• Journal of the Korean Society of Food Science and Nutrition
• /
• v.18 no.4
• /
• pp.403-409
• /
• 1989

Dietary fiber(DF) content and their properties were analyzed in some common vegetables such as Squash(leaf), Water cress, Garland chrysanthemum, Leek and sweet potato(stem). DF was analyzed by the method of detergent fractionation. Values for neutral detergent fiber(NDF), acid detergent fiber(ADF), lignin, hemicellulose were obtained. NDF for most samples was $13.83%{\sim}24.31%$, ADF was $11.99%{\sim}21.03%$, lignin was around $0.56%{\sim}3.00%$, hemicellulose was about $1.33%{\sim}3.88%$, cellulose was $9.08%{\sim}14.87%$ except sweet potato(leaf) (20.47%). This paper describes two properties of the fiber-of commonly eaten vegetables ; water-holding capacity(WHC) and metal binding. The capacity of the acetone dried powder (ADP) to hold water was estimated. The WHC measurements differed from 7.49 per 9 of ADP for Water cress to 11.09 per 9 of ADP for Leek. The binding of Fe, Zn in NDF. ADF obtained from five fiber sources was examined under two pH conditions ; 1) pH2.1, 2) pH6.1. Fe, Zn binding increased with higher pH and differed from DF sources.

• The Journal of Natural Sciences
• /
• v.8 no.2
• /
• pp.35-41
• /
• 1996

To find the substrate interacting site of the MCAT1, charged amino acid residues in the transmembrane domain were changed to opposite charged amino acids and studied the arginine uptake, gp70 binding, efflux and protein expression using the Xenopus oocyte expression method. Among the five mutants of MCAT1, the D403K showed the most interesting characteristics, which had normal gp70 binding but low arginine uptake function, that means the normal expression on the membrane but decreased transport function. All mutants except K211E showed decreased arginine efflux, and kinetic study showed decreased Vmax. Together, Clu(403) residue of MCAT1 may show the possible substrate interacting site in the transmembrane domain of MCAT1.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.26 no.6
• /
• pp.1561-1569
• /
• 2016

As the use of the sensor device increases in IoT environment, the need for device security is becoming more and more important When a sensor device is deployed in IEEE 802.15.4-based LoWPAN, it has to perform the join operation with PAN Coordinator and the binding operation with another device. In the join and binding process, authentication and key distribution of the device are performed using the pre-distributed network key or certificate. However, the network key used in the conventional method has problems that it's role is limited to the group authentication and individual identification is not applied in certificate issuing. In this paper, we propose a secure join and binding protocol in LoWPAN environment that solves the problems of pre-distributed network key.

• Computers and Concrete
• /
• v.11 no.4
• /
• pp.305-316
• /
• 2013

This paper aims to study the effect of measurement methods and cracking on chloride transport of concrete materials. Three kinds of measurement methods were carried out, including immersion test, rapid migration test and steady-state migration test. All of these measurements of chloride transport show that chloride ion diffusion coefficient decreased with the reduction of water to cement ratio. Results of the immersion test were less than that of rapid migration test and steady-state migration test. For the specimen of lower water to cement ratio, the external electrical field has little effect on chloride binding relatively. Compared with the results obtained by these different measurement methods, the lower water to cement ratio may cause smaller differences among these different methods. The external voltage can reduce chloride binding of concrete, and the higher electrical field made a strong impact on the chloride binding. Considering the effect of high voltage on the specimen, results indicate that results based on the steady-state migration test should be more reasonable. For cracked concrete, cracking can accelerate the chloride ion diffusion.

• The KIPS Transactions:PartC
• /
• v.17C no.2
• /
• pp.153-158
• /
• 2010

Mobile IPv6 is one of method can keep Mobile node's session. To solve legacy Mobile IPv4's triangular routing problem, in Mobile IPv6, Mobile Node could directly communicate with Correspond node by Binding Update. But, attacker could interfere Return Routability Procedure that is Correspond node check Home address and Care of address reachable. At this result, Attacker is able to hijack Session to correspond node from Mobile node. In This paper, We propose new Binding Update scheme for solving that problem. Our approach is that MN gives association both home token and care of token using onewayness of keyed hash fuction. From security and performance analysis, we can see that proposed binding Update Scheme can achieve stronger security than legacy scheme and at the same time requires minimal computational overhead.

• Bulletin of the Korean Chemical Society
• /
• v.14 no.6
• /
• pp.726-732
• /
• 1993

A comparative study on cadmium(II) complexation with three well characterized humic acids (SHA: soil humic acid from the Okchun Metamorphic Belt; AqHA: aquatic humic acid from Gorleben underground aquifer, Germany; CoHA: commercially available humic acid from the Aldrich Co.) was carried out in 0.1 M $NaClO_4$ at different solution pH(5.0, 5.5, and 6.0) using the ultrafiltration technique. The maximum binding ability (MBA) of the humic acids for cadmium(II) was observed to vary with their origins and solution pH. The results suggest that 1 : 1 complex predominates within the experimental range, and the conditional stability constants were calculated based on the assumption of cooperative binding, yielding log K values that were quite similar (CoHA: 4.17${\pm}$0.08; AqHA: 4.14${\pm}$0.07; SHA: $4.06{\pm} 0.12\;l\;mol^{-1}$ at pH 6.0) irrespective of humic acid origins or pH. By contrast a nonlinear Schatchard plot was obtained, using the cadmium(II) ion selective electrode speciation analysis method, which indicated that humic acid may have two or more classes of binding sites, with $log\;K_1\;and\;log\;K_2$ of 4.73${\pm}$ 0.08 and $3.31{\pm}0.14\;l\;mol^{-1}$ respectively.

