• Korean Journal of Veterinary Research
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• v.27 no.1
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• pp.41-45
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• 1987

The present study was carried out to investigate the optimal conditions on blastogenesis of guinea pig lymphocytes. A microculture system in conjuction with a semiautomatic multiple sample harvester was used to study the in vitro optimal condition of guinea pig lymphocytes. Careful analysis of lymphocyte transformation to concanavalin A (Con A) and lipopolysaccharide (LPS) mitogen determined optimal conditions as: (a) 10% fetal bovine serum in RPMI-1640 medium (b) 48-hour culture period.

• Korean Journal of Veterinary Research
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• v.31 no.1
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• pp.49-53
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• 1991

The present study has been carried out to investigate the optimal condition on lymphocyte blastogenesis of rabbit lymphocytes, whole blood culture and microculture system in conjunction with a semiautomatic multiple sample harvester(SAMSH) was used to study the In vitro optimal condition of rabbit lymphocytes. Data were presented to show many variables that are involved in studying the phytohemagglutinin(PHA) and lipopolysaccharide(LPS) response of rabbit lymphocyte in a microculture system. Analysis indicated that the conditions for optimal PHA as measured by incorporation of $^3H$-TdR include: (1) use of RPMI-1640 as culture medium. (2) use of $6{\mu}g$ of PHA, per culture. (3) 48-hours culture period. Conditions for optimal stimulation with LPS mitogen were similar to those used for PHA.

• Korean Journal of Veterinary Research
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• v.26 no.2
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• pp.245-249
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• 1986

The present study has been carried out to investigate the optimal condition on the blastogenesis of guinea pig lymphocytes. A microculture system in conjunction with a semiautomatic multiple sample harvester(SAMSH) was used to study the in vito optimal condition of guinea pig lymphocytes. Data were presented to show many variables that are Involved in studying the responses of guinea pig lymphocyte in a microculture system to the stimulation of Concanavalin A(Con A) and lipopolysaccharide (LPS). Analysis indicated that the conditions for optimal Con A as measured by incorporation of $^3H$-TdR include : (1) use of RPMI-1640 as culture medium, (2) use of $6{\mu}g$ of Con A, per culture, (3) use of $1{\times}10^6$ cells per culture. Conditions for optimal stimulation with LPS mitogen were similar to those used for Con A.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.37 no.3
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• pp.179-186
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• 2024

In this paper, a modification coefficient for equivalent single degree of freedom (SDOF), considering the plasticity range of the member subjected to shock wave type of blast load, was developed. The modification coefficient for the equivalent SDOF was determined through comparison with the analysis of a multi-degree of freedom (MDOF) system. The parameters influencing the equivalent SDOF system analysis were chosen as the boundary conditions of the member and the ratio of the duration of blast load to the natural period of the member. The modification coefficient was calculated based on the elastic load-mass transformation factor. The modification coefficient curve was derived using an elliptical equation to ensure it exists between the upper and lower parameter bounds. Using the modification coefficient on examples with varying cross sections and boundary conditions reduced the SDOF analysis error rate from 15% to 3%. This study shows that using the modification coefficient significantly improves the accuracy of SDOF analysis. The modification coefficient proposed in this study can be used for blast analysis.

• Journal of the Korean Ceramic Society
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• v.51 no.2
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• pp.82-87
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• 2014

Due to the revised Korean standard KS L 5201 for Ordinary Portland Cement (OPC), the use of mixed cement has grown from 5% to 10%. This study investigates the hydration behavior of $C_3A$, asit is a cement mixture that is more commonly used than granulated blast furnace slag or limestone alone. Paste samples were prepared with either granulated blast furnace slag or limestone alone. Each sample was compared with the widely used Rietveld method with a cement mixture containing blast furnace slag or limestone. The hydration behavior of $C_3A$ in each OPC sample was assessed and results were analyzed. Granulated blast furnace slag promotes a high initial level of ettringite, but as the days passed, it promotes an increase in monosulfate, leading to cracks and expansion due to the penetration of sulfates in the solution. However, when limestone is added to the mixture, a transformation of ettringite to monosulfate occurs in the presence of the $CaCO_3$ in the limestone. It is considered that this produces hemi-carbonate and mono-carbonate and thus maintains the ettringite level.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2004.10a
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• pp.48-52
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• 2004

