• Title/Summary/Keyword: bio materials

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Quantification of Inulo-oligosaccharides Using High pH Anion Exchange Chromatography with Pulsed Amperometric Detector (HPAEC-PAD)

  • Kang, Su-Il;Chang, Yung-Jin;Kim, Kyoung-Yun;Kim, Su-Il
    • Journal of Applied Biological Chemistry
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    • v.42 no.4
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    • pp.166-168
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    • 1999
  • Inulo-oligosaccharides (IOS, $F_n$, n=2-6) were purified from enzymatic hydrolysates of water-soluble extract of Jerusalem artichoke tubers. Quantification of inulo-oligosaccharides was done using high pH anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD) at the concentration range of 10-100 mg/L, which was compared with that of fructo-oligosaccharides (FOS, $GF_n$, n=1-7). Peak areas per mg IOS were higher than FOS at the same degree of polymerization (DP). Specific peak areas of IOS increased proportionally as DP increased up to six, in contrast to FOS showing no linearity.

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Adhesion Performance and Curing Behaviors of Acid-free Acrylic PSAs Using Two Types of Curing Agents (Acid-free 아크릴계 점착제의 접착 물성 및 경화거동 연구)

  • Lee, Seung-Woo;Park, Ji-Won;Kwon, Young-Eun;Kim, Hyun-Joong
    • Journal of Adhesion and Interface
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    • v.12 no.2
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    • pp.67-72
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    • 2011
  • Acrylic pressure-sensitive adhesives are used in many different parts in the world. But acrylic acid in PSAs may occur unexpected results such as corroding adherends or producing by-products when applied within electronic devices. This study employed acrylic PSAs based on 2-ethylhexyl acrylate (2-EHA), 2-hydroxyethyl acrylate (2-HEA) and butyl acrylate (BA) with different coating thickness. There are two types of curing agents. One is methylaziridine derivative (MAZ) and the other is aluminum acetylacetonate (AlACA). This study examined the adhesion performance and curing behaviors using peel strength, probe tack and gel fraction. Also, the viscoelastic properties of acrylic PSAs were investigated from Advanced rheometric expansion system (ARES).

Enhanced Production of Maltotetraose-producing Amylase by Recombinant Bacillus subtilis LKS88 in Fed-batch Cultivation

  • KIM, DAE-OK;KYUNGMOON PARK;JAE-WOOK SONG;JIN-HO SEO
    • Journal of Microbiology and Biotechnology
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    • v.7 no.6
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    • pp.417-422
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    • 1997
  • Recombinant Bacillus subtilis LKS88[pASA240] containing the amylase gene from Streptomyces albus KSM-35 was exploited in fed-batch cultivation for mass production of maltotetraose-producing amylase. The effects of dissolved oxygen, additional organic nutrients (peptone and yeast extract) and mixed carbon sources (glucose plus soluble starch) on amylase production were examined in fed-batch operations in an effort to determine the optimum conditions for a maximum amylase productivity. Under the optimum conditions, maximum amylase activity was about 4.2 times higher than that obtained in batch cultivations, indicating that mass production of maltotetraose-producing amylase could be accomplished in fed-batch cultivation of the recombinant B. subtilis strain.

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Research Trends and Considerations for u-Healthcare Security in Wellness Services (웰니스 서비스의 u-Healthcare 보안 연구동향 및 고찰)

  • Oh, Hyun Seok;Joo, Jae Woong;Kang, Won Min;Yi, Gangman;Jeong, Hwa Young;Park, Jong Hyuk
    • Proceedings of the Korea Information Processing Society Conference
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    • 2014.11a
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    • pp.457-459
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    • 2014
  • 최근 Wellness 산업과 스마트 디바이스가 융합되면서 기존의 질병관리 체계보다 앞서 예방하는 건강관리 u-Healthcare 가 개발되고 있다. 하지만 u-Healthcare 에서 취급되는 개인정보 및 의료정보는 메시지 탈취/변조로 인해 공격자에게 악용되어 의료사고를 유발하고 환자의 생명까지 앗아갈 수 있으며 또한 프라이버시 침해로 인해 사용자의 신변을 보장 받을 수 없다. 본 논문에서는 웰니스 서비스의 u-Healthcare 보안의 고려사항과 연구동향에 대해 살펴보고 현재 u-Healthcare 분야에서 보안이 적용된 시스템에 대해 분석하고 취급되는 정보보호의 중요성에 대해 고찰한다.

