• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.120-125
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• 2005

Continuous degradation of forest in both quality and quantity threatens wood security in the future. Thus in the future, most wood and pulp will be expected to be produced from plantation forests. We attempt to produce superior trees suitable for such plantations with maximum productivity in limited land area. Tree productivity could be enhanced either by promoting growth and wood quality or by reducing loss caused by abiotic and biotic stresses. Genetic transformation techniques may offer ways to improve the productivity by enabling trees to tolerate the stresses or to covert limited resources into big biomass. With the availability of information on various functional genes and gene transfer techniques, it should be possible to develop such trees. In this presentation, our work to produce such trees at Korea Forest Research Institute is briefly introduced.

• Microbiology and Biotechnology Letters
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• v.22 no.3
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• pp.277-282
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• 1994

In batch cultures of Lactobacillus delbrueckii, cell growth and lactic acid production were affected by two main factors, inhibition by lactic acid and limitation by nutritional components. In order to increase th productivity significantly, a continuous stirred tank reactor with cell recycle was employed. A cell desnity of 145g dry weight/l and a volumetric productivity of 73 g/l$\cdot $h were obtained with an effluent concentration of 85 g/l lactic acid. The productivity achieved by this system was 23-fold higher than those obtained by the corresponding batch cultivations. Once the lactic acid concentration reached the steady steady state, lowering the yeast extract concentration caused the reduction of the lactic acid concentration without affection the biomass concentration. Finally, the formation of D-lactate was investgated. During the various cultures, a small amount of D-lactate always formed, even thought a majority of lactate was L-isomer, It was supposed that the relative amount of the D-lactate was affected by glucose limitation, and there seems to exist a certain relationship between the concentration of D-lactate and acetate.

• The Korean Journal of Ecology
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• v.24 no.6
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• pp.335-339
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• 2001

The dynamic model was developed to simulate the photosynthetic rate of Phragmites communis stands in coastal ecosystem. The model was composed of the compartments of both climatic and biological variables. The former were photosynthetic photon flux density(PPFD), daily maximum- and minimum-temperature. The latter were combinations of the specific physiological responses of plant organs with the biomass of each organs. The PPFD and air temperature were calculated and using those values, gas exchange rate of each plant organ was calculated at every hour. The carbon budget was constructed using the modelled predictions. Analysis of annual productivity and fluxes showed that yearly gross population productivity, yearly population respiration and yearly net population productivity were 33.4, 21.3 and 12.1 $CO_2ton{\cdot}ha^{-2}{\cdot}yr^{-1}$, respectively. The final result was tested over two stands, produced promising predictions with regards to the levels of production attained. The model can be used to determine production potential under given climatic conditions and could even be applied to plant canopies with analogous biological characteristics.

• Journal of Korean Society of Forest Science
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• v.103 no.4
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• pp.583-592
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• 2014

This study was carried out to investigate the operational time and productivity of logging operation by chain saw, yarder attached on tractor, tower-yarder, mini-truck, mini-forwarder, and chipping operations by mini-chipper, large-chipper in order to develop the efficient logging operation system for utilization of forest-biomass. As a result, the average felling and bucking time using chain saw at the site 1 and 2 was observed to be 182.7 sec/cycle and 518.5 sec/cycle respectively. The average yarding time was 202.5 sec/cycle using yarder attached on tractor and 295.1 sec/cycle using tower-yarder. The average forwarding time was 2,073 sec/cycle using mini-truck and 2,248.4 sec/cycle using mini-forwarder. The operational time of felling and bucking using chain-saw can be delayed according to the direction of fallen trees. The selection of felling direction is very important to yarding operation because the direction between width-yarding and felling are interrelated. Productivity can be improved through educating and training operators in the yarding operations. Mini-forwarder is needed to use because of higher productivity and lower cost than mini-truck. The operational productivity of felling and bucking by chain saw was $66.96m^3/man{\cdot}day$ and $43.86m^3/man{\cdot}day$ at site 1 and 2 respectively. The yarding productivity was $5.68m^3/man{\cdot}day$ by yarder attached on tractor, $10.74m^3/man{\cdot}day$ by tower-yarder. The forwarding productivity was $21.29m^3/man{\cdot}day$ by mini-truck, $28.57m^3/man{\cdot}day$ by mini-forwarder. The chipping productivity was $4.42m^3/man{\cdot}day$ by mini-chipper, $21.87m^3/man{\cdot}day$ by large-chipper.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.1
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• pp.1-7
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• 2011

