• 제목/요약/키워드: biological control

검색결과 4,957건 처리시간 0.035초

비병원성 Fusarium oxysporum 구조를 이용한 시금치 시들음병의 생물학적 방제 (Biological Control of Fusarium Wilt of Spinach by Nonpathogenic Isolates of Fusarium oxysporum)

  • 신동범;죽원이명
    • 한국식물병리학회지
    • /
    • 제14권2호
    • /
    • pp.145-149
    • /
    • 1998
  • Four nonpathogenic isolates of Fusarium oxysporum isolated from spinach showed suppressive effect on the occurrence of the Fusarium wilt of spinach caused by F. oxysporum f. sp. sprinaciae, among which NF01 controlled the disease most effectively. And NF01 was not pathogenic to tomato, cucumber, radish and spinach. This isolate was further tested for the biological control of the disease. The isolate was not inhibitory to the growth of the pathogen on potato sucrose agar medium, however the Fusarium wilt disease occurred less by drenching spore suspension of the nonpathogenic isolate. The control effect of the isolate was higher at lower inoculum level of the pathogen than at the higher inoculum level, and in the pretreatments than the simultaneous treatment of the isolate with the pathogen inoculation. The nit mutants of the isolate were easily formed on chlorate containing media, and was reisolated selectively as nit mutant from infected soil and plants. The reisolation rate of the isolate as opposed to pathogen was high at preinoculated soil and plants relative to the simultaneous inoculation of the isolate with the pathogen.

  • PDF

하천의 인공구조물이 저서성 대형무척추동물 군집에 미치는 영향 (The Influence of Artificial Structures on Benthic Macroinvertebrate Communities in Streams)

  • 김봉성;심광섭;김선희;권오창;서을원;이종은
    • 한국환경과학회지
    • /
    • 제22권3호
    • /
    • pp.309-318
    • /
    • 2013
  • This study was conducted for determining the influence of artificial structures on benthic macroinvertebrate communities in stream. Sampling was taken at upper(pool), down(riffle) and control(riffle) from two check dams, two weirs, one agricultural reservoir, and one multipurpose dam in northern part of Gyeongsangbuk-do. The benthic macroinvertebrate communities of these structures were surveyed during 2009 to 2011. The simple habitat of benthic macroinvertebrates occurred at the upper sites due to pooling effects from artificial structures. Specifically, Check dam1, Jusanji, Imha dam showed very low biological attribute values compared to the down and control sites, which have greater difference in substrate characteristics. However, in the upper sites of Check dam2, Weir1 and Weir2, the difference of values of biological attributes was relatively smaller. Also, proportion of functional feeding groups and functional habit groups were relatively simpler at upper stream and the degree of community differences was greater between upper and down, control sites. Spearman's correlation between biological attributes and substrate characteristics, water quality parameters had significant correlations; particularly, the substrate characteristics were more significantly related. In conclusion, the pool caused by artificial structures had negative effects on benthic macroinvertebrate communities thus leading to simplified stream habitats at upper stream ecosystems.

키토산 겔을 이용한 포름알데하이드 차폐 시트 개발 (Development of Formaldehyde-shielding Chitosan-gel Sheet)

  • 김소연;김미소;탁상민;이지환;심소연;주은희;김성배;김창준
    • KSBB Journal
    • /
    • 제29권3호
    • /
    • pp.183-187
    • /
    • 2014
  • Sick-building syndrome occurs when indoor air is polluted with harmful volatile organic compounds such as formaldehyde which are contained in furniture or new building materials. In this study, formaldehyde-shielding chitosangel sheet was developed and its performance was evaluated. Chitosan and agar were dissolved in acetic acid solution. The optimal concentrations of chitosan, acetic acid and agar were 3, 3, and 2.5 %(w/w). Formaldehyde was spreaded on gypsum board and then wall paper was attached on it by using glue. When chitosan-gel sheet was attached on this control board, the amount of formaldehyde released from the board was decreased by 63% than in control board. On the other hand, decrease in formaldehyde releasing was only 32% when liquid solution of chitosan was spreaded on the control board. This result clearly indicates that chitosan-gel sheet removes formaldehyde more effectively than liqud solution of chitosan. Furthermore, this type of sheet is more applicable to new building than spraying type.

