• Title/Summary/Keyword: biofouling

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Isolation and Characterization of Biofouling Bacteria in Ultra-high Purity Water Used in the Semiconductor Manufacturing Process

  • Kim, In-Seop;Lee, Kye-Joon
    • Journal of Microbiology and Biotechnology
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    • v.10 no.4
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    • pp.554-558
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    • 2000
  • Bacteria were isolated and identified from an advanced high-purity water system that supplies ultra-high purity water (UHPW) for 16-megabyte DRAM semiconductor manufacturing. Scanning electron microscopic and microbiological observations revealed that the primary source of the bacteria isolated from the UHPW was detached cells from biofilms developed on the pipe wall through which the UHPW, a man-made and extremely nutrient poor environment, was passing. About 63-65% of the bacteria isolated from the UHPW and the pipe wall were Gram-positive, whereas only 10% of the bacteria isolated from the feed water were Gram-positive. The of Gram-positive bacteria and seven genera of Gram-negative bacteria. Strains of the UHPW bacteria effectively adhered to and formed a biofilm on the surface of polyvinyl chloride (PVC) pipe.

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Image Analysis Method for the Performance Evaluation of Marine Antifouling Coatings (화상 분석을 통한 선박 방오도료의 성능 평가)

  • Park, Hyun;Chun, Ho Hwan;Lee, Inwon
    • Journal of the Korean Society of Visualization
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    • v.11 no.2
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    • pp.18-26
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    • 2013
  • An accurate and reliable performance evaluation technique is indispensable for the development of marine antifouling coatings. The existing standard practice is however, based on the visual observation of biofouling settlement area, which is prone to the subjective judgment of the inspector. In spite of the above mentioned importance, a systematic and objective fouling evaluation technique has not yet been introduced. In this study, a novel quantitative antifouling performance evaluation method for marine antifouling paints is devised based on the image analysis of panel immersion test results. The present image analysis method is to quantify settlement area for each fouling category by distinctive color. The fouling categories are set as unfouled, biofilm, green algae, brown algae, calcareous animal and spongy animal with specific HSL (Hue, Saturation, Lightness) color ranges. In order to assess the effectiveness of the proposed method, static immersion tests for three antifouling coatings were undertaken for two years.

Bioinspired Nanoengineering of Multifunctional Superhydrophobic Surfaces

  • Choi, Chang-Hwan
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2015.11a
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    • pp.102-133
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    • 2015
  • Nature, such as plants, insects, and marine animals, uses micro/nano-textured surfaces in their components (e.g., leaves, wings, eyes, legs, and skins) for multiple purposes, such as water-repellency, anti-adhesiveness, and self-cleanness. Such multifunctional surface properties are attributed to three-dimensional surface structures with modulated surface wettability. Especially, hydrophobic surface structures create a composite interface with liquid by retaining air between the structures, minimizing the contact area with liquid. Such non-wetting surface property, so-called superhydrophobicity, can offer numerous application potentials, such as hydrodynamic drag reduction, anti-biofouling, anti-corrosion, anti-fogging, anti-frosting, and anti-icing. Over the last couple of decades, we have witnessed a significant advancement in the understanding of surface superhydrophobicity as well as the design, fabrication, and applications of superhydrophobic coatings/surfaces/materials. In this talk, the designs, fabrications, and applications of superhydrophobic surfaces for multifunctionalities will be presented, including hydrodynamic friction reduction, anti-biofouling, anti-corrosion, and anti-icing.

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Effect of Feed Monochloramination on Performance of RO Membranes (NH2Cl 사용으로 인한 RO 막의 성능 향상)

  • Hong, Seungkwan;Reiss, Robert;Taylor, James S.;Beverly, Sharon
    • Journal of Korean Society of Water and Wastewater
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    • v.19 no.3
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    • pp.312-317
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    • 2005
  • The 15 month pilot study was performed to investigate the effect of pretreatment by monochloramine ($NH_2Cl$) on the performance of RO membranes made of cellulose acetate (CA) and polyamide (PA). Both RO membranes experienced severe biological fouling without any pretreatment during the treatment of highly organic surface water in Florida, USA. Feed monochloramination at 5 mg/L significantly minimized productivity loss by effective control of biofouling. The CA membrane did not show any structural damages by monochloramine, while the PA membrane suffered from a gradual loss of membrane integrity by chlorine oxidation, which was characterized as an increase in productivity and a decrease in selectivity. The degradation of PA membrane increased with increasing monochloramine dose.

