• Title/Summary/Keyword: bioenergy

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Optimization of Cellulolytic Enzyme Production for newly isolated Bacillus sp. H9-1 from Herbivore Feces (초식동물 배설물로부터 분리한 Bacillus sp. H9-1의 섬유소 분해효소생산 최적화)

  • Yoon, Young Mi;An, Gi Hong;Kim, Jung Kon;Cha, Young-Lok;Park, Yu Ri;Ahn, Jong-Woong;Moon, Youn-Ho;Ahn, Seung-Hyun;Koo, Bon-Cheol;Park, Kwang-Geun
    • KSBB Journal
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    • v.28 no.1
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    • pp.42-47
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    • 2013
  • This study was performed to find cellulolytic strain of enzymatic saccharification for bioethanol production. Cellulolytic strains were isolated from 59 different feces of herbivores from Seoul Grand Park located in Gwacheon Gyeonggi-Do. The celluloytic strain was selected by congo red staining and DNS method. Among the isolated strains, H9-1 strain isolated from the feces of rabbit has the highest CMCase activity. H9-1 strain was identified as Bacillus sp. based on 16S rDNA gene sequencing. The optimal conditions for CMCase activity by Bacillus sp. H9-1 were at $40^{\circ}C$ and at initial pH 8.