• 한국자원식물학회지
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• 제21권3호
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• pp.222-225
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• 2008

Chemical composition and enzymatic saccharification characteristics of hemp woody core were investigated by their chemical composition analysis and enzymatic saccharification with commercially available cellulases (Celluclast 1.5L and Novozym 342). Hemp woody core have higher xylan and lower lignin contents than its bast fiber. Based on hemicelluloses and lignin composition, hemp woody core is similar with hardwood biomass. However, cellulose was more easily converted to glucose than xylan to xylose and this trend was confirmed both hemp woody core and yellow poplar. Hemp woody core biomass shows higher saccharification than yellow poplar (hardwood biomass) based on cellulose and xylan hydrolysis. With easier enzymatic saccharification in cellulose and xylan, and similar chemical composition, hemp woody core have better biorefinery feedstock characteristics than hardwood biomass.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2008년도 추계학술대회 논문집
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• pp.74-76
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• 2008

Autohydrolysis at different temperature levels was applied as industrial hemp pretreatment technique for glucose generation. Main structural components removed by autohydrolysis was xylan, which is more sensitive in acidic hydrolysis condition than cellulose or lignin. Higher temperature reaction conditions promoted more biomass components (xylan) removal than lower temperature, which led to better respond to enzymatic saccharification of residual biomass after autohydrolysis. With $185^{\circ}C$ and 60 min, saccharification degree was 53.0% of cellulose in hemp woody core biomass.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2008년도 춘계학술대회 논문집
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• pp.225-228
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• 2008

This study shows that lignocellulosic biomass saccharification work has been carried out with rice-straw by the extracellular enzyme from KMU001, and the enzymes produced in 5%(w/v) wood biomass were characterized by protein and various enzyme activity measurements. Several cellulases such as Endoglucanase(EG), $\beta$-D-1,4-Glucosidase(BGL), Cellobiohydrolase(CBH), and $\beta$-D-1,4-Xylanase (BXL) were detected. Saccharification of rice-straw by the enzyme yielded about 233mg/g of glucose after 48hrs.

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.297-302
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• 1999

Simultaneous saccharification and fermentation (SSF) experiments were carried out with a lignocellulosic biomass. The effects of temperature on enzymatic saccharification and the ethanol fermentation were also investigated. The batch SSF process gave a final ethanol concentration of 10.44 g/l and equivalent glucose yield of 0.55 g/g, which was increased by 67％ or higher over the saccharification at 42℃. The optimal operating condition was found to vary in several parameters, such as the transmembrane pressure, permeation rate, and separation coefficient, related to the SSF combined with membrane system (semi-batch system). When the fermentation was operated in a semi-batch mode, the efficiency of the enzymes and yeast lasted three times longer than in a batch mode.

• 펄프종이기술
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• 제42권5호
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• pp.15-23
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• 2010

Bio-ethanol is the most potential next generation automotive fuel for reducing both consumption of crude oil and environmental pollution from renewable resources such as wood, forest residuals, agricultural leftovers and urban wastes. Lignocellulosic based materials can be broken down into individual sugars. Therefore, saccharification is one of the important steps for producing sugars, such as 6-C glucose, galactose, mannose and 5-C xylose, mannose and rhamnose. These sugars can be further broken down and fermented into ethanol. The main objective of this research is to study the feasibility and optimize saccharification and fermentation process for the conversion of lignocellulosic biomass to low cost bioethanol.

• 에너지공학
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• 제24권3호
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• pp.1-5
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• 2015

본 연구는 바이오에탄올을 생산하고자 목질계 바이오매스의 효소당화에서의 반탄화의 영향을 비교분석하였다. 전처리로서, 목질계 바이오매스의 반탄화는 무산소 조건에서 $250{\sim}350^{\circ}C$의 온도로 시행되었다. 또한 비이온성 계면활성제인 Tween-80을 첨가하여 반탄화로 인한 소수성변환에 대처하여 당화효율을 높이기 위한 실험을 진행하였다. 그 결과, 반탄화 전처리한 바이오매스를 효소당화한 후 글루코즈 생산량이 전처리하지 않은 바이오매스의 글루코즈 생산량보다 높았다. 그리고 Tween-80의 첨가하여 효소당화하였을 때 당 전환율이 더 높았다. 이로 인해 반탄화를 목질계 바이오매스의 전처리로 적용할 수 있으며 Tween-80을 첨가하였을 때 효소당화에 영향이 있다는 것을 알 수 있었다.

