• 한국산림과학회지
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• 제85권3호
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• pp.443-452
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• 1996

충청북도 충주지역에서 평균수령 67, 62년생 신갈나무와 굴참나무 천연림을 대상으로 표준지를 선정하고 각 표준지에서 각각 10본의 표본목을 선정, 벌채하여 지상부의 현존량과 물질 생산성을 조사 분석하였다. 충주지역의 현존량 및 물질 생산성의 추정에 상대생장식을 사용하여 추정하였는데, 추정 방정식 모형으로는 $Wt=aD^bH^c$가 $Wt=a(D^2H)^b$, $Wt=aD^b$ 보다 적합하였고, 두 수종 모두 성분별 단목 현존량의 표본값과 추정치를 T-검정하여 본 결과 유의차는 없었다. 임분의 현존량은 신갈나무 임분의 지상부 총량이 130.6 t/ha였고, 굴참나무 임분이 137.4 t/ha였다. 부위별로는 신갈나무 임분의 경우, 엽 5.1 t/ha(3.9%), 죽은가지 3.5 t/ha(2.7%), 생지부 29.7 t/ha(23.0%), 수피 16.2 t/ha(l2.5%), 그리고 수간목부 74.9 t/ha(58.0%)였다. 굴참나무 임분은 엽 3.8 t/ha(2.9%), 죽은가지 2.9 t/ha(2.2%), 생지부 24.3 t/ha(l8.4), 수피 20.4 t/ha(15.5%), 그리고 수간목부 80.4 t/ha(61.0%)였다. 두 임분의 1년간 생산할 수 있는 지상부 총량은 각각 신갈나무 임분이 10.0 t/ha/yr, 굴참나무 임분이 8.6 t/ha/yr였다. 본 참나무 천연림은 장령림이지만 온대 활엽수림의 순생산량(5~10 t/ha/yr)과 거의 유사한 양을 보였다.

• 한국산림과학회지
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• 제88권2호
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• pp.266-272
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• 1999

경기도 광릉의 중부임업시험장내 31년생 리기다소나무와 낙엽송조림지를 대상으로 임분별 3개의 $20{\times}10m$ 조사구를 선정하고 각 조사구로부터 1본씩 표본목을 선정한 후 벌도하여 지상부 현존량을 측정하고 각 부위별 양분분포를 조사하였다. 조사된 임분의 지상부 현존량은 리기다소나무의 경우 170.2ton/ha, 낙엽송은 87.2ton/ha로서 리기다소나무 임분이 낙엽송 임분에 비해 높게 나타났으며, 두 임분의 현존량의 차이는 임분 밀도의 차가 원인인 것으로 나타났다. 각 부위별 현존량 분배율은 줄기>가지>수피>잎 순이었으며, 각 부위별 조직내 양분농도는 낙엽송이 리기다소나무에 비해 높았고, 두 임분 모두 잎>가지>수피>줄기 순으로 양분농도에 차이가 있었다. 양분 축적량은 리기다소나무 임분이 질소 335.9kg/ha, 칼슘 188.6kg/ha, 칼륨 121.4kg/ha, 마그네슘 93.8kg/ha, 인산 40.4kg/ha 순이었으며 낙엽송 임분은 질소 225kg/ha, 칼슘 75.7kg/ha, 칼륨 72.9kg/ha, 마그네슘 37.1kg/ha, 인산 11.5kg/ha으로 리기다소나무 임분에서 양분 축적량이 높게 나타났다. 양분에 대한 지상부 현존량의 비로 표시되는 양분이용효율중 질소이용효율은 낙엽송 임분이 리기다소나무 임분에 비해 낮게 나타났으며 이는 리기다소나무가 낙엽송에 비해 척박지에서 더 잘 견딜 수 있는 임목의 특성을 반영하고 있다.

