• Korean journal of applied entomology
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• v.48 no.3
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• pp.327-333
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• 2009

Biological Characteristic of Obolodiplosis robiniae and insecticidal activity of some insecticides against larvae of O. robiniae were investigated. Egg was in oval shape, and its color was light orange and became red when close to hatch. Length of the major axis and the minor axis of egg was 0.4 mm and 0.1 mm, respectively. Larval color was milky and size was 2.6 mm. Pupa was deep brown and its size was about 3.2 mm. Wing and abdomen of adult was black and reddish, respectively. Size of female adult was about 3.3 mm, and larger than male adult. Number of eggs in the ovary was $192.3{\pm}50.7$. First emergence was from late April to late May, and second from late May to late June. Third emergence was from late June to late July. Newly emerged adult escaped from soil, and second and third emerged adult escaped directly from gall of Robinia pseudoacacia leaf. Egg parasitoid was identified as Platygaster robiniae and parasite rate was 51.6%. Among test insecticides, imidacloprid 10% WP and thiacoprid 10% FL showed very strong insecticidal activity against larvae of O. robiniae at 48h later after treatment.

• International Journal of Oral Biology
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• v.45 no.3
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• pp.107-114
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• 2020

Acacetin, which is present in damiana (Turnera diffusa) and black locust (Robinia pseudoacacia), has several pharmacologic activities such as antioxidant, anti-inflammatory, and anti-proliferative effects on cancer cells. However, the effect of acacetin on head and neck cancers has not been clearly established. This study aimed to examine the effects of acacetin on cell growth and apoptosis induction in FaDu human pharyngeal carcinoma cells. These were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, Live/Dead cell assay, 4',6-diamidino-2-phenylindole dihydrochloride staining, caspase-3 and caspase-7 activation assay, and immunoblotting in FaDu cells. Acacetin induced FaDu cell death in a dose-dependent manner, with an estimated IC₅₀ value of 41.9 µM, without affecting the viability of L-929 mouse fibroblasts as normal cells. Acacetin treatment resulted in nuclear condensation in the FaDu cells. It promoted the proteolytic cleavage of procaspase-3, -7, -8, and -9 with increasing amounts of the cleaved caspase isoforms in FaDu cells. Acacetin-induced apoptosis in FaDu cells was mediated by the expression of Fas and activation of caspase-8, caspase-3, and poly (ADP-ribose) polymerase. Immunoblotting showed downregulation of the anti-apoptotic mitochondrial proteins Bcl-2 and Bcl-xL, but upregulation of the mitochondria-dependent pro-apoptotic proteins Bax and Badin FaDu cells after acacetin treatment. These findings indicate that acacetin inhibits cell proliferation and induces apoptotic cell death in FaDu human pharyngeal carcinoma cells via both the death receptor-mediated extrinsic apoptotic pathway and the mitochondria-mediated intrinsic apoptotic pathway.

• Journal of Korea Foresty Energy
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• v.20 no.2
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• pp.9-19
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• 2001

This study was carried out to investigate natural insectifuge materials from tree extractives in order to substitute for organic synthetic insecticides for food wrapping corrugated board. Tree samples were collected, extracted, fractionated with hexane, $CH_2Cl_2$, ethylacetate(EtOAc) and $H_2O$, and then freezed dried for further study. EtOAc or $H_2O$ fractions were chromatographed on a Sephadex LH-20 column for isolation and purification, and the isolated compounds were characterized by spectroscopic tools such as NMR and MS. Crude extractives of EtOAc and $H_2O$ fractions were added to the printing ink for corrugated board with the concentration of 2% or 3% based on the weight of the ink, then the prepared ink was printed on the corrugated board to be used for evasion test using larva of indian meal moth(Plodia interpunctella(Hubner)). Robtin, dihydrorobinetin and leucorobinetinidin were isolated from the wood extractives of black locust(Robinia pseudoacacia) and the bark of poplar(Populus alba $\times$ glandulosa) contained many kinds of compounds such as (+)-catechin, naringenin, aromadendrin, eriodictyol, sakuranetin and its glycoside, taxifolin, neosaturanin, salireposide, p-coumaric acid and aesculin. Much of (+)-catechin was isolated from the bark extractives of willow(Salix koreensis) in addition to (+)-gallocatechin and p-coumaric acid and the bark of weeping willow(Salix babylonica) also contained (+)-catechin, (+)-gallocatechin, dihydromyricetin and myricetin.

