• Title/Summary/Keyword: biological pesticide

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Biological Control of Crown Gall

  • Kerr, Allen;Biggs, John;Ophel, Kathy
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 1994.06a
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    • pp.11-26
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    • 1994
  • Crown gall of stonefruit and nut trees is one of the very few plant diseases subject to efficient biological control. The disease is caused by the soil-inhabiting bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes and the original control organism was a non-pathogenic isolate of A. rhizogenes strain K84. Control is achieved by dipping planting material in a cell suspension of strain K84 which specifically inhibits pathogenic strains containing a nopaline Ti plasmid. Because the agrocin 84-encoding plasmid (pAgK84) is conjugative, it can be transmitted from the control strain to pathogenic strains which, as a result, become immune to agrocin 84 and cannot be controlled. To prevent this happening, the transfer genes on pAgK84 were located and then largely eliminated by recombinant DNA technology. The resulting construct, strain K1026, is transfer deficient but controls crown gall just as effectively as does strain K84. Field data from Spain confirm that pAgK84 can transfer to pathogenic recipients from strain K84 but not from strain K1026. The latter has been registered in Australia as a pesticide and is the first genetically engineered organism in the world to be released fro commercial use. It is recommended as a replacement for strain K84 to prevent a breakdown in the effectiveness of biological control of crown gall. Several reports indicate that both strains K84 and K1026 sometimes control crown gall pathogens that are resistant to agrocin 84. A possible reason for this is that both strains produce a second antibiotic called 434 which inhibits growth of nearly all isolates of A. rhizogenes, both pathogens and non-pathogens. Crown gall of grapevine is caused by another species, Agrobacterium vitis. It is resistant to agrocin 84 and cannot be controlled by strains K84 or K1026. It is different from other crown gall pathogens in several characteristics, including the fact that, although a rhizosphere coloniser, its also lives systemically in the vascular tissue of grapevine. Pathogen free propagating material can be obtained from tissue culture or, less surely, by heat therapy of dormant cuttings. A number of laboratories are searching for a biocontrol strain that will prevent, or at least delay, reinfection. A non-pathogenic A. vitis strain F/25 from South Africa looks very promising in this regard.

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Sinomonas terrae sp. nov., Isolated from an Agricultural Soil

  • Hyosun Lee;Ji Yeon Han;Dong-Uk Kim
    • Journal of Microbiology and Biotechnology
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    • v.33 no.7
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    • pp.909-914
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    • 2023
  • While searching for the bacteria which are responsible for degradation of pesticide in soybean field soil, a novel bacterial strain, designated 5-5T, was isolated. The cells of the strain were Gram-staining-positive, aerobic and non-motile rods. Growth occurred at 10-42℃ (optimum, 30℃), pH 5.5-9.0 (optimum, pH 7.0-7.5), and 0-2% (w/v) NaCl (optimum, 1%). The predominant fatty acids were C15:0 anteiso, C17:0 anteiso, and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). The predominant menaquinone was MK-9 (H2). Diphosphatidylglycerol, glycolipids, phosphatidylinositol, and phosphatidylglycerol were the major polar lipids. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain 5-5T is a member of the genus Sinomonas and its closest relative is Sinomonas humi MUSC 117T, sharing a genetic similarity of 98.4%. The draft genome of strain 5-5T was 4,727,205 bp long with an N50 contig of 4,464,284 bp. Genomic DNA G+C content of strain 5-5T was68.0 mol%. The average nucleotide identity (ANI) values between strain 5-5T and its closest strains S. humi MUSC 117T and S. susongensis A31T were 87.0, and 84.3 % respectively. In silico DNA-DNA hybridization values between strain 5-5T and its closest strains S. humi MUSC 117T and S. susongensis A31T were 32.5% and 27.9% respectively. Based on the ANI and in silico DNA-DNA hybridization analyses, the 5-5T strain was considered as novel species belonging to the genus Sinomonas. On the basis of the results from phenotypic, genotypic and chemotaxonomic analyses, strain 5-5T represents a novel speciesof the genus Sinomonas, for which the name Sinomonas terrae sp. nov. is proposed. The type strain is 5-5T (=KCTC 49650T =NBRC 115790T).