Application of biochar to soils is proposed as a significant, long-term, sink for atmospheric carbon dioxide in terrestrial ecosystems. In addition to reducing emissions and increasing the sequestration of carbon, production of biochar and its application to soils will contribute improve soil quality and crop productivity. Objectives were i) to evaluate biochar productivity from crop residues using a low-cost field scale mobile pyrolyzer and ii) to evaluate characteristics of feedstocks and biochars from locally collected crop residues. Pyrolysis experiments were performed in a reactor operated at $400-500^{\circ}C$ for 3-4 hours using biomass samples of post-harvest residues of corn (Zea mays L.), cotton (Gossypium spp.), rice (Oryza sativa L.), sorghum (Sorghum bicolor L.) and wheat (Triticum aestivum L.). Feedstocks differed, but average conversion to biochar was 23%. Carbon content of biomass feedstock and biochar samples were 445 g $kg^{-1}$ and 597 g $kg^{-1}$, respectively. Total carbon content of biochar samples was 34% higher than its feedstock samples. Significant increases were found in P, K, Ca, Mg, and micro-nutrients contents between feedstock and biochar samples. Biochar from corn stems and rice hulls can sequester by 60% and 49% of the initial carbon input into biochar respectively when biochar is incorporated into the soils. Pyrolysis conversion of corn and rice residues sequestered significant amounts of carbon as biochar which has further environmental and production benefits when applied to soils. Field experiment with crop residue biochar will be investigated the stability of biochars to show long-term carbon sequestration and environmental influences to the cropping systems.

• KSBB Journal
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• v.10 no.4
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• pp.401-405
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• 1995

An open pond type photobioreactor for mass cultivation of S. platensis was designed and the growth parameters from different cultivation processes were compared. 0.30(1/day) of specific growth rate and 1.69(g/$\ell$) of maximum cell density were obtained from batch cultivation. In fed-batch cultivation, specific growth rate and maximum cell density were estimated as 0.22(1/day) and 1.75(g/$\ell$), respectively. Maximum biomass productively from continuous cultivation was obtained as 0.44 (g/$\ell$/day). It proves that an outdoors-mass cultivation of S. platensis considering optimal environmental condition is economically feasible. In addition, the biomass productivity was studied in two different mixing systems such as agitation and air sparging methods. The biomass productivity by an agitation method was better than that in an air sparging method.

• Journal of Ecology and Environment
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• v.29 no.3
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• pp.237-245
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• 2006

Changes of the biomass, net primary productivity and P/B ratio during abandoned field succession after shifting cultivation were investigated in Pyoungchang, Gangwon-Do, Korea. Aboveground standing biomass of herb species showed the maximum value (3.8 ton/ha) in the 5 year old-field, and decreased thereafter. Litter dry weight was depicted as a parabola form showing the gradual increment during the first 50 years and slight decrease thereafter. Basal area tended to increase logarithmically during abandoned field succession. Tree density showed the gradual increment during the first 15 years and decreased thereafter by the self-thinning process. In the later successional stage (80 years old-field), the shoot density distribution of the tree species by DBH class showed the reverse J shaped curve and Quercus mongolica dominated. Total standing biomass increased slowly in the earlier successional stages and later successional stages, and increased rapidly during the mid-successional stages ($10{\sim}50$ years old-field). Total standing biomass in the 5, 10, 20, 50 and 80 years old-fields were estimated 5, 14, 75, 251 and 373 ton/ha, respectively. Annual net primary productivity were depicted as a parabola form showing the gradual increment during the first 35 years and declined thereafter. The increment rates of the annual net primary productivity in the earlier successional stages showed the higher value than mid-successional stages. The annual net primary productivities in the 5, 10, 20, 35, 50 and 80 years old-field were estimated 8.6, 9.3, 12.9, 15.1, 13.7 and 3.6 ton/ha/yr, respectively. The estimated P/B ratio tended to decrease exponentially during abandoned field succession. The estimated P/B ratio in the 5, 10, 20, 50 and 80 years old-field were 0.60, 0.39, 0.19, 0.06 and 0.01, respectively. These results were fairly in accordance with the bioenergetics model during the forest succession projected by Odum(1969).

