• 한국식품조리과학회지
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• 제12권1호
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• pp.115-121
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• 1996

Ixeris dentata Nakai (Compositae) is a perennial herb and the young shoots when prepared properly are edible as a bitter appetizing vegetable in the early spring. The whole plant has been used as a folk medicine in treating diabetes in Korea. As a part of our studies on bitter substances, we have investigated the bitterness of the plant through a taste-guided fractionation method. Bitter substances were extracted by the solvent fractionation and the bitterness was evaluated by 10 trained panelists consisted with graduate students of Dept. of Foods and Nutrition. It has been revealed that the bitter compounds were distributed throughout the extracting fractions and the higher intensity of the bitterness was seen in the n-BuOH fractions. The bitter compounds were subfractionated by the silica gel column chromatography and the bitterness was evaluated by the trained panelists. The subfractions which were indicated as the bitter compounds will be further studied to isolate the compounds. The H$_2$O fractions were grouped according to the molecular weight through the ultrafiltration and the group with 1,000 < m.w. < 30,000 was evaluated as the most intense bitter subfraction.

• 한국식품저장유통학회지
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• 제11권1호
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• pp.38-41
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• 2004

유자 착즙액에 naringiase와 탁주용 당화 효소 3% 처리 결과 naringin과 hesperidin의 함량이 61.94 mg%, 9.98 mg%에서 각각 8.43 mg%, 0.06 mg%와 6.58 mg%, 1.11 mg% 감소하였다. Hunter value를 측정한 결과 적색도/녹색도(a값)는 효소처리구 모두 대조구보다 높게 나타났고, 황색도(b값)와 명도(L값)는 탁주용 당화 효소가 가장 높았다. 복합 효소 처리후 실제 관능적으로 고미제거에 대한 효과와 선호도를 조사한 결과는 각 제조사별 복합효소를 처리한 시료구가 대조구보다 전체적으로 선호도가 높았고, naringin의 함량이 적을수록 기호도는 증가하였다. 호소의 최적 첨가량은 3% 내외가 바람직한 것으로 사료된다.

• 한국식품과학회지
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• 제27권5호
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• pp.646-651
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• 1995

순수 물질 수용액의 표면장력치 측정과 JND를 통한 맛 관능 분석에 의해 쓴 맛 물질의 표면 장력과 쓴 맛 간의 상관성을 연구한 결과, 모든 쓴 맛 물질 수용액은 용액의 농도가 증가함에 따라 표면 장력이 감소되어 Szyszkowski 방정식과 Techplot program으로 표면 장력 감소 곡선을 수학적으로 나타낼 수 있었고 또한 관능 분석에 의한 JND 맛 곡선도 같은 방법으로 묘사하여 상호 비교됨을 알 수 있었다. 또한 측정된 모든 쓴 맛 성분들에 대하여 표면 장력 곡선으로부터 상수 a, b 값을, 맛 곡선으로부터 상수 A, B 값을 계산하여 쓴 맛 인지역치 $log\;C_{1}$과 평형 상수 b 값 간의 음의 상관 관계식을 구하였다. 또한 쓴 맛 인지역치 농도 $log\;C_{1}$과 기계적인 측정치로 산출된 parameter들의 상관성을 PV-wave 프로그램을 이용해서 3차원적으로 비교한 $log\;C_{1}$/a/b와 $log\;C_{1}$/A/B간의 상관관계는 주목할 만하며 이는 용액과 기체간의 계면 system에서의 계면 흡착 모델이 구강 내에서의 조직과 용액 간의 관능적 모델에도 적용됨을 나타내며 나아가 표면장력치의 측정이, 신경자극 전달 측정을 위한 Beidler의 이론과 Szyszkowski 방정식을 이용하여 쓴 맛을 기계적으로 측정하는 독립적인 방법을 개발하는데 응용될 수 있음을 시사해 준다.