Residual Characteristics of Lambda-cyhalothrin and Deltamethrin in Lettuce (상추 중 Lambda-cyhalothrin과 Deltamethrin의 잔류 특성)

  • Yun, Sang-Soon;Shim, Seok-Won;Kim, Kwang-Ill;Ahn, Myung-Soo;Youn, Teak-Han;Kim, Yun-Jeong;Hwang, Hyo-Seon;Jin, Chung-Woo;Han, Sang-Kuk;Oh, Sang-Kyun;Shin, Jong-Ho;Jin, Yong-Duk;Lee, Eun-Young
    • The Korean Journal of Pesticide Science
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    • v.12 no.2
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    • pp.148-154
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    • 2008
  • This study was carried out to investigate the residue patterns of two insecticides, lambda-cyhalothrin and deltamethrin, commonly used for lettuce, under greenhouse conditions. The pesticides were sprayed with dilution of recommended and doubled doses onto lettuce. Their detection limits were $0.001\;mg{\cdot}kg^{-1}$ and mean recoveries at the fortification levels of 0.2 and $1.0\;mg{\cdot}kg^{-1}$ were from 101.17 to 104.25 and from 99.70 to 103.77%, respectively. The pesticides were gradually decreased in lettuce with time. Biological half-lives of lambda-cyhalothrin and deltamethrin were 1.7 and 1.4 days at the recommended dose and 1.8 and 1.4 days at the doubled dose, respectively. Initial residue amounts of lambda-cyhalothrin and deltamethrin at the recommended and doubled doses exceeded their MRLs. However, the residue levels of the pesticides in the crop sampled at harvest were less than their MRLs. The ratios of the estimated daily intake (EDI) to acceptable daily intake (ADI) by intake the crop harvested 10 days after spraying were less than 1% of their ADIs.

Use of Jumbo Formulation for Paddy-applicable Pesticides (농약 살포작업의 생력화를 위한 투척처리용 점보제의 연구개발에 관하여)

  • Yu, Ju-Hyun;Lim, He-Kyoung;Cho, Kwang-Yun
    • Korean Journal of Environmental Agriculture
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    • v.15 no.1
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    • pp.116-127
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    • 1996
  • Recent development in Japan on jumbo formulation of pesticides for paddy application by hand throw was reviewed. In addition, the diffusion of liquid formulations in water was examined to establish the research strategy for the jumbo formulation. Research on jumbo formulation in Japan has been focused on gas-generating formulations and self-emulsifiable formulations. Although continuous efforts to minimize the problem of pesticide deposit on the treated site have been made for the gas-generating formulations, much work is still needed to establish a generally acceptable formulation method and to commercialize a herbicide formulation. The self-emulsifiable jumbo formulations have recently been investigated. These formulations could simply be processed and showed relatively high biological efficacy. The emulsifiable concentrate was more diffusible than the suspension concentrate in water. The diffusion of the emulsifiable concentrate was not greatly disturbed by floating obstacles. And the diffusion rate was high when the specific grabity was lower than one.

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Construction of a Baculovirus Hyphantria cunea NPV Insecticide Containing the Insecticidal Protein Gene of Bacillus thuringiensis subsp. kurstaki HD1

