 weeks old were used in the experiment for 38 weeks. The cage group (Cage group) was raised in a cage, where an area of $0.084m^2$ was assigned to two hens, while hens in the exercise yard $1.1m^2$ group ($1.1m^2$ group) was assigned to a combination of a chicken house ($0.11m^2$) and a exercise yard ($1.1m^2$) per a hen. Hens in the exercise yard $2.2m^2$ group ($2.2m^2$ group) was assigned to a combination of a chicken house ($0.11m^2$) and a exercise yard ($2.2m^2$) per a hen. Treatment was replicated 3 times with ten birds per replication. Ten birds were arranged according to randomized block design. While initial egg production rate was significantly higher in the Cage group, $1.1m^2$ group exhibited a slightly higher rate in the mid- and late-stage of the experiment, although the difference was not statistically significant. Exercise yard treatment groups exhibited a higher feed intake rate than the Cage group up until the hens were 39 weeks old (P<0.05), but the difference dissipated from that age on. The age at first egg in the exercise yard treatment groups was 16 days later than that for the Cage group (P<0.05), although differences in the quality of the eggs were not observed. The results of immune activity test showed that IgA in the exercise yard treatment groups was significantly higher than that in the Cage group (P<0.05). IgG, IgM, and corticosterone were also higher in the exercise yard treatment groups. The soil in exercise yard increased of organic matter and decreased of cation exchange capacity (CEC) in raised hens. In sum, raising hens in a exercise yard raise style decreased the rate of initial egg production, which was followed by a slight increase during the latter part of the experiment. The exercise yard raise hens' immune activity was heightened.

• Korean Journal of Poultry Science
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• v.44 no.4
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• pp.253-258
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• 2017

This study was aimed at evaluating the additivity of crude protein digestibility in mixed diets of corn and soybean meal (SBM), and comparing direct and indirect methods for evaluating crude protein (CP) digestibility. Totally, five hundred and twenty-five 18-day-old broiler chickens were grouped into 7 blocks based on body weight, and randomly allocated to 6 treatment groups in a randomized complete block design. The basal diet, diet 3, was corn-SBM-based, containing 65% corn and 28% SBM. Diets 1 and 5 contained corn and SBM, respectively, as the sole CP source. To use the difference method, 2 diets, diets 2 and 4, were prepared by mixing corn and SBM at the expense of the basal diet, respectively. Diet 2 contained 79% corn and 14% SBM, and diet 4 contained 32.5% corn and 34% SBM. To evaluate the additivity of digestibility values, the difference between measured values for the mixed diets (diets 2, 3, and 4) and predicted values calculated using the measured values for diets 1 and 5 was examined. The apparent (AID) and standardized (SID) ileal digestibility of CP in SBM differed between the direct and indirect methods; however, corresponding digestibility did not differ for corn. Additionally, the predicted and measured digestibility of both AID and SID differed in diets 2 and 3, implying that the digestibility values obtained from diets 1 and 5 were not additive for mixed diets. In conclusion, this study showed that digestibility evaluated by direct and indirect methods depends on the ingredients having different CP concentrations, and this finding may be considered to improve the accuracy of feed formulation for broiler chickens.

• Journal of Mushroom
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• v.15 no.4
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• pp.223-228
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• 2017

This study was conducted to investigate the effects of fermented spent mushroom substrates (F-SMS) of Flammulina velutipes on growth performance, carcass traits, and economic characteristics of Hanwoo steers. A yeast strain (Saccharomyces sp. UJ14) and Bacillus strain (Bacillus sp. UJ03) isolated from fresh spent mushroom substrates of Flammulina velutipes were used as probiotics to prepare F-SMS. Twenty-four Hanwoo steers (14 months old) were allocated to three dietary treatments via a randomized block design and were slaughtered at 30 months of age. These treatment groups included Control (TMR), T1 (TMR containing 10% of F-SMS) group, and T2 (TMR containing 30% of F-SMS). Body weight gain was not influenced by the experimental diets. DM and TDN intakes in the finishing period were significantly (p < 0.05) greater in group T1 than in other groups. CP intake was significantly (p < 0.05) greater in group T2 than in other groups during the whole experimental period. Among carcass traits, rib-eye area and back fat thickness tended to increase with F-SMS supplementation. The appearance rate (%) of a meat yield more than grade A was the highest in group T1. The net profits increased by 1.2% and 13.3% in groups T1 and T2, respectively. In conclusion, if a proper feeding program (including feed safety) can be ensured, spent mushroom substrates of Flammulina velutipes can prove to be a highly profitable feed source for Hanwoo steers.