• Journal of Ecology and Environment
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• v.31 no.1
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• pp.23-29
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• 2008

The effectiveness of rehabilitation programs for coal mining spoils in Samcheok, Jeongsun, and Mungyung were evaluated based on the physicochemical properties of soil in the rehabilitated areas. These spoils were reclaimed by introducing plants such as black locust (Robinia pseudoacacia), pitch pine (Pinus rigida), birch (Betula platyphylla var. japonica), alder (Alnus hirsuta), bush clover (Lespedeza cyrtobotrya), and grass (Lolium perenne) in planting beds covered with forest soil. In the surface soil, the pH, organic matter, total N, available P, and exchangeable Ca showed significant changes over the years after reclamation. The pH and exchangeable Ca content decreased exponentially over time, whereas organic matter increased linearly and total N and available P increased exponentially. Changes in the physicochemical properties of subsurface soils displayed a different pattern. There were significant changes over time in the organic matter, available P, and exchangeable Ca and Mg contents of the soil. Organic matter increased logarithmically with years since rehabilitation and available P increased exponentially. Meanwhile, exchangeable Ca decreased exponentially, and Mg decreased logarithmically. The changes in the subsurface soil were not as dramatic as those in the surface soil. This result suggests that the ameliorating effects of the establishment and growth of plants more pronounced on the surface soil layer. Stand ordination data showed different relationships with time since rehabilitation in the early and later stages of the rehabilitation process. In the early stages of rehabilitation, stands tended to be arranged in the order of reclamation age. However, in the later stages, there was not a clear relationship between reclamation age and vegetation characteristics. This result suggests that soil amelioration is required for the early stages, after which an autogenic effect becomes more prominent as the vegetation becomes better established.

• Current Research on Agriculture and Life Sciences
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• v.19
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• pp.17-22
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• 2001

The study was conducted to establish the method of performance test of honeybee queens under Korean environment. Colony performances of Apis mellifera were tested based on wintering ability, colony population and honey yield in Sangju, Korea from November, 1999 to October, 2001. The results of performance-testing on the honeybee colonies are summarized as follows: The colony weights measured before and after wintering in 1999~2000 season were averaged $22.6{\pm}2.6kg$ and $20.1{\pm}2.6kg$, respectively, showing the decrease of $2.5{\pm}0.7kg$. During 2000~2001 winter season, the colony weight before wintering was $22.6{\pm}2.3kg$ and was decreased by $2.3{\pm}0.6kg$. The number of combs well covered with bees was $13.1{\pm}4.6$ as measured on the 22nd of May. 2000 and $16.0{\pm}3.8$ on the 21st of May. 2001, respectively. The rate of supering colonies were 50% and 98.5% in 2000 and 2001, respectively. The amount of honey production was $12.7{\pm}8.6kg$ per colony in 2000, and $14.2{\pm}7.6kg$ per colony with unselected queens and $23.5{\pm}4.9kg$ per colony with selected-tested queens in 2001, which were harvested two times during main nectar flow season of black locust, Robinia peseudoacasia.

• Journal of the Korean Institute of Landscape Architecture
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• v.49 no.2
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• pp.74-88
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• 2021

In the present study, the characteristics and changes in the vegetation of Mt. Cheongryang, Inchon, were examined to identify and determine appropriate ways to restore the health of the urban forest and to preserve its vegetation. The vegetation of the community of Quercus mongolica (Mongolian oak) on Mt. Cheongryang appeared to decrease in response to the control of the wilt disease of oak trees. The communities of Sorbus alnifolia (Korean mountain ash) and Styrax japonicus (Snowbell tree) have increased. Pinus rigida (Pitch Pine) had its overall territory decrease, but the current state of the Pinus rigida (Pitch Pine) was estimated to be stable due to its dominance as a tree layer species. In regards to Robinia pseudoacacia (Black Locust), the urbanized species of Sorbus alnifolia (Korean mountain ash), and Styrax japonicus (Snowbell tree), their areas have increased with the appearance of Magnolia obovate (Whiteleaf Japanese Magnolia). The biodiversity of Mt. Cheongryang has decreased by simplifying species in the tree layer and understory species thereof, and the initial success of species in marginal areas has increased. The absence of potential succession was attributed to the termination of ecological succession; thereby, the current vegetation structure was concluded to be remaining as it is for the time being. Soil texture in the mountain primarily consisted of sandy loam or loamy sand; the pH of the soil was in the range 4.26-4.86, rendering a mean pH of 4.59. The content of organic matter (O.M.) appeared having a distributing range of 2.18-9.60%, rendering a mean value of 4.33%. To promote species diversity, several methods are suggested, such as prevention of soil acidification, selecting nationally-grown trees from moist soil or valleys for afforestation, preventing species appearing due to urbanization or excessive growth, protecting the understory vegetation and species with hygropreference, and managing the forest to maintain a multi-layered vegetation structure.

