• 한국응용곤충학회지
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• 제33권2호
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• pp.60-65
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• 1994

침엽수 계통의 많은 수종은 가해하는 솔잎벌(Nesodiprion japonicus{{{{( { M}_{ ARLATT}) }}}}) 생태중 생활사에 관하여 조사한 것을 요약하면 다음과 같다. 성충은 1년 2회 발생하였으며, 암컷과 수컷이 같은 색 (검정)이고, 암컷의 몸체는 수컷보다 굵고 크며 촉각은 빗살모양으로 수컷이 암컷보다 크다. 성충의 수명은 3~4일, 우화율은 92% 성비는 6(female): 4(male)로 나타났다. 성충은 침엽의 조직내에 1입씩 산란하며 산란된 배 입은 각색으로 변한다. 란 기간은 $14.3\pm$1.2일이었으며, 란의모양은 짧은 바나나형이고 등황색을 띠며 크기는 $2.4\pm$0.2mm 이다. 유충기간은 $30.3\pm$2.1일이고, 크기는 $20.1\pm$2.2mm이다. 두정에는 흑빈이 있고 몸체는 광택이 있는 녹생으로서 전후야란은 황색을 띠고 있다. 노숙유충이 되면 녹색이 연녹색으로 변하고 두정의 흑반도 없어진 후 1회 발생 때의 유충은 솔잎의 기부에 , 2회 대의 유충은 지피물 사이에 고치를 짓고 융화한다. 전용기는 $14.3\pm$2.0일이였고, 크기느 $10.1\pm$0.2mm이다. 용기간은 $12.2\pm$1.3일이었으며, 크기는 $10.1\pm$0.2mm이다.

• Toxicological Research
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• 제31권4호
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• pp.403-414
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• 2015

Lithospermum erythrorhizon has long been used as a traditional oriental medicine. In this study, the acute and 28-day subacute oral dose toxicity studies of hexane extracts of the roots of L. erythrorhizon (LEH) were performed in Sprague-Dawley rats. In the acute toxicity study, LEH was administered once orally to 5 male and 5 female rats at dose levels of 500, 1,000, and 2,000 mg/kg. Mortality, clinical signs, and body weight changes were monitored for 14 days. Salivation, soft stool, soiled perineal region, compound-colored stool, chromaturia and a decrease in body weight were observed in the extract-treated groups, and no deaths occurred during the study. Therefore, the approximate lethal dose (ALD) of LEH in male and female rats was higher than 2,000 mg/kg. In the subacute toxicity study, LEH was administered orally to male and female rats for 28 days at dose levels of 25, 100, and 400 mg/kg/day. There was no LEH-related toxic effect in the body weight, food consumption, ophthalmology, hematology, clinical chemistry and organ weights. Compound-colored (black) stool, chromaturia and increased protein, ketone bodies, bilirubin and occult blood in urine were observed in the male and female rats treated with the test substance. In addition, the necropsy revealed dark red discoloration of the kidneys, and the histopathological examination showed presence of red brown pigment or increased hyaline droplets in the renal tubules of the renal cortex. However, there were no test substance-related toxic effects in the hematology and clinical chemistry, and no morphological changes were observed in the histopathological examination of the kidneys. Therefore, it was determined that there was no significant toxicity because the changes observed were caused by the intrinsic color of the test substance. These results suggest that the no-observed-adverse-effect Level (NOAEL) of LEH is greater than 400 mg/kg/day in both sexes.

• 대한수의학회지
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• 제8권1호
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• pp.45-53
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• 1968

