• Applied Biological Chemistry
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• v.37 no.4
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• pp.248-254
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• 1994

Bromoxynil is an antidicot herbicide widely used on cereal crops and has a short half life in the soil. A bxn gene, encoding a specific nitrilase that converts bromoxynil to its primary metabolite 3,5-dibromo-4-hydroxybenzoic acid, was inserted in plant binary vector pGA482, and then introduced into tobacco and lettuce plants via Agrobacterium mediated leaf-disc transformation method. Transgenic plants with the bxn gene were selected by kanamycin and regenerated to whole plants. The regenerated transgenic plants were determined level of expression of bxn gene by Northern blot analysis. Leaf-disc analysis and pot-assay confirmed that the transgenic tobacco and lettuce plants were resistant to high doses of bromoxynil.

• Journal of Broadcast Engineering
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• v.10 no.1 s.26
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• pp.57-67
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• 2005

In the paper, we propose a TV-anytime codec that encodes and decodes TV-Anytime metadata according to the TV-Anytime specification so that the resulting binary TV-Anytime metadata can be transferred efficiently through the broadcasting network where the data bandwidth is restricted.. We describe the broadcasting environment that the TV-Anytime codec will be applied to, and the required functionalities of the software modules in detail. For the design of software modules, we show how to Implement the modules for metadata fragmentation. encoding, decoding, and the fragments management. The proposed TV-Anytime codec can be utilized as the core components to a personalized digital broadcasting system providing ECG(Electronic Content Guide) and segmentation information services according to TV-Anytime standard.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.4
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• pp.1499-1513
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• 2014

This paper proposes a novel blind image watermarking scheme exploiting Block Truncation Coding (BTC). Most of existing BTC-based watermarking or data hiding methods embed information in BTC compressed images by modifying the BTC encoding stage or BTC-compressed data, resulting in watermarked images with bad quality. Other than existing BTC-based watermarking schemes, our scheme does not really perform the BTC compression on images during the embedding process but uses the parity of BTC quantization data to guide the watermark embedding and extraction processes. In our scheme, we use a binary image as the original watermark. During the embedding process, the original cover image is first partitioned into non-overlapping $4{\times}4$ blocks. Then, BTC is performed on each block to obtain its BTC quantized high mean and low mean. According to the parity of high mean and the parity of low mean, two watermark bits are embedded in each block by modifying the pixel values in the block to make sure that the parity of high mean and the parity of low mean in the modified block are equal to the two watermark bits. During the extraction process, BTC is first performed on each block to obtain its high mean and low mean. By checking the parity of high mean and the parity of low mean, we can extract the two watermark bits in each block. The experimental results show that the proposed watermarking method is fragile to most image processing operations and various kinds of attacks while preserving the invisibility very well, thus the proposed scheme can be used for image authentication.

• The Transactions of the Korea Information Processing Society
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• v.5 no.3
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• pp.671-680
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• 1998

Network services, such as teleconferencing, remote diagnostics and education, and CSCW require multicasting. Multicast routing methods can be divided into two categories. One is the shortest path tree method and the other is the minimal Steiner tree method. The latter has an advantage over the former in that only one Steiner tree is needed for a group. However, finding a minimal Steiner tree is an NP-complete problem and it is necessary to find an efficient heuristic algorithm. In this paper, we present an evolutionary optimization method for finding minimal Steiner trees without sacrificing too much computational efforts. In particular, we describe a tree-based genetic encoding scheme which is in sharp constast with binary string representations usually adopted in convetional genetic algorithms. Experiments have been performed to show that the presented method can find optimal Steiner trees for given vetwork configurations. Comparitivie studies have shown that the evolutionary method finds on average a better solution than other conventional heustric algorithms.

• Journal of the Korea Convergence Society
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• v.10 no.2
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• pp.1-6
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• 2019

The proposed model is developed to minimize the loss of information in incomplete data including missing data. The first step is to transform the learning data to compensate for the loss information using the data extension technique. In this conversion process, the attribute values of the data are filled with binary or probability values in one-hot encoding. Next, this conversion data is input to the deep learning model, where the number of entries is not constant depending on the cardinality of each attribute. Then, the entry values of each attribute are assigned to the respective input nodes, and learning proceeds. This is different from existing learning models, and has an unusual structure in which arbitrary attribute values are distributedly input to multiple nodes in the input layer. In order to evaluate the learning performance of the proposed model, various experiments are performed on the missing data and it shows that it is superior in terms of performance. The proposed model will be useful as an algorithm to minimize the loss in the ubiquitous environment.

