• 상하수도학회지
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• 제37권6호
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• pp.325-334
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• 2023

Membrane bioreactor (MBR) provides the benefits on high effluent quality and construction cost without the secondary clarification. Despite of these advantages, fouling, which clogs the pore in membrane modules, affects the membrane life span and effluent quality. Studies on the laboratory scale MBR were focused on the control of particulate fouling, organic fouling and inorganic fouling. However, less studies were focused on the control of biofouling and microbial aspect of membrane. In the full scale operation, most MBR produces high effluent quality to meet the national permit of discharge regulation. In this study, the performance and microbial community analysis were investigated in two MBRs. As the results, the performance of organic removal, nitrogen removal, and phosphorus removal was similar both MBRs. Microbial community analysis, however, showed that Azonexus sp. and Propionivibrio sp. contributed to indirect fouling to cause the chemical cleaning in the DX MBR.

• 멤브레인
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• 제10권2호
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• pp.75-82
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• 2000

본 연구는 정수처리 공정에서 예상되는 오염물질에 대한 막의 투과성능과 오염현상을 알아보기 위하여 막의 깊이와 운전압력에 따른 압밀화 현상을 검토하였다. 막의 길이와 운전압력이 증가함에 따라 압밀화 현상도 증가하였다. MF막을 오염물질에서 고압(2.0 kg/$cm^2$)과 저압(0.5 kg/$cm^2$)으로 운전할 때, 고압 운전이 막힘현상(clogging)을 가속화 시켜 플럭스를 급격히 감소시키므로 저압 운전이 효과적이다. MF막을 Out-In방식으로 운전하였을 때, 막의 길이와 운전 압력에 관계없이 일정 시간 운전 후 비슷한 플럭스가 나타났다. 미생물 물질에 대한 막오염 현상을 파악하기 위해 중공사막을 강제 오염시킨 후 화학세정을 실시하였다. Biofouling된 막을 화학세정 하였을 때 산화능력이 우수한 $H_2O_2$와 NaOCl이 NaOH에 비해 살균능력과 탈착능력이 우수하다는 것을 알 수 있었다.

• 상하수도학회지
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• 제28권2호
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• pp.151-160
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• 2014

Capacitive deionization(CDI) has many advantages over other desalination technologies due to its low energy consumption, less environmental pollution and relative low fouling potential. The objectives of this study are evaluate the performance of CDI which can be used for dissolved salts removal from sewage. To identify ion selectivity of nitrate and phosphate in multiionic solutions and adsorption/desorption performance related to applied potential, a series of laboratory scale experiments were conducted using a CDI unit cell with activated carbon electrodes. The CDI process was able to achieve more than 75 % TDS and $NO_3{^-}$, $NH_4{^+}$ removals, while phosphate removal was 60.8 % and is inversely related in initial TDS and $HCO_3{^-}$ concentration. In continuous operation, increasing the inner cell pressure and reduction of TDS removal ability were investigated which are caused by inorganic scaling and biofouling. However a relative mild cleaning solution(5 % of citric acid for calcium scaling and 500 mg/L of NaOCl for organic fouling) restored the electrochemical adsorption capacity of the CDI unit to its initial level.

• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1908-1919
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• 2015

This work investigated the potential of curcumin (CCM) and (-)-epigallocatechin gallate (EGCG) to inhibit N-acyl homoserine lactone (AHL)-mediated biofilm formation in gram-negative bacteria from membrane bioreactor (MBR) activated sludge. The minimum inhibitory concentrations (MICs) of CCM alone against all the tested bacteria were 200-350 μg/ml, whereas those for EGCG were 300-600 μg/ml. Biofilm formation at one-half MICs indicated that CCM and EGCG alone respectively inhibited 52-68% and 59-78% of biofilm formation among all the tested bacteria. However, their combination resulted in 95-99% of biofilm reduction. Quorum sensing inhibition (QSI) assay with known biosensor strains demonstrated that CCM inhibited the expression of C4 and C6 homoserine lactones (HSLs)-mediated phenotypes, whereas EGCG inhibited C4, C6, and C10 HSLs-based phenotypes. The Center for Disease Control biofilm reactor containing a multispecies culture of nine bacteria with one-half MIC of CCM (150 μg/ml) and EGCG (275 μg/ml) showed 17 and 14 μg/cm² of extracellular polymeric substances (EPS) on polyvinylidene fluoride membrane surface, whereas their combination (100 μg/ml of each) exhibited much lower EPS content (3 μg/cm²). Confocal laser scanning microscopy observations also illustrated that the combination of compounds tremendously reduced the biofilm thickness. The combined effect of CCM with EGCG clearly reveals for the first time the enhanced inhibition of AHL-mediated biofilm formation in bacteria from activated sludge. Thus, such combined natural QSI approach could be used for the inhibition of membrane biofouling in MBRs treating wastewaters.

