• 한국지하수토양환경학회:학술대회논문집
• /
• 한국지하수토양환경학회 1999년도 정기총회 및 춘계 공동 학술발표회
• /
• pp.76-77
• /
• 1999

• 한국미생물·생명공학회지
• /
• 제39권2호
• /
• pp.161-167
• /
• 2011

본 연구실에서 확보한 diesel 분해 고효율 균주 Pseudomonas putida KDi 19, kerosene 분해 고효율 균주 P. aeruginosa K14, gasoline 분해 고효율 균주 P. putida G8, BTEX 분해 고효율 균주 P. putida BJ10, P. putida E41의 5개의 고효율 균주를 컬럼 및 반응기에 적용하여 TPH의 생물학적 분해 실험에 적용하였다. 영양염류 및 산소 농도, 균농도 등 최적의 환경인자 도출을 통해 최적의 생물학적 처리 효율을 TPH의 경우, MSM 및 activator I을 주입하여 25일 동안 76.3%의 제거 효율과 제거속도상수 K=0.711를 나타냈으며, diesel의 경우 40일 동안 99.2%의 제거 효율을 보였다. 또한, TPH 오염 토양의 lab-scale bioremediation 실험에서 고효율 균주를 적용한 결과 45일 운전 기간 동안 7,209.9 $mg{\cdot}kg^{-1}$을 825.6 $mg{\cdot}kg^{-1}$까지 88.5% 제거하였다. 본 연구에서 도출된 TPH로 오염된 토양의 bioremediation을 위한 고효율 균주 확보와 최적의 환경 인자 도출은 현재 부족한 생물학적 처리 연구와 물리적 화학적 처리의 문제를 해소하기 위한 기초적 실험 자료로서 기여할 것으로 사료된다.

• Journal of Microbiology and Biotechnology
• /
• 제11권4호
• /
• pp.607-613
• /
• 2001

The biodegradation rates of diesel oil by a selected diesel-degrading bacterium, Pseudomonas stutzeri strain Y2G1, and microbial consortia composed of combinations of 5 selected diesel-degrading bacterial were determined in liquid and soil systems. The diesel degradation rate by strain Y2G1 linearly increased $(R^2=0.98)$ as the diesel concentration increased up to 12%, and a degradation rate as high as 5.64 g/l/day was obtained. The diesel degradation by strain Y2G1 was significantly affected by several environmental factors, and the optimal conditions for pH, temperature, and moisture content were at pH8, $25^{\circ}C$, and 10%, respectively. In the batch soil microcosm tests, inoculation, especially in the form of a consortium, and the addition of nutrients both significantly enhanced the diesel degradation by a factor of 1.5 and 4, respectively. Aeration of the soil columns effectively accelerated the diesel degradation, and the initial degradation rate was obviously stimulated with the addition of inorganic nutrients. Based on these results, it was concluded that the major rate-limiting factors in the tested diesel-contaminated soil were the presence of inorganic nutrients, oxygen, and diesel-degrading microorganisms. To resolve these limiting parameters, bioremediation strategies were specifically designed for the tested soil, and the successful mitigation of the limiting parameters resulted in an enhancement of the bioremediation efficiency by a factor of 11.

• 한국지하수토양환경학회:학술대회논문집
• /
• 한국지하수토양환경학회 2002년도 총회 및 춘계학술발표회
• /
• pp.199-202
• /
• 2002

Many methods have been developed for the remediation of contaminated soil and groundwater. Among those technologies, in-situ bioremediation is most likely to be cost-effective method for petroleum hydrocarbon contamination. But the in-situ bioremediation can require more time to remediate hydrocarbon-contaminated soil and groundwater than other methods. Therefore we intended to save time of in-situ bioremediation using a biological additive to activate indigenous microbes in soil. The additive, 'Inipol EAP 22' stimulates the growth of specific flora, significantly accelerating the speed at which hydrocarbons are biodegraded. And it hans been tested in accordance with protocol approved by the USEPA and is registered on the National Contingency Plan Product Schedule List. In the experiment, three soil samples contaminated with fuel oil were prepared in the same concentration. Inipol EAP 22 was not added to one sample and was added to the other two samples with 5% and 10% of hydrocarbon by weight respectively. And $CO_2$gas derived from bacterial respiration was analyzed in each samples for 15 days. As a result, 145% and 153% of $CO_2$ evolution (microbial respiration) against the sample without 'Inipol EAP 22' occurred in samples with 'Inipol EAP 22' addition of 5% and 10%, respectively