• Korean Journal of Medicinal Crop Science
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• v.15 no.5
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• pp.339-345
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• 2007

Field performance and morphological characterization was conducted on seven transgenic lines of Codonopsis lanceolata expressing ${\gamma}-TMT$ gene. The shoots were obtained from leaf explants after co-cultivation with Agrobacterium tume-faciens strain LBA 4404 harboring a binary vector pYBI 121 that carried genes encoding ${\gamma}-Tocopherol$ methyltransferase gene (${\gamma}-TMT$) and a neomycin phosphotransferase II gene (npt II) for kanamycin resistance. The transgenic plants were transferred to a green house for acclimation. Integration of T-DNA into the $T_0\;and\;T_1$ generation of transgenic Codonopsis lanceolata genome was confirmed by the polymerase chain reaction and southern blot analysis. The progenies of transgenic plants showed phenotypic differences within the different lines and with relative to control plants. When grown in field, the transgenic plants in general exhibited increased fertility, significant improvement in the shoot weight, root weight, shoot height and rachis length with relation to the control plants. However, all seven independently derived transgenic lines produced normal flower with respect to its shape, size, color and seeds number at its maturity. Indicating that the addition of a selectable marker gene in the plant genome does not effect on seed germination and agronomic performance of transgenic Codonopsis lanceolata. $T_1$ progenies of these plants were obtained and evaluated together with control plant in a field experiment. Overall, the agronomic performance of $T_1$ progenies of transgenic Codonopsis lanceolata showed superior to that of the seed derived non-transgenic plant. In this study, we report on the morphological variation and agronomic performance of transgenic Codonopsis lanceolata developed by Agrobacterium transformation.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.47 no.5
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• pp.25-33
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• 2010

Multi-media service has been changed into user based audio services, which service supports actively user's preference and interaction with the users. In the market, multi-media products which can support the highest audio-quality by using lossless audio technology have been released and object audio music which user can select the objects has been serviced. In this paper, we design user's preference information based object audio file format and audio scene description for storage and transmission media. The designed file format is designed based on MPEG-4 file format because high-quality audio codecs in MPEG-4 audio can be easily used and the track of file format can be flexibly controlled depend on the number of the instrument in music. The encoded audio data of each objects and encoded audio scene description by binary encoding that has independent track are packed in a file. The scene description for storage media is consist of full and object scene description, the scene description for transmission media has an essential description for object audio operation and a specific description for real audio sound. The designed file format based simulator is developed and it generates an object audio file with several scene descriptions. Also, the real audio sound is serviced by the interaction with user and the unpacked scene description.

• 한국균학회소식:학술대회논문집
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• 2014.10a
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• pp.9-9
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• 2014

Null mutants generated by targeted gene replacement are frequently used to reveal function of the genes in fungi. However, targeted gene deletions may be difficult to obtain or it may not be applicable, such as in the case of redundant or lethal genes. Constitutive expression system could be an alternative to avoid these difficulties and to provide new platform in fungal functional genomics research. Here we developed a novel platform for functional analysis genes in Magnaporthe oryzae by constitutive expression under a strong promoter. Employing a binary vector (pGOF1), carrying $EF1{\beta}$ promoter, we generated a total of 4,432 transformants by Agrobacterium tumefaciens-mediated transformation. We have analyzed a subset of 54 transformants that have the vector inserted in the promoter region of individual genes, at distances ranging from 44 to 1,479 bp. These transformants showed increased transcript levels of the genes that are found immediately adjacent to the vector, compared to those of wild type. Ten transformants showed higher levels of expression relative to the wild type not only in mycelial stage but also during infection-related development. Two transformants that T-DNA was inserted in the promotor regions of putative lethal genes, MoRPT4 and MoDBP5, showed decreased conidiation and pathogenicity, respectively. We also characterized two transformants that T-DNA was inserted in functionally redundant genes encoding alpha-glucosidase and alpha-mannosidase. These transformants also showed decreased mycelial growth and pathogenicity, implying successful application of this platform in functional analysis of the genes. Our data also demonstrated that comparative phenotypic analysis under over-expression and suppression of gene expression could prove a highly efficient system for functional analysis of the genes. Our over-expressed transformants library would be a valuable resource for functional characterization of the redundant or lethal genes in M. oryzae and this system may be applicable in other fungi.

• Journal of Life Science
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• v.23 no.11
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• pp.1317-1324
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• 2013

High-molecular weight glutenin subunits (HMW-GS) have been shown to play a crucial role in determining the processing properties of the wheat grain. We have produced marker-free transgenic rice plants containing a wheat Glu-1Bx7 gene encoding the HMG-GS from the Korean wheat cultivar 'Jokyeong' using the Agrobacterium-mediated co-transformation method. The Glu-1Bx7-own promoter was inserted into a binary vector for seed-specific expression of the Glu-1Bx7 gene. Two expression cassettes comprised of separate DNA fragments containing only Glu-1Bx7 and hygromycin phosphotransferase II (HPTII) resistance genes were introduced separately to the Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring Glu-1Bx7 or HPTII was infected to rice calli at a 3:1 ratio of Glu-1Bx7 and HPTII, respectively. Then, among 216 hygromycin-resistant $T_0$ plants, we obtained 24 transgenic lines with both Glu-1Bx7 and HPTII genes inserted into the rice genome. We reconfirmed integration of the Glu-1Bx7 gene into the rice genome by Southern blot analysis. Transcripts and proteins of the wheat Glu-1Bx7 were stably expressed in the rice $T_1$ seeds. Finally, the marker-free plants harboring only the Glu-1Bx7 gene were successfully screened at the $T_1$ generation.