• Animal Bioscience
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• v.35 no.2_spc
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• pp.332-346
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• 2022

Shortage of protein feed resources is the major challenge to the world farm animal industry. Insects are known as an alternative protein source for poultry. A wide range of insects are available for use in poultry diets. Insect larvae thrive in manure, and organic waste, and produce antimicrobial peptides to protect themselves from microbial infections, and additionally these peptides might also be functional in poultry feed. The feed containing antimicrobial peptides can improve the growth performance, nutrient digestibility, intestinal health, and immune function in poultry. Insect meal contains a higher amount of essential amino acids compared to conventional feedstuffs. Black soldier fly, mealworm, housefly, cricket/Grasshopper/Locust (Orthoptera), silkworm, and earthworm are the commonly used insect meals in broiler and laying hen diets. This paper summarizes the nutrient profiles of the insect meals and reviews their efficacy when included in poultry diets. Due to the differences in insect meal products, and breeds of poultry, inconsistent results were noticed among studies. The main challenge for proper utilization, and the promising prospect of insect meal in poultry diet are also addressed in the paper. To fully exploit insect meal as an alternative protein resource, and exert their functional effects, modes of action need to be understood. With the emergence of more accurate and reliable studies, insect meals will undoubtedly play more important role in poultry feed industry.

• Journal of Korean Society for Atmospheric Environment
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• v.10 no.E
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• pp.371-386
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• 1994

Ecological examinations of the radial growth Patterns of pine trees(Pinus densiflora Sieb. et Zucc) growing on Mt. Namsan in central Seoul were made to test a Proposition that the pine trees decline due to the influence of air pollution and acid rain, which was proposed by some researchers in Korea, and the potential effects of current level of air pollutants to the growth of the Pine trees in central Seoul have been speculated. Tree-rings of 40 trees sampled at 3 sites of Mt. Namsan were prepared and examined using a Computer-aided Tree-Ring Measuring System at Kookmin University, Korea. Air Pollutant data collected by the Ministry of Environment( MOE ) and the Forestry Research Institute(FRI) were used to infer the general conditions of the environment. Correlation analysis was applied to the data set of tree growth and the other environmental factors. General information derived from the close examination of the tree-rings and the data on air pollution, drought and the other biological conditions suggested that the growth of the pine trees was severely affected by the occurrence of drought(climatic variation), the prevalence of the pine leaf gall midges(insects), and the suppression by the black locust trees(Robinia pseudo-acacia L.) (competition among trees). While the current condition of air pollution in Seoul cannot be categorized as good, the concentrations of air pollutants are not so high as to cause acute damages to the trees. In addition, while the data of rain acidity showed episodic low PHs of under 4.0, the average of them is far less acidic than those which were observed in either northeastern United States or central Europe, where the decline of trees were not solely attributed to any of the air pollutants. Considering the sequential facts that one of the most important environmental factors that affect the growth of trees is weather condition of the forest that the proposition of the decline of the pine trees was made without careful examination of the growth patterns and past growth history of them as well as the complex influences of many other factors including the weather conditions to the growth of trees, and that no objective explanation has been made on the causal relationships between the current condition of air pollution and the growth of the trees, such a proposition should be evaluated as invalid for the explanation of tree growth on Mt. Namsan in central Seoul, Korea. The author evaluates the factors of air pollution (including acid rain) as the predisposing factors, which may have the Potentials to chronically affect the tree growth at the forest ecosystem on Mt. Namsan for a long period of time. Ecosystem ecological studies should be further carried out to carefully explain both the functional and the structural aspects of the ecosystem processes, which include the biogeochemistry and the long-term changes of soil conditions as well as the growth of the other tree species on the mountain.