• 한국습지학회지
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• 제9권2호
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• pp.33-43
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• 2007

버드나무속의 분포 현황과 생산성을 조사하여 버드나무속 군집을 보전하고 생태공학적으로 이용하는 데 필요한 기초 자료를 제공하기 위하여 경남 창녕군 우포늪에서 2005년 4월부터 2006년 4월까지 버드나무속의 군집별 분포 면적, 현존식생도, 현존량, 상대생장 및 1차 생산성을 조사하였다. 1. 우포 지역에 분포하는 버들류 7종류 중 선버들이 우점종이고 왕버들이 아우점하였으며, Salix 군락은 선버들, 왕버들, 버드나무, 선버들-왕버들, 왕버들-선버들, 선버들-버드나무 군락 등 총 6종류였다. 2. Salix 군락의 분포 면적은 선버들 군락이 28.46 ha로 가장 넓고, 선버들-왕버들 군락, 왕버들-선버들 군락이 각각 6.12 ha, 선버들-버드나무 군락 2.92 ha, 왕버들 군락 1.86 ha, 버드나무 군락 0.81 ha 등의 순이었다. 3. Salix 군락을 구성하는 선버들은 수령 5~13년, 수고 4.1~7.2 m, DBH 3.0~14.0 cm 등이고, 왕버들은 수령 2~36년, 수고 3.5~10.1 m, DBH 3.2~26 cm 등이었다. 4. 선버들의 현존량은 408 ton 이었으며, 왕버들의 현존량은 336 ton 이었다. 5. 선버들의 연순생산성은 $235g\;m^{-2}yr^{-1}$이고, 이를 우포지역으로 환산한 연순생산량은 $86.4ton\;yr^{-1}$였으며, 왕버들은 $1,006g\;m^{-2}yr^{-1}$ 및 $80.3ton\;yr^{-1}$이었다.

• 농업과학연구
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• 제38권4호
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• pp.785-791
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• 2011

This study was carried out to analyze the effect of the farm management and productivity increase of cabbages growing in the plastic house based on application of green manure crops such as silage corn, haussolgo (Sorghum bicolar L. Moench), hairy vetch, etc. According to the effect of green manure crop grown in summer season, the biomass amount of the silage corn was the highest; 7,630 kg per 10a, the next was haussolgo, 5,620 kg per 10a. In terms of the fertilizer ingredients, the first of forage soybean was 3.84% of nitrogen, whereas hairy vetch was 1.74% of phosphate and kalium, 4.74%. Productivity increase of cabbages was the highest in the haussolgo plot of which the yield of showed 10,090 kg per 10a and the farm household income would be worth 8,053 thousand won. By growing forage crops in the winter season, the biomass amount was the highest in the mixed sowing plot with rye (50%) and hairy vetch (50%) of 3,590 kg per 10a, whereas the productivity in the mixed seeding with rye (70%) and hairy vetch (30%) was highest, 6,249 kg per 10a and the farm household income would be worth 7,387 thousand won. Judging from these results, more practical on-farm research on applying different green manure crops as a basal fertilizer with cabbage in the plastic house should be considered to analyze the farm management and the farm household income at different sites.

• The Korean Journal of Ecology
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• 제24권6호
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• pp.377-380
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• 2001

Herbivory is an important selective forces on plants by reducing the ability of the plant to compete with its neighbors and to produce offspring. Oak nut weevil(Mechoris ursulus Roelofs) females lay eggs in unmatured acorns and cut off the branch of oviposited acorns. To investigate the influences of branch-cutting behavior of oak nut weevils on oak production, we surveyed the horizontal and vertical distribution ranges of damaged oaks and depth of sites of overwintering larvae and quantified the amounts of biomass and acorn removed by the weevils on deciduous oak species. All of the endemic oak species in Korea were damaged by oak nut weevils in all of the study sites including Mt. Halla and Mt. Seorak. The upper limit of vertical distribution of damaged oaks by the weevils raried from 700 m to 900 m. Mostly their larvae were found in soil from 8.1 cm to 10.0 cm depth in winter. Percentage of annual net primary productivity($720g{\cdot}m^{-2}{\cdot}day^{-1}$) of oak species removed by the weevils was about 7%, which is higher than the percentage removed by all the herbivores in typical temperate forest(5%) and equivalent to that by all the herbivores in tropical forest. The predation percentage of annual acorn production by oak nut weevil was 27% in Q. mongolica and 33% in Q. acutissima. This results indicate that the branch-cutting behavior of oak nut weevil may be the most important factor regulating oak population and affecting other predators dependent on acorns.