청소년의 위험행동 구조모형 구축 (Development of the Structural Model of Adolescent's Risk Behavior)

  • 박현숙;정선영
    • 대한간호학회지
    • /
    • 제41권3호
    • /
    • pp.364-373
    • /
    • 2011
  • Purpose: In this study the fitness of a path model for the relationship among biological risk disposition, sociocultural risk factors, self-control, parent-adolescent communication, and risk behavior in adolescents was examined. Methods: The participants were 387 adolescents. The data were analyzed with the PASW 18.0 and AMOS 18.0 programs. Results: Sociocultural risk factors, self-control, and parent-adolescent communication showed a direct effect on risk behavior for adolescents, while biological risk disposition and sociocultural risk factor showed an indirect effect on risk behavior for adolescents. The modified path model of adolescents' risk behavior was showed a good fit with the model ($X^2$/df=2.37, GFI=.95, AGFI=.92, RMSEA=.06 [.05

포도나무 줄기 혹병의 생물학적 방제 (Biological control of grapevine crown gall)

  • 정광진;심재섭;정봉구
    • 농약과학회지
    • /
    • 제2권2호
    • /
    • pp.97-101
    • /
    • 1998
  • 최근에 재배면적이 급격히 증가하고 있는 포도에 난방제 병해인 줄기혹병의 길항세균을 선발하여 생물학적 방제의 가능성을 조사한 결과 포도 줄기혹병 이병포장으로부터 채취한 토양에서 길항균 strain 27를 선발하여 in vitro 및 in vivo bioassay결과 biovar 1 병원균인 A. tumefaciens C58과 Ach5, biovar 2 병원균인 A. rhizogenes 13264 및 biovar 3 병원균인 A. vitis 등을 모두 억제시키는 효과를 보였으며, Strain 27의 세균학적 특성은 pAgK84와 비슷한 크기(46 Kb)의 plasmid를 갖고 있음을 확인하였다.

  • PDF

High Cell Density Culture of Anabaena variabilis with Controlled Light Intensity and Nutrient Supply

  • Yoon, Jong-Hyun;Shin, Jong-Hwan;Ahn, Eun-Kyung;Park, Tai-Hyun
    • Journal of Microbiology and Biotechnology
    • /
    • 제18권5호
    • /
    • pp.918-925
    • /
    • 2008
  • Controlling the light energy and major nutrients is important for high cell density culture of cyanobacterial cells. The growth phase of Anabaena variabilis can be divided into an exponential growth phase and a deceleration phase. In this study, the cell growth in the deceleration phase showed a linear growth pattern. Both the period of the exponential growth phase and the average cell growth rate in the deceleration phase increased by controlling the light intensity. To control the light intensity, the specific irradiation rate was maintained above $10\;{\mu}mol/s/g$ dry cell by increasing the incident light intensity stepwise. The final cell density increased by controlling the nutrient supply. For the control of the nutrient supply, nitrate, phosphate, and sulfate were intermittently added based on the growth yield, along with the combined control of light intensity and nutrient concentration. Under these control conditions, both final cell concentration and cell productivity increased, to 8.2 g/l and 1.9 g/l/day, respectively.

Fly Ash Application for Reduction of Acid Mine Drainage (AMD) as Runoff and Leachate Released from Mine Waste Disposal Sites