1. The albumin, ${\beta}-globurin$ and ${\gamma}-globurin$ fractions of non-vaccinated bovine serum (Control) int declined, and a total protein and ${\alpha}-globurin$ level are advanced on the reversible rather than of vaccinated immumized bovine serum. (Table 5.) 2. Some few exists the to bring about changed in the individuals and sexual in a vaccinated bovine serum, however, Male serum globurin fractions are higher than Female globurin fractions percentage. (Table 1 and 3.) 3. Albumin fractions are Age-ablly variable, so that, younger's are rather lower than adult's such reported as in the another litratures. 4. In the monthly analysis of immunized bovine serum the first week to at dulation for third weeks were slowly advanced as variablly in serum-protein fractions of ater by the Black-leg No. 2 vaccination, then, albumin fractions were illustated as maximum ratio (42.73 3.49%) and increased much as 14.9% more than non-vaccinates, and ${\alpha}-globurin$ fractions indicated the minimum ratio(15.11 2.35%) at for 4th month after vaccination decrease much as about 7.71 % rather non-vaccinated normal bovine serum. (Table 4. and Fig 3.) Next, continuous advanced the ${\beta}-globurin$ fractions at first month as primary crisis in a diagram, and indicated the maximum ratio at 8th months as the second crieir on the its diagram of after vaccinati on, however, few changed in non-vaccinated bovine serum. (Figs 4.) Especially, ${\gamma}-globurin$ fractions are advanced the maximum ratio as 41. 45% 4.48% anp advanced to be widely range much as about 22.55% more than control serum at 5th months of after the vaccination. That is one of the most considerable evalution in Black-leg No. 2 vaccination to Korean calevs as great presence of the maximum immune antibodies at for 5th month after the vaccination. (Table 4. and Fig 4.) 5. In the relationship between vaccinated rabbit and Korean calves, serum protein fraractions were to be changed within the 3 weeks, so that albumin fractions of vaccinated immune bovine serum are increased as directly ratio, while vaccinated rabbit immunized serum showed the decrease as to reciprocal ratio. Although, conclude that ${\gamma}-globurin$ fractions are increased gradually by and large on the vaccinated immune bovine serum(B. P. S.) and vaccinated immune rabbit serum(R. P. S.) together. (Table 6).

• 한국환경생태학회지
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• 제22권4호
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• pp.427-434
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• 2008

2004년 10월부터 2006년 12월까지 지리산에 방사한 반달가슴곰 7개체를 무선추적하여 연간 행동권 크기를 분석한 결과, 95% MCP에서 반달가슴곰 암컷은 71.66$km^2$, 수컷은 90.20$km^2$로 분석되었다. 계절별 행동권 크기는 암컷곰이 봄 19.8$km^2$, 여름 30.48$km^2$, 가을 22.63$km^2$, 겨울 0.22$km^2$이었고, 수컷곰은 봄 33.47$km^2$, 여름 20.26$km^2$, 가을 23.23$km^2$, 겨울 0.78$km^2$이었다(F=4.193, P<0.05). 반달가슴곰의 행동권역중 약 38% 정도가 중첩되었고, 암컷간 12.5%, 수컷간 22.4% 정도가 중첩되었다. 서식지 평균 해발고도는 봄 744$\pm$236m, 여름 682$\pm$253m, 가을 937$\pm$218m, 겨울 975$\pm$137m로 분석되었다(F=8.526, P<0.001). 반달가슴곰의 행동권 크기와 위치는 주로 먹이의 분포와 생산량과 밀접한 관계가 있으며, 계절별 서식 환경특성, 연령 등에 따라서도 차이가 있음을 알 수 있었다.

• Parasites, Hosts and Diseases
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• 제55권1호
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• pp.89-93
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• 2017

The aim of this study was to determine the seroprevalence and risk factors of fascioliasis in yaks, Bos grunniens, from 3 counties of Gansu Province in China. A total of 1,584 serum samples, including 974 samples from white yaks from Tianzhu, 464 from black yaks from Maqu, and 146 from black yaks from Luqu County, were collected and analyzed using ELISA to detect IgG antibodies against Fasciola hepatica. The overall F. hepatica seroprevalence was 28.7% (454/1,584), with 29.2% in white yaks (284/974) and 27.9% in black yaks (170/610). The seroprevalence of F. hepatica in yaks from Tianzhu, Luqu, and Maqu was 29.2%, 22.6%, and 29.5%, respectively. Female yaks (30.9%) had higher F. hepatica seroprevalence than male yaks (23.4%). Also, F. hepatica seroprevalence varied by different age group from 24.1% to 33.8%. Further, the seroprevalence ranged from 21.8% to 39.1% over different seasons. Interestingly, the season and age of yaks were associated with F. hepatica infection in yaks in the investigated areas. These findings provided a basis for further studies on this disease in yaks from 3 counties of Gansu Province in northwestern China, which may ultimately support the development of effective control strategies of fascioliasis in these areas.