• International Journal of Oral Biology
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• 제43권3호
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• pp.113-121
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• 2018

Taste is closely related to intake of food. Taste perception is also influenced by type of food ingested, and nutrition and health status. Bitter taste plays an important role in the survival of human and animals to avoid probable toxic and harmful substances. Vertebrate animals recognize bitter taste through type 2 taste receptors (T2Rs). Several T2Rs have been expressed extra-oral such as the gastrointestinal tract, respiratory tract, urogenital tract, brain and immune cells, and parts of their functions are being revealed. This review will discuss physiological roles of T2Rs in relation to innate immunity, secretion and smooth muscle contraction expressed in extra-oral cells and tissues, and we summarize relationships between polymorphisms in T2Rs and general or oral diseases. It is not a coincidence that animals pay much genetic costs for taste and smell during evolution.

• International Journal of Oral Biology
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• 제47권4호
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• pp.49-54
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• 2022

Taste is a basic sensation to get attracted toward nutritious foods or avoid possible harmful substances. The basic taste qualities in humans consist of sweet, bitter, umami, salty, and sour. Basically, sweet and umami tastes make food attractive, whereas bitter and sour tastes make it avoidable. Salty taste comprises basic salty and high salt taste. The basic salty taste is known as amiloride-sensitive salty taste, which is inhibited by amiloride, but the high salt taste is not sensitive to amiloride. Moreover, high salt taste can also cause avoidance behavior in human beings. Sodium, one of the most important cations in the body fluids of vertebrates, controls the volume of total body fluids and is a risk factor for cardiovascular diseases, such as hypertension. The concentration of sodium in body fluids must be under delicate control. A distinction between the salty taste and high salt taste would be a contributing mechanism to control the volume and/or osmolarity of body fluids.

• International Journal of Oral Biology
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• 제37권2호
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• pp.57-62
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• 2012

The nasal cavity encounters various irritants during inhalation such as dust and pathogens. To detect and remove these irritants, it has been postulated that the nasal mucosa epithelium has a specialized sensing system. The oral cavity, on the other hand, is known to have bitter taste receptors (T2Rs) that can detect harmful substances to prevent ingestion. Recently, solitary chemosensory cells expressing T2R subtypes have been found in the respiratory epithelium of rodents. In addition, T2Rs have been identified in the human airway epithelia. However, it is not clear which T2Rs are expressed in the human nasal mucosa epithelium and whether they mediate the removal of foreign materials through increased cilia movement. In our current study, we show that human T2R receptors indeed function also in the nasal mucosa epithelium. Our RT-PCR data indicate that the T2R subtypes (T2R3, T2R4, T2R5, T2R10, T2R13, T2R14, T2R39, T2R43, T2R44, T2R 45, T2R46, T2R47, T2R48, T2R49, and T2R50) are expressed in human nasal mucosa. Furthermore, we have found that T2R receptor activators such as bitter chemicals augments the ciliary beating frequency. Our results thus demonstrate that T2Rs are likely to function in the cleanup of inhaled dust and pathogens by increasing ciliary movement. This would suggest that T2Rs are feasible molecular targets for the development of novel treatment strategies for nasal infection and inflammation.

• KSBB Journal
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• 제17권1호
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• pp.68-72
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• 2002

폐맥주효모로부터 Hop의 고미성분을 추출하는 최적조건은 이산화탄소 유량 50 mL/min, 입자크기 0.35 mm, 시료 20 g, 보조용매(95% 에탄올) 4 mL/min, 총 추출시간 30분, 추출온도 $45^{\circ}C$, 추출 압력 1800 psia로 원 시료와 비교한 결과 Hop의 향기 성분과 고미성분이 거의 제거됨을 알 수 있었다. 또한 보조용매의 효과는 추출물질의 표면적을 크게 하여 침투력을 높여주고 추출물 중의 휘발성이 강한 성분이나 색의 제거에 상당한 효과가 있음을 알 수 있었다(13,14).

