• Journal of Microbiology and Biotechnology
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• v.27 no.8
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• pp.1513-1518
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• 2017

Anaerobic mono- and co-digestion of sewage sludge and food waste leachate (FWL) were performed by assessing methane production and characterizing microbial communities. Anaerobic digestion (AD) of waste activated sludge (WAS) alone produced the lowest methane ($281ml\;CH_4$), but an approximately 80% increase in methane production was achieved via co-digestion of WAS and FWL ($506ml\;CH_4$). There were less differences in the diversity of bacterial communities in anaerobic digesters, while archaeal (ARC) and bacterial (BAC) amounts reflected AD performance. Compared with the total ARC and BAC amounts in the mono-digestion of WAS, the ARC and BAC amounts increased two and three times, respectively, during co-digestion of FWL and WAS. In characterized archaeal communities, the dominant ratio of hydrogenotrophic methanogens in the mono-digestion of WAS approached nearly a 1:1 ratio of the two acetoclastic and hydrogenotrophic methanogens in the co-digestion of FWL and WAS. The ARC/BAC ratio in the digesters varied in the range of 5.9% to 9.1%, indicating a positive correlation with the methane production of AD.

• Journal of Korean Society on Water Environment
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• v.32 no.3
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• pp.297-302
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• 2016

Microbial electrochemical technology (MET) has recently been studied to improve the efficiency of a traditional anaerobic digestion (AD). The purpose of this study was to investigate the impact of MET in the system when MET was combined with traditional AD (i.e., AD-MET). Electrodes used in the MET were Cu coated graphite electrodes. They were supplied with a voltage of 0.3 V. AD started to generate methane in 80 days. But AD-MET started to generate methane from the initial operation after the system started. It was observed that AD-MET reached steady state faster and produced higher methane yield than AD. During the steady state, the average daily methane productions in AD and AD-MET were 2.3L/d and 4.9L/d, respectively. Methane yields were 0.07-CH₄/g‧CODre in AD and 0.25L-CH₄/g‧CODre in AD-MET. In AD-MET, the production rates of total volatile fatty acids (TVFAs) and soluble chemical oxygen demand (SCOD) were 0.12 mg TVFAs/mg VS‧d and 0.35 mg SCOD/mg VS‧d, respectively. They were significantly (p < 0.05) higher than those in AD. However, the concentrations of residual TVFAs in both systems were not significantly (p > 0.05) different from each other, confirming that methane conversion in AD-MET was greater than that in AD.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.4
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• pp.327-334
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• 2016

In order to compare phosphorus (P) behavior of livestock manure compost (LMC) and chemical fertilizer (CF) applied to the sea-reclaimed land soils, incubation experiments were conducted for five weeks. Four soils differing textural classes, sandy loam and clay loam, and electric conductivity (EC) value, high and low, were applied with CF and LMC. LMC was applied at the level of 0, 1, 2, and 3% on the soil weight basis and CF was applied at the same levels of P as LMC. The results showed that increase of P application rate of CF and LMC led to linearly increase available $P_2O_5$ and $0.01M\;CaCl_2$ extractable P contents regardless of soil texture and EC. However, 0.01M $CaCl_2$ extractable P from soil applied with CF was significantly higher than with LMC. Correlation analysis between $0.01M-CaCl_2$ extractable P and fractionated P by different extraction methods showed that $0.01M-CaCl_2$ extractable P positively correlated with KCl-P (soluble and exchangeable P) and HCl-P (Ca and Mg bound P). However, NaOH-P (Fe and Al bound P and organic P) and residual P was adverse. The amount of NaOH-P significantly influenced to the amount of 0.01M $CaCl_2$ extractable P of CF and LMC in the soils. The application of LMC at sandy loam soil could be carried out in the consideration of nutrient leaching and crop uptake.

• Transactions of the Korean hydrogen and new energy society
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• v.26 no.3
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• pp.221-226
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• 2015

Ru catalysts supported on $CeO_2$ were synthesized by an impregnation method and characterized by numerous analytical techniques including X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), transmission electron microscopy (TEM), and scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS). Upon utilization of these catalysts for methane dry reforming with a $CH_4/CO_2$ ratio of 1:1 at different temperatures ranging from 550 to $750^{\circ}C$, the $Ru/CeO_2$ catalysts have shown to be active. In particular, Ru(0.55wt%) supported on $CeO_2$ (1) prepared by a hydrothermal method exhibited excellent activity with the conversion of > 75% at $750^{\circ}C$. In addition, the catalyst also proved to be highly stable for at least 47 h without catalyst deactivation under the dry reforming conditions.