• Reproductive and Developmental Biology
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• 제35권1호
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• pp.77-83
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• 2011

One-step dilution and direct transfer would be a practical technique for the field application of frozen embryo. This study was to examine whether Jeju Black Cattle (JBC, Korean Cattle) can be successfully cloned from vitrified and one-tep diluted somatic cell nuclear transfer (SCNT) blastocyst after direct transfer. For vitrification, JBC-SCNT blastocysts were serially exposed in glycerol (G) and ethylene glycol (EG) mixtures [10%, (v/v) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.] which is diluted in 10% FBS added D-PBS. And then SCNT blastocysts were loaded in 0.25 ml mini straw, placed in cold nitrogen vapor for 3 min. and then plunged into $LN_2$. One-step dilution in straw was done in $25^{\circ}C$ water for 1 min, by placing vertically in the state of plugged-end up and down for 0.5 min, respectively. When in vitro developmental capacity of vitrified SCNT blastocyst was examined at 48 h after one-step dilution, hatched rate (56.4%) was slightly lower than that of control group (62.5%). In field trial, when the vitrified-thawed SCNT blastocysts were transferred into uterus of synchronized 5 recipients, a cloned female JBC was delivered by natural birth on day 299 and healthy at present. In addition, when the short tandem repeat marker analysis of the cloned JBC was evaluated, microsatellite loci of 11 numbers was perfectly matched genotype with donor cell (BK94-14). This study suggested that our developed vitrification and one-step dilution technique can be applied effectively on field trial for cloned animal production, which is even no longer in existence.

• 한국양식학회지
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• 제14권3호
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• pp.173-180
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• 2001

광주기와 수온의 인위제어에 따른 조피볼락, S. schlegeli의 교미 및 출산 조절을 번식생물학적으로 조사하였다. 어미의 출산유도 사육실험은 자연조건의 대조구와 광.수온 조절의 실험구로 나누어 사육하였다. 실험구에 있어서 광주기 조절은 교미시기에 9L:15D, 출산시기에는 14L:10D로 조절하였다. 수온 조절은 교미시기 12~13$^{\circ}C$, 출산시기에 14~15$^{\circ}C$로 조절하였다. 수컷의 GSI는 1996년 4월에서 8월까지 딱 0.12로 낮은 값을 유지하였고, 대조구는 11월에 1.36$\pm$0.10, 실험구에서는 10월에 1.27$\pm$0.05로 각각 최대값에 달하였다. 암컷의 GSI는 1996년 1월에서 11월까지 약 1.82로 낮은 값을 유지하였고, 대로구는 4월에 46.5$\pm$5.72, 실험구는 1997년 2월에 42.0$\pm$4.24로 각각 최대값을 가졌다. 광주기와 수온을 조절한 실험구는 정소와 난소의발달 양상이 대조구와 유사하였으나, 교미와 출산은 2~3개월 앞당길 수 있었다.

• 한방안이비인후피부과학회지
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• 제12권2호
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• pp.212-221
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• 1999