• 한국식품저장유통학회지
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• 제11권1호
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• pp.53-56
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• 2004

유자의 부위별 narinign 및 hesperidin의 함량은 과피가 95.54 mg%, 103.99 mg%, 과육이 65.77 mg%, 77.18 mg%, 씨에는 16.49 mg%, 15.88 mg% 순으로 검출되었다 유자에서 고미의 원인 물질인 naringin을 제거하기 위한 naringinase 최적 첨가량을 검토한 결과, naringinase 20 units 첨가구의 경우 90분 경과후 대부분 분해되었다. 표준시약으로 naringin 10 mg%와 hesperidin 5 mg%의 기질에 naringinase 10.0 units와 hesperidinase 2.0 units를 각각 첨가하여 측정한 결과 0.11 mg%와 0.45 mg%로 감소하였고, naringinase 1% 처리구에서 0.54 mg% 와 0.09 mg% 감소하였으며, 탁주용 당화효소 5% 처리구에서는 0.26 mg%, 0.04 mg%로 감소하였다.

• 대한한의학원전학회지
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• 제23권6호
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• pp.27-44
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• 2010

In order to setup the diet guideline of five grains, five meats, five fruits, and five vegetables for the diseases of five organs, I reviewed the their tastes by comparing "Naekyeong" with "Euhakibmun", "Dongeuibogam". 'Ma(麻)' in "Naekyeong" means not a hemp, a ramie or a jute, but a sesame(胡麻;참깨). 'Maik(麥)' in it means both a barley(大麥;보리) and a wheat(小麥;밀). 'Guak(藿)' in it means bean leaves(콩잎), leaves of a red-bean(팥잎) or brown seaweed(海藻;미역). 'Gyu(葵)' in "Euhakibmun Jangbujobun(臟腑條分)" is a miswritten word for 'Welsh onion' caused by similarity of shape of word. Food of a salty taste according to five elemental arrangement in "Naekyeong" is really salty according to "Euhakibmun" and "Dongeuibogam". But a barley(大麥) and a wheat(小麥) of sour taste are bitter, a chicken of sour taste or hot taste is sweet, nonglutinous millet of sour taste is sweet, an apricot of bitter taste is hot, a sesame seed of sweet taste is sour, a nonglutinous rice of hot taste is sweet, and a horsemeat of hot taste is bitter according to them. There are two ways to recommend the food for diseases of five organs. One way is to promote or control the Qi(氣) of five organs according to "Somun(素問)" and "Euhakibmun Jangbujobun", the other way is to build up the Yin(陰血) of five organs according to "Yungchu(靈樞) five tastes(五味)". The two different ways are not contradictory to each other, but complement on the view point of their substances(體) or actions(用).

• Journal of Animal Science and Technology
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• 제63권5호
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• pp.1169-1181
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• 2021

We investigated the comparative physicochemical attributes, oxidative stability, and microbial characteristics of 28 days dry-aged meat in between boneless sirloin (gluteus medius) and bone-in T-bone steaks (infraspinatus) muscles from Korean Native Hanwoo Steer (KNHS). Results reveal that regardless of the muscles, dry-aging increased protein content and water-holding capacity (WHC) (p < 0.05). Meat from infraspinatus-aged muscle led to darker meat with higher pH values than un-aged meat (p < 0.05). However, fat content, CIE a^*, and CIE b^* remained unchanged in both muscles at aging. At aged meat, thiobarbituric acid reactive substances (TBARS) values from bone-in infraspinatus muscle was 2.5-fold higher than boneless gluteus medius muscle (p < 0.05). Dry-aging led to an increase in the contents of total unsaturated fatty acids (UFAs), monounsaturated fatty acids (MUFAs), and UFA/saturated fatty acids (SFA) in both muscles (p < 0.05). Furthermore, gluteus medius aged muscle concentrated with olic acid (C18:1) compared to infraspinatus aged muscle. Irrespective of the muscles, dry-aging enhanced the total free amino acids (FAAs) as well as tasty, and bitter amino acid contents whereas decreased the tasty/bitter amino acids (p < 0.05). Aromatic amino acid, tryptophan that converted to serotonin was 2-fold higher in boneless gluteus medius muscle than bone-in infraspinatus muscle at pre and post aging processes (p < 0.05). Aged Infraspinatus muscle increased total bacteria (p < 0.05) while no salmonella spp. was detected in both muscles. Taken together, our study confirms that 28 days dry-aging profiling the quality characteristics of boneless sirloin (gluteus medius) and bone-in T-bone steaks (infraspinatus) distinctly while gluteus medius aged steak performs better owing to oxidative stability and functional compounds than infraspinatus aged steak.