  • Lee, Hyung-Hoan;Moon, Eui-Sik;Lee, Sung-Tae;Hwang, Sung-Hei;Cha, Soung-Chul;Yoo, Kwan-Hee
    • Journal of Microbiology and Biotechnology
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    • v.8 no.6
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    • pp.685-691
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    • 1998
  • Baculovirus Hyphantrin. cunea nuclear polyhedrosis virus (HcNPV) insecticide containing the insecticidal protein (ICP) gene from Bacillus thuringiensis subsp. kurstaki HD1 was constructed using a lacZ-HcNPV system. The ICP ($\delta$-endotoxin) gene was placed under the control of the polyhedrin gene promoter of the HcNPV. A polyhedrin-negative virus was derived and named ICP-HcNPV insecticide. Then, the insertion of the ICP gene in the ICP-HcNPV genome was confirmed by Southern hybridization analysis. Polyacrylamide gel electrophoresis (PAGE) analysis of the Spodoptera frugiperda cell extracts infected with the ICP-HcNPV showed that the ICP was expressed in the insect cells as 130 kDa at 5 days post-infection. The ICP produced in the cells was present in aggregates. When extracts from the cells infected with the ICP-HcNPV were fed to 20 Bombyx mori larvae, the following mortality rate was seen; 8 larvae at 1 h, 10 larvae at 3 h, and 20 larvae at 12 h. These data indicate that the B. thuringiensis ICP gene was expressed by the baculovirus insecticide in insect cells and there was a high insecticidal activity. The biological activities of the recombinant virus ICP-HcNPV were assessed in conventional bioassay tests by feeding virus particles and ICP to the insect larvae. The initial baculovirus insecticide ICP-HcNPV was developed in our laboratory and the significance of the genetically engineered virus insecticides is discussed.

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Analysis on the Mass-Trapping Effects by Spodoptere litura (Fabricius) (Lepidoptera: Noctuidae) Sex Pheromone Traps Deployed around the Tomato Glasshouse (토마토 유리온실 주변에 설치된 담배거세미나방(Spodoptera litura(Fabricius)) 성페로몬 트랩에 의한 대량포획 효과분석)

  • Kim, Hwang-Yong;Kim, Jeong-Hwan;Son, Byeong-Gwan;Cho, Jum-Rae;Lee, Yong-Hwi;Kim, Yong-Heon;Choi, Man-Young
    • Korean journal of applied entomology
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    • v.48 no.2
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    • pp.245-251
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    • 2009
  • Without pesticide applications, mass-trapping by sex pheromone was successful to control Spodoptera litura (Fabricius) in a large scale tomato glasshouse (10,000 $m^2$) at a low cost of 60 won/$m^2$. Pheromone traps were placed both inside and outside of the glasshouse. Inside the glasshouse traps were installed in a regular space, one trap per 500 $m^2$, to catch the moths that were present in the glasshouse, and traps were also set outside of the glasshouse, at intervals of ca. 20 m, to prevent the moths from invading the glasshouse. In the experiment, more than 400 S. litura were captured per trap.

A Study on Agricultural Safety Technology for Ergonomic Intervention in Farm-work (인간공학적 작업개선을 위한 농업인 안전 기술 동향 연구)

  • Lee, Kyung-Suk;Kim, Hyo-Cher;Chae, Hye-Seon;Kim, Kyung-Ran;Lee, Soo-Jin;Lim, Dae-Seop
    • Journal of the Ergonomics Society of Korea
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    • v.29 no.2
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    • pp.225-239
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    • 2010
  • A high prevalence of farmer's work-related musculo-skeletal disorders (MSDs) have been reported in precedent studies. Risk factors - physical, chemical, biological, ergonomic risk factor etc. - in farm-work were identified by previous studies. To prevent farmers from exposing to them and reduce, Agricultural Safety Technology were introduced and used on farm. The purpose of this study is to review and summarize the agricultural safety technology on farm in Korea. According to strategy principles, this technology is classified as four criteria - workload reduction by reducing awkward posture, workload reduction by using manual material handling aids, Comfort improvement by protecting worker from hot, cold environment and work efficiency improvement by workplace redesign and cultivation modification. This technology includes transporting tools, working cloth, personal protective equipment, and pesticide-proof Clothing. Anthropometric study of Korean farmers was also carried out to support the development of equipment and clothes. In long terms, it needs to precaution activity from risk factors by using cultivation modification and working improvement. Although working condition gets better and workload reduce by this technology, it required customized safety technology in each crops - rice cultivation, horticulture and livestock farming. And farmers should use the assistive technology and participate voluntarily in ergonomic intervention program.

