• 한국환경과학회지
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• 제21권9호
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• pp.1115-1129
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• 2012

The objective of this study was to analyze long-term annual and seasonal trends of water chemistry on landuse patterns and seasonal precipitation using 72 sampling sites within Namhan River watershed during 2001-2010. Water quality, based on multi-parameters of water temperature(WT), dissolved oxygen(DO), biochemical oxygen demand(BOD), chemical oxygen demand(COD), suspended solids(SS), total nitrogen(TN), total phosphorus(TP), and electric conductivity(EC) varied largely depending on monsoon rain and landuse patterns such as forest, cropland, and residence. Concentrations of BOD and COD as an indicator for organic matter pollution, increased during summer monsoon season at the cropland and residential streams. Values of TN and TP were higher in residential streams than in the forest and cropland streams. In the meantime, DO values had weak relations to the landuse patterns of forest and cropland cover. Water quality was worst in cropland and residential streams, and also most degradated in 4th order streams. Overall, our results suggest that efficient water quality management is required in the cropland and residential landuse streams.

• 한국미생물·생명공학회지
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• 제24권4호
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• pp.485-492
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• 1996

myo-Inositol, a growth factor for Saccharomyces cerevisiae (S. cerevisiae), has been known to be incorporated into phosphatidylinositol (PI), which is a kind of phospholipid in the cell membrane, by a membrane-associated PI-synthesizing enzyme. The deficiency of myo-inositol in S. cerevisiae adversely affected the membrane structure and function. On the basis of biochemical functions of myo-inositol, the effect of deficiency of myo-inositol on the aerobic glucose metabolism was investigated by measuring specific oxygen uptake rate (Q$_{O2}$) used as an indicator representing the respiratory capacity of S. cerevisiae in batch and continuous cultures. The respiratory capacity of aerobic glucose metabolism in S. cerevisiae was also monitored after glucose pulse-addition in a continuous culture (D=0.2, 1/hr), in which glucose was utilized through respiratory metabolism. The deficiency of myo-inositol was found to lead to both the decrease of the maximum specific oxygen uptake rate (Q$_{O2max}$) observed from the batch as well as in the continuous culture experiment and the decrease of the respiratory capacity of aerobic glucose metabolism of S. cerevisiae determined from the glucose pulse-addition experiment, in which the glucose flux into respiratory and fermen- tative metabolism was quantitatively analyzed.

• 한국토양비료학회지
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• 제46권1호
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• pp.32-39
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• 2013

The relationship between relative water contents of lettuce leaves and biochemical activities in lettuce was examined in this study to explore an adaptation response of lettuce to water stress from soils. Soil water contents and relative water contents of leaves were positively related to show $R^2$=0.8728. Hydrogen peroxide contents of leaves rapidly increased with reduction of soil water content, whereas soluble protein contents and dry matters rapidly decreased. And chlorophyll a and b contents of leaves decreased with increase in carotenoid content. Furthermore, the activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR) increased dramatically, and mRNA transcript levels of APX, MDHAR and DHAR also increased. Relationship of relative water content of lettuce leaves to hydrogen peroxide, to ascorbate peroxidase activity, to dehydroascorbate reductase activity, and to monodehydroascorbate reductase activity was shown to be positively correlated. It is highly plausible from this study that these enzyme activities could be developed as an indicator of water states in soils.

• 미생물학회지
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• 제22권4호
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• pp.265-269
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• 1984

E. coli $P^{RDI}$에서 DAP-영양요구성 변이주를 분리하였으며 이 변이주가 갖고 있는 plasmid의 안정성과 활성도를 확인하는 살험을 통하여 다음과 같은 결론을 얻었다. 1. Mutagen인 NG를 처리 후 DAP-변이주를 보다 쉽게 분리하기 위하여 항생제처리를 하였다. 이때 사용균주가 갖고 있는 plasmid내의 내성인자들 중에 carbr인자로 인하여 같은 계열에 있는 penicillin유도체들에 대해서는 교차내성을 갖고 있음이 확인되었다. 그러냐 penicillin과 같은 기능을 갖고 있으나 그 구조가 다른 cephalexin, cycloserine에 대해서는 교차 내성을 잘 나타내지 않았으므로 항생제처리로서는 cephalexin을 사용하였다. 2. 세균 접합을 통하여 DAP-균주의 특성을 동정하였다. 즉 nif-gene의 안정성과 활성도는 DAP-균주로 부터 plasmid를 전이반은 전이체에서 6-cyanopurine첨가로 확인하였다.