• 한국지하수토양환경학회지:지하수토양환경
• /
• 제19권5호
• /
• pp.9-17
• /
• 2014

This paper reported the use of real-time polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), and the culture-based method in the intrinsic bioremediation study at a petroleum contaminated site. The study showed that phenol hydroxylase gene was detected in groundwater contaminated with benzene, toluene, ethylbenzene, xylene isomers (BTEX) and methyl tert-butyl ether (MTBE). This indicated that intrinsic bioremediation occurred at the site. DGGE analyses revealed that the petroleum-hydrocarbon plume caused the variation in microbial communities. MTBE degraders including Pseudomonas sp. NKNU01, Bacillus sp. NKNU01, Klebsiella sp. NKNU01, Enterobacter sp. NKNU01, and Enterobacter sp. NKNU02 were isolated from the contaminated groundwater using the cultured-based method. Among these five strains, Enterobacter sp. NKNU02 is the most effective stain at degrading MTBE without the addition of pentane. The MTBE biodegradation experiment indicated that the isolated bacteria were affected by propane. Biodegradation of MTBE was decreased but not totally inhibited in the mixtures of BTEX. Enterobacter sp. NKNU02 degraded about 60% of MTBE in the bioreactor study. Tert-butyl alcohol (TBA), acetic acid, 2-propanol, and propenoic acid were detected using gas chromatography/mass spectrometry during MTBE degraded by the rest cells of Enterobacter sp. NKNU02. The effectiveness of bioremediation of MTBE was assessed for potential field-scale application.

• 한국지하수토양환경학회:학술대회논문집
• /
• 한국지하수토양환경학회 2003년도 추계학술발표회
• /
• pp.368-371
• /
• 2003

Accidental release of petroleum products from underground storage tank(USTs) is one of the most common causes of groundwater contamination. BTEX is the major components of fuel oils, which are hazardous substances regulated by many nations. In addition to BTEX, other gasoline consituents such as MTBE(methyl-t-buthyl ether), anphthalene are also toxic to humans. Natual attenuation processes include physic, chemical, and biological trasformation. Aerobic and anaerobic biodegradation are believed to be the major processes that account for both containment of the petroleum-hydrocarbon plum and reduction of the contaminant concentrations. Aerobic bioremediation has been highly effective in the remediation of many fuel releases. However, Bioremediation of aromatic hydrocarbons in groundwater and sediments is ofen limited by the inability to provide sufficient oxygen to the contaminated zones due to the low water solubility of oxygen. Anaerobic processes refer to a variety of biodegradation mechanisms that use nitrate, ferric iron, sulfate, and carbon dioxide as terminal electron accepters. The objectives of this study was to conduct laboratory-scale microcosm studies in assessing enhanced bioremediation potential of BTEX and MTBE under various electron accepters(aerobic, nitrate, ferric iron, sulfate) in contaminated Soil. these results suggest that, presents evidence and a variety pattern of the biological removal of aromatic compounds under enhanced nitrate-, Fe(III)-, sulfate-reducing conditions.

