• Journal of the Korean association of regional geographers
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• v.14 no.1
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• pp.1-18
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• 2008

If there is no choice but to be urbanized, we should examine how human interference has had influences on the area to keep the river environment safe and stable. This study is aimed at finding improvement by examining the effect of human interference in Gapcheon river in Daejeon, which is developing fast. Distinctive features of human interference in Gapcheon river are followings. First, middle and upper reaches of Gapcheon river are being maintained and restored focusing on flood control but are scheduled to develop city and channel. Second, the rear area of middle and lower reaches of Gapcheon river is already developed into an urban district and solidified into artificial stream for leisure activities installing the artificial structure for fun. Third, lower reaches of Gapcheon river are in an unstable condition because of straightening waterway and developing rear area of river. Up to now, geographical features and bio-diversity of Gapcheon river has been ruined by the artificial management of river centered for technical engineering. From now on, followings should be considered for management of urban river. First, it is focused on maintaining eco system by itself than usage for human. Second, natural features of rear area of river should be taken into consideration and should be preserved before developing urban city and hills, back marsh, channel deposit and swamp to directly have an influence on river.

• Korean Journal of Poultry Science
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• v.36 no.4
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• pp.323-328
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• 2009

In order to determine the origin and genetic diversity among chicken breeds, mitochondrial (mt) DNA D-loop sequences have been widely used. In this study, 41 individuals from four breeds (Korean native chicken (Black and Brown) and two imported breeds, Rhode Island Red and Cornish) were used for identifying genetic relationships with other chicken breeds. We obtained ten haplotypes and the highest number of haplotype was represented by eight individuals each from haplotype 1 and haplotype 2. Neighbor-joining phylogenetic tree indicates that the black and brown Korean native chicken breeds were mixed in haplotype 2 and they were closely related with the red jungle fowl (Gallus gallus). We also investigated whether the D-loop hypervariable region in chicken mtDNA can be used for the breed identification marker. The results indicated that the combination of the SNPs in the D-loop region can be possibly used for the breed discriminating markers. The results obtained in this study can be used for designing proper breeding and conservation strategies for Korean native chicken, as well as development of breed identification markers.

• International Commerce and Information Review
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• v.13 no.3
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• pp.433-457
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• 2011

This study attempts to make a constructive contribution to the debate on which WTO rules accommodate trade measures for environmental purposes. Does trade undermine the regulatory efforts of governments. However, the theoretical dimensions are partly addressed on the several key questions. For instances, is economic integration through trade and investment a threat to the environment? to control pollution and resource degradation? Will economic grow driven by trade help us to move towards a sustainable use of the world's environmental resources? The growing world economy has been accompanied by environmental degradation including deforestation, losses in bio-diversity, global warming, air pollution, depletion of the ozone layer, overfishing and so on. The sheer number of us obviously put pressure on natural resources and ecological systems, and this pressure will counting to rise as we grow towards 10 billion in the next century. What is more, there is no indication that consumption per capita is slowing. The perceived costs of acting alone in terms of lost investments and jobs often take the stream out of regulatory initiatives. In the worst case scenario environmental community is fearful that international trade will magnify the effects of poor environmental polices in the world Generally, economic growth drive by trade may speed up the process of environmental degradation unless sufficient environmental safeguards are put in place. Under these circumstances, this paper attempts to make a constructive contribution to the study on which WTO rules accommodate trade measures for environmental purposes.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2005.09a
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• pp.1-23
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• 2005

Kochujang, a fermented hot pepper soybean paste, is a spicy condiment mixed with red pepper, boiled rice and mold fermented soybean (as koji) which is then fermented for several months. It has unique taste of hot, sweet, salty and savory flavor by all mixed ingredients by fermentation, and is one of most famous Korean traditional foods. The scale of the kochujang industry is about 200 billion won in Korea, and 7.8 million dollars and 4,500 tons in exports. Although it is still not a large part of the food industry in Korea, the scale of soy products will increase because it is a major subsidiary food in our dietary life and has grown from a homemade to a mass produced product. Kochujang has the largest market in the scale of sales among fermented soybean products and has merit because of its domestic base and its ability to grow to an international market due to its unique fermented taste. In order to make kochujang an international food, it Is necessary to study the scientific development of kochujang and the diversity of products using kochujang that are fitted to the taste of people around the world. In addition, participation in various exhibitions and food fairs supported by the government should help to promote kochujang throughout the world. In addition, these should be actively supported by the related businesses. Red pepper, a major ingredient in kochujang, has been widely studied and shown to have a positive effect on the reduction of fat, on the strengthening of the immune system, and on active biological functions. A fermented spice like kochujang has been shown to be beneficial for the body due to the process of fermentation rather than from the red pepper itself. If we prove the various biological functions of kochujang with a particular taste and flavor, it will energize a new market. It is necessary for business to make a continuous effort supported fly the scientific world and by government policy, in order to introduce this traditional food to the world. In the present day, the kochujang market is now on a firm basis and has been activated in the field of its study. Now, it is possible to achieve the birth of a secondary international market by concentrating on these efforts.