UV-curing Behaviors and Mechanical Properties of UV-cured Polylactic Acid (PLA)

  • Lee, Seung-Woo;Park, Ji-Won;Park, Cho-Hee;Kim, Hyun-Joong;Eom, Young-Geun
    • Journal of the Korean Wood Science and Technology
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    • v.41 no.2
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    • pp.134-140
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    • 2013
  • UV curing was introduced via a chemical treatment by adding small amounts of a hexafunctional acrylic monomer and a photoinitiator to improve the mechanical properties of PLA. This study also employed a semi-interpenetrated structured polymer network through the process of UV-curing. The UV curing behaviors were investigated using FTIR-ATR spectroscopy and gel fraction determination. Also, the tensile strength was investigated with different hexafunctional acrylic monomer contents and UV doses. The results showed that the crosslinking of UV-induced chemically treated PLA started at a low content of hexafunctional acrylic monomer, resulting in a significant improvement of the mechanical properties compared to those of neat PLA due to crosslinking.

Comparison of Main Compounds and Physiological Activities of Anthriscus sylvestris (L.) Hoffm. Roots and Aerial Parts Extracts (전호 지하부와 지상부 추출물의 주요성분 및 생리활성 비교)

  • Kim, Sol;Kim, Ha-Rim;Kim, Sang-Jun;Kim, Seon-Young
    • Korean Journal of Pharmacognosy
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    • v.52 no.2
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    • pp.77-83
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    • 2021
  • The number of people suffering from diabetes have been increased around the world. In this study, we investigated the antidiabetic and antioxidant effects of Anthriscus sylvestris(L.) Hoffm and its main compounds. It was divided into root(R) and aerial part(AP) for comparative analysis. Total polyphenol, total flavonoid content was higher in AP extract, but nodakenin content was higher in R(1169.13 ± 6.00 mg/g) extract. Antioxidant activity was also higher in AP extract. To compare antidiabetic efficacy, we analyzed the effects of R and AP extracts on ɑ-glucosidase inhibition(AGI), dipeptidyl peptidase-4(DPP-4) and protein tyrosine phosphatase(PTP)1B activity. R and AP extracts showed similar effects on AGI and DPP-4 activity in a concentration dependent manner, and there was no effect on PTP1B activation. Glucose uptake(139.51 ± 3.19%) in 3T3-L1 cells was more effective in the AP extract-treated group than the positive control, rosiglitazone, group. Both R and AP extracts were effective in protecting against pancreatic beta cell damage caused by hyperglycemia. These results suggest that Anthriscus sylvestris(L.) Hoffm. could be used as a candidate for diabetes treatment.

Label-free and sensitive detection of purine catabolites in complex solutions by surface-enhanced raman spectroscopy

  • Davaa-Ochir, Batmend;Ansah, Iris Baffour;Park, Sung Gyu;Kim, Dong-Ho
    • Journal of the Korean institute of surface engineering
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    • v.55 no.6
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    • pp.342-352
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    • 2022
  • Purine catabolite screening enables reliable diagnosis of certain diseases. In this regard, the development of a facile detection strategy with high sensitivity and selectivity is demanded for point-of-care applications. In this work, the simultaneous detection of uric acid (UA), xanthine (XA), and hypoxanthine (HX) was carried out as model purine catabolites by surface-enhanced Raman Spectroscopy (SERS). The detection assay was conducted by employing high-aspect ratio Au nanopillar substrates coupled with in-situ Au electrodeposition on the substrates. The additional modification of the Au nanopillar substrates via electrodeposition was found to be an effective method to encapsulate molecules in solution into nanogaps of growing Au films that increase metal-molecule contact and improve substrate's sensitivity and selectivity. In complex solutions, the approach facilitated ternary identification of UA, XA, and HX down to concentration limits of 4.33 𝜇M, 0.71 𝜇M, and 0.22 𝜇M, respectively, which are comparable to their existing levels in normal human physiology. These results demonstrate that the proposed platform is reliable for practical point-of-care analysis of biofluids where solution matrix effects greatly reduce selectivity and sensitivity for rapid on-site disease diagnosis.