• International Journal of Railway
• /
• 제4권1호
• /
• pp.1-4
• /
• 2011

In this study, the feasibility of using bioremediation to treat lubricant-contaminated soil around railroad turnouts was investigated. Lubricants used during the maintenance of railroad turnouts can drip onto the ground causing soil contamination. In the laboratory experiments, the residual TPH (Total Petroleum Hydrocarbons) concentration in soil gradually decreased after microorganisms degrading the lubricants were added. Generally, the soil around railroad turnouts is covered by a layer of ballasts. In the column experiments that were designed considering field sites, the removal efficiency of TPH was about 11% after 60 days of cultivation time. In the field experiments, microorganisms were added into the soil periodically, and finally the residual TPH concentrations were reduced to less than 1,700 mg/kg-soil on average. These results indicate that the lubricant in the contaminated soil around railroad turnouts could be efficiently removed through bioremediation method.

• 한국지하수토양환경학회지:지하수토양환경
• /
• 제7권1호
• /
• pp.15-24
• /
• 2002

현재 원위치 bioremediation을 목적으로 EK (electrokinetlc)기법에 의한 영양분과 TEAs (Terminal electron accepters) 공급이 연구되고 있으나, 아직까지 다양한 토질에서의 EK 주입특성 연구는 미비하다. 따라서 본 연구에서는 카올린과 모래시료에 대하여 영양분인 암모늄이온과 TEAs로서 황산염이온의 EK주입실험을 수행하였다. EK주입에 의한 암모늄이온의 경우 카올린에서는 주입부(양극)에서 높은 농도분포를 보이며, 특별히 모래의 경우는 음극에서 더욱 높은 농도분포를 보인다. 황산염이온의 주입분포는 두 시료에서 모두 균일한 주입분포를 보이지만 두 시료에서의 농도차는 크게 달랐다. 따라서 원위치 bioremediation에 EK 주입기법을 이용할 경우 토질에 따른 주입특성의 평가가 가장 중요하게 고려되어야 한다.

• Environmental Engineering Research
• /
• 제24권3호
• /
• pp.495-500
• /
• 2019

Bioremediation of bacterial algae is one of wastewater treatment by utilizing symbiosis of bacterial algae, which is relatively inexpensive and safe for the environment. The aims of this research were: (1) to investigate initial characteristic of boezem water of Kalidami Surabaya, (2) to investigate the effect of potassium (K) element and carbon source addition toward the reduction of $NH_3-N$ content and organic matter in $KMnO_4$ of boezem water. The research conducted in a laboratory in batches without adding aeration. The initial stage of this research was conducting alga culture until it was obtained chlorophyll-a algae concentration of $3.5{\pm}0.5mg/L$. The best result of range finding test was a comparison of boezem water volume with algal which were about 25%:75%. The research conducted in duplo over 18 d. The result of the research can be concluded that boezem water of Kalidami Surabaya for the parameter of pH, temperature, $NH_3-N$, dissolved oxygen, chemical oxygen demand, biological oxygen demand, and number of $KMnO_4$ show that it enables to do bioremediation of bacterial algae. Decrease efficiency occurred in a reactor with the addition of element K 3% and source C. $NH_3-N$ and $KMnO_4$ final content 0.164 mg/L and 30 mg/L, respectively.

• Journal of Microbiology and Biotechnology
• /
• 제24권9호
• /
• pp.1269-1279
• /
• 2014

Xanthomonas oryzae pv. oryzae (Xoo) strains are plant pathogenic bacteria that can cause serious blight of rice, and their virulence towards plant host is complex, making it difficult to be elucidated. Caenorhabditis elegans has been used as a powerful model organism to simplify the host and pathogen system. However, whether the C. elegans is feasible for studying plant pathogens such as Xoo has not been explored. In the present work, we report that Xoo strains PXO99 and JXOIII reduce the lifespan of worms not through acute toxicity, but in an infectious manner; pathogens proliferate and persist in the intestinal lumen to cause marked anterior intestine distension. In addition, Xoo triggers (i) the p38 MAPK signal pathway to upregulate its downstream C17H12.8 expression, and (ii) the DAF-2/DAF-16 pathway to upregulate its downstream gene expressions of mtl-1 and sod-3 under the condition of daf-2 mutation. Our findings suggest that C. elegans can be used as a model to evaluate the virulence of Xoo phytopathogens to host.