• Title/Summary/Keyword: bioaccumulation

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Metallothionein gene(pPMT)와 Manganese Transport Gene mntA(pZH3-5)를 포함한 재조합 Escherichia coli를 이용하여 수용액상에서의 Cadmium의 선택적 제거

  • Kim, Se-Gwon;Baek, Seung-Hak;Kim, Eun-Gi
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.426-429
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    • 2000
  • Recombinant E. coli JM109(pZH3-5/pMT) harboring manganese transport gene(mntA) and metal sequestering protein, metallothionein(MT), was cultivated to accumulate cadmium in aqueous phase. Bioaccumulation followed Michaelis-Menten type kinetics. Equilibrium isotherm showed Langmuir type isotherm. The optimum pH for $Cd^{2+}$ uptake was 7-7.5.

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Polychlorinated Biphenyls (PCBs) in the Bio-geochemistry of Oceans

  • Kannan, Narayanan
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.4
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    • pp.201-208
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    • 2007
  • Polychlorinated biphenyls (PCBs) are anthropogenic contaminants found globally in water, ice, soil, air and sediment. Modern analytical techniques allow us to determine these chemicals in environmental matrices at parts per trillion levels or lower. Environmental forensic on PCBs opens up new avenues of investigation such as transport and fate of water masses in oceans, sedimentation, onset of primary production, migration of marine mammals, their population distribution and pharmacokinetics of drugs inside organisms. By virtue of persistence, bioaccumulation, bioconcentration and structure-activity relationship PCBs emerge as unconventional chemical tracers of new sort.

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Evidences of Short-range and Long-range transport of Gas Phase PCBs in Rural Air (교외지역에서 대기 중 가스상 PCBs의 발생원 파악을 위한 연구)

  • 여현구;최민규;천만영;선우영
    • Proceedings of the Korea Air Pollution Research Association Conference
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    • 2002.11a
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    • pp.139-140
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    • 2002
  • 반 휘발성 유기화합물(Semivolatile organic compounds; 이하 SOCs)은 높은 중기압과 낮은 반응성 때문에 지구 전역에 폭넓게 존재하고 있다. SOCs중 Polychlorinatd Biphenyls(이하 PCBs)는 난분해성 및 친지질성에 특성으로 환경중에서 순환하기 때문에 최근 들어 맡은 관심의 대상이 되고 있다. 이들 대부분은 태초의 환경과 모든 matrix(예, 토양, 식물, 대기, 수계 등)에서 발견되어 지고 있다 이처럼 PCBs는 독성 유발물질로 관리되고 있으며 먹이연쇄를 통해 생농축성(bioaccumulation)을 야기 시킬 수 있다. (중략)

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Overlook of carbonaceous adsorbents and processing methods for elemental mercury removal

  • Bae, Kyong-Min;Kim, Byung-Joo;Park, Soo-Jin
    • Carbon letters
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    • v.15 no.4
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    • pp.238-246
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    • 2014
  • People have been concerned about mercury emissions for decades because of the extreme toxicity, persistence, and bioaccumulation of methyl Hg transformed from emitted Hg. This paper presents an overview of research related to mercury control technology and identifies areas requiring additional research and development. It critically reviews measured mercury emissions progress in the development of promising control technologies. This review provides useful information to scientists and engineers in this field.

Recent Development of Removal and Treatment of Toxic Heavy Metals by Microorganisms (유독 중금속 오염물질 처리를 위한 미생물균주의 최근 이용 및 개발)

  • 방상원;최영길;한명수
    • Korean Journal of Environmental Biology
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    • v.19 no.2
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    • pp.93-99
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    • 2001
  • There are several ways to remove and treat toxic heavy metals in the environment: chemical, physical and biological ways. The biological treatment utilizes the natural reactions of microorganisms living in the environments. These reactions include biosorption and bioaccumulation, oxidation and reduction, methylation and demethylation, metal - organic complexation and insoluble complex formation. The biological reactions provide a crucial key technology in the remediation of heavy metal-contaminated soils and waters. According to recent reports, various kinds of heavy metal species were removed by microorganisms and the new approaches and removal conditions to remediate the metals were also tried and reported elsewhere. This was mostly carried out by microorganisms such as fungi, bacteria and alga. In addition, a recent development of molecular biology shed light on the enhancing the microorganism's natural remediation capability as well as improving the current biological treatment.

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Estimated Bioaccumulation properties of Acetanilide using BCFWIN (BCF WIN을 이용한 Acetanilide의 생물농축특성 평가)

  • Kwon, Min-Jeong;Choi, Yoon-Ho;Song, Sang-Hwan;Park, Hye-Youn;Koo, Hyun-Ju;Park, Kwang-Sik
    • Environmental Analysis Health and Toxicology
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    • v.16 no.4
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    • pp.223-226
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    • 2001
  • Acetanilide is a High Production Volume Chemical, which is produced about 2,300 tons/year in Korea as of 1998 survey. Most is used as an intermediate for synthesis of pharmaceuticals and dyes. The chemical is one of seven chemicals, which are under the frame of OECD SIDS program sponsored by National Institute of Environmental Research of Korea. Regarding the information on the environmental fate. bioconcentration is one of important factor to estimate the environmental tranfer. However, measurement of bioconcentration needs high expense and time. For this reason, OECD recommends to use BCFWIN model to estimate bioconcentration of organic chemicals, BCFWIN estimates the bioconcentration factor (BCF) of an organic compound using the log octanol-water partition coefficient (Kow) of the compound. Structures are entered into BCFWIN through SMITES (Simplified Molecular Input Line Entry System) notations. The BCFWIN method classifies a compound as either ionic or non-ionic. ionic compounds include carboxylic acids, sulfonic acids and salts of sulfonic acids, and charged nitrogen compounds (nitrogen with a + 5 valence such as quaternary ammonium compounds). All other compounds are classified as non-ionic. In this study, bioaccumulation of acetanilide was estimated using BCFWIN model based on SMIIES notation, chemical name data and partition coefficient as one of environmental fate/distribution of the chemical elements.