• Korean Journal of Organic Agriculture
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• v.18 no.3
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• pp.419-432
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• 2010

This study was conducted to determine effects of applying cattle slurry and mixed sowing with legumes such as hairy vetch or forage pea on productivity, feed values and organic stock carrying capacity of whole crop barley and rye as winter forage crops, and to obtain organic forages together with higher soil fertility. Experimental plots consisted of 7 treatments, which were non-fertilizer, chemical fertilizer (containing phosphate and potassium: P+K), chemical fertilizer (containing nitrogen, phosphate and potassium: N+P+K), organic fertilizer, cattle slurry, cattle slurry application (mixture with hairy vetch), and cattle slurry application (mixture with forage pea) plots. Each treatment was triplicates, and seven treatments were allocated in a completely randomized block design. For whole crop barley or its mixture crops, annual dry matter (DM), crude protein (CP), and total digestible nutrients (TDN) yields of N+P+K plots were significantly (P<0.05) higher than other plots except for cattle slurry plots. The CP content of barley or its mixture crops was significantly higher tor N+P+K plot (9.8%) and mixture plots with legumes (8.6~9.7%) than those of other treatments. As 450 kg Hanwoo heifers were fed diets included 70% whole crop barley or 70% mixture crops with legumes, mixture plots are capable of raising average 1.7 to 1.8 heads/ha a year. For rye or its mixture crops, annual DM, CP, and TDN yields represented 6.9~7.1, 0.5~0.6 and 4.3~4.4 ton/ha, respectively. The N+P+K plot contained 10.8% CP, which was higher (P<0.05) than all other treatments. In case of 450 kg Hanwoo heifers fed diets included 70% rye or 70% mixture crops with legumes, mixture plots can rear average 1.9 heads/ha a year. When it was considered based on crop yields and organic stock carrying capacity, applying cattle slurry to whole crop barley or rye had the comparable yields and feed values to chemical fertilizer application. Moreover, whole crop barley and rye within cattle slurry plots had a greater combination with hairy vetch and forage pea, respectively, and their mixture crops with legumes had higher crude protein and TDN yields within cattle slurry plots. In conclusion, it would be expected that mixed sowing with legumes in the application of cattle slurry to grass crops could be substituted for imported organic grains as dietary protein sources in feeding organic livestock.

• Korean Journal of Organic Agriculture
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• v.18 no.3
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• pp.401-418
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• 2010

This experiment was selected a $\beta$-glucan producing mushroom strain and developed industrial media, and used to $\beta$-glucan as an alternative for antibiotics in weaned pigs. Yields of mycelial biomass and extracellular $\beta$-glucan from Youngji (Ganoderma lucidum) mushroom was 8.52g/L and 4.49g/L respectively. Also, we prepared optimum formula for mushroom cultivations. A total of 144 pigs ($8.6{\pm}0.9$ kg average body weight, weaned $20{\pm}3$ days of age) were allotted to 4 different treatment groups and replicated 4 times with 8 pigs per replicate in randomized complete block design. Treatments were T1) NC (negative control, basal diet), T2) PC (positive control, basal diet+0.255% antibiotics), T3) NC+0.2% $\beta$-glucan and T4) PC+0.2% $\beta$-glucan. The T2 and T4 treatments were significantly higher in feed efficiency by antibiotics group (p<0.05), however, there was no significant differences in terms of average daily gain (ADG) and average daily feed intake (ADFI) during phase I (0~14 days). In phase II (15~28 days), Pigs fed with antibiotics and $\beta$-glucan (T4) had greater ADG than other treatments (p<0.05), while no differences were observed in ADFI and feed efficiency. During the whole experiment period, the ADG of T4 treatment was higher than other treatments. Pigs fed with $\beta$-glucan (T3 and T4) had greater diarrhea score and moisture content than other treatments (p<0.05). Pigs fed with $\beta$-glucan (T3 and T4) had greater moisture content than other treatments (p<0.05). However, there was no significant differences in diarrhea score and mortality of weaned pigs. There was marginal reductions in feed cost measured feed cost per weight gain used in antibiotics and $\beta$-glucan added diet during phase I. In the second phase, the treatment supplemented with antibiotics had a significantly lower feed cost per weight gain compared to the other treatments. The results from these experiments suggests that $\beta$-glucan is likely able to improve the growth performance, and reduce feed cost although they do not have similar effects like antibiotics in weaning pigs.

• Korean Journal of Soil Science and Fertilizer
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• v.2 no.1
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• pp.31-38
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• 1969