• Membrane and Water Treatment
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• 제8권1호
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• pp.51-71
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• 2017

Antimicrobial polyethersulfone ultrafiltration membranes containing zerovalent iron ($Fe^0$) and magnetite ($Fe_3O_4$) nanoparticles were synthesized via phase inversion method using polyethersulfone (PES) as membrane material and nano-iron as nanoparticle materials. Zerovalent iron nanoparticles (nZVI) were prepared by the reduction of iron ions with borohydride applying an inert atmosphere by using $N_2$ gases. The magnetite nanoparticles (nMag) were prepared via co-precipitation method by adding a base to an aqueous mixture of $Fe^{3+}$ and $Fe^{2+}$ salts. The synthesized nanoparticles were characterized by scanning electron microscopy, X-ray powder diffraction, and dynamic light scattering analysis. Moreover, the properties of the synthesized membranes were characterized by scanning electron microscopy energy dispersive X-ray spectroscopy and atomic force microscopy. The PES membranes containing the nZVI or nMag were examined for antimicrobial characteristics. Moreover, amount of iron run away from the PES composite membranes during the dead-end filtration were tested. The results showed that the permeation flux of the composite membranes was higher than the pristine PES membrane. The membranes containing nano-iron showed good antibacterial activity against gram-negative bacteria (Escherichia coli). The composite membranes can be successfully used for the domestic wastewater filtration to reduce membrane biofouling.

• 한국해양환경ㆍ에너지학회지
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• 제16권2호
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• pp.82-87
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• 2013

본 논문에서는 해수에 잠겨있는 물체표면의 물리적 성질과 생물오손현상 간의 상관관계를 관찰하기 위하여 생물오손에 미치는 소수성(hydrophobic) 표면의 효과에 대해 실험해석을 수행하였다. 시편으로서, 일반알루미늄, 소수성표면을 가진 알루미늄, 친수성(hydrophilic)표면을 가진 알루미늄 등 세 종류를 사용하였으며, 단, 소수성시편의 경우, AAO(Anodic Aluminum Oxide)기법으로 제작한 것과 HDFS(heptadecafluoro-1,1,2,2,-tetrahydrodecyltricholorosilane)코팅처리하여 제작한 것, 두 종류를 사용하였다. 세 종류, 네 개의 시편에서 확인된 생물오손정도는 중장기적인 면에서는 시편 간에 큰 차이가 없지만, 오손초기에는 괄목할 만한 차이가 관찰되었다. 생물 부착물의 두께가 소수성표면의 미세돌기 높이에 다다를 때 까지는 소수성표면의 오손지연효과가 현저하게 나타나나, 일단 이를 초과하면 소수성표면의 오손방지효과는 소멸됨을 확인하였다.

• 환경생물
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• 제17권3호
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• pp.315-319
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• 1999

갈파래의 포자가 부착한 Agar와 Agarose코팅필름은 agar, agarose 또는 메탄놀의 농도에 따라 다양한 표면의 성질을 나타냈다. Sigma agarose 코팅필름에서 가장 많은 수의 포자 부착이 일어났다. Bacto agar과 그외 agarose으로 코팅한 필름에 부착한 포자는 Sigma agarose으로 코팅한 필름보다 12∼36배나 적었다. 2.5%와 5％ 의 SeaKem agarose와 Bacto agar 코팅필름의 비교에서 두 개의 농도 차이가 있는 반면에, 2.5%와 5%의 NuSieve와 Sigma agarose 코팅필름의 비교에서는 차이가 없었다. 5％ 의 Bacto agar와 4％ 의 메탄놀을 포함한 5% Bacto agar 비교, 5％ NuSieve와 4％ 메탄놀을 포함한 5% NuSieve agarose비교에서만 차이를 보였다. 본 연구의 전반적인 결과는 agar와 agarose로 코팅한 필름에서 포자 부착의 차이가 있음을 알 수 있었다. 본연구 결과는 착생생물에 대한 반착생을 검색할 수 있는 기초가 되어질 것이다.