• New & Renewable Energy
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• v.4 no.4
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• pp.56-64
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• 2008

Anaerobic biodegradability (AB), which can be determined with the ultimate methane yield by the decomposition of organic materials, is one of the important parameters for the design and the operation of anaerobic digestion plant. In this study, Biochemical methane potential (BMP) test has been carried out to evaluate the methane yields of animal manures such as pig and cattle slurries, and different forage crops cultivated at the reclaimed tideland such as maize, sorghum, barley, rye, Italian ryegrass (IRG), rape, rush, and waste sludge produced from slaughterhouse wastewater treatment plant (SSWTP). In the ultimate methane yield and biodegradability of animal manure, those of pig slurry were 345 $mlCH_4/gVS_{fed}$ and 44.7% higher than 247 $mlCH_4/gVS_{fed}$ and 46.4% of cattle slurry (Cat. 2). The ultimate methane yield and biodegradability of spike-crop rye (Rye 1) were 442.36 $mlCH_4/gVS_{fed}$ and 86.5% the highest among different forage crops, those of the other forage crops ranged from 306.6 to 379 $mlCH_4/gVS_{fed}$ of methane yield with the AB having the range of about 60 to 77%. Therefore the forage crops could be used as a good substrate to increase the methane production and to improve the biodegradability in anaerobic co-digestion together with animal manure.

• Journal of Microbiology and Biotechnology
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• v.9 no.6
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• pp.737-743
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• 1999

A conventional thermophilic anaerobic digester was converted into a thermophilic high-rate hybrid anaerobic reactor (THAR) for treating distillery wastewater. The THAR has been operating successfully since May 1995 at a loading rate of 5.45 to $11.52{\;}kg/\textrm{m}^3/d$ (maximum of 15.02). The THAR has demonstrated a soluble Chemical Oxygen Demand (sCOD) removal efficiency of 85 to 91% and a total COD (tCOD) removal efficiency of as much as 72 to 84%. Product gas had a methane content of 59 to 68%. The tCOD removal rates were 4.31 to 5.43, 6.26 to 6.89, and 9.03 to $9.78kg{\;}tCOD/\textrm{m}^3/d$ for tapioca, com, and naked-barley wastewater, respectively. The sCOD removal rates ranged from 3.75 to 4.79,3.28 to 4.89, and 5.57 to 6.21kg $sCOD/\textrm{m}^3/d$ for tapioca, com, and naked-barley wastewater, respectively. There were unknown substances in a naked-barley distillery wastewater that were identified as being toxic for microorganisms. However, the THAR treated naked-barley wastewater continuously for 26 days, operating at an average tCOD loading of $11.08{\;}kg/\textrm{m}^3/d$without any signs of deterioration in either COD removal efficiency or gas production rate. During this period, the average removal efficiencies of tCOD and sCOD were 84% and 91%, respectively, and the gas production rate averaged 6.61 to $7.57{\;}\textrm{m}^3/\textrm{m}^3$ reactor/d which produced 0.57 to $0.69{\;}\textrm{m}^3{\;}biogas/kg{\;}tCOD_{rem}$. From tapioca and com wastewater, the reactor showed an average gas production rate of 3.18 to 3.46 and 4.91 to $5.22{\;}\textrm{m}^3/\textrm{m}^3$ reactor/d which produced 0.53 to 0.69 and 0.62 to $0.71{\;}\textrm{m}^3/kg{\;}tCOD_{rem}$, respectively.

• Membrane Journal
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• v.30 no.5
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• pp.326-332
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• 2020

In this study, polymer-fluorinated silica composite hollow fiber membranes were fabricated and applied to a membrane contactor for the recovery of methane dissolved in the anaerobic effluent. To prepare the composite membranes, porous hollow fiber substrates were fabricated with Ultem^®, a commercial polyetherimide (PEI). Subsequently, fluorinated silica particles were synthesized and coated on the surface via strong covalent bonding. Due to the high porosity, our membrane showed a CH₄ flux of 8.25 × 10^-5 ㎤ (STP)/㎠·s at the liquid velocity of 0.03 m/s which is much higher that that of commercial polypropylene membrane designed for degassing processes. This is attributed to our membrane's high porosity as well as a superior surface hydrophobicity (120~122°) resulted from the coating with fluorinated silica nanoparticles.