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Bioaccumulation of Chromium Ions by Immobilized Cells of a Filamentous Cyanobacterium, Anabaena variabilis

  • Khattar, Jasvir I.S.;Sarma, Tangirala-A.;Singh, Davinder-P.;Sharma, Anuradha
    • Journal of Microbiology and Biotechnology
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    • v.12 no.1
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    • pp.137-141
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    • 2002
  • Anabaena variabilis ATCC 29413 grew in chromium (Cr) containing Chu-10 (basal) and nitrate-supplemented media, and the growth of the organism in $100{\mu}M$ chromium was found to be 50% of that in control medium. The growth in nitrate $({NO_3}^-)$ supplemented cultures was better as compared to cultures grown in basal medium. Free cells from basal and nitrate-supplemented media removed 5.2 and 7.4 nmol of chromium $mg^{-1}$protein in 8 h, respectively, from the medium containing $30{\mu}M$ chromium. The efficiency of chromium removal increased 7-fold in imidazole buffer (0.2 M, pH 7.0). A cell density equivalent to $100{\mu}g$ protein $ml^{-1}$ was found to be optimum for maximum Cr removal. Entrapment of cells in calcium-alginate beads did not affect the rate of Cr uptake by the cells. The efficiency of the laboratory-scale continuous flow bioreactor $(12.5{\times}2cm)$ loaded with alginate-immobilized cells (10 mg protein) and fed with $30{\mu}M$ chromium solution was compared at different flow rates. The efficiency of the bioreactor varied with flow rates. In terms of percent removal of Cr from influent, a flow rate of 0.1 ml $min^{-1}$ was found to be optimum for 6 h (54% Cr removal efficiency). Maximum amount of Cr (883 nmol) was removed by the cells in 3 h at a flow rate of 0.5 ml $min^{-1}$. The potential use of A. variabilis in removing Cr from industrial effluents is discussed.

Effect of Zinc Bioaccumulation on Survival Rate, Activity, Growth and Organ Structure of the Equilateral Venus, Gomphina veneriformis (Bivalvia: Veneridae) (아연의 체내축적이 대복의 생존, 운동성, 성장 및 기관계 구조에 미치는 영향)

  • Ju Sun-Mi;Lee Jae-Woo;Jin Young-Guk;Yu Jun;Lee Jung-Sick
    • Environmental Analysis Health and Toxicology
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    • v.21 no.2 s.53
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    • pp.115-126
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    • 2006
  • This study was conducted to find out survival rate, activity, growth and change of the organ structure of bivalves exposed to heavy metal. The results of the study confirmed that zinc (Zn) induces reduction of survival rate and activity, abnormality of organ structure of the equilateral venus, Gomphina veneriformis. Experimental groups were composed of one control condition and three Zn exposure conditions ($0.64mg\;Zn{\iota}^{1},\;1.07mg\;Zn{\iota}^{-1},\;1.79mg\;Zn{\iota}^{-1}$). As the concentration of zinc increased the accumulation of lipofucin increased in the digestive gland. Survival rate was the lowest in the lowest Zn exposure group at $0.64mg;Zn{\iota}^{-1}$. Growth was not significantly different between the control and exposure group. Activity. with the exception of the lowest Zn exposure group at $0.64mg\;Zn{\iota}^{-1}$, was similar between the control and exposure group. Histological analysis of organ system illustrated expansion of hemolymph sinus, loss of striated border of inner epidermis, increase in the number of mucous cell in the mantle. Also, histological degenerations as epithelial necrosis and hyperplasia of mucous cells are recognized in the gill and foot.

Heavy Metals in Sediments and Burrowing Bivalves (Sinonovacula constricta Lamarck) from Tidal Flats along the Saemankeum Area, Korea (새만금지역 갯벌 환경(패류, 저질)에서의 중금속 분포특성)

  • Hwang, Gab-Soo;Shin, Hyung-Seon;Kim, Kangjoo;Yeo, Sung-Koo;Park, Seongmin;Lim, Kyujae
    • Journal of Korean Society on Water Environment
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    • v.18 no.4
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    • pp.341-354
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    • 2002
  • Heavy metal concentration/distribution in sediments and bivalves from the tidal flats in Saemankeum coastal area, western Korea, were investigated, Among 6 sampling sites, S2, S5 and S6 showed the higher levels of Zn, Cr, Ni, Cu and Cd contamination and S1 did the higher level of Pb contamination than other sites, while S-4 showed the lowest levels of these metal contamination. Overally, the levels of Zn, Cu and Pb concentrations in Sinonovacula constricta were estimated to be relatively high. The shell lengths of the collected mussels were linearly related to their dry weights of the whole soft parts, but the mussels collected from S3 were in a poor nutrition, resulting in the distinctively high levels of metal concentrations in the body. It was shown that in S. constricta, Cr, Ni, Cu and Zn are distributed equally into the whole soft parts or a little more into the flesh, after absorption, while Fe, Cd, Pb and Mn are transferred more into the non-flesh parts than into the flesh parts. In S. constricta, the heavy metal concentrations in the flesh increase with those in the whole soft parts. The bioaccumulation factors(heavy metal concentration in S. constricta/heavy metal concentration in sediment) showed that, of the examined metals, Cd is the most cumulative in the body of S. constricta, followed by Zn and Cu, while Mn, Cr, Ni and Pb are not cumulative.