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http://dx.doi.org/10.15433/ksmb.2018.10.1.026

Isolation and Characterization of Indigenous Diatom, Odontella sp. BS-003 as Potential Fucoxanthin and Omega-3 Fatty Acid Producer  

Heo, Jina (Korea Research Institute of Bioscience and Biotechnology(KRIBB) Cell Factory Research Center)
Cho, Dae-Hyun (Korea Research Institute of Bioscience and Biotechnology(KRIBB) Cell Factory Research Center)
Kim, Urim (Korea Research Institute of Bioscience and Biotechnology(KRIBB) Cell Factory Research Center)
Kim, Hee-Sik (Korea Research Institute of Bioscience and Biotechnology(KRIBB) Cell Factory Research Center)
Publication Information
Journal of Marine Bioscience and Biotechnology / v.10, no.1, 2018 , pp. 26-33 More about this Journal
Abstract
Fucoxanthin has been reported as bioactive compounds exhibiting strong antioxidant, anticancer and anti-inflammatory activities. Owing to its a wide range of applications and potentials, commercial production of fucoxanthin from algae has been attracted many attentions. Although, most of seaweeds and diatoms contain fucoxanthin as major carotenoid contents, low productivity of fucoxanthin still hinder the industrial application. Here, we newly isolated and identified indigenous marine diatom Odontella sp. BS-003 as a resource of fucoxanthin production. The characterization, optimization and production of the fucoxanthin, along with other bioactive compound omega-3 fatty acid from odontella sp. BS-003 were analyzed in this study, and the results represented optimal culture condition (two-fold silicate containing F/2 medium) significantly enhanced the algal biomass productivity. The maximum biomass (1.83 g/L), fucoxanthin (3.88 mg/g), along with omega-3 fatty acid (10 %, w/w) were obtained from the 10 L of photobioreactor. Based on the results, it is speculated that the microalga Odontella sp. BS-003 can be a promising natural resource for the production of bioactive compounds.
Keywords
Microalgae; Odontella sp.; Optimization; Fucoxanthin; Omega-3 fatty acid;
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1 Peng, Juan, et al. "Fucoxanthin, a marine carotenoid present in brown seaweeds and diatoms: metabolism and bioactivities relevant to human health." Marine drugs 9.10 (2011): 1806-1828.   DOI
2 Pangestuti, Ratih, and Se-Kwon Kim. "Biological activities and health benefit effects of natural pigments derived from marine algae." Journal of functional foods 3.4 (2011): 255-266.   DOI
3 Dembitsky, Valery M., and Takashi Maoka. "Allenic and cumulenic lipids." Progress in Lipid Research 46.6 (2007): 328-375.   DOI
4 Kumar, Chandini S., et al. "Seaweeds as a source of nutritionally beneficial compounds-a review." Journal of Food Science and Technology 45.1 (2008): 1-13.
5 Hosokawa, Masashi, et al. "Bio-functions of marine carotenoids." Food Science and Biotechnology 18.1 (2009): 1-11.
6 Sachindra, Nakkarike M., et al. "Radical scavenging and singlet oxygen quenching activity of marine carotenoid fucoxanthin and its metabolites." Journal of agricultural and food chemistry 55.21 (2007): 8516-8522.   DOI
7 Heo, Soo-Jin, et al. "Evaluation of anti-inflammatory effect of fucoxanthin isolated from brown algae in lipopolysaccharide-stimulated RAW 264.7 macrophages." Food and Chemical Toxicology 48.8-9 (2010): 2045-2051.   DOI
8 Jimenez‐Escrig, Antonio, et al. "Antioxidant activity of fresh and processed edible seaweeds." Journal of the Science of Food and Agriculture 81.5 (2001): 530-534.   DOI
9 Urikura, Itaru, Tatsuya Sugawara, and Takashi Hirata. "Protective effect of fucoxanthin against UVB-induced skin photoaging in hairless mice." Bioscience, biotechnology, and biochemistry 75.4 (2011): 757-760.   DOI
10 Beppu, Fumiaki, et al. "Down‐regulation of hepatic stearoyl‐CoA desaturase‐1 expression by Fucoxanthin via leptin signaling in diabetic/obese KK‐Ay mice." Lipids 48.5 (2013): 449-455.   DOI
11 Satomi, Yoshiko. "Antitumor and Cancer-preventative Function of Fucoxanthin: A Marine Carotenoid." Anticancer research 37.4 (2017): 1557-1562.   DOI
12 S. Y. Kang, H. Kang, J. E. Lee, C. S. Jo, C. B. Moon, J. Ha, J. S. Hwang, J. Choi, "Anti-aging potential of fucoxanthin concentrate derived from Phaeodactylum tricornumtum", J. Cosmet. sci., submitted, 2017.