• Korean Journal of Environment and Ecology
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• v.30 no.6
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• pp.1022-1031
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• 2016

Relationships of standing biomass with biodiversity and structural diversity were examined in the Quercus mongolica-dominated forest in Mt. Jeombong, Gangwon-do. We examined the standing biomass of the Q. mongolia community ($311.1ton{\cdot}ha^{-1}$) from 2004 to 2013, and the observed major species were Q. mongoilca, Carpinus cordata, Tilia amurensis whose standing biomasses were $206.3ton{\cdot}ha^{-1}$ (66.3%), $36.9ton{\cdot}ha^{-1}$ (11.9%), and $30.6ton{\cdot}ha^{-1}$ (9.8%), respectively. Although the number of Q. mongolica individuals was very small compared with total density, the reason that Q. mongolica showed the most biomass than other species is due to greater average diameter at breast height (DBH) and the higher number of $DBH{\geq}50cm$ individuals. We calculated the range of Shannon index (H') and Shannon evenness (J') in the Q. mongolica community, and they were gradually increased in time, showing 2.015~2.166, 0.673~0.736, respectively. Their H' and J' showed positive linear relationships with their standing biomass. This indicates that the spatial distribution of the standing biomass in Q. mongoilca community becomes more homogeneous with time and this homogenization appears in various species in the community. In addition, we estimated biomass-species index (BS) and abundance-biomass-speciesdiversity (ABS) and they also showed gradual increase in time, ranging from 3.746 to 3.811 and from 4.781 to 5.028, respectively. Their indices showed positive linear relationships with the standing biomass. This can be explained from the observations of variations in standing biomass with tree diameters as the differences in the average standing biomass in the community have reduced gradually in time. Moreover, it is expected that increase in the structure diversity of the Q. mongoilca community enhances the efficiency in carbon sequestration and productivity, so the community can be developed to a more sustainable ecosystem with more abundant resources. Thus, applications of uneven-aged plantations with considerations of local ecological properties can be a very efficient reforestation method to ensure stable support of biodiversity and productivity.

• Journal of Microbiology and Biotechnology
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• v.25 no.9
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• pp.1547-1554
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• 2015

The potential of microalgae biofuel has not been realized because of the low productivity and high costs associated with the current cultivation systems. In this study, a new low-cost and transparent attachment material was tested for cultivation of a filamentous algal strain, Stigeoclonium sp., isolated from wastewater. Initially, the different materials tested for Stigeoclonium cultivation in untreated wastewater were nylon mesh, polyethylene mesh, polypropylene bundle (PB), polycarbonate plate, and viscose rayon. Among the materials tested, PB led to a firm attachment, high biomass (53.22 g/m², dry cell weight), and total lipid yield (5.8 g/m²) with no perceivable change in FAME profile. The Stigeoclonium-dominated biofilm consisted of bacteria and extracellular polysaccharide, which helped in biofilm formation and for effective wastewater treatment (viz., removal efficiency of total nitrogen and total phosphorus corresponded to ~38% and ~90%, respectively). PB also demonstrated high yields under multilayered cultivation in a single reactor treating wastewater. Hence, this system has several advantages over traditional suspended and attached systems, with possibility of increasing areal productivity three times using Stigeoclonium sp. Therefore, multilayered attached growth algal cultivation systems seem to be the future cultivation model for large-scale biodiesel production and wastewater treatment.

• Journal of Microbiology and Biotechnology
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• v.30 no.1
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• pp.136-145
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• 2020

Chlorella sp. HS2, which previously showed excellent performance in phototrophic cultivation and has tolerance for wide ranges of salinity, pH, and temperature, was cultivated heterotrophically. However, this conventional medium has been newly optimized based on a composition analysis using elemental analysis and ICP-OES. In addition, in order to maintain a favorable dissolved oxygen level, stepwise elevation of revolutions per minute was adopted. These optimizations led to 40 and 13% increases in the biomass and lipid productivity, respectively (7.0 and 2.25 g l^-1d^-1 each). To increase the lipid content even further, 12 h heat shock at 50℃ was applied and this enhanced the biomass and lipid productivity up to 4 and 17% respectively (7.3 and 2.64 g l^-1d^-1, each) relative to the optimized conditions above, and the values were 17 and 14% higher than ordinary lipid-accumulating N-limitation (6.2 and 2.31 g l^-1d^-1). On this basis, heat shock was successfully adopted in novel Chlorella sp. HS2 cultivation as a lipid inducer for the first time. Considering its fast and cost-effective characteristics, heat shock will enhance the overall microalgal biofuel production process.