• Journal of environmental and Sanitary engineering
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• v.23 no.1
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• pp.49-56
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• 2008

Foodwaste will be able to treat is 13,000 ton/day. $18{\times}10^{13}$ won/yr are wasted with the foodwaste treatment. As a result, the initial working condition. These are given as follows : On the 1st. week, the foodwaste of the 130 ton/week(digest sludge : input foodwaste = 20 : 1) is being put into a DRANCO system by the space of 2 day. On the 2nd week, the food waste of the 130 ton/week(5 day) is being put into a DRANCO system by the space of 2 day. On the 3rd week, the foodwaste of the 130 ton/week is being put into a DRANCO system by the space of 3day. On the 4th week, the foodwaste of the 350 ton/week is being put into a DRANCO system. After that time, increase the quantity than last week is 10%. Under steady-state working condition, the methane content of the biogas is more then 55%. The $NH_3-N$ content of the digest sludge is under 3,500ppm. The VFA content of the digest sludge is under 1,500ppm. The pH is more then 8. The TS content of the digest sludge is $18{\sim}22%$. The VS content of the digest sludge is under 65%.

• Journal of Korean Society on Water Environment
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• v.20 no.6
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• pp.583-588
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• 2004

This research examined the treatment efficiency and methane production rate in treating slurry-type swine waste using UASB (upflow anaerobic sludge blanket) reactor. The UASB reactor was operated at an organics volumetric loading rate (VLR) of $2.6-15.7kgCOD/m^3/day$. A stepwise increase of the VLR resulted in a temporary deterioration in the COD removal rate in UASB reactor but recovered quickly. The COD removal rate were 65-70% for VLR up to $5 kgCOD/m^3/day$. When organics VLR was $10kgCOD/m^3/day$, the COD removal rate decreased sharply and there was loss of 17.537g of the seeding biomass due to sludge washout. This result indicated that the UASB system cannot be adapted to more than $10kgCOD/m^3/day$ of VLR. As the organic load increased from 2.6 to $15.7kgCOD/m^3/d$, the biogas production rate varied from 3.2 to 10.8 L/d and the methane conversion rate of the organic matter varied from 0.30 to $0.23m^3CH_4/kg\;COD_{removed}$. The methane content showed the range of 70.1-81.5% during the experimental period. The volatile solids (VS) removal efficiency was similar at the low VLR (< $5 kgCOD/m^3/day$), but it decreased sharply at the high VLR (> $5 kgCOD/m^3/day$). The VS reduction rate was, moreover, large those of COD. The result shows that hydraulic retention time above 2 days is essential in case of treating wastewater containing 1% of solids.

• Korean Journal of Organic Agriculture
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• v.20 no.3
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• pp.327-343
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• 2012

In order to develop a high cellulolytic direct-fed microorganism (DFM) for ruminant productivity improvement, this study isolated cellulolytic bacteria from the rumen of Holstein dairy cows, and compared their cellulolytic abilities via DM degradability, gas production and cellulolytic enzyme activities. Twenty six bacteria were isolated from colonies grown in Dehority's artificial (DA) medium with 2% agar and cultured in DA medium containing filter paper at $39^{\circ}C$ for 24h. 16s rDNA gene sequencing of four strains from isolated bacteria showed that H8, H20 and H25 strains identified as Ruminococcus flavefaciens, and H23 strain identified as Fibrobacter succinogenes. H20 strain had higher degradability of filter paper compared with others during the incubation. H8 (R. flavefaciens), H20 (R. flavefaciens), H23 (F. succinogenes), H25 (R. flavefaciens) and RF (R. flavefaciens sijpesteijn, ATCC 19208) were cultured in DA medium with filter paper as a single carbon source for 0, 1, 2, 3, 4 and 6 days without shaking at $39^{\circ}C$, respectively. Dry matter degradability rates of H20, H23 and H25 were relatively higher than those of H8 and RF since 2 d incubation. The cumulative gas production of isolated cellulolytic bacteria increased with incubation time. At every incubation time, the gas production was highest in H20 strain. The activities of carboxymethylcellulase (CMCase) and Avicelase in the culture supernatant were significantly higher in H20 strain compared with others at every incubation time (p<0.05). Therefore, although further researches are required, the present results suggest that H20 strain could be a candidate of DFM in animal feed due to high cellulolytic ability.