• 한국어류학회지
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• 제24권1호
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• pp.1-10
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• 2012

미꾸라지, 미꾸리 및 상반교배를 통해 유도된 종간 잡종의 세포유전학적 분석을 수행하였다. 미꾸라지와 미꾸리의 염색체 수는 각각 2n=48 (12M+4SM+32A), 2n=50 (10M+4SM+36A)이었고, 잡종군들의 염색체수는 각각 2n=49 (11M+4SM+34A)였다. 모든 그룹의 염색체는 동일한 arm number (NF=64)를 갖고 있었으며, 염색체 다형현상, 암수 간 이형의 염색체는 관찰되지 않았다. 적혈구의 크기, DNA 함량을 분석한 결과 잡종군들은 미꾸라지와 미꾸리의 중간 값을 나타냈다. 염색체의 NORs (nucleolar organizing regions)은 모두 동일한 중부염색체 단완부에서 Ag-positive signal이 나타났다. 이상의 결과는 미꾸라지의 1번 중부 염색체와 미꾸리의 차단부 염색체가 Robertsonian 형의 염색체 전좌 과정을 거쳤을 것을 시사한다.

• Journal of Ginseng Research
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• 제42권1호
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• pp.98-106
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• 2018

Panax ginseng has been used since ancient times based on the traditional Asian medicine theory and clinical experiences, and currently, is one of the most popular herbs in the world. To date, most of the studies concerning P. ginseng have focused on specific mechanisms of action of individual constituents. However, in spite of many studies on the molecular mechanisms of P. ginseng, it still remains unclear how multiple active ingredients of P. ginseng interact with multiple targets simultaneously, giving the multidimensional effects on various conditions and diseases. In order to decipher the systems-level mechanism of multiple ingredients of P. ginseng, a novel approach is needed beyond conventional reductive analysis. We aim to review the systems-level mechanism of P. ginseng by adopting novel analytical framework-network pharmacology. Here, we constructed a compound-target network of P. ginseng using experimentally validated and machine learning-based prediction results. The targets of the network were analyzed in terms of related biological process, pathways, and diseases. The majority of targets were found to be related with primary metabolic process, signal transduction, nitrogen compound metabolic process, blood circulation, immune system process, cell-cell signaling, biosynthetic process, and neurological system process. In pathway enrichment analysis of targets, mainly the terms related with neural activity showed significant enrichment and formed a cluster. Finally, relative degrees analysis for the target-disease association of P. ginseng revealed several categories of related diseases, including respiratory, psychiatric, and cardiovascular diseases.

• The Plant Pathology Journal
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• 제21권1호
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• pp.47-54
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• 2005

Identification of host genes involved in disease progresses and/or defense responses is one of the most critical steps leading to the elucidation of disease resistance mechanisms in plants. Soybean mosaic virus (SMV) is one of the most prevalent pathogen of soybean (Glycine max). Although the soybeans are placed one of many important crops, relatively little is known about defense mechanism. In order to obtain host genes involved in SMV disease progress and host defense especially for virus resistance, two different cloning strategies (DD RT-PCR and Subtractive hybridization) were employed to identify pathogenesis- and defenserelated genes (PRs and DRs) from susceptible (Geumjeong 1) and resistant (Geumjeong 2) cultivars against SMV strain G7H. Using these approaches, we obtained 570 genes that expressed differentially during SMV infection processes. Based upon sequence analyses, differentially expressed host genes were classified into five groups, i.e. metabolism, genetic information processing, environmental information processing, cellular processes and unclassified group. A total of 11 differentially expressed genes including protein kinase, transcription factor, other potential signaling components and resistant-like gene involved in host defense response were selected to further characterize and determine expression profiles of each selected gene. Functional characterization of these genes will likely facilitate the elucidation of defense signal transduction and biological function in SMV-infected soybean plants.

• 한국인터넷방송통신학회논문지
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• 제16권3호
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• pp.203-211
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• 2016

유전자 코드는 바이오 정보 처리에 키 포인트로 인체의 유기적인 조직체이다. 현대 과학에서는 유전자 코드 분자구조의 신비스러운 특성을 체계적으로 설명하고 이해하는데 연구가 집중되고 있다. 본 논문에서는 유전자 시스템을 대칭적으로 해석하는데 중점을 두었고, Jacket 행렬로 무잡음 RNA 유전자 코드를 가장 단순하게 해석했다. 이유는 Jacket 행렬과 RNA는 그 역행렬이 Element (Block)-wise Inverse로 그 역(Inverse)도 자신이란 점과 대칭적 성질, 그리고 Kronecker곱을 갖기 때문이다. 제안된 방법이 유전자 RNA 코돈(Codon : 괘(卦))의 견지에서 Jacket 행렬의 분해를 통해 간단하고 명료함을 보인다.