Development of W/O/W Multiple Emulsion Formulation Containing Burkholderia gladioli

  • KIM, HWA-JIN;CHO, YOUNG-HEE;BAE, EUN-KYUNG;SHIN, TAEK-SU;CHOI, SUNG-WON;CHOI, KEE-HYUN;PARK, JI-YONG
    • Journal of Microbiology and Biotechnology
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    • v.15 no.1
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    • pp.29-34
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    • 2005
  • W/O/W (water-in-oil-in-water) type multiple emulsion was applied to improve the storage stability of an antagonistic microorganism, Burkholderia gladioli. Encapsulation of microorganism into a W/O/W emulsion was conducted by using a two-step emulsification method. W/O/W emulsion was prepared by the incorporation of B. gladioli into rapeseed oil and the addition of polyglycerin polyriconolate (PGPR) and castor oil polyoxyethylene (COG 25) as the primary and secondary emulsifier, respectively. Microcrystalline cellulose was used as an emulsion stabilizer. To evaluate the usefulness of W/O/W emulsion formulation as a microbial pesticide for controlling the bacterial wilt pathogen (Ralstonia solanacearum), the storage stability and antagonistic activity of emulsion formulation were tested in vitro. The storage stability test revealed that the viability of formulated cells in emulsion was higher than that of unformulated cells in culture broth. At $4^{\circ}C$, the viabilities of formulated cells and unformulated cells at the end of 20 weeks decreased to about 2 and 5 log cycles, respectively. At $37^{\circ}C$, the viability of formulated cells decreased to only 2 log cycles at the end of storage. On the other hand, the viable cells in culture broth were not detected after 13 weeks. In activity test, formulated cells in emulsion were more effective in inhibiting the growth of pathogen than unformulated cells in culture broth. Unformulated cells completely lost their antagonistic activity during storage under similar conditions. The W/O/W multiple emulsion formulation was shown to be useful as the novel liquid formulation for biological control.

Degradation Patterns of BPMC and Carbofuran in Flooded Soils (담수토양(湛水土壤)중 BPMC 및 Carbofuran의 분해특성(分解特性))

  • Park, Chang-Kyu;Je, Yeon-Tae
    • Korean Journal of Environmental Agriculture
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    • v.2 no.2
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    • pp.65-72
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    • 1983
  • The effects of soil factors, pesticide application levels and environmental factors on the degradation of two carbamate insecticides, BPMC and carbofuran, in soil were studied under flooded and non-flooded conditions. The degradation patterns of BPMC or carbofuran were similar in flooded and non-flooded soils. It was also found that carbofuran was more persistent than BPMC in the test soil. Regards to organic contents of soils, high contents of organic matter reduced degradation of either BPMC or carbofuran and high soil pH accelerated degradation of both insecticides. Soil microorganisms seem to play an important role in the degradation of BPMC and carbofuran. Both chemical and biological degradations appear to contribute transformation of BPMC and carbofuran in the flooded soil.

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Insecticidal and Repellent Activities of Crude Saponin from the Starfish Asterias Amurensis

  • Park, Hee-Yeon;Kim, Ji-Young;Kim, Hyun-Ju;Lee, Geon-Hwi;Park, Jin-Il;Lim, Chi-Won;Kim, Yeon-Kye;Yoon, Ho-Dong
    • Fisheries and Aquatic Sciences
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    • v.12 no.1
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    • pp.1-5
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    • 2009
  • Crude saponin, extracted from the starfish Asterias amurensis, was assessed for its capacity as a biological pesticide. As part of this analysis, its insecticidal and repellent activities, in addition to its acute and chronic toxicities were tested. In comparison with the control group, insecticidal activity of saponin against tobacco cutworm, Spodoptera litura, in kale, Brassica loeracea was low at 36.4%. Repellent activities of the extracted saponin against green peach aphid, Myzus persicae, and S. litura, on soybean leaf, Glycine max and kale were 65.6% and 35.0% at $1^{st}$ day, and 54.5% and 30.0% at $3^{rd}$ day, respectively. Acute and chronic toxicity analysis was carried out using acute immobilizatioin test and reproduction impairment test, respectively. The saponin had 48 h-$EC_{50}$ of $65.21{\mu}g/mL$. Twenty-one day accumulative reproduction after treatment was lower in Daphnia magna at $7{\mu}g/mL$ saponin (78 youngs), compared with the control group (129 youngs). These results indicate that the extracted saponin exhibited some toxicity and has potential as a repellent against insects.