• Journal of Marine Bioscience and Biotechnology
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• v.16 no.1
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• pp.45-54
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• 2024

Microalgae have great potential in the biomedical and pharmaceutical industries as a new type of bioreactor that can produce proteins for specific purposes, including recombinant proteins, pharmaceuticals, and industrial enzymes. Despite the production advantages and importance of microalgae-based expression systems, studies on secretion efficiency are limited. In this study, for stable expression and efficient secretion of the heterologous protein (human SCF and human INFγ) in Chlorella vulgaris, we constructed SP:hSCF:His and SP:hINFγ:His plant binary vectors using the signal peptide (SP) of Chlamydomonas reinhardtii, and we obtained stable transformants through the effective agrobacterium-mediated transformation of these vectors. Transformants with accurately inserted hSCF and hINFγ demonstrated stably increased mRNA and protein expression using RT-PCR and western blotting under the same culture conditions. Following the analysis of the proteins secreted into the culture medium using ELISA, it was confirmed that hINFγ was effectively produced in the transformed C. vulgaris culture medium. The overall findings indicate that the combination of heterologous protein and SP may be crucial for ensuring the expression and secretion of recombinant proteins in Chlorella culture systems.

• Journal of Institute of Control, Robotics and Systems
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• v.19 no.7
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• pp.646-652
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• 2013

EU's Galileo project is a market-based GNSS (Global Navigation Satellite System) that is under development. It is expected that Galileo will provide the positioning services based on new technologies in 2020s. Because Galileo E1 signal for OS (Open Service) shares the same center frequency with GPS L1 C/A signal, CBOC (Composite Binary Offset Carrier) modulation scheme is used in the E1 signal to guarantee interoperability between two systems. With E1 signal consisting of a data channel and a pilot channel at the same frequency band, there exist several options in designing signal acquisition for Assisted-Galileo receivers. Furthermore, compared to SNR worksheet of Assisted-GPS, some factors should be examined in Assisted-Galileo due to different correlation profile and code length of E1 signal. This paper presents SNR worksheets of Galileo E1 signals in E1-B and E1-C channel. Three implementation losses that are quite different from GPS are mainly analyzed in establishing SNR worksheets. In the worksheet, hybrid long integration of 1.5s is considered to acquire weak signal less than -150dBm. Simulation results show that the final SNR of E1-B signal with -150dBm is 19.4dB and that of E1-C signal is 25.2dB. Comparison of relative computation shows that E1-B channel is more profitable to acquire the strongest signal in weak signal environment. With information from the first satellite signal acquisition, fast acquisition of the weak signal around -155dBm can be performed with E1-C signal in the subsequent satellites.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.6
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• pp.201-208
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• 2014

In this paper, we propose the classifier structure design algorithm using DTW. Proposed algorithm uses DTW result to design the binary tree architecture based on the SVM which classify the multi-class data. Design the binary tree architecture for Support Vector Machine(SVM-BTA) using the threshold criterion calculated by the sum columns in square matrix which components are the reference data from each class. For comparison the performance of the proposed algorithm, compare the results of classifiers which binary tree structure are designed based on database and k-means algorithm. The data used for classification is 333 signals from 18 classes of underwater transient noise. The proposed classifier has been improved classification performance compared with classifier designed by database system, and probability of detection for non-biological transient signal has improved compare with classifiers using k-means algorithm. The proposed SVM-BTA classified 68.77% of biological sound(BO), 92.86% chain(CHAN) the mechanical sound, and 100% of the 6 kinds of the other classes.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.1749-1753
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• 2003

We proposed several systems for 1Giga bit Modem. The first, Binary ASK(Amplitude Shift Keying) system has a high speed shutter transmitter and no IF(Intermediate Frequency) receiver only by symbol synchronization. The advantage of proposed system is that circuitry is very simple without IF process. The disadvantage of proposed system are that line spectrum occurs interference to other channels, and enhancement to 4-level system is impossible due to its large SNR degradation. The second, Binary phase modulation system has a high speed shutter transmitter and IF-VCO(IF-Voltage Controlled Oscillator) control by base-band phase rotation. Polarity of shutter window is changed by the binary data. The window should be narrow same as above ASK. The advantage of proposed system is which error rate performance is superior. The disadvantage of proposed system are that Circuitry is more complex, narrow pull-in range of receiver caused by VCO and spectrum divergence by the non-linear amplifier. The third, 4-QAM(Quadrature Amplitude Modulation)system has a nyquist pulse transmitter and IF-VCO control by symbol clock. The advantage of proposed system are that signal frequency band is a half of 1GHz, reliable pull-in of VCO and possibility of double speed transmission(2Gbps) by keeping 1GHz frequency-band. The disadvantage of proposed system are that circuit complexity of pulse shaping and spectrum divergence by the non-linear amplifier.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2008.10a
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• pp.724-727
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• 2008