• 한국해양바이오학회지
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• 제10권1호
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• pp.26-33
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• 2018

Fucoxanthin has been reported as bioactive compounds exhibiting strong antioxidant, anticancer and anti-inflammatory activities. Owing to its a wide range of applications and potentials, commercial production of fucoxanthin from algae has been attracted many attentions. Although, most of seaweeds and diatoms contain fucoxanthin as major carotenoid contents, low productivity of fucoxanthin still hinder the industrial application. Here, we newly isolated and identified indigenous marine diatom Odontella sp. BS-003 as a resource of fucoxanthin production. The characterization, optimization and production of the fucoxanthin, along with other bioactive compound omega-3 fatty acid from odontella sp. BS-003 were analyzed in this study, and the results represented optimal culture condition (two-fold silicate containing F/2 medium) significantly enhanced the algal biomass productivity. The maximum biomass (1.83 g/L), fucoxanthin (3.88 mg/g), along with omega-3 fatty acid (10 %, w/w) were obtained from the 10 L of photobioreactor. Based on the results, it is speculated that the microalga Odontella sp. BS-003 can be a promising natural resource for the production of bioactive compounds.

• The Korean Journal of Ecology
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• 제1권2호
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• pp.57-65
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• 1977

The biomass and net production of alder and oak trees was estimated by allometric method. The productivity of the two stands of alder and oak was obviously different judging from the rate of photosynthesis productive structure and vertical distribution of light. The amounts of net photosynthesis under the saturated light were 2.31, 1.42mg $CO_2/dm^2\cdot$hr. in the sun and shade leaves of alder tree and 1.58, 0.84mg $CO_2/dm^2\cdot$hr in that of the oak, respectively. Total annual respiration loss calculated from the respiration measured at $25^{\circ}C$ and the mean air temperature from every 10 days were 13.56ton/ha.yr in the alder stand and 19.83 ton/ha.yr in the oak. The productive structure and the vertical distribution of light in the stand were assumedly more effective to produce dry matter in the oak stand than in the alder. The biiomasses measured in 1975 and 1976 were 51.51 and 56.82 ton/ha in the alder stand and 73.35, 86.77 ton/ha in the oak one, respectively. Annual net production and gross production were 8.56 and 22.12 ton/ha.yr in the alder stand but those were 17.90 and 37.74 ton/ha.yr in the oak stand. The ratios of respiration to gross procution (R/Pg) were prespectively 0.61 and 0.53 inthe alder and oak stands. Efficiencies of solar energy utilizaztion of net production during the growing season(May-Oct.) were 0.67 and 1.40% and those of gross production were 1.72 and 2.94% in the alder and oak stands respectively.

• 한국해양바이오학회지
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• 제11권1호
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• pp.14-22
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• 2019

Omega-3 fatty acids and carotenoids, which are known as representative high-value substances derived from microalgae, are being studied from various diatoms. Most of the diatoms contain fucoxanthin and omega-3 fatty acid. Fucoxanthin produced by diatom has been reported as bioactive compounds exhibiting strong antioxidant, anticancer and anti-inflammatory activities. However, the low growth rate and fucoxanthin content of diatoms are one of the big obstacles to the industrial application. In this study, indigenous marine diatom Achnanthidium sp. BS-001 was isolated for a candidate of fucoxanthin producer. Light intensity and temperature for the culture of Achnanthidium sp. BS-001 were optimized on PhotoBiobox. Optimization of silicate concentration for increasing BS-001 biomass productivity was confirmed in F/2 medium with various concentration of sodium silicate. As a result, condition of light intensity, temperature, and silicate concentration for optimal cultivation were $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, $18^{\circ}C$ and 0.106 mM, respectively. Maximum biomass productivity reaches to $154.3mg{\cdot}L^{-1}{\cdot}day^{-1}$, and then the content of omega-3 fatty acids and fucoxanthin were $19.4mg{\cdot}g^{-1}$, $9.05mg{\cdot}g^{-1}$, respectively. These results indicate that Achnanthidium sp. BS-001 has the potential to be used as a source of omega-3 fatty acids and fucoxanthin.