Effect of the Shape and Size of Quorum-Quenching Media on Biofouling Control in Membrane Bioreactors for Wastewater TreatmentS

  • Lee, Seonki;Lee, Sang Hyun;Lee, Kibaek;Kwon, Hyeokpil;Nahm, Chang Hyun;Lee, Chung-Hak;Park, Pyung-Kyu;Choo, Kwang-Ho;Lee, Jung-Kee;Oh, Hyun-Suk
    • Journal of Microbiology and Biotechnology
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    • v.26 no.10
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    • pp.1746-1754
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    • 2016
  • Recently, spherical beads entrapping quorum quenching (QQ) bacteria have been reported as effective moving QQ-media for biofouling control in MBRs for wastewater treatment owing to their combined effects of biological (i.e., quorum quenching) and physical washing. Taking into account both the mass transfer of signal molecules through the QQ-medium and collision efficiencies of the QQ-medium against the filtration membranes in a bioreactor, a cylindrical medium (QQ-cylinder) was developed as a new shape of moving QQ-medium. The QQ-cylinders were compared with previous QQ-beads in terms of the QQ activity and the physical washing effect under identical loading volumes of each medium in batch tests. It was found that the QQ activity of a QQ-medium was highly dependent on its specific surface area, regardless of the shape of the medium. In contrast, the physical washing effect of a QQ-medium was greatly affected by its geometric structure. The enhanced anti-biofouling property of the QQ-cylinders relative to QQ-beads was confirmed in a continuous laboratory-scale MBR with a flat-sheet membrane module.

A Study on the Hydrodynamic Effect of Biofouling on Marine Propeller (선박 프로펠러 표면의 생물부착물이 프로펠러 유체역학적 성능에 미치는 영향에 관한 연구)

  • Seo, Kwang-Cheol;Atlar, Mehmet;Goo, Bonguk
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.22 no.1
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    • pp.123-128
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    • 2016
  • The effect of propeller surface roughness condition on ship performance is very significant even the influence of fouling on propeller performance is not well established compared to biofouling on the hull surface. In present study, predictions of open water efficiency of propeller are made for three different fouling conditions, and its application is given for the 7m full-scale propeller of a medium-size tanker in open water condition. The numerical predictions of propeller efficiency loss due to fouling are based on the results from laboratory-scale drag measurements and boundary layer similarity law analysis presented in Schultz (2007) together with an in-house unsteady lifting surface code which is an appropriate tool to predict the effect of propeller surface roughness on propeller performance. The results of this study indicate that the subject propeller with the small calcareous fouling ($k_s=0.001$) can lead to as high as 15 % loss at the propeller operating condition (J=0.5) and the loss of propeller efficiency due to fouling should be evaluated while the ship is operating.

Adhesive Polyethylene Glycol Coatings for Low Biofouling Copper-Zinc Alloy Substrates (황동 표면의 생물 부착 억제를 위한 접착성 폴리에틸렌글라이콜 코팅)

  • Sang-woo Lee;Hyun Ho Shin;Seokjun Kwon;Ji Hyun Ryu
    • Journal of Adhesion and Interface
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    • v.24 no.3
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    • pp.105-111
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    • 2023
  • Recently, there has been a growing interest in low biofouling coatings for various industrial applications including precious metal and jewelry applications. Contaminations including cells and bacteria of the metallic substrates (i.e., accessories, earring, and piercings) may irritate the contacted tissue surfaces or induce an abnormal reaction. In this study, catechol-conjugated polyethylene glycol (PEG-C) was synthesized as low bio-fouling coating materials inspired by mussel-adhesion. PEG-C-coated copper-zinc alloy surfaces showed excellent cell viability and significant inhibitions of protein and cell adhesions to metal surfaces. Thus, PEG-C coating methods and PEG-C-coated metallic substrates can be usefully exploited for versatile industrial applications, particularly for precious metal and jewelry industries.

