• International Journal of Contents
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• v.8 no.4
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• pp.74-77
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• 2012

Radiation has been an effective tool for treating cancer for a long time. Radiation therapy induces DNA damage within cancer cells and destroys their ability to reproduce. Radiation therapy is often combined with other treatments, like surgery and chemotherapy. Here, we describe the effects of radiation and histone deacetylase inhibitor, Trichostain A, on cell cycle regulation in hepatoma cells. The combinatorial treatment of radiation and Trichostain A induced cell cycle arrest and thereby increasing the hepatoma cell death. Furthermore, the regulatory effects of radiation and Trichostatin A on cell cycle applied in cell type specifically. These results suggest that the treatment of radiation and Trichostatin A may play a central role in hepatoma cell death and might be a good remedy to improve the efficiency of radiation therapy.

• Animal cells and systems
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• v.10 no.3
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• pp.125-130
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• 2006

We have examined the bioaccumulation patterns and the ecophysiological responses (photosynthetic pigment and total antioxidative capacity) of Monochoria korsakowi exposed to various cadmium concentrations, one of major environmental pollutants. Cadmium ion contents in M. korsakowi increased significantly with higher cadmium concentration, and most of the accumulated cadmium was found in the root parts. Biomass of each part decreased with higher cadmium concentration. As cadmium treatment concentration was increased, chlorophyll a content was decreased, whereas chlorophyll b content was increased. However, the variations of total chlorophyll and carotenoid contents were not evident. Total antioxidative capacity in the leaves of cadmium treated M. korsakowi increased greatly with higher cadmium concentration. We considered these results as indicative of the ability of M. Korsakowi plants to take up cadmium from wetlands.

• Journal of Plant Biology
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• v.32 no.4
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• pp.215-226
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• 1989

The effects of Ca2+ on polyamines on callose contents of carrot suspension cultured cells were studied. The regeneration process of the cell wall of carrot protoplast observed through the electron microscope. Treatment of the carrot suspension cultured cells with Ca2+ and polyamines resulted in considerable increase on callose contents at 0.1 mM of Ca2+ and polyamines, particulary spermidine. Poly-L-lysine and poly-L-ornithine increased about 30% and 100% of callose contents than that of the control respectively, whereas verapamil and flunarizine markedly decreased the callose contents. These effects of Ca2+ of free ion rather than as Ca2+-calmodulin complex. During the cultivation of the protoplast, the regeneration of the cell wall was somewhat observed on the 4th day, however, it was inhibited by verapamil. These results suggested that the promotive action of Ca2+ and polyamines were manifested in the callose contents and the regeneraton of the cell wall.

• Animal cells and systems
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• v.1 no.3
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• pp.507-513
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• 1997

Sequential observations of binding patterns and structural effects of Bacillus thuringiensis var. kurstaki were made on fat body tissue of the fall webworm, Hyphantria cunea Drury. Fat body was cultured in vitro in the presence of purified 62 kDa endotoxin and then examined for protein synthesis and the localization of membrane-bound toxin detected by an antibody against the 62 kDa endotoxin. Protein synthesis was mostly inhibited at concentrations of 15 ${\mu}$g/ml and higher. Immunocytochemical observations suggest that the toxin binds to all exposed basal lamina surrounding the fat body without apparent specificity. The cytopathic effect delectable by scanning electron microscope is disintegration rather than cell swelling. The basal lamina bound toxin was eventually detached from the fat body and followed by an extrusion of cell contents like lipid granules.

• The Korean Journal of Ecology
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• v.13 no.3
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• pp.215-224
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• 1990

The distribution of Spirodela polyrhiza and Lemna aequinoctialis was investigated and the limiting factors on the distribution were analyzed. At 66 sites in Korea, the presense or absense of duckweed were recorded and the water was sampled. The temperature, hardness, pH and contents of N, , , Na, K, Ca, Cu, Mg, Zn, Fe, Ni, Mn and Cd of water sampled were analyzed. The results were as follows; It seemed that the limiting factors influencing on the distribution was water temperature and concentration of . The critical temperature of the distribution of Spirodela and Lemna was presented 19.5$^{\circ}C$ and concentration of was limiting factor only in the distribution of S. polyrhiza. L. aequinoctialis was distributed in lower temperature than S. polyrhiza and it seemed that the distribution of L. aequinoctialis was not be influenced by the concentration of .