• Journal of Microbiology and Biotechnology
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• 제24권11호
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• pp.1574-1582
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• 2014

Bacteria recognize changes in their population density by sensing the concentration of signal molecules, N-acyl-homoserine lactones (AHLs). AHL-mediated quorum sensing (QS) plays a key role in biofilm formation, so the interference of QS, referred to as quorum quenching (QQ), has received a great deal of attention. A QQ strategy can be applied to membrane bioreactors (MBRs) for advanced wastewater treatment to control biofouling. To isolate QQ bacteria that can inhibit biofilm formation, we isolated diverse AHL-degrading bacteria from a laboratory-scale MBR and sludge from real wastewater treatment plants. A total of 225 AHL-degrading bacteria were isolated from the sludge sample by enrichment culture. Afipia sp., Acinetobacter sp. and Streptococcus sp. strains produced the intracellular QQ enzyme, whereas Pseudomonas sp., Micrococcus sp. and Staphylococcus sp. produced the extracellular QQ enzyme. In case of Microbacterium sp. and Rhodococcus sp., AHL-degrading activities were detected in the whole-cell assay and Rhodococcus sp. showed AHL-degrading activity in cell-free lysate as well. There has been no report for AHL-degrading capability in the case of Streptococcus sp. and Afipia sp. strains. Finally, inhibition of biofilm formation by isolated QQ bacteria or enzymes was observed on glass slides and 96-well microtiter plates using crystal violet staining. QQ strains or enzymes not only inhibited initial biofilm development but also reduced established biofilms.

• 한국환경과학회지
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• 제17권12호
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• pp.1353-1361
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• 2008

Quality control of Argo(Array for Real-time Geostrophic Oceanography) data is crucial by reason that salinity measurements are liable to experience some drift and offset due to biofouling, contamination of sensor and wash-out of biocide. The automated Argo real-time quality control has a limit of sorting data quality, so that WJO program is adopted as standardized method of Argo delayed mode quality control (DMQc) in the world that is a precise quality control method. We conducted DMQC on pressure, temperature and salinity measured by Argo floats in the Pacific Ocean including expert evaluation. Particularly, salinity data were corrected using WJO program. 4 salinity profiles of Argo delayed mode were compared with nearby in situ CTD data and other Argo data in deep layer where oceanographic conditions are stable in time and space. The differences of both salinities were lower than target accuracy of Argo. As compared with the difference of salinities before DMQC, those after DMQC decreased by 60-80 percent. Quality of delayed mode salinity data seemed to be improved correcting salinity data suggested by WJO program.

• ALGAE
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• 제28권2호
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• pp.173-183
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• 2013

Sodium hypochlorite (NaOCl) is widely used to disinfect seawater in power plant cooling systems in order to reduce biofouling, and in ballast water treatment systems to prevent transport of exotic marine species. While the toxicity of NaOCl is expected to increase by ongoing ocean acidification, and many experimental studies have shown how algal calcification, photosynthesis and growth respond to ocean acidification, no studies have investigated the relationship between NaOCl toxicity and increased $CO_2$. Therefore, we investigated whether the impacts of NaOCl on survival, chlorophyll a (Chl-a), and effective quantum yield in three marine phytoplankton belonging to different taxonomic classes are increased under high $CO_2$ levels. Our results show that all biological parameters of the three species decreased under increasing NaOCl concentration, but increasing $CO_2$ concentration alone (from 450 to 715 ${\mu}atm$) had no effect on any of these parameters in the organisms. However, due to the synergistic effects between NaOCl and $CO_2$, the survival and Chl-a content in two of the species, Thalassiosira eccentrica and Heterosigma akashiwo, were significantly reduced under high $CO_2$ when NaOCl was also elevated. The results show that combined exposure to high $CO_2$ and NaOCl results in increasing toxicity of NaOCl in some marine phytoplankton. Consequently, greater caution with use of NaOCl will be required, as its use is widespread in coastal waters.