This experiment was carried out to determine the effect of different levels of phosphate and potash firtilizers applied on yields and quality of leaf tobacco. It was done at all varying levels of phosphate and potassium fertilizers $S_0$ (non-fertilizer plot);N:P:K=0:0:0 (Kg/10a) $S_1$ (1/2 amount plot);N:P:K=10:7.5:10 (Kg/10a) $S_2$ (standard plot);N:P:K=10:15:20 (Kg/10a) $S_3$ (1.5 times plot);N:P:K=10:22.5:30(Kg/10a) $S_4$ (2.0times plot);N:P:K==10:30:40 (Kg/10a) Urea was applied as nitrogen, triple superphosphate as phosphate, potassium sulfate as potash source. The total amount of fertilizers was applied as basal dressing per one plant. This experiment was carried out by using randomized block design with 3 replication. The results obtained in terms of yields and quality are summarized as follows. 1. By increasing the amounts of phosphate and potassium fertilizers, growing status of the largest leaf length, the largest leaf width and stem size of tobacco increased in the order of $S_4>S_3>S_2>S_1>S_0$. 2. By increasing the amount of phosphate and potassium fertilizers, yield was increased in the order of $S_4>S_3>S_2>S_1>S_0$, This increase showed generally high significance among various levels of treatments. 3. The regressive equation was derived from the relationship between treatment levels and tobacco leaf yield. The most desirable treatment level for the maximum yield was estimated as the 2.87 times as much as the treatment level of phosphate and potasium fertilizers of standard plot. 4. By increased application of phosphate and potash fertilizers, the contents of $P_2O_5$ and $K_2O$ in the tobacco leaf became greater while the content of nicotine was decreased. Thus, a negative correlation between fertilizer application and nicotine content has been observed.

• Korean Journal of Psychosomatic Medicine
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• v.9 no.1
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• pp.28-36
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• 2001

Objectives : This study was undertaken to identify the clinical and psychological characteristics in patients of mental disability assessment following traumatic brain injury who had the finding with or without abnormal brain imaging study. Methods : A consecutive series of 59 patients were assessed in hospital from January 1994 to December 1998. Patients were divided into 2 groups based on normal or abnormal brain imaging and the two groups were compared in demographic characteristics, psychiatric symptoms, type of head injury, clinical psychological findings. There were 27 patients with abnormal findings and 32 with normal findings in brain imaging study. Results : Abnormal finding group in brain imaging study had significantly higher incidence of psychosis, decreased memory, decreased appetite, increased nihilistic idea, and intracranial hemorrhage. Also, abnormal finding group showed significantly lower level of performance on the block design subtest of K-WAIS and had significantly lower scores on F, hypochodriasis, depression, hysteria, psychopathic deviate, psychasthenia and schizophrenia subscale of the MMPI. Conclusion : The findings suggest that the patients undergone brain surgery due to intracranial hemorrage at that time of brain injury may have higher frequency of abnormal findings in brain imaging study, complain more cognitive and affective symptoms, and have lower the abstract concept formation and perceptual organization abilities.

• Journal of Animal Science and Technology
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• v.45 no.4
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• pp.569-576
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• 2003

An experiment was conducted to investigate the effect of microbial phytase (Natuphos$^{\circledR}$) supplementation, individually and in combination with carbohydrase enzyme complex (composed of enzymes targeted to SBM dietary components such as $\alpha$-galactosides and galactomannans; ENDO-POWER$^{\circledR}$) to corn-soy basis diet with low nutrient levels on growth performance and nutrient digestibility of growing pigs. A total of 48 crossbred weaned pigs (Landrace${\times}$Yorkshire${\times}$Duroc), 29.1$\pm$0.14 kg of initial body weight, were randomly allotted to four dietary treatments, based on weight and age, according to a Randomized Complete Block Design. There were three pens per treatment and 4 pigs per pen. The dietary treatments were 1) CON (control diet with 3,380 kcal/kg of metabolizable energy, 18.96% of crude protein, 1.10% of lysine, 0.75% of calcium and 0.35% of available phosphorus), 2) LP+NTPS (CON diet with 0.15% unit lower available P levels+0.1% phytase (500 FTU/kg; Natuphos$^{\circledR}$)), 3) LEL+ENP (CON diet with 3.0% unit lower ME and lysine levels + 0.1% carbohydrase enzyme complex (ENDO-POWER$^{\circledR}$), and 4) LPEL+ENZ (CON diet with 0.15% unit lower available P levels and 3.0% unit lower ME and lysine levels+0.1% ENDO-POWER$^{\circledR}$ and 0.1% Natuphos$^{\circledR}$ (500 FTU/kg). There was no significant difference (p〉0.05) in average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR) among dietary treatments during the whole experimental period (0 to 4 weeks). Apparent digestibility of gross energy was greater in LP+NTPS and LPEL+ENZ groups than in the LEL+ENP (p<0.05). Apparent digestibility of phosphorus was greater in LP+NTPS than in LEL+ENP (p<0.05). Dry matter excretion was lowest in LPEL+ENZ and phosphorus excretion was lowest in LP+NTPS (p<0.05). Overall, pigs fed on LPEL+ENZ group tended to have better nutrient digestibility (dry matter, gross energy, crude protein and phosphorus) than pigs fed on control group. All dietary enzyme treatment groups showed lower feed cost/body weight gain of pigs than control group. In conclusion, the results from the present study suggest that the simultaneous inclusion of phytase and carbohydrase enzyme complex to diets is advantageous with respect to reducing nutrient excretion of growing pigs and may contribute to increased economic return when added to corn-soy based growing pig diets.