• Title/Summary/Keyword: bioenergy crop

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Optimization of Cellulolytic Enzyme Production for newly isolated Bacillus sp. H9-1 from Herbivore Feces (초식동물 배설물로부터 분리한 Bacillus sp. H9-1의 섬유소 분해효소생산 최적화)

  • Yoon, Young Mi;An, Gi Hong;Kim, Jung Kon;Cha, Young-Lok;Park, Yu Ri;Ahn, Jong-Woong;Moon, Youn-Ho;Ahn, Seung-Hyun;Koo, Bon-Cheol;Park, Kwang-Geun
    • KSBB Journal
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    • v.28 no.1
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    • pp.42-47
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    • 2013
  • This study was performed to find cellulolytic strain of enzymatic saccharification for bioethanol production. Cellulolytic strains were isolated from 59 different feces of herbivores from Seoul Grand Park located in Gwacheon Gyeonggi-Do. The celluloytic strain was selected by congo red staining and DNS method. Among the isolated strains, H9-1 strain isolated from the feces of rabbit has the highest CMCase activity. H9-1 strain was identified as Bacillus sp. based on 16S rDNA gene sequencing. The optimal conditions for CMCase activity by Bacillus sp. H9-1 were at $40^{\circ}C$ and at initial pH 8.

Evaluation of Fiber Content According to the Cultivation Period of New Sweetpotato Varieties

  • Won Park;Mi Nam Chung;Koan Sik Woo;Hyeong-Un Lee;Tae Hwa Kim;Su Jung Kim;Kyo Hwui Lee;Sang Sik Nam
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.54-54
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    • 2022
  • Recently, as one of the major problems in the quality of sweetpotato, occurrence of thin and long fibrous tissues in storage root acts as a negative factor when consumers eat sweetpotato. In this study, the fiber content was compared according to the cultivation period in storage roots of 'Sodammi' and 'Hopungmi', which were newly bred and developed, and in that of 'Hogammi', which contains a lot of fibrous tissues. To isolate of fiber from storage root, the Association Official Analytical Chemists (AOAC) method was applied for quantifying fiber present in storage root of sweetpotato. The fiber contents isolated by this method is calculated by converting the weight of the storage root. The fiber content was measured every 20 days from 60 to 120 days after planting. As a result of this study, the lowest amount of fiber was 'Hopungmi' (70~140 mg/100 g), and the highest amount of fiber was observed in 'Hogammi' (115~223 mg/100 g). 'Sodammi' showed an intermediate level (104~149 mg/100 g) between the fiber content of 'Hopungmi' and 'Hogammi'. The fiber contents of 'Hopungmi' was 39% lower than that of 'Hogammi'. As the increased cultivation periods, the fiber contents showed a tendency to decrease. In the future research, the length, thickness, and fiber contents will be investigated to compare the degree of taste inhibition.

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Changes of Morphological and Growth Characteristics Collected Miscanthus Germplasm in Korea (국내 억새 유전자원 수집 후 형태 및 생육 특성 변화)

  • Song, Yeon-Sang;Lee, Ji-Eun;Moon, Youn-Ho;Yu, Gyeong-Dan;Choi, In-Seong;Cha, Young-Lok;Kim, Kwang-Soo
    • Weed & Turfgrass Science
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    • v.7 no.1
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    • pp.22-34
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    • 2018
  • Miscanthus has been considered as the most promising bioenergy crop for lignocellulosic biomass production. In Korea, M. sacchariflorus and M. sinensis can be found easily in all regions. It is a great advantage to utilize as important species with respect to genetic and cross-breeding programs materials for creation of novel hybrids. For successful breeding programs, it is important to precisely understand the variability of morphological and growth characteristics among Miscanthus species as breeding parent materials. In this study, morphological and growth characteristics were observed in 960 germplasms of two Miscanthus species (M. sacchariflorus and M. sinensis) for growing seasons over three years. Due to the inherent characteristics of these species, the germplasm of M. sacchariflorus among the collected germplasm were reduced in plant height than in the collection area. In M. sinensis, the plant height of germplasm collected mainly from Jeju-do increased more than those collected from collection area. Sixty-one of the collected 960 germplasms were selected and investigated to the morphological characteristics. Based on the investigated morphological data, the phylogenic tree was developed. As the results, it was confirmed that there exist germplasm in which the characteristics of M. sacchariflorus and M. sinensis are mixed. This study of Miscanthus may provide an important information in order to expedite the introduction as breeding materials for creation of new hybrid.