Bio-Composite Materials Precursor to Chitosan in the Development of Electrochemical Sensors: A Critical Overview of Its use with Micro-Pollutants and Heavy Metals Detection

  • Sarikokba, Sarikokba;Tiwari, Diwakar;Prasad, Shailesh Kumar;Kim, Dong Jin;Choi, Suk Soon;Lee, Seung-Mok
    • Applied Chemistry for Engineering
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    • v.31 no.3
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    • pp.237-257
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    • 2020
  • The role of nano bio-composites precursor to chitosan are innumerable and are known for having different applications in various branches of physical sciences. The application to the sensor development is relatively new, where only few literature works are available to address the specific and critical analysis of nanocomposites in the subject area. The bio-composites are potential and having greater affinity towards the heavy metals and several micro-pollutants hence, perhaps are having wider implications in the low or even trace level detection of the pollutants. The nano-composites could show good selectivity and suitability for the detection of the pollutants as they are found in the complex matrix. However, the greater challenges are associated using the bio-composites, since the biomaterials are prone to be oxidized or reduced at an applied potential and found to be a hinderance for the detection of target pollutants. In addition, the materials could proceed with a series of electrochemical reactions, which could produce different by-products in analytical applications, resulting in several complex phenomena in electrochemical processes. Therefore, this review addresses critically various aspects of an evaluation of nano bio-composite materials in the electrochemical detection of heavy metals and micro-pollutants from aqueous solutions.

Soil Physical and Hydraulic Properties over Terrace Adjacent Four Major Rivers

  • Lee, Kyo Suk;Lee, Jae Bong;Lee, Myoung Yun;Joo, Ri Na;Lee, Dong Sung;Chung, Doug Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.3
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    • pp.235-241
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    • 2016
  • The soil does not only serve as a medium for plant growth but also for engineering construction purposes. It is very weak in tension, very strong in compression and fails only by shearing. The behaviour of the soil under any form of loading and the interactions of the earth materials during and after any engineering construction work has a major influence on the success, economy and the safety of the work. Soils and their management have therefore become a broad social concern. A limitless variety of soil materials are encountered in both agronomy and engineering problems, varying from hard, dense, large pieces of rock through gravel, sand, silt and clay to organic deposits of soft compressible peat. All these materials may occur over a range of physical properties, such as water contents, texture, bulk density and strength of soils. Therefore, to deal properly with soils and soil materials in any case requires knowledge and understanding of these physical properties. The desired value of bulk density varies with the degree of stability required in construction. Bulk density is also used as an indicator of problems of root penetration,soil aeration and also water infiltration. This property is also used in foundation engineering problems. While not conforming to standard test procedures, this work attempts to add to the basic information on such important soil parameters as water content, bulk density.

Evaluation of the grouting in the sandy ground using bio injection material

  • Kim, Daehyeon;Park, Kyungho
    • Geomechanics and Engineering
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    • v.12 no.5
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    • pp.739-752
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    • 2017
  • This study was intended to evaluate the improved strength of the ground by applying the bio grouting method to a loose sandy ground. The injection material was prepared in the form of cement-like powder, with the bio injection material produced by microbial reactions. The grouting test was conducted under the conditions similar to the field where the bio injection material can be applied. In addition, the injection materials (cement and sodium silicate No. 3) used for Labile Waterglass (LW) method and the conventional grouting methodwere prepared through a two-solution one-step process. The injection into the specimens was done at a pressure of 150 kPa and then, with a bender element, their moduliof elasticity were measured on the 7th, 14th, 21st and 28th curingdays to analyze their strengths according to the duration of curing. It was confirmed that in all injection materials the moduli of elasticity increased over time. In particular, when 30% of the bio injection material was added to 100% cement, the modulus of elasticity tended to increase by about 15%. This confirmed that the applicability became higher when the bio injection material was used in place of the conventional sodium silicate.