• 대한본초학회지
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• 제30권3호
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• pp.13-17
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• 2015

Objectives : Taraxacum platycarpum H. Dahlstedt has been reported to have several biological properties such as skin hydration and antiinflammation. The purpose of this study was to examine the antidepressive effects of water extract of T. platycarpum (WTP) on an animal model of depression. Methods : In the present study, normal ICR mice (4 weeks) were used, and orally administered with WTP (25, 50 and 100 mg/kg). Depression-like behavior was monitored the forced swimming test (FST) and tail suspension test (TST) in mice. The locomotor activity was evaluated to eliminate the false-positive activity in the open field test (OFT). Fluoxetine, the selective serotonin reuptake inhibitor, as a positive control was intraperitoneally administered at a dose of 15 mg/kg at 30 min before starting the behavioral test. Moreover, we evaluated the effects of WTP on the expression of brain-derived neurotrophic factor (BDNF) and the extracellular signal-regulated kinase (ERK)/ cyclic AMP response-element binding protein (CREB) signaling pathway in the hippocampus using Western blot. Results : The administration of WTP (50 and 100 mg/kg) significantly (P < 0.05, respectively) reduced the immobility time during FST and TST without accompanying changes in locomotor activity by OFT. Furthermore, WTP at dose of 100 mg/kg increased the BDNF expression and the phosphorylation of ERK and CREB in the hippocampus region. Conclusions : These results suggest that WTP has a useful anti-depressant effect through the regulation of BDNF/ERK/CREB signaling pathway.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권5호
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• pp.442-448
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• 2006

Human lactoferrin (hLf) is an iron-binding glycoprotein that has been considered to play many biological roles in the human, including the stimulation of the immune system, antimicrobial and anti-inflammatory effects, and regulation of iron absorption. We generated transgenic Siberian ginseng (Acanthopanax senticosus) cell cultures producing a functional hLf protein using the signal peptide sequence from the endoplasmic reticulum and driven by an oxidative stress-inducible SWPA2 promoter which is highly expressed in plant cell cultures. The production of hLf increased proportionally to cell growth and showed a maximal level (up to 3.6% of total soluble protein) at the stationary phase in suspension cultures. Full-length hLf protein was identified by immunoblot analysis in transgenic cell cultures of Siberian ginseng. Recombinant hLf (rhLf) was purified from suspension cells of Siberian ginseng by ammonium sulfate precipitation, cation-exchange and gel filtration chromatography. N-terminal sequences of rhLf were identical to native hLf (nhLf). The overall monosaccharide composition of rhLf showed the presence of plant specific xylose while sialic acid is absent. Antibacterial activity of purified rhLf was higher than that of nhLf. Taken together, we anticipate that medicinal Siberian ginseng cultured cells, as demonstrated by this study, will be a biotechnologically useful source for commercial production of functional hLf not requiring further purification.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2017년도 춘계학술대회 논문집
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• pp.156-156
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• 2017

In this work, analysis of biocompatible TiO2 oxide multilayer by the XPS depth profiling was researched. the manufacture of the TiO2 barrier-type multilayer was accurately performed in a mixed electrolyte containing HAp, Pd, and Ag nanoparticles. The temperature of the solution was kept at approximatively $32^{\circ}C$ and was regularly rotated by a magnetic stirring rod in order to increase the ionic diffusion rate. The manufactured specimens were carefully analyzed by XPS depth profile to investigate the result of chemical bonding behaviors. From the analysis of chemical states of the TiO2 oxide multilayer using XPS, the peaks are showed with the typical signal of Ti oxide at 459.1 eV and 464.8 eV, due to Ti 2p(3/2) and Ti 2p(1/2), respectively. The Pd-3d peak was split into Pd-3d(5/2) and Pd-3d(3/2)peaks, and shows two bands at 334.7 and 339.9 eV for Pd-3d3 and Pd-3d5, respectively. Also, the peaks of Ag-3d have been investigated. The chemical states consisted of the O-1s, P-2p, and Ti-2p were identified in the forms of PO42- and PO43-. Based on the results of the chemical states, the chemical elements into the TiO2 oxide multilayer were also inferred to be penetrated from the electrolyte during anodic process.The structure characterization of the modified surface were performed by using FE-SEM, and from the result of biological evaluation in simulated body fluid(SBF), the biocompatibility of TiO2 oxide multilayer was effective for bioactive property.