  • Oh, Se Jin;Moon, Sung Woo;Oh, Seung Min;Kim, Sung Chul;Ok, Yong Sik;Lee, Bup Yeol;Lee, Sang Hwan;Yang, Jae E.
    • 한국토양비료학회지
    • /
    • 제47권6호
    • /
    • pp.533-539
    • /
    • 2014
  • Mine wastes such as acid mine drainage (AMD) can cause the detrimental effects on surrounding environment, thereby eventually threatening human health. Main objective of this study was to evaluate the neutralizing effect of fly ash (FA) as a stabilizing material AMD. Field plot was constructed in a coal waste depot which has caused aluminium-whitening adjacent to the stream. Different mixing ratios of FA were applied on a top of the soil, and then the physicochemical properties of runoff and soil were monitored. Constructed plots were as following: control (mine waste only (W)), mine waste + 20% ($w\;w^{-1}$)of FA (WC20M), mine waste + 40% ($w\;w^{-1}$)of FA (WC40M), and WC40M dressed with a fresh soil at the top (WC40MD). Result showed that initial pH of runoff in control was 5.09 while that in WC40M (7.81) was significantly increased. For a plot treated with WC40M, the concentration of Al in runoff was decreased to $0.22mg\;L^{-1}$ compared to the W as the control ($4.85mg\;L^{-1}$). Moreover, the concentration of Fe was also decreased to less than half at the WC40M compared to the control. Application of FA can be useful for neutralizing AMD and possibly minimizing adverse effect of AMD in mining area.

The Effects of Controlling the Aphis gossypii Glover (Homoptera, Aphididae) on Cucumber of Entomopahtogenic Fungus, Beauveria bassiana

  • Kang, Min-Ah;Youn, Young-Nam
    • 농업과학연구
    • /
    • 제35권2호
    • /
    • pp.127-136
    • /
    • 2008
  • 곤충병원성 곰팡이인 Beauveria bassiana는 미생물 살충제로서 다양한 해충들을 방제하는데 사용해오고 있다. 최근에 B. bassiana TBI-1균주가 두점박이응애, 온실가루이에 살충효과가 있는 것으로 보고되었다. 생물학적 방제 인자인 B. bassiana TBI-1를 목화진딧물에도 어느 정도의 살충활성을 나타내었다. B. bassiana TBI-1 균주를 처리한 후 일주일 동안 평균 진딧물의 증가율은 0.21로 나타났으나 처리하지 않은 대조군에서는 0.24의 증가율로 약간 높게 나타나 보였다. 이러한 결과에서 백강균의 처리구에서 진딧물의 평가 증가율이 0.03 낮게 나타나는 것을 알 수 있었다. 또한 오이 잎에 B. bassiana TBI-1 균주에 의하여 붉은빛 곰팡이의 형태로 죽은 진딧물의 죽은 흔적을 육안으로 확인할 수 있었다. 적정농도로 B. bassiana TBI-1을 처리하면 목화진딧물의 개체군 증가율을 현저히 감소시킴으로 효과적으로 진딧물을 방제할 수 있을 것이다.

  • PDF

인삼 잿빛곰팡이병의 친환경방제를 위한 유기농업자재 선발 (Selection of Environmental Friendly Organic Agricultural Materials for Controlling Ginseng Gray Mold)

  • 김우식;김종성;박지성;안인;박경훈;김기홍
    • 한국약용작물학회지
    • /
    • 제23권6호
    • /
    • pp.473-479
    • /
    • 2015
  • Background : To control ginseng gray mold, farmers have mainly used inorganic chemical based fungicides. The recent emergence of fungicide resistance has reduced the effectiveness of such control methods. Such pesticides also carry additional problems, such as diffuse pollution. Methods and Results : Six treatments of organic agricultural materials were tested for control of ginseng gray mold, CAPW (Chrysophanic acid + Phytoncide + Wood vinegar), EmEWV (Emodin + Ethanol + Wood vinegar), CEWV (Curcumin + Eugenol + Wood vinegar), Bacillus subtilis, soybean oil and sulfur. The control effect for gray mold by a single application of the agrochemical fungicide industrial Fenhexamid wettable powder (WP) was 84.4%. The control effect by CAPW, EmEWV and CEWV varied between 52.7 - 64.9%. The control effect by B. subtilis, soybean oil, and sulfur were 32.9 - 59.2%. Conclusions : In the field tests, CAPW showed the highest control effects when used before, and at first stage of disease incidence, against ginseng gray mold.