• Journal of Life Science
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• v.27 no.6
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• pp.694-701
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• 2017

Mycoplasma genitalium represents the smallest genome among mono-cultivable prokaryotes. To discover and compare the orthologs (conservative genes) among M. genitalium and 14 prokaryotes that are uncultivable and have less orthologs than M. genitalium, COG (clusters of orthologous groups of protein) analyses were applied. The analyzed prokaryotes were M. genitalium, one hyperthermophilic exosymbiotic archaeon Nanoarchaeum equitans, four intracellular plant pathogenic eubacteria of Candidatus Phytoplasma genus, and nine endosymbiotic eubacteria of phloem- and xylem-feeding insects. Among 367 orthologs of M. genitalium, 284 orthologs were conservative between M. genitalium and at least one other prokaryote. All 15 prokaryotes commonly have 29 orthologs, representing the significance of proteins in life. They belong to 25 translation-related, including 22 ribosomal proteins, 3 subunits of RNA polymerase, and 1 protein-folding-related. Among the 15 prokaryotes, 40 orthologs were only found in all four Candidatus Phytoplasma. The other nine Candidatus, all endosymbionts with insects, showed only a single common COG0539 (ribosomal protein S1), representing the diversity of orthologs among them. These results might provide clues to understand conservative genes in uncultivable prokaryotes, and may be helpful in industrial areas, such as handling prokaryotes producing amino acids and antibiotics, and as precursors of organic synthesis.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.135-135
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• 2017

The Gramene database (http://www.gramene.org) is a powerful online resource for agricultural researchers, plant breeders and educators that provides easy access to reference data, visualizations and analytical tools for conducting cross-species comparisons. Learn the benefits of using Gramene to enrich your lectures, accelerate your research goals, and respond to your organismal community needs. Gramene's genomes portal hosts browsers for 44 complete reference genomes, including crops and model organisms, each displaying functional annotations, gene-trees with orthologous and paralogous gene classification, and whole-genome alignments. SNP and structural diversity data, available for 11 species, are displayed in the context of gene annotation, protein domains and functional consequences on transcript structure (e.g., missense variant). Browsers from multiple species can be viewed simultaneously with links to community-driven organismal databases. Thus, while hosting the underlying data for comparative studies, the portal also provides unified access to diverse plant community resources, and the ability for communities to upload and display private data sets in multiple standard formats. Our BioMart data mining interface enable complex queries and bulk download of sequence, annotation, homology and variation data. Gramene's pathway portal, the Plant Reactome, hosts over 240 pathways curated in rice and inferred in 66 additional plant species by orthology projection. Users may compare pathways across species, query and visualize curated expression data from EMBL-EBI's Expression Atlas in the context of pathways, analyze genome-scale expression data, and conduct pathway enrichment analysis. Our integrated search database and modern user interface leverage these diverse annotations to facilitate finding genes through selecting auto-suggested filters with interactive views of the results.

• Horticultural Science & Technology
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• v.35 no.2
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• pp.232-242
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• 2017

The goal of marker-assisted backcrossing (MAB) is to significantly reduce the number of breeding generations required by using genome-based molecular markers to select for a particular trait; however, MAB systems have only been developed for a few vegetable crops to date. Among the types of molecular markers, SNPs (single-nucleotide polymorphisms) are primarily used in the analysis of genetic diversity due to their abundance throughout most genomes. To develop a MAB system in Chinese cabbage, a high-throughput (HT) marker system was used, based on a previously developed set of 468 SNP probes (BraMAB1, Brassica Marker Assisted Backcrossing SNP 1). We selected a broad-spectrum TuMV (Turnip mosaic virus) resistance (trs) Chinese cabbage line (SB22) as a donor plant, constructing a $BC_1F_1$ population by crossing it with the TuMV-susceptible 12mo-682-1 elite line. Foreground selection was performed using the previously developed trsSCAR marker. Background selection was performed using 119 SNP markers that showed clear polymorphism between donor and recipient plants. The background genome recovery rate (% recurrent parent genome recovery; RPG) was good, with three of 75 $BC_1F_1$ plants showing a high RPG rate of over 80%. The background genotyping result and the phenotypic similarity between the recurrent parent and $BC_1F_1$ showed a correlation. The plant with the highest RPG recovery rate was backcrossed to construct the $BC_2F_1$ population. Foreground selection and background selection were performed using 169 $BC_2F_1$ plants. This study shows that, using MAB, we can recover over 90% of the background genome in only two generations, highlighting the MAB system using HT markers as a highly efficient Brassica rapa backcross breeding system. This is the first report of the application of a SNP marker set to the background selection of Chinese cabbage using HT SNP genotyping technology.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.9
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• pp.1280-1286
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• 2016

