• 폴리머
• /
• 제39권3호
• /
• pp.493-498
• /
• 2015

피부재생을 위한 생체재료는 염증반응이 최소화되는 안정한 소재로 빠른 피부재생을 돕기 위해 우수한 생체활성과 생체친화성을 가져야 하며, 세포의 부착과 성장을 돕는 미세구조와 다공성이 있어야 한다. 본 연구에서는 새로운 콜라겐 원으로서의 축산부산물인 오리발을 사용하여 콜라겐을 추출하였고 이를 탈미네랄화된 골분demineralized bone powder, DBP), 돼지 소장점막하 조직(small intestinal submucosa, SIS)과 비교하기 위해 스펀지 형태로 제작하였다. 지지체의 물리, 화학적 특징은 SEM, FTIR을 통해 확인하였다. 세포를 파종하여 MTT를 통해 세포의 부착 및 증식률을 측정하였고, 전염증성 사이토카인의 발현도를 보기 위해 RT-PCR을 실시하였다. 또한 항산화 활성능력을 보기 위해 1,1-diphenyl-2-picrylhydrazyl(DPPH)를 측정하였다. 그 결과 오리발 콜라겐 지지체가 물리적 특성이 우수하고 생체적합성이며, 상처 치유제로서의 가능성을 보여주었다.

• KSBB Journal
• /
• 제30권6호
• /
• pp.268-274
• /
• 2015

3D printing technology has been used in various fields such as materials science, manufacturing, education, and medical field. A number of research are underway to improve the 3D printing technology. Recently, the use of 3D printing technology for fabricating an artificial tissue, organ and bone through the laminating of cell and biocompatible material has been introduced and this could make the conformity with the desired shape or pattern for producing human entire organs for transplantation. This special printing technique is known as "3D Bio-Printing", which has potential in biomedical application including patient-customized organ out-put. In this paper, we describe the current 3D bio-printing technology, and bio-materials used in it and present it's practical applications.

• Advances in materials Research
• /
• 제4권4호
• /
• pp.193-205
• /
• 2015

Contamination by bacterial strands is a major problem after bone replacement surgeries, so there is a great need to develop low cost biocompatible antibacterial bioactive scaffolds to be used in bone tissue engineering. For this purpose, nano-zinc doped hydroxyapatite with different zinc-concentrations (5, 10 and 15 mol%) was successfully prepared by the wet chemical precipitation method. The prepared powders were used to form porous scaffolds containing biodegradable Ca-cross-linked alginate (5%) in order to enhance the properties of alginate scaffolds. The scaffolds were prepared using the freeze-gelation method. The prepared powders were tested by X-ray diffraction; transmission electron microscope and Fourier transform infrared analyses, while the prepared scaffolds were investigated by Fourier transform infrared analyses, thermogravimetric analyses and measurement of the antibacterial properties. Best results were obtained from scaffold containing 15% mol zinc-doped hydroxyapatite powders and 5% alginate concentration with ratio of 70:30.

• 폴리머
• /
• 제34권3호
• /
• pp.185-190
• /
• 2010

전기방사공정에 의해 고분자의 나노 크기의 섬유를 만드는 기술로 널리 사용되어졌으며, 제작된 나노섬유는 그 높은 표면적과 형태학적 특성때문에 조직재생 공학분야에서 많이 사용되어져 왔다. 본 연구에서는 기존의 전기방사공정을 개선한 복합전기장을 이용하여 생분해성/생체적합성 poly(${\varepsilon}$-caprolactone) (PCL) 마이크로/나노섬유를 제작하였고, 기존의 나노섬유의 배열성보다 제어가 가능한 배열성을 갖는 공정시스템을 통하여 보다 우수한 배열성을 갖는 PCL 나노섬유를 제작하였다. 고배열된 PCL 나노섬유는 신경세포 재생을 위한 세포담체로서의 가능성을 확인하고자 신경세포(PC-12)를 배양하였으며 그 결과 높은 배열성을 갖은 PCL 나노섬유 매트에서 신경세포의 배열성이 얻어짐을 확인하였다.

• Macromolecular Research
• /
• 제14권5호
• /
• pp.530-538
• /
• 2006

There are three important components in tissue engineering: the cells, signaling factors (cytokines and growth factors), and scaffolds. To obtain finely engineered tissue, all three components should perform their individual functions and be fully integrated with each other. For the past few years, we have studied the characteristics of photodimerizable HA (CHA)/alginate (CA) composite materials. CHA/CA complex hydrogels, which were irradiated under UV light and, then treated with calcium ions, were found to have good biocompatibility, mechanical properties and water resistance for implantable tissue scaffolds. In this study, we introduced a cell growth factor (basic fibroblast growth factor; bFGF) into the CHA/CA scaffolds and studied its release behavior. We also introduced tetracycline hydrochloride and flurbiprofen into the same scaffolds as model activation factors and evaluated their release behaviors from the scaffolds. The drug release rate from the materials was influenced by various parameters, such as the degree of crosslinking, the cross linker type, the physico-chemical properties of the drug, and the amount of the drug in the polymer. The results indicated that the negatively charged CHA/CA composite materials showed sustained release behavior and that HA has a particularly strong negative charge, making it attractive toward tetracycline hydrochloride and bFGF, but repulsive toward flurbiprofen.

• 대한의용생체공학회:의공학회지
• /
• 제27권5호
• /
• pp.224-228
• /
• 2006

The object of this study is to investigate the potential of dual-electrospun polymer based structure for vascular tissue engineering, especially for the medium or small sue blood vessels. Polyurethane(PU), which is known to be biocompatible in this area, was electrospun with poly(ethylene oxide) (PEO). Concentration of PU was fixed at 20wt%, while that of PEO was set from 15 to 35wt%. Morphological features were observed by SEM image and measurement of porosity and cellular responses were tested before and after extracting PEO from the hybrid scaffolds by immersing the scaffolds into distilled water. The diameter of PEO fibers were ranged from 200nm to 500nm. The lower concentration of PEO tended to show beads. The porosity of the scaffolds after extracting PEO was highly increased with higher concentration of PEO as expected. Also, higher proliferation rate of smooth muscle cells was observed at higher concentration of PEO than at the lower concentration and without PEO. As conclusions, this dual electrospinning technique combined with PU and PEO is expected to overcome the current barrier of cell penetration by providing more space for cells to proliferation.