• Proceedings of the Korean Society of Sericultural Science Conference
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• 2005.04a
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• pp.51-51
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• 2005

• 한국환경농학회:학술대회논문집
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• 2009.07a
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• pp.349-349
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• 2009

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.10
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• pp.668-676
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• 2018

Most reservoirs in Korea have a low water depth and are small in size. Water pollution of the reservoir is serious because of the organic matter flowing from livestock wastewater, domestic sewage, and farmland. In this study, an attempt was made to improve the water purification effect by replacing the secondary dam installed in the depression area of the agricultural reservoir with the riprap dam. To evaluate the long-term performance, a riprap dam was installed in a reservoir in Gyeonggi province and water purification effect for 36 months was measured. The field test results showed that COD was 14.6%, SS 38.6%, T-N 9.5%, and T-P 11.2%. The concentrations in the influent water exhibited a significant change according to season, and the water purification effect increased with increasing concentration of influent water. The pollutant flowing into the lake from the depression area can be blocked effectively if the secondary riprap dam for water purification is installed in the agricultural reservoir.

• Magazine of the Korean Society of Agricultural Engineers
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• v.43 no.6
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• pp.174-186
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• 2001

Excessive outflow of pollutant loads resulting from rapid industrialization has unbalanced the water ecosystem, deteriorating the water quality environment severely. Therefore, measures for improving the water quality are necessary to maintain clean reservoir water and restore water-friendly spaces. A water quality model which is capable of simulating daily reservoir water quality was developed. The model had been applied to Masan reservoir and Wanggung reservoir in Korea. The model appeared to be satisfactory in representing the trend of water quality variations by comparing measured and simulated results. The model had been also applied to assess water purification techniques such as dredged pool, floating island and vegetation purification system. The model was considered to assess the effect of water purification techniques on reservoir water quality improvement. The results of water quality simulation for lake water purification techniques showed that a large facility would be needed to meet the targeted water quality of the reservoir when only one technique is applied. To effectively improve the quality of the polluted reservoir water, it is therefore recommended that pollutant sources should first be controlled, and a combination of the water purification techniques applied to make the utmost use of their secondary effects such as conservation of the reservoir volume capacity, establishment of a recreation space, promotion of bio-diversity, and improvement of the lake landscape.

• Journal of Bio-Environment Control
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• v.3 no.2
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• pp.136-144
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• 1994

A long- time aeration method was developed for purification of animal wastewater. Under repeated aereations of 4 hours on and 4 hours off, the higher removal rates were obtained which were in average of 99%, 96%, 92% and 50% for BOD, SS, total nitrogen and phosporous, respectively. In detail, the measured BOD concentrations of the influent and effluent were 2,700ppm and 40ppm while the SS concentrations in the primary chamber and of the effluent were about 3,000 and 110 ppm, respectively. Zeolite and activated carbon, applied for removing the nitrogen and phosphorous, showed a good absorption, especially zeolite for NH$_4$-N and activated carbon for NO$_3$-N and PO$_4$-P. The treatment cost per head by this method amounts to 1,923 won and it comes to 1.6% in the whole production cost. Therefore, this method is economically available with the half cost of the conventional activated sludge process.

• Korean Journal of Microbiology
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• v.38 no.4
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• pp.254-259
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• 2002

A psychrotrophic bacterium was isolated from Antarctic marine sediment and identified as Shewanella sp. species based on the biochemical properties and 16S rRNA sequence, and designated as Shewanella sp. L93. Extracellular protease produced by this strain was purified through ammonium sulfate precipitation, High-Q column chromatography, first gel permeation chromatography, BioScale Q2 ion exchange chromatography and second gel permeation chromatography, and basic properties of this enzyme were investigated.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.746-746
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• 2005

• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.852-858
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• 2008

An extracellular protease (Mc1) was isolated from the nematode-trapping fungus Monacrosporium cystosporium by gel filtration, anion-exchange, and hydrophobic interaction chromatographies. This protease had a molecular mass of approximately 38 kDa and displayed an optimal activity at pH 7-9 and $56^{\circ}C$ (over 30 min). Its proteolytic activity was highly sensitive to the serine protease inhibitor PMSF (phenylmethylsulfonylfluoride, 0.1 mM), indicating that it belonged to the serine-type peptidase group. The Michaelis constant ($K_m$) and $V_max$ for substrate N-Suc-Ala-Ala-Pro-Phe-pNA were $1.67{\times}10^{-4}\;M$ and 0.6071 $OD_{410}$ per 30 s, respectively. This protease could degrade a broad range of substrates including casein, gelatin, BSA (bovine serum albumin), and nematode cuticle. Moreover, the enzyme could immobilize the free-living nematode Panagrellus redivivus and the pine wood nematode Bursaphelenchus xylophilus, suggesting that it might playa role in infection against nematodes. The encoding gene of Mc1 was composed of one intron and two exons, coding for a polypeptide of 405 amino acid residues. The deduced amino acid sequence of Mcl showed 61.4-91.9% identity to serine proteases from other nematode-trapping fungi. Our results identified that Mcl possessed biochemical properties including optimal reaction condition and substrate preference that are different from previously identified serine proteases.

• Proceedings of the Microbiological Society of Korea Conference
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• 2005.05a
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• pp.223.4-223
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• 2005

• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1318-1323
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• 2004

This study describes the purification and characterization of a novel antihypertensive angiotensin 1­converting enzyme (ACE) inhibitory peptide from Saccharomyces cerevisiae. Maximal production of the ACE inhibitor from Saccharomyces cerevisiae was obtained from 24 h of cultivation at $30^{\circ}C$ and its ACE inhibitory activity was increased by about 1.5 times after treatment of the cell-free extract with pepsin. After the purification of ACE inhibitory peptides with ultrafiltration, Sephadex G-25 column chromatography, and reverse-phase HPLC, an active fraction with an $IC_{50}$ of 0.07 mg and $3.5\%$ yield was obtained. The purified peptide was a novel decapeptide, showing very low similarity to other ACE inhibitory peptide sequences, and its amino acid sequence was Tyr-Asp-Gly-Gly-Val-Phe-Arg-Val-Tyr-Thr. The purified inhibitor competitively inhibited ACE and also showed a clear antihypertensive effect in spontaneously hypertensive rats (SHR) at a dosage of 1 mg/kg body weight.