• Journal of Nutrition and Health
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• 제31권8호
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• pp.1283-1294
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• 1998

This study was conducted to investigate nutritional status by anthropometric meas-urement and to assess the validity of indicators for nutritional status of preschool children. Health examinations, including blood and urine tests, were done for 799 childern aged 2 to 6 years. The mean anthropometric measurements of height, weight , head circumference, and chest circumference were 90.2cm, 13.9kg, 47.7cm, 50.9cm for 2 years (n=13), 94.8cm, 15.2kg, 43.3cm, 47.0cm for 3 years(n=125), 100.7cm, 16.4kg, 46.2cm, 50.5cm for 4 years(n=272), 106.5cm, 18.1kg, 48.8cm, 53.8cm for 5 years(n=253), 113.0cm 20.4kg, 50.4cm 56.9cm for 6 years(n=136), respectively. All anthropometric measureemnts were significantly different by age and sex. The results of physical examinations excluding dental examination were normal for all studied children. Urine test results were also normal for the subjects. The most common blood type was A and the rarest one was type AB. The mean hemoglobin concentration was 11.84g/dl for boys(n=398), 11.86g/dl for girls, and 11.85kg/dl for all. The percentage of childeren whose hemoglobin concentration was less than 11.0g/dl was 21.6% for boys and 18.9% for girls. The percentage of children whose height for age, weight for age, and weight for height were in the rang of median $\pm$1 standard devication(S.D) was 71.7% , 65.6%, and 67.0% for each indicator. Obese childeren whose weight for height or weigth for age was more than median +2S.D. were 5.2% while tall children whose height for age was more than median +2S.D, were 1.8% . Past and recent nutritional status of most children(87.2%) were assessed as normal by the combination of three indicators(height for age, weight for age, weight for height). The percentate of coincidence between for height) and one assessed by anthropometric measurements (height for age, weight for age, weight for height) and one assessed by physicial was 59.6%, 69.3% and 67.8% for each indicator, respectively. The distribution of subjects by height for age was significantly different between normal and anemic groups. With these results , the nutritional status of children the studied was normal. The anthropometric measurements reflected the nutritional status of childeren relatively well. In conclusion, in comparison with biochemical meausurements anthropometric measurements are relatively simple and effective indicators for nutritional assessement of children in large health and nutrition suveys in Korea.

• Journal of Nutrition and Health
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• 제31권1호
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• pp.46-61
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• 1998

This study was carried out to evaluate the effects of vitamin D nutritional status on bone mineral density of adults (21-49 years). To attain the aim, we measured bone mineral density (BMD) of the subjects at distal radius by single-photon absorptiometry (SPA). Serum level of 25-hydroxyvitamin D(25-(OH)D) , known to be the best indicator of indicator of vitamin D status in humans was analyzed . The factors affecting this vitamin D level were also investigated in autumn in 122 young adults. Serum level of 25-(OH)D was measured by high pressure liquid chromatography(HPLC) and biochemical variables, general health status, time spent outdoors, and dietary intakes of the subjects. BMD of the male subjects was significantly greater than that of female subjects. Weight, activity and total energy expediture (TEE) showed a positive correlation with distal BMD. The mean level of serum 25-(OH)D was 24.4$\pm$11.0 ng./ml and by sex, 26.0$\pm$6.8ng/ml for males and 23.3$\pm$12.3ng/ml for females , the level was significantly higher in male (p<0.01). there was significant correlation between BMD at distal-radius and s-25(OH)D levels (p<0.001). The serum level fo parathyroid hormone (PTH) showed a negative correlation with BMD(p<0.05), with the more obvious correlation in females. Vitamin D intake was estimated to be 3.75$\pm$2.19ug/day in average. Among the nutrients studied, protein ,fat, calcium , and vitamin D intake were positively correlated with distal BMD. When food frequencies were concerned , milk and dairy products showed a significant positive correlation with the BMD level, and driedfoods, eggs , fats and oils, and cereals also showed a positive correlation. Time spent outdoors was estimated to be about 70 minutes in average and positively correlated with the distal BMD level(p<0.01). During the day, the specific time between 12 :00pm and 2:00pm showed the most significant correlation with BMD (p<0.001). Multiple regression analysis with the variables showed that distal BMD could be fit 31.9% by the time spent outdoors a day, intake of Ca and vitamin D, and TEE. The standardized estimates were 0.344 for vitamin D intake, 0.284 for Ca intake 0.179 for the time spent outdoors a day and 0.273 for TEE. For males, s-25*OH)D level, TEE and time spent outdoors during a day showed a significant correlation. For females, intake of Ca and vitamin D could fit about 27.1% of the distal BMD.