This study was aimed to evaluate the in vitro effects of medicinal herb extracts (MHEs) on ruminal fermentation characteristics and the inhibition of protozoa to reduce methane production in the rumen. A fistulated Hanwoo was used as a donor of rumen fluid. The MHEs (T1, Veratrum patulum; T2, Iris ensata var. spontanea; T3, Arisaema ringens; T4, Carduus crispus; T5, Pueraria thunbergiana) were added to the in vitro fermentation bottles containing the rumen fluid and medium. Total volatile fatty acid (tVFA), total gas production, gas profiles, and the ruminal microbe communities were measured. The tVFA concentration was increased or decreased as compared to the control, and there was a significant (p<0.05) difference after 24 h incubation. pH and ruminal disappearance of dry matter did not show significant difference. As the in vitro ruminal fermentation progressed, total gas production in added MHEs was increased, while the methane production was decreased compared to the control. In particular, Arisaema ringens extract led to decrease methane production by more than 43%. In addition, the result of real-time polymerase chain reaction indicted that the protozoa population in all added MHEs decreased more than that of the control. In conclusion, the results of this study indicated that MHEs could have properties that decrease ruminal methanogenesis by inhibiting protozoa species and might be promising feed additives for ruminants.

• The Korean Journal of Pesticide Science
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• v.9 no.3
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• pp.205-213
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• 2005

This study was conducted to evaluate the response of the selected aquatic plants to sulfonylurea(SU)-herbicides, which have been used widely in rice-cultivating areas in Korea, and the possibility of using them as bio-indicator species for biodiversity conservation. The aquatic plants identified in Kangwon province where butachlor have been used extensively for many years were much more varied than those in Chonnam where SU-herbicides have been used extensively for many years. The growth responses of Monochoria korsakowii, Marsilea quadrifolia and Salvinia natans to sulfonylurea herbicides such as bensulfuron-methyl(BSM) and pyrazosulfuron-ethyl(PSE), were much more sensitive than those to butachlor and molinate. The $GR_{50}$ values of BSM and PSE for M. korsakowii, M. quadrifolia and S. natans were relatively very low when compared with those of butachlor and molinate. The number of internode and dry weight of M. quadrifolia which was exposed to herbicides runoff from rice fields were seriously affected. The acetolactate synthase(ALS) activities of M. quadrifolia sensitively inhibited by BSM and PSE were, and $I_{50}$(Inhibition 50%) were 5.6 and 2.1 nM, respectively.

• Ecology and Resilient Infrastructure
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• v.6 no.4
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• pp.179-190
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• 2019

In this study, a fish survey was conducted to investigate the habitat status of black shinner (Pseudopungtungia nigra) designated as endangered species level from the lower part of Yongdam Dam to the upper part of Daecheong Dam. Biodiversity analysis was performed on the basis of the number of fish emerged, and biological health and physical habitat environment were quantitatively analyzed using collected P. nigra. According to the survey, the habitat range of P. nigra was found from Banguri-myeon, Buri-myeon, Geumsan-gun, downstream of Yongdam Dam. The biodiversity analysis results of all species appeared to be low in overall dominance index and relatively high in species diversity and uniformity index. In addition, P. nigra collected in this study was confirmed to have normal growth and nutritional status by the full-length-weighted relativities and condition factor. The physical habitat environment of P. nigra was 0.3 - 0.6 m in depth, 0.3 - 0.7 m/s in flow rate, and bed materials showed high frequency of occurrence in the range of cobbles (64.0 - 256.0 mm) to boulders (>256.0 mm). These results are expected to be used as data for habitat restoration and management in the future as basic data on the spatial range and preferred physical habitat environment of P. nigra in Geumgang.