Tongue examination is the most unique and important diagnostic method of oriental medicine. It reveals patients condition and give some information about direction of therapy and background of disease as well as produces clue of duration of disease changing that grasps it from outside. The aims of this study show that results of tongue examination is related to somewhat special pattern. we study 27 patients(age of means: 63.01 years old, male: 14, female: 13) who come to Wonkwang University Oriental-Medicine Hospital at Chonju with diabetes mellitus or diabetes mellitus complications. Tongue colors of this study result in pale-red 8 cases, pale 6 cases, red 6 cases, crimson 5 cases, blue-green-purple 2 cases and tongue fur of this study result in white-fur 16 cases, yellow-fur 7 cases, black-fur 1 case, none-fur 3 cases. And the condition of tongue fur with grimy and thin result in thin-white-fur 12 cases, white-grimy-fur 4 cases, thin-yellow-fur 5 cases, grimy-yellow-fur 2 cases, black-fur 1 case. Means level of fasting glucose during 7 days was $223.24{\pm}32.l7mg/dl$ and postprandial 2hours was $286.37{\pm}24.54mg/dl$ There were no changes in tongue body and tongue color but changes occur in tongue fur with 9cases in this period. 5 cases of patient make a difference between FBS(fasting blood glucose) and postprandial blood glucose level more than l00mg/dl (2 cases of patient with thin-white-fur gradually turned to slight-yellow-fur. There were no changes in 2 cases of patient with crimson-none-fur and pale-white-fur. 1 case of patient with slimy-yellow-fur turned to black fur.) 11 cases of patient had lesser than $10\%$ hemoglobin $A_lC$ and tongue color of these patient were pale-red 5cases, pale 2 cases, red 2 cases, crimson 2 cases. 16 cases of patient had more than $10\%$ Hemoglobin $A_1C$ and tongue color of these patients were pale-red 3 cases, pale 4 cases, red 4 cases, crimson 3 cases, blue 2 cases. This result shows that quantity of Hemoglobin $A_1C$ make a somewhat role in tongue color. The above results show that tongue color, fur color, condition and change of fur in diabetes mellitus patients is various in pale-red, pale, crimson, none-fur. So it is difficult to give an exact diagnosis on pathology of diabetes mellitus only with tongue examination because there are a little matches between blood glucose level, prevalence-period, short-term blood glucose regulation and tongue and fur colors.

• 한국축산식품학회지
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• 제43권4호
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• pp.639-658
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• 2023

Although goat meat has several health benefits than other red meats but comsumers reluctant it due to its unpleasant flavor. This study aimed to investigate the odorant of goat meat as well as compare the quality traits of meat regarding sex status. The loin meats [non-castrated male (NCM), castrated male (CM), and female (FM)] were collected and stored at 4℃ in a laboratory refrigerator and analyzed on the 1^st, 5^th, and 8^th consecutive days. The moisture content was the lowest and the protein content was highest in FM (p<0.05). Fat and ash content in NCM and FM were similar while lowest in CM. The CIE L^* was significantly higher in NCM, but there were no significant differences of CIE a^* and CIE b^* within groups at initial day. The color intensity increased on the 5^th storage day and decreased again after the 8^th storage day, except in NCM. NCM displayed the highest thiobarbituric acid reactive substances value (p<0.05), whereas CM displayed a higher pH value than other groups throughout the storage period. Indole levels were the highest in NCM (0.031 mg/kg); however, skatole levels were not significant differences across all treatments (p>0.05). No significant difference was observed in the fatty acid composition between NCM and CM (p>0.05), whereas a significant difference was observed in FM (p<0.05). Most of the water-soluble metabolites showed significant differences between sexes. Overall, sex status effects on quality properties of meat and castration can improve the overall sensory acceptance by reducing goaty flavor of Korean native black goat meat.

• 한국임상수의학회지
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• 제31권3호
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• pp.246-249
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• 2014

9살의 중성화하지 않은 암컷 시츄가 복통과 복부 팽만을 주증으로 내원했다. 보호자는 개가 태어난 이후로 발정과 관련한 출혈을 단 한번도 관찰하지 못 했다. 초음파 검사 상에서 분절된 생식기계의 확장이 관찰되었다. 생식기계의 종괴를 확인하고, 난소 자궁 질 적출술을 실시하였다. 육안적으로 관찰했을 때, 질은 확장되어 있었고 고형의 짙은 초록색의 물질로 차있었다. 수술 결과와 질조영술을 바탕으로하여 처녀막 잔존증으로 진단하였다. 수술 한달 후, 봉합한 부위와 질의 막혀있는 부분 사이에 잔존성 질축농증이 발생하였다. 잔존된 처녀막은 내시경으로 제거하고, 세척하였으나, 복막염과 패혈증이 발생으로 폐사하였다.