• 대한의용생체공학회:의공학회지
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• 제23권2호
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• pp.87-99
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• 2002

본 논문에서는 대상테의 움직임이 합성구경 기법에 미치는 영향을 조사하고, 이를 토대로 합성구경 기법의 움직임 긱함을 극복할 수 있는 방법을 제안하였다. 제안한 방법은 일정한 관심영역 내에서 대상테의 대표적인 축방향 움직임을 추정하고 이에 의한 집속 지연 오타만을 관심영역의 모든 영상점에 대하여 동일하게 보상하는 영역 기반의 움직임 보상 기법을 사용한다. 이때 대표 움직임은 관심영역 내에서 접속된 신호의 에너지가 최대가 되는 점에서의 움직임을 상호상관을 이용하여 계산할 수 있다. 또한 자기상관 기반의 도플러 평균 주파수 추정 기법을 이용하여 관심영역 내의 대표적인 축방향 움직임(대표 움직임 속도)을 추정하는 방법을 제안하였다. 모의 실험과 실제 인체 실험 결과 두 방법 모두 합성구경 영상의 움직임 오류를 제거함으로써 양방향 동적접속의 구현을 가능하게 하여 초음파 영상의 해상도 및 SNR을 현저히 개선시킬 수 있음을 확인하였다. 또한 자기상관 방법은 훨씬 적은 계산량으로 상호상관 방법과 거의 동일한 성능을 제공함을 확인하였다.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1989년도 춘계학술대회
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• pp.51-54
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• 1989

지난 몇년동안 생체에서의 MR 스펙트로 스코피(Mangetic Resonance Spectroscopy)를 위하여 ISIS, VSE, SPARS, STEAM 등과 같은 여러 체적 선택의 방법이 연구되어 왔다. 그러나 이들 3차윈 체적 선택의 방법은 여러 RF 펄스를 사용하여야 되었으며 상대적으로 오랜시간 동안의 RF여기시간(Radio frequency excitation time) 때문에 T1과 T2 감소효과의 영향을 크게 받았다. 본 논문에서는 3차원의 체적을 선택하기 위하여 단일 RF펄스의 사용을 제안하고 있는데, 이 방법은 원통형 체적을 선택하기 위한 방사형 경사 코일 (a radial gradient coil) 방법과 진동자계 (oscillating selection gradient) 방법을 결합하고 있다. 이렇게 하므로 단일 RF 펄스만을 사용하여 체적 선택 시간을 최소화하여 여러 RF 펄스를 사용할 경우 발생하는 파생적인 에코 신호(echo signal)를 피할 수 있었다. 제안된 방법에 의한 실험적 연구결과로서 펜톰(phantom)에 대하여 선택된 체적에 대한 영상과 스펙트럼 (spectrum)을 제시하였다.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1997년도 추계학술대회
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• pp.63-66
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• 1997

With the advent of high speed digital signal processing chips, new digital techniques have been introduced to the hearing instrument. This advanced hearing instrument circuitry has led to the need or and the development of new fitting approach. A number of different fitting approaches have been developed over the past few years, yet there has been little agreement on which approach is the "best" or most appropriate to use. However, when we develop not only new hearing aid, but also its fitting method, the intensive subject-based clinical tests are necessarily accompanied. In this paper, we present an objective method to evaluate and predict the performance of hearing aids without the help of such subject-based tests. In the hearing impairment simulation (HIS) algorithm, a sensorineural hearing impairment model is established from auditory test data of the impaired subject being simulated. Also, in the hearing impairment simulation system the abnormal loudness relationships created by recruitment was transposed to the normal dynamic span of hearing. The nonlinear behavior of the loudness recruitment is defined using hearing loss unctions generated from the measurements. The recruitment simulation is validated by an experiment with two impaired listeners, who compared processed speech in the normal ear with unprocessed speech in the impaired ear. To assess the performance, the HIS algorithm was implemented in real-time using a floating-point DSP.