• Journal of Microbiology and Biotechnology
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• 제28권11호
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• pp.1865-1875
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• 2018

Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of Rhizopus K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of Rhizopus K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, ${\mu}$ of mycelium reached to $0.15h^{-1}$ after 45 h of culture in a thermostatic water bath incubator at $30^{\circ}C$. Under a low-magnification microscope, mycelial cells appeared uniform, bulky with numerous branches, and were not easily ruptured. The generated glucoamylase activity reached to 55 U/g (dry basis). This study has good utilization value for glucoamylase production by Rhizopus in SSF.

• 한국농공학회:학술대회논문집
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• 한국농공학회 2002년도 학술발표회 발표논문집
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• pp.413-416
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• 2002

Global warming is one of the major international concerns and renewable energy development and utilization are getting more attention recently. One of the competitive renewable energy alternatives is biomass. This paper describes the major concepts of gasification and fuel gas applications.

• Journal of Microbiology and Biotechnology
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• 제22권11호
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• pp.1532-1539
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• 2012

The ${\alpha}$-galactosidase-coding gene agaAJB13 was cloned from Sphingomonas sp. JB13 showing 16S rDNA (1,343 bp) identities of ${\leq}97.2%$ with other identified Sphingomonas strains. agaAJB13 (2,217 bp; 64.9% GC content) encodes a 738-residue polypeptide (AgaAJB13) with a calculated mass of 82.3 kDa. AgaAJB13 showed the highest identity of 61.4% with the putative glycosyl hydrolase family 36 ${\alpha}$-galactosidase from Granulicella mallensis MP5ACTX8 (EFI56085). AgaAJB13 also showed <37% identities with reported protease-resistant or Sphingomonas ${\alpha}$-galactosidases. A sequence analysis revealed different catalytic motifs between reported Sphingomonas ${\alpha}$-galactosidases (KXD and RXXXD) and AgaAJB13 (KWD and SDXXDXXXR). Recombinant AgaAJB13 (rAgaAJB13) was expressed in Escherichia coli BL21 (DE3). The purified rAgaAJB13 was characterized using p-nitrophenyl-${\alpha}$-D-galactopyranoside as the substrate and showed an apparent optimum at pH 5.0 and $60^{\circ}C$ and strong resistance to trypsin and proteinase K digestion. Compared with reported proteaseresistant ${\alpha}$-galactosidases showing thermolability at $50^{\circ}C$ or $60^{\circ}C$ and specific activities of <71 U/mg with or without protease treatments, rAgaAJB13 exhibited a better thermal stability (half-life of >60 min at $60^{\circ}C$) and higher specific activities (225.0-256.5 U/mg). These sequence and enzymatic properties suggest AgaAJB13 is the first identified and characterized Sphingomonas ${\alpha}$-galactosidase, and shows novel protease resistance with a potential value for basic research and industrial applications.

• Journal of Forest and Environmental Science
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• 제25권2호
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• pp.131-138
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• 2009

Recently, various efforts for the extended utilization of woody biomass has been attempted due to the fact that global warming, energy and environmental problems are urgent ones to be solved. Development of new energy sources at our national security level is desperately needed as we depend on almost all of energies supplied from other countries, let alone the economic crisis caused by oil price hike. Woody biomass can be converted to energy by means of thermochemical, biological, or direct combustion processes. Many processes are available for producing bioenergy, such as bioethanol, wood pellet, wood chip, combined heat, and power system. Political support and R&D investment should be provided that can boost the utilization of the wood biomass, the eco-environment, and recyclable and alternative energy resources for national power security. In addition, a long-term strategy that can utilize unused and low efficient woody biomass resources, and systematically collect and manage them in a national level should be set up. Even though the possibility in total exchange of fossil oil with woody biomass is quite low, technology developments of woody biomass for the solution to global warming and environmental problem through its commercialization are expected to grow steadily.

• 한국에너지공학회:학술대회논문집
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• 한국태양에너지학회, 한국에너지공학회 1993년도 춘계 공동학술발표회 초록집
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• pp.20-32
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• 1993

Although the importance of agricultural sector decreased over the last several decades because of the economic growth in Korea led by the development of manufacturing sector, the biomass energy resources such as urban wastes, industrial wastes including agricultural residues emerged recently as a major target of development mainly because of environmental issues. (omitted)

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2007년도 추계학술대회 논문집
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• pp.658-661
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• 2007

It was investigated on this paper that the status of biomass energy utilization in the registered CDM projects. The ratio of the case including the energy recovery among the registered biomass CDM projects is just about 57%. Direct thermal conversion technology is the most commonly applied for biomass energy recovery projects. Through the comparative study with world renewable energy outlook, the contribution of CDM to biomass energy supply in the world is not considerable yet.