13 Xia, Song, et al. "Production, characterization, and antioxidant activity of fucoxanthin from the marine diatom Odontella aurita." Marine drugs 11.7 (2013): 2667-2681.   DOI
14 WANG, Wen‐Jun, et al. "Isolation of fucoxanthin from the rhizoid of Laminaria japonica Aresch" Journal of Integrative Plant Biology 47.8 (2005): 1009-1015   DOI
15 Kanazawa, Kazuki, et al. "Commercial-scale preparation of biofunctional fucoxanthin from waste parts of brown sea algae Laminalia japonica." Food science and technology research 14.6 (2008): 573-573.   DOI
16 Kim, Sang Min, Ya Fang Shang, and Byung‐Hun Um. "A preparative method for isolation of fucoxanthin from Eisenia bicyclis by centrifugal partition chromatography." Phytochemical analysis 22.4 (2011): 322-329.   DOI
17 Kim, Sang Min, et al. "A potential commercial source of fucoxanthin extracted from the microalga Phaeodactylum tricornutum." Applied biochemistry and biotechnology 166.7 (2012): 1843-1855.   DOI
18 Cho, Dae‐Hyun, et al. "Novel approach for the development of axenic microalgal cultures from environmental samples." Journal of Phycology 49.4 (2013): 802-810.   DOI
19 Pulz, Otto, and Wolfgang Gross. "Valuable products from biotechnology of microalgae." Applied microbiology and biotechnology 65.6 (2004): 635-648.   DOI
20 Mimouni, Virginie, et al. "Marine microalgae used as food supplements and their implication in preventing cardiovascular diseases." OCL 22.4 (2015): D409.   DOI
21 Zimmermann, Christine, et al. "A chemical-genetic screen to unravel the genetic network of CDC28/CDK1 links ubiquitin and Rad6-Bre1 to cell cycle progression." Proceedings of the National Academy of Sciences 108.46 (2011): 18748-18753.   DOI
22 Guillard, Robert RL. "Culture of phytoplankton for feeding marine invertebrates." Culture of marine invertebrate animals. Springer, Boston, MA, 1975. 29-60.
23 Luhana, K. K., and H. D. Patel. "Cultural and molecular characterization of tetracycline resistant microflora associated with dental caries." International Journal of Genetics 2.1 (2010): 1.   DOI
24 Peters, Adam, et al. "Development of biotic ligand models for chronic manganese toxicity to fish, invertebrates, and algae." Environmental Toxicology and Chemistry 30.11 (2011): 2407-2415.   DOI
25 Wayama, Marina, et al. "Three-dimensional ultrastructural study of oil and astaxanthin accumulation during encystment in the green alga Haematococcus pluvialis." PloS one 8.1 (2013): e53618.   DOI
26 Bligh, E. Graham, and W. Justin Dyer. "A rapid method of total lipid extraction and purification." Canadian journal of biochemistry and physiology 37.8 (1959): 911-917.   DOI
27 윤지현; 곽인규; 진언선. 효소, 대사산물; 고광도와 질소 결핍이 Haematococcus pluvialis의 색소 생합성에 미치는 영향. 한국미생물.생명공학회지, 2007, 35.4: 292-297.
28 CHO, Dae-Hyun, et al."Enhancing microalgal biomass productivity by engineering a microalgal-bacterial community" Bioresource technology 175 (2015): 578-585.   DOI