• Asian-Australasian Journal of Animal Sciences
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• 제26권6호
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• pp.788-794
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• 2013

Mastitis set apart as clinical and sub clinical is a disease complex of dairy cattle, with sub clinical being the most important economically. Of late, laboratories showed interest in developing biochemical markers to diagnose sub clinical mastitis (SCM) in herds. Many workers reported noteworthy alternation of acute phase proteins (APPs) and nitric oxide, (measured as nitrate+nitrite = NOx) in milk due to intra-mammary inflammation. But, the literature on validation of these parameters as indicators of SCM, particularly in riverine milch buffalo (Bubalus bubalis) milk is inadequate. Hence, the present study focused on comparing several APPs viz. ${\alpha}_1$-anti trypsin, ${\alpha}_1$-acid glycoprotein, fibrinogen and NOx as indicators of SCM in buffalo milk. These components in milk were estimated using standardized analytical protocols. Somatic cell count (SCC) was done microscopically. Microbial culture was done on 5% ovine blood agar. Of the 776 buffaloes (3,096 quarters) sampled, only 347 buffaloes comprising 496 quarters were found positive for SCM i.e. milk culture showed growth in blood agar with $SCC{\geq}2{\times}10^5$ cells/ml of milk. The cultural examination revealed Gram positive bacteria as the most prevalent etiological agent. It was observed that ${\alpha}_1$-anti trypsin and NOx had a highly significant (p<0.01) increase in SCM milk, whereas, the increase of ${\alpha}_1$-acid glycoprotein in infected milk was significant (p<0.05). Fibrinogen was below detection level in both healthy and SCM milk. The percent sensitivity, specificity and accuracy, predictive values and likelihood ratios were calculated taking bacterial culture examination and $SCC{\geq}2{\times}10^5$ cells/ml of milk as the benchmark. Udder profile correlation coefficient was also used. Allowing for statistical and epidemiological analysis, it was concluded that ${\alpha}_1$-anti trypsin indicates SCM irrespective of etiology, whereas ${\alpha}_1$-acid glycoprotein better diagnosed SCM caused by gram positive bacteria. NOx did not prove to be a good indicator of SCM. It is recommended measuring both ${\alpha}_1$-anti trypsin and ${\alpha}_1$-acid glycoprotein in milk to diagnose SCM in buffalo irrespective of etiology.

• 한국식품위생안전성학회지
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• 제32권5호
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• pp.396-403
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• 2017

본 연구는 국내 배추 주산단지 5지역을 대상으로 배추재배에 활용되는 농업용수를 각 지역의 수확 시기에 채취하여 위생지표세균(총대장균군, 대장균, 장구균)과 이화학 성분을 분석함으로써 농업용수의 수질의 오염도를 조사하기 위하여 수행되었다. 그 결과 지하수 보다 지표수에서 위생지표세균이 높은 수준으로 검출되었다. 지표수의 경우 총대장균군 1.96-4.96 log MPN/100 mL, 지하수의 경우 0-3.98 log MPN/100 mL 수준이었다. 장구균의 경우 지표수에서 95% (72/75), 지하수에서 22% (8/36) 빈도로 검출되었으며, 대장균의 경우 지표수에서 100% (72/75), 지하수에서 22% (8/36) 빈도로 검출되었다. 위생지표세균과 이화학성분의 상관관계를 조사한 결과, 지표수의 경우 대장균군과 대장균은 총인과 상관성을 보였으며, 장구균은 부유물질과 생물학적산소요구량에 상관성을 보였다. 지하수의 경우 위생지표세균은 부유물질과 화학적산소요구량에 상관성을 보였다. 본 연구의 결과는 엽채류 재배에 사용되는 농업용수의 미생물 기준을 설정하기 위한 기초자료로 활용될 수 있을 것으로 판단된다.