• 신재생에너지
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• 제18권3호
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• pp.10-22
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• 2022

Forest biomass energy is based on scientific evidence in response to carbon neutrality and the climate crisis, international consensus, and environmental-geographic characteristics of each nation. In this study, the authors aimed to analyze macroscopic forest biomass energy policies for ten major countries. They categorized them into six detailed categories (Sustainable utilization, Cascading Uutilization, Replacement of fossil fuel/Carbon intensive products, Utilization of forest by-products/residues as the source of energy, Contribution to carbon-neutral/climate change, and Biomass combined with CCS/CCUS ). In addition, the surveyed nations have developed a policy consensus on the active use of forest biomass with sustainable forest management except for the cascading utilization category. Furthermore, the authors evaluated the mid to long-term plans of the Korean government for improvements in the policy and legal aspects. As a result, the authors derived four major directions that South Korea should approach strategically in the future (1) secure financial resources for sustainable forest management and stimulating investment in the timber industry, (2) promote unified policies to establish a bio-economy, (3) enhancement of the forest biomass energy system, and (4) reorganization and promotion of strategy centered on the opinions of field experts in internal and external instability.

• 한국산림과학회지
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• 제104권2호
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• pp.230-238
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• 2015

현재 우리나라의 주요 임업기계에 의한 임목수확작업시스템에서 벌목 및 조재, 집재, 소운재, 파쇄 등의 목재생산 및 파쇄작업에 대한 작업비용을 분석하여, 산림바이오매스 이용을 위한 효율적인 임목수확작업시스템을 구축하기 위한 기초자료를 제공하고자 수행하였다. 임목수확작업시스템의 작업비용을 분석한 결과, 체인톱에 의한 벌목 및 조재와 타워야더에 의한 집재, 임내작업차에 의한 소운재, 트럭에 의한 운재작업으로 이루어진 임목수확작업시스템 D가 63,482원/$m^3$으로 가장 적은 비용이 소요되는 것으로 나타났다. 또한, 산림바이오매스 이용을 위한 작업시스템의 작업비용을 분석한 결과, 소형 목재파쇄기에 의한 현장파쇄, 임내작업차에 의한 칩 소운반, 트럭에 의한 칩 운반작업의 작업시스템 E가 90,770원/ton으로 가장 적은 비용이 산출되었다. 따라서, 이들의 결과가 임목수확작업 및 산림바이오매스 이용을 위한 효율적인 작업시스템이라고 판단된다.

• Journal of Microbiology and Biotechnology
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• 제23권6호
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• pp.794-801
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• 2013

This paper reports the production and characterization of crude xylanase from the newly isolated Humicola sp. Ly01. The highest (41.8 U/ml) production of the crude xylanase was obtained under the optimized conditions (w/v): 0.5% wheat bran, 0.2% $KH_2PO_4$, and 0.5% peptone; initial pH 7.0; incubation time 72 h; $30^{\circ}C$; and 150 rpm. A considerable amount of the crude xylanase was induced using hulless barley bran or soybean meal as the carbon source, but a small amount of the enzyme was produced when supplementary urea was used as the nitrogen source to wheat bran. The crude xylanase showed apparent optimal cellulase-free xylanase activity at $60^{\circ}C$ and pH 6.0, more than 71.8% of the maximum xylanase activity in 3.0-30.0% (v/v) ethanol and more than 82.3% of the initial xylanase activity after incubation in 3.0-30.0% (v/v) ethanol at $30^{\circ}C$ for 2 h. The crude xylanase was moderately resistant to both acid and neutral protease digestion, and released 7.9 and 10.9 ${\mu}mol/ml$ reducing sugar from xylan in the simulated gastric and intestinal fluids, respectively. The xylooligosaccharides were the main products of the hydrolysis of xylan by the crude xylanase. These properties suggested the potential of the crude enzyme for being applied in the animal feed industry, xylooligosaccharides production, and high-alcohol conditions such as ethanol production and brewing.

• 한국농공학회논문집
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• 제46권3호
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• pp.85-92
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• 2004

Biomass is considered to be a major potential fuel and renewable resource for the future. In fact, there is high potential to produce significant amount of energy from biomass around the world. In spite of the potential, there are a few efforts in biomass utilization in this nation. In this study, elemental biomass data was obtained with respect to the amount and calorific values of agricultural residues. Rice straw and husks were not included in the evaluation due to their demand from alternative uses such as livestock feedings, bedding materials, and so forth. Dry basis high calorific values are about 4,500 kcal/kg for all the agricultural residues investigated, similar to literature data. Energy densities or unit area energy value, from pepper and sesame were significant and comparable to those of woody biomass. These elemental data for biomass resources will Provide the background of Planning and development of biomass energy Program, which is getting more feasible along with advances in energy conversion technologies such as micro gas turbines.