• Journal of Microbiology and Biotechnology
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• 제32권10호
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• pp.1325-1334
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• 2022

Global warming has accelerated in recent decades due to the continuous consumption of petroleum-based fuels. Cyanobacteria-derived biofuels are a promising carbon-neutral alternative to fossil fuels that may help achieve a cleaner environment. Here, we propose an effective strategy based on the large-scale cultivation of a newly isolated cyanobacterial strain to produce phycobiliprotein and biodiesel, thus demonstrating the potential commercial applicability of the isolated microalgal strain. A native cyanobacterium was isolated from Goryeong, Korea, and identified as Pseudanabaena mucicola GO0704 through 16s RNA analysis. The potential exploitation of P. mucicola GO0704 was explored by analyzing several parameters for mixotrophic culture, and optimal growth was achieved through the addition of sodium acetate (1 g/l) to the BG-11 medium. Next, the cultures were scaled up to a stirred-tank bioreactor in mixotrophic conditions to maximize the productivity of biomass and metabolites. The biomass, phycobiliprotein, and fatty acids concentrations in sodium acetate-treated cells were enhanced, and the highest biodiesel productivity (8.1 mg/l/d) was achieved at 96 h. Finally, the properties of the fuel derived from P. mucicola GO0704 were estimated with converted biodiesels according to the composition of fatty acids. Most of the characteristics of the final product, except for the cloud point, were compliant with international biodiesel standards [ASTM 6761 (US) and EN 14214 (Europe)].

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권6호
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• pp.482-493
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• 2005

Biomass is originally photosynthesized from inorgainic compounds such as $CO_2$, minerals, water and solar energy. Recent studies have shown that anaerobic bacteria have the ability to convert recalcitrant biomass such as cellullosic or chitinoic materials to useful compounds. The biomass containing agricultural waste, unutilized wood and other garbage is expected to utilize as feed, food and fuel by microbial degradation and other metabolic functions. In this study we isolated several anaerobic, cellulolytic and chitinolytic bacteria from rumen fluid, compost and soil to study their related enzymes and genes. The anaerobic and cellulolytic bacteria, Clostridium thermocellum, Clostridium stercorarium, and Clostridium josui, were isolated from compost and the chitinolytic Clostridium paraputrificum from beach soil and Ruminococcus albus was isolated from cow rumen. After isolation, novel cellulase and xylanase genes from these anaerobes were cloned and expressed in Escherichia coli. The properties of the cloned enzymes showed that some of them were the components of the enzyme (cellulase) complex, i.e., cellulosome, which is known to form complexes by binding cohesin domains on the cellulase integrating protein (Cip: or core protein) and dockerin domains on the enzymes. Several dockerin and cohesin polypeptides were independently produced by E. coli and their binding properties were specified with BIAcore by measuring surface plasmon resonance. Three pairs of cohesin-dockerin with differing binding specificities were selected. Two of their genes encoding their respective cohesin polypeptides were combined to one gene and expressed in E. coli as a chimeric core protein, on which two dockerin-dehydrogenase chimeras, the dockerin-formaldehyde dehydrogenase and the dockerin-NADH dehydrogenase are planning to bind for catalyzing $CO_2$ reduction to formic acid by feeding NADH. This reaction may represent a novel strategy for the reduction of the green house gases. Enzymes from the anaerobes were also expressed in tobacco and rice plants. The activity of a xylanase from C. stercorarium was detected in leaves, stems, and rice grain under the control of CaMV35S promoter. The digestibility of transgenic rice leaves in goat rumen was slightly accelerated. C. paraputrificum was found to solubilize shrimp shells and chitin to generate hydrogen gas. Hydrogen productivity (1.7 mol $H_2/mol$ glucos) of the organism was improved up to 1.8 times by additional expression of the own hydrogenase gene in C. paraputrficum using a modified vector of Clostridiu, perfringens. The hydrygen producing microflora from soil, garbage and dried pelletted garbage, known as refuse derived fuel(RDF), were also found to be effective in converting biomass waste to hydrogen gas.