Studies on binary adder have been variously developed. According to those studies of critical worst delay and mean delay time of asynchronous binary adders, carry select adders (CSA) based on hybrid structure showed 17% better performance than ripple carry adders (RCA) in 32 bit asynchronous processors, and 23% better than in 64 bit microprocessor implemented. In the complicated signal processing systems such as RSA, it is essential to optimize the performance of binary adders which play fundamental roles. The researches which have been studied so far were subject mostly to addition algorithms or adder structures. In this study, we analyzed and designed adders in an asp;ect of synthesis method. We divided the ways of implementing adders into groups, each of which was synthesized with different synthesis options. Also, we analyzed the variously implemented adders to evaluate the performance and area so that we can propose a different approach of designing optimal binary adders.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.12 no.11
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• pp.1976-1979
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• 2008

Studies on binary adder have been variously developed. According to those studies of critical worst delay and mean delay time of asynchronous binary adders, carry select adders (CSA) based on hybrid structure showed 17% better performance than ripple carry adders (RCA) in 32 bit asynchronous processors, and 23% better than in 64 bit microprocessor implemented. In the complicated signal processing systems such as RSA, it is essential to optimize the performance of binary adders which play fundamental roles. The researches which have been studied so far were subject mostly to addition algorithms or adder structures. In this study, we analyzed and designed adders in an asp;ect of synthesis method. We divided the ways of implementing adders into groups, each of which was synthesized with different synthesis options. Also, we analyzed the variously implemented adders to evaluate the performance and area so that we can propose a different approach of designing optimal binary adders.

• Journal of Institute of Control, Robotics and Systems
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• v.22 no.11
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• pp.977-983
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• 2016

This paper proposes a novel code-tracking scheme to track the fine code synchronization for BOC (pn,n)-modulated global navigation satellite system signals in a repeat-back jamming environment. The correlation function of BOC (pn,n)-modulated signals has several peaks. The correlation function in the advanced offset region remains almost unchanged due to the repeat-back signals being received later than a line-of-sight signal in the same multipath signal receiving case. Additionally, the combined pseudo-random noise signal can be treated as repeat-back jamming signals, like multipath signals. In this paper, we propose a novel code-tracking scheme utilizing the advantages of using a combined pseudo-random noise signal in the advanced offset region and verify its performance through simulation.

• 제어로봇시스템학회:학술대회논문집
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• 1991.10b
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• pp.1801-1804
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• 1991

Described is a new type of control signal transmission system in which an optical fiber is used in place of metal wire cables. This optical transmission system is reliable against EMI and also eliminates the frequency band limitation on the metal wire cables. Since the Z80 CPU is used to distribute the instructions, many tasks can be carried out very easily, and many errors can be avoided. Although an experiment was carried out for 4 bit binary data, the number of bits can be increased to 6 or more without any degradation in reliability. Thus, a variety of applications can be expected to be actualized with this control signal transmission system.

• Journal of Electrical Engineering and Technology
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• v.12 no.4
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• pp.1449-1455
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• 2017

The vector control technique using low-resolution Hall-effect sensors has been widely used especially in consumer electronics. Due to electrical and/or mechanical unevenness related to binary-type Hall sensors, the calculated or estimated position information has discontinuities so called bumps, which causes the deterioration of vector control performance. In order to obtain a linearly changing position signal from low-precision Hall-effect sensors, this paper proposes a simple bumps in position signal removal algorithm that consists of a first-order observer with low-pass filtering scheme. The proposed algorithm has the feature of no needs for system parameters and additional estimation processes. The validity of the proposed method is verified through simulation and experimental results.

• 제어로봇시스템학회:학술대회논문집
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• 1992.10b
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• pp.285-289
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• 1992

This paper describes a new optimization technique for the design of traffic signal patterns. The proposed method uses a Genetic Algorithm for searching through the better signal patterns. Since the Genetic Algorithm is effective to search directly through a huge binary coded state spaces, the proposed design method has the following advantages over the conventional OR methods: (1) on-line optimization is available within a reasonable time, (2) there is no limitation to the types of signals to be optimized. Some computer simulations are carried out and its ability of getting high quality control in a short period is demonstrated.