• Mycobiology
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• 제30권3호
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• pp.166-169
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• 2002

Protein productivity by the cellulolytic fungi, Trichoderma viride(MTCC 800), Chaetomium globosum and Aspergillus terreus was compared in co-culture and mixed culture fermentations of cashewnut bran. Co-cultures were more effective in substrate saccharification, which ranged between $85{\sim}88%$ compared to the $62{\sim}67%$ saccharification shown by the monocultures. Maximum saccharification was induced by T. viride and C. globosum co-culture resulting in the highest 34% release of reducing sugars. The maximum 16.4% biomass protein and the highest protein productivity(0.58%) were shown by T. viride and A. terreus co-culture. A. terreus performed better in co-culture in the presence of T. viride rather than with C. globosum. Among the cellulolytic enzymes, FPase(Filter Paper Cellulase) activity was significantly higher in all the co-cultures and in the mixed culture than in their respective monocultures. Mixed culture fermentation involving all the three fungi was not effective in increasing the per cent saccharification or the biomass protein content over the co-cultures.

• Journal of Microbiology and Biotechnology
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• 제29권11호
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• pp.1760-1768
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• 2019

The use of lignocellulosic biomass such as rice straw can help subsidize the cost of producing value-added chemicals. However, inhibitory compounds, such as phenolics, produced during the pre-treatment of biomass, hamper the saccharification process. Laccase and electrochemical stimuli are both well known to reduce phenolic compounds. Therefore, in this study, we implemented a bioelectrochemical detoxification system (BEDS), a consolidated electrochemical and enzymatic process involving laccase, to enhance the detoxification of phenolics, and thus achieve a higher saccharification efficiency. Saccharification of pretreated rice straw using BEDS at 1.5 V showed 90% phenolic reduction (Ph_r), thereby resulting in a maximum saccharification yield of 85%. In addition, the specific power consumption when using BEDS (2.2 W/Kg Ph_r) was noted to be 24% lower than by the electrochemical process alone (2.89 W/kg Ph_r). To the best of our knowledge, this is the first study to implement BEDS for reduction of phenolic compounds in pretreated biomass.

• Journal of Forest and Environmental Science
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• 제27권3호
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• pp.143-149
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• 2011

The possibility of employing biomass of Salix viminalis cv. Q683 as a resource of bio-energy was evaluated. The chemical analysis of S. viminalis cv. Q683 leaf biomass showed components such as, extractives (2.57%), lignin (39.06%), hemicellulose (21.61%), and cellulose (37.83%), whereas, its stem was composed of extractives (1.67%), lignin (23.54%), hemicellulose (33.64%), and cellulose (42.03%). The biomass of S. viminalis cv. Q683 was saccharified using two enzymes celluclast and viscozyme. The saccharification of S. viminalis cv. Q683 biomass was influenced by enzymes and their strengths. The optimal enzyme combination was found to be celluclast (59 FPU/g substrate) and viscozyme (24 FBG/g substrate). On saccharification the glucose from leaf and stem biomass was 7.5g/L and 11.7g/L, respectively after 72 hr of enzyme treatment. The biomass and enzyme-treated biomass served as the feedstock for ethanol production by fermentation. The ethanol production from stem and leaf biomass was 5.8 g/L and 2.2 g/L respectively, while the fermentation of the enzymatic hydrolysates yielded 5 g/L to 8 g/L bioethanol in 72 hours.

• 공업화학
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• 제26권4호
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• pp.511-514
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• 2015

차세대 바이오에탄올로 주목받고 있는 목질계 바이오매스를 이용한 셀룰로오스 에탄올 생산과정은 셀룰로오스를 단당류로 분해하는 전처리과정이 가장 중요한 역할을 한다. 본 연구에서는 산가수분해와 효소당화과정을 이용하여 볏짚, 톱밥, 복사지, 신문지 등과 같은 목질계 바이오매스로부터 셀룰로오스에탄올을 제조하였다. 전처리과정으로 10~30 wt% 황산을 이용한 산가수분해($100^{\circ}C$, 1 h), celluclast ($55^{\circ}C$, pH = 5.0), AMG ($60^{\circ}C$, pH = 4.5), spirizyme ($60^{\circ}C$, pH = 4.2)을 이용한 효소당화과정(30 min), 산가수분해 후 효소당화과정을 비교하였다. 전처리과정의 수율은 hybrid 과정 > 산가수분해 > 효소당화 순으로 셀룰로오스 에탄올로의 전환이 잘 이루어지는 것으로 나타났으며, 최적 발효시간은 2일이었다. 또한 20 wt% 황산을 이용한 산가수분해 후 celluclast를 이용하여 효소당화를 수행할 경우 톱밥 > 볏짚 > 복사지 > 신문지 순으로 셀룰로오스 에탄올 전환특성이 높게 나타났다.