We have found the role of rice mitogen-activated protein kinase kinase kinase (MAPKKK), OsEDR1, as controling hypersensitive response (HR) and increased disease resistance to rice blast fungus Magnaporthe grisea. Generation of transgenic rice plants through introduction of the over-expression construct of OsEDR1 using Agrobacterium-mediated transformation results in lesion mimic phenotype. Up-regulation of defense mechanism was detected through detection of increased transcription level of rice PBZ1 and PR1a. Inoculation of rice blast fungus on the lesion mimic transgenic lines displayed significantly increased resistance. The disease symptoms were arrested like HR responses which are commonly detected in the incompatible interactions. High accumulation of phenolic compounds around developing lesions was detected under UV light. There was variation among transgenic lines on the timing of lesion progression as well as the lesion numbers on the rice leaves. Transgenic lines with few lesions also show increased resistance as well as equal amount of grain yields compared to that of wild type rice cultivar Nipponbare. This is the first report of the MAPKKK as a positive regulator molecule on defense mechanism through inducing HR-like cell death lesion mimic phenotype. The application of OsEDR1 is highly expected for the development of resistant cultivars against rice pathogens.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.19 no.8
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• pp.81-87
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• 2020

A stable rotational-to-linear motion transformation structure using a driving mechanism with 2 degrees of freedom was developed for an orthogonal mechanism to prevent the interference of each axis in 2D motion. In this mechanism, a step motor was used for precise position control. This structure was developed to maneuver workparts in micro particle blast machining experiments. To determine the real-time performance of micro particle blast machining, the control, input, and output were operated simultaneously and precise position control was implemented, using a timer interrupt with multiple execution codes. The two step motors obtained precise position control by removing backlash with a ball-screw mechanism. The device has menu-type control codes for user-friendliness, and real-time sequence control was simultaneously adopted for user control input.

• Proceedings of the Korea Society for Energy Engineering kosee Conference
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• 1998.05a
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• pp.87-94
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• 1998

폭발현상(explosion phenomena)이 항상 연소(combustion)를 수반하는 것도 아니고, 연소현상이 항상 폭발적으로 일어나는 것이 아님에도 불구하고 많은 사람들은 폭발과 연소 사이에 밀접한 관계가 있는 것으로 생각하고 있다. 일반적으로 폭발이라고 하면 우선 큰 소리와 건물이나 실내의 파괴를 연상한다. 폭발 시에 발생하는 큰소리, 이른바 폭발음은 공기 중을 전파하는 압력파(blast wave)에 의한 것이고 건물이나 실내 파괴는 그들의 내부압력 상승에 의한 것이다. 그러므로 폭발현상은 압력상승과 불가분하다고 생각해도 된다. (중략)

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1998.04a
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• pp.377-380
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• 1998

Basting vibration and air blast were measured by particle velocity and overpressure as well as environmental unit, dB(V) and dB(A). Variables for vibration and noise show good relationship and proportional equations are suggested. But there are some variations, and it means that predictions by design variables are not valid for human damage estimation or limit application of regulations.

• The Journal of the Korean Society for Microbiology
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• v.18 no.1
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• pp.59-65
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• 1983

To investigate the effects of placental serum to in vitro culture of the normal human lymphocytes the peripheral blood lymphocytes were isolated by ficoll-hypaque separation method in 20 healthy human adults. The blast transformation respone of lymphocytes to mitogens were observed by stimulation with PHA($25{\mu}g/ml$) and Con A($25{\mu}g/ml$) using RPMI 1640 media containing 20% placental serum(PS), tetal calf serum(FCS), or humans AB serum(AB). And one-way mixed lymphocyte culture test was performed between these unrelated person compounded into stimulators and responders to investigate the effect of placental serum. The following results were obtained. 1. In 20 experimental cases, these were no significant diffenence between FCS, AB, and PS in untreated control cultures. 2. In PHA-treated cultures, whereas the blast transformation rate of the FCS groups and AB groups were $40.8{\pm}4.3%$ and $44.6{\pm}4.3%$ respectively, that of PS groups were $21.7{\pm}3.4%$, Similar results were obtained in Con A-meated cultures. Therefore, placental serum inhibited the mitogenic response of lymphocytes significantly. 3. In MLC tests, stimulation index of the FCS groups and AB groups were $18.5{\pm}3.5$ and $20.1{\pm}3.3$ respectively. But placental serum inhibited MLC response of lymphocytes significantly($7.4{\pm}1.9$).