• Biomedical Science Letters
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• v.16 no.4
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• pp.265-269
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• 2010

Puer tea is a traditional beverage originating from Yunnan area of China. We have analyzed 11 different commercial tea brands provided by Daboo Culture and Art Center. This study was carried out to evaluate the contents of polyphenols, antibacterial activity, antioxidantive ability and physiological activities of extracts from Puer tea. The electron donating ability was ranged from 57.26~99.16% and SOD-like activity was ranged from 1.4~10.4%. The inhibitory effect on the growth of cancer cell lines was examined by MIT assay. The Puer tea extract exhibited the greatest inhibitory effect at the concentration of 2% for all cancer cells tested.

• BMB Reports
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• v.29 no.4
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• pp.343-352
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• 1996

The biosynthesis of phospholipid and the composition of fatty acid were analyzed in mitochondria isolated from Chlorella ellipsoidea treated with carbon sources (glucose, sucrose, raffinose) during the culture. The growth of Chlorella and total lipid contents in mitochondria treated with various carbon sources was increased to compare with the control. When Chlorella mitochondria was treated with various carbon sources, four kinds of phospholipid were increased predominantly. The major fatty acids utilized for the biosynthesis of the phospholipid were analyzed linoleic acid (average 25.18%) and stearic acid (average 10.52%) in the control. But, it was shown that the major fatty acids in Chlorella mitochondria treated with glucose were stearic acid (average 30.93%), palmitic acid (average 17.47%) and stearic acid (average 20.31%), linoleic acid (average 16.68%) in sucrose treatment and oleic acid (average 17.17%), palmitic acid (average 15.64%) in raffinose treatment.

• Journal of Applied Biological Chemistry
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• v.43 no.3
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• pp.165-169
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• 2000

Regarding to maturity evaluation of pig manure compost mixed with saw dust, change of constituents of organic matter influenced by microbial activities were investigated. Throughout the two stages of active composting period, we obtained a lot of data related to compost stabilization. However, we found out that only a couple of parameters could be used for adequate evaluation of compost. We, therefore, decided that total sugar and reducing sugar could be used for the reasonable standard criteria of maturity during composting process, even though some enzyme activities by phosphates and cellulase reactions were obtained and compared. Because the other parameters such as contents of lignin, cellulose, and organic acids were difficult to be used for maturity evaluation of pig manure compost.

• Proceedings of the Zoological Society Korea Conference
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• 1998.10b
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• pp.169.2-169
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• 1998

No Abstract, See Full Text

• Natural Product Sciences
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• v.14 no.3
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• pp.187-191
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• 2008

The contents of isoflavones and 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activities were determined in flowers (Puerariae Flos) and roots (Puerariae Radix) of Pueraria thunbergiana to differentiate both pharmacochemical or pharmacognostical usage of both oriental medicinal drugs. The tectorigenin contents in flower before and after acid hydrolysis were shown to be 17.10 ${\mu}mole\;g^{-1}$ and 49.58 ${\mu}mole\;g^{-1}$, respectively, analyzed by HPLC. However, the root displayed much less content of tectorigenin even after acid hydrolysis (6.56 ${\mu}mole\;g^{-1}$) than in the flower. Acid hydrolysis made glycitin and tectoridin of the isoflavone glycosides almost disappear in the flower and root. DPPH assay results demonstrated that acid hydrolysis of both extracts or both glycosides should increase free radical-scavenging activities due to the increase of isoflavone contents. It is also suggested that the flower had much higher concentration of isoflavonoids than in the root.