• Annals of Occupational and Environmental Medicine
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• 제35권
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• pp.4.1-4.11
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• 2023

Background: Shift work has been shown to increase the risk of cardiovascular disease (CVD) based on several evidences. The classic risk factors of CVD include age, hypertension, smoking, obesity and diabetes. Recently, the serum homocysteine level has been reported to be a valuable indicator of CVD risk. This study aimed to determine the variation in serum homocysteine level as a cardiovascular risk indicator among female workers according to shift work. Methods: The data of regular health examination of workers at an electronic manufacturing services company in Yeongnam region, South Korea in 2019 were examined in this study. The investigation was based on a cross-sectional study conducted on 697 female workers (199 day workers and 498 shift workers). The sociodemographic and biochemical characteristics were compared between day workers and shift workers. Through a logistic regression analysis, the odds ratio (OR) of the increased serum homocysteine level in relation to shift work was determined. Results: Compared to female day workers, female shift workers showed significantly higher level of serum homocysteine (8.85 ± 2.16 vs. 9.42 ± 2.04 μmol/mL; p = 0.001). The OR of day workers against shift workers was 1.81 (95% confidence interval [CI]: 1.25-2.63). With the adjustment of variables that may influence the level of serum homocysteine, the adjusted OR was 1.68 (95% CI: 1.09-2.60). Conclusions: The serum homocysteine level was significantly higher in shift workers than in day workers. It is thus likely to be a useful predictor of CVD in shift workers.

• Journal of Animal Science and Technology
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• 제62권5호
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• pp.595-604
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• 2020

This study investigated the effects of dietary rumen-protected L-tryptophan (TRP) supplementation (43.4 mg of L-tryptophan kg^-1 body weigt [BW]) for 65 days in Hanwoo steers on muscle development related to gene expressions and adipose tissue catabolism and fatty acid transportation in longissimus dorsi muscles. Eight Hanwoo steers (initial BW = 424.6 kg [SD 42.3]; 477 days old [SD 4.8]) were randomly allocated to two groups (n = 4) of control and treatment and were supplied with total mixed ration (TMR). The treatment group was fed with 15 g of rumen-protected TRP (0.1% of TMR as-fed basis equal to 43.4 mg of TRP kg^-1 BW) once a day at 0800 h as top-dressed to TMR. Blood samples were collected 3 times, at 0, 5, and 10 weeks of the experiment, for assessment of hematological and biochemical parameters. For gene study, the longissimus dorsi muscle samples (12 to 13 ribs, approximately 2 g) were collected from each individual by biopsy at end of the study (10 weeks). Growth performance parameters including final BW, average daily gain, and gain to feed ratio, were not different (p > 0.05) between the two groups. Hematological parameters including granulocyte, lymphocyte, monocyte, platelet, red blood cell, hematocrit, and white blood cell showed no difference (p > 0.05) between the two groups except for hemoglobin (p = 0.025), which was higher in the treatment than in the control group. Serum biochemical parameters including total protein, albumin, globulin, blood urea nitrogen, creatinine phosphokinase, glucose, nonesterified fatty acids, and triglyceride also showed no differences between the two groups (p > 0.05). Gene expression related to muscle development (Myogenic factor 6 [MYF6], myogenine [MyoG]), adipose tissue catabolism (lipoprotein lipase [LPL]), and fatty acid transformation indicator (fatty acid binding protein 4 [FABP4]) were increased in the treatment group compared to the control group (p < 0.05). Collectively, supplementation of TRP (65 days in this study) promotes muscle development and increases the ability of the animals to catabolize and transport fat in muscles due to an increase in expressions of MYF6, MyoG, FABP4, and LPL gene.