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http://dx.doi.org/10.5352/JLS.2013.23.11.1317

Development of Marker-free Transgenic Rice for Increasing Bread-making Quality using Wheat High Molecular Weight Glutenin Subunits (HMW-GS) Gene  

Park, Soo-Kwon (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration)
Shin, DongJin (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration)
Hwang, Woon-Ha (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration)
Oh, Se-Yun (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration)
Cho, Jun-Hyun (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration)
Han, Sang-Ik (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration)
Nam, Min-Hee (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration)
Park, Dong-Soo (Department of Functional Crop, National Institute of Crop Science, Rural Development Administration)
Publication Information
Journal of Life Science / v.23, no.11, 2013 , pp. 1317-1324 More about this Journal
Abstract
High-molecular weight glutenin subunits (HMW-GS) have been shown to play a crucial role in determining the processing properties of the wheat grain. We have produced marker-free transgenic rice plants containing a wheat Glu-1Bx7 gene encoding the HMG-GS from the Korean wheat cultivar 'Jokyeong' using the Agrobacterium-mediated co-transformation method. The Glu-1Bx7-own promoter was inserted into a binary vector for seed-specific expression of the Glu-1Bx7 gene. Two expression cassettes comprised of separate DNA fragments containing only Glu-1Bx7 and hygromycin phosphotransferase II (HPTII) resistance genes were introduced separately to the Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring Glu-1Bx7 or HPTII was infected to rice calli at a 3:1 ratio of Glu-1Bx7 and HPTII, respectively. Then, among 216 hygromycin-resistant $T_0$ plants, we obtained 24 transgenic lines with both Glu-1Bx7 and HPTII genes inserted into the rice genome. We reconfirmed integration of the Glu-1Bx7 gene into the rice genome by Southern blot analysis. Transcripts and proteins of the wheat Glu-1Bx7 were stably expressed in the rice $T_1$ seeds. Finally, the marker-free plants harboring only the Glu-1Bx7 gene were successfully screened at the $T_1$ generation.
Keywords
Co-transformation; high-molecular-weight glutenin subunit (HMW-GS) protein; marker-free transgenic rice; wheat;
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