Characterization of Quorum-Quenching Bacteria Isolated from Biofouled Membrane Used in Reverse Osmosis Process (Biofouling이 일어난 역삼투막에서 분리한 쿼럼 저해 세균의 특성)

  • Moon, Sooyoung;Huang, Xinxin;Choi, Sung-Chan;Oh, Young-Sook
    • Korean Journal of Microbiology
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    • v.50 no.2
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    • pp.128-136
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    • 2014
  • Acyl homoserine lactone (AHL) lactonase has been proved to be the AHL-degrading enzyme with the highest substrate specificity for AHL molecules and has shown a considerable potential as low-cost and efficient quorum quenching (QQ) technique. However, few studies focused on its inhibitory effect on biofilm formation which is also a quorum sensing (QS)-regulated phenomenon. In this study, QQ activity of six isolates from biofouled reverse osmosis membranes was studied using Chromobacterium violaceum CV026 and Agrobacterium tumefaciens NTL4 as biosensors under various conditions. All of the isolates belonged to the genus Bacillus and showed QQ activity regardless of the acyl chain length or substitution of AHL molecule. The isolates were capable of significantly inhibiting biofilm formation (46.7-58.3%) by Pseudomonas aeruginosa PAO1 and produced heat-sensitive extracellular QQ substances. The LC-MS analysis of the QQ activity of a selected isolate, RO1S-5, revealed the degradation of N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12 AHL) and the production of corresponding acyl homoserine (3-oxo-C12-HS), which indicated the activity of AHL lactonase. The broad AHL substrate range and high substrate specificity suggested that the isolate would be useful for the control of biofilm-related pathogenesis and biofouling in industrial processes.

Improving Microalgal Biomass Productivity and Preventing Biofouling in Floating Marine Photobioreactors via Sulfonation of Selectively Permeable Membranes (부유형 해양 광생물반응기의 선택적 투과막의 술폰화 반응을 통한 Biofouling 억제 및 미세조류 생산성 향상)

  • Kim, Kwangmin;Lee, Yunwoo;Kim, Z-Hun;Park, Hanwool;Jung, Injae;Park, Jaehoon;Lim, Sang-Min;Lee, Choul-Gyun
    • Journal of Marine Bioscience and Biotechnology
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    • v.9 no.1
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    • pp.14-21
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    • 2017
  • The purpose of this study was to inhibit biofouling on a selectively permeable membrane (SPM) and increase biomass productivity in marine photobioreactors (PBRs) for microalgal cultivation by chemical treatment. Surfaces of a SPM, composed of polyethylene terephthalate (PET), was sulfonated to decrease hydrophobicity through attaching negatively charged sulfonic groups. Reaction time of sulfonation was varied from 0 min to 60 min. As the reaction time increased, the water contact angle value of SPM surface was decreased from $75.5^{\circ}$ to $44.5^{\circ}$, indicating decrease of surface hydrophobicity. Furthermore, the water permeability of sulfonated SPM was increased from $5.42mL/m^2/s$ to $10.58mL/m^2/s$, which reflects higher nutrients transfer rates through the membranes, due to decreased hydrophobicity. When cultivating Tetraselmis sp. using 100-mL floating PBRs with sulfonated SPMs, biomass productivity was improved by 34% compared with the control group (non-reacted SPMs). In addition, scanning electron microscopic observation of SPMs used for cultivation clearly revealed lower degree of cell attachment on the sulfonated SPMs. These results suggest that sulfornation of a PET SPM could improve microalgal biomass productivity by increasing nutrients transfer rates and inhibiting biofouling by algal cells.