• Bulletin of the Korean Chemical Society
• /
• v.22 no.9
• /
• pp.953-957
• /
• 2001

The specificity of conjugation site for coating ligands was investigated using toluene-bovine serum albumin (BSA) conjugates in which BSA was conjugated at the position of o-, m-, and ${\rho}-toluic$ acid. Toluene-BSA conjugated at ${\rho}-position$ showed a binding activity of about 89-95%, whereas, those conjugated at o- and m-position of toluene exhibited a binding activity of 5 and 11%, respectively. On the basis of the above result, coating ligands with various proteins (OVA, BSA, KLH) were compared by conjugating with $\rho-toluic$ acid, and toluene-KLH was considered as the best coating ligand for this ELISA. Indirect competitive ELISA method was developed using anti-toluene antibody and $\rho-position$ conjugated toluene-KLH. The dose-response curve constructed after kinetic and optimization studies showed a 1${\times}$10-4 - 1${\times}$102 mM detectable response range with 0.1 ${\mu}M$ detectability. In specificity test of the antibody, the binding capabilities of aromatic compounds substituted with nitro-, alkyl-, chloro-, and hydroxyl group were larger rather than those of aromatic compounds (benzene, toluene and xylene) themselves. Also, tests with soil and water samples that had been spiked with toluene resulted in 102.7-113.7% recovery.

• Bulletin of the Korean Chemical Society
• /
• v.21 no.4
• /
• pp.393-400
• /
• 2000

2,2￠-Dihydroxyazobenzene (DHAB) was attached to poly(ethylenimine) (PEI) to obtain DHAB-PEI. Spectral titration revealed that uranyl, Fe(III), Cu(II), and Zn(II) ion form 1 : 1-type complexes with DHAB attached to PEI. Formation constants for the metal complexes formed by the DHAB moieties of DHAB-PEI were mea-sured by using various competing ligands. The results indicated thatthe concentrations of uranyl, Fe(III), and Cu(II) ions can be reduced to 10 -16 -10 -23 M at p 8 with DHAB-PEI when the concentration of the DHAB moiety is 1 residue M. By using cyanuric chloride as the coupling reagent, DHAB-PEI was immobilized on Sephadex G-25 resin to obtain DHAB-PEI-Seph. Binding of uranyl,Fe(III), Cu(II), and Zn(II) ion by DHAB-PEI-Seph was characterized by using competing ligands. A new method has been developed for characteriza-tion of metal sequestering ability of a chelating resin. Formation constants and metal-binding capacity of two sets of binding sites on the resin were estimated for each metal ion. DHAB-PI-Seph was applied to recovery of metals such as uranium,Fe, Cu, Zn, Pb, V, Mn, and W from seawater. The uranium recovery from seawaterby DHAB-PEI-Seph does not meet the criterion for economical feasibility partlydue to interference by Fe and Zn ions. The seawater used in the experiment was contaminated by Fe and Zn and, therefore, the efficiency of uranium extractionfrom seawater with DHAB-PEI-Seph could be improved if the experiment is carried out in a cleaner sea.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.17 no.3
• /
• pp.91-100
• /
• 2007

In this paper, We design and propose a protocol for supporting secure and efficient mobility in integrating fast handover and HMIPv6. In the proposed protocol which is AAA-based HMIPv6, if the MN enters the MAP domain for the first time, then it performs an Initial Local Binding Update for authentication. We propose a secure Fast Handover method using the ticket provided by MAP, which includes the secret key for authentication. Also, we analyze and compare security properties of our proposed scheme with those of other scheme using various attack scenario.

• Mass Spectrometry Letters
• /
• v.13 no.4
• /
• pp.115-124
• /
• 2022

Emerging evidence has shown that RNA-binding proteins (RBPs) dynamically regulate all aspects of RNA in cells and involve in major biological processes of RNA, including splicing, modification, transport, transcription and degradation. RBPs, as powerful and versatile regulatory molecule, are essential to maintain cellular homeostasis. Perturbation of RNA-protein interactions and aberration of RBPs function is associated with diverse diseases, such as cancer, autoimmune disease, and neurological disorders. Therefore, it is crucial to systematically investigate the RNA-binding proteome for understanding interactions of RNA with proteins. Thanks to the development of the mass spectrometry, a variety of proteome-wide methods have been explored to define comprehensively RNA-protein interactions in recent years and thereby contributed to speeding up the study of RNA biology. In this review, we systematically described these methods and summarized the advantages and disadvantages of each method.