• 천문학회지
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• 제38권4호
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• pp.463-470
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• 2005

In this paper, we have made the component-based development of observational software for KASI solar imaging spectrograph (KSIS) that is able to obtain three-dimensional imaging spectrograms by using a scanning mirror in front of the spectrograph slit. Since 2002, the KASI solar spectrograph has been successfully operated to observe solar spectra for a given slit region as well as to inspect the response functions of narrow band filters. To improve its capability, we have developed the KSIS that can perform sequential observations of solar spectra by simultaneously controlling the scanning mirror and the CCD camera via Visual C++. Main task of this paper is to introduce the development of the component-based software for KSIS. Each component of the software is reusable on the level of executable file instead of source code because the software was developed by using CBD (component-based development) methodology. The main advantage of such a component-based software is that key components such as image processing component and display component can be applied to other similar observational software without any modifications. Using this software, we have successfully obtained solar imaging spectra of an active region (AR 10708) including a small sunspot. Finally, we present solar $H{\alpha}$ spectra ($6562.81{\AA}$) that were obtained at an active region and a quiet region in order to confirm the validity of the developed KSIS and its software.

• 한국터널지하공간학회 논문집
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• 제18권5호
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• pp.469-485
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• 2016

심부 터널 주변 암반의 파괴는 불연속면의 영향을 크게 받는 천부 터널 주변과 다르게 응력의 크기와 방향이 지배한다. 응력 지배 파괴의 양상은 응력 조건, 암석의 특성에 따라 연성과 취성으로 구분할 수 있으며 파석, 판상 파괴, 암석 파열 현상의 결과로 나타나는 V-형 홈 형태 취성 파괴 영역의 범위와 깊이는 심부 터널의 굴착과 보강 설계의 주요 인자이므로 이를 파악하는 것은 중요하다. 취성 파괴의 특성은 응력 조건에 따라 점착력 상실과 마찰력 전이로 구성된다는 점과 진행성 파괴라는 점이다. 본 연구는 이중 선형 절단 파괴 포락선과 탄성-탄소성 연계 해석과 점진적 탄소성 영역 확대라는 해석 절차와 방법을 도입하여 터널 주변 취성 암반의 파괴를 합리적으로 모사할 수 있는 3차원 수치 모델을 구현하였다. 이 수치 모델이 예상한 취성 파괴 영역의 깊이는 기존 사례 연구를 통한 경험식의 결과와 부합되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.131-131
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• 2017

Though the Platycodon grandiflorum, has a broad range of pharmacologic properties, but the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two-dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (${\geq}2-fold$) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose-1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, the frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). Taken together, the protein profile may provide insight clues for better understanding the characteristics of proteins and its metabolic activities in various explants of this essential medicinal plant P. grandiflorum.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.132-132
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• 2017

Plants, including Platycodon grandiflorum have been used globally across varied cultures as a safe natural source of medicines. From time immemorial, humans have relied on plants that could meet their basic necessities such as food, shelter, fuel and health. This study was executed to profile proteins from the hormone induced diploid and tetraploid roots using high throughput proteome approach. Two dimensional gels stained with CBB, a total of 64 differential expressed proteins were identified from the diploid root using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 20 differential expressed protein spots ( ${\geq}1.5-fold$) were analyzed using LTQ-FTICR MS whereas a total of 13 protein spots were up regulated and 7 protein spots were down-regulated. However, in the case of tetraploid root, a total of 78 differential expressed proteins were identified from tetraploid root of which a total of 28 differential expressed protein spots (${\geq}1.5-fold$) were analyzed by mass spectrometry whereas a total of 16 protein spots were up regulated and a total of 12 protein spots were down-regulated. However, proteins identified using iProClass databases revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding, oxidoreductase activity, transporter activity and isomers activity. The exclusive protein profile may provide insight clues for better understanding the characteristics of protein function and its metabolic activity that can help for the development of the nutritional and breeding aspects of this economically important medicinal plant.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.125-125
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• 2017

The roots of Platycodon grandiflorum are commonly used for treating bronchitis, asthma, tuberculosis, diabetes, and other inflammatory diseases. Since the molecular mechanism underlying the roots of the plant is unclear. Therefore, the present study was conducted to profile proteins from liquid cultured tetraploid roots of Platycodon grandi orum fl using high throughput proteome approach. Two-dimensional gels stained with CBB, a total of 659 differentially expressed proteins were identified from the liquid medium cultured tetraploid roots of which 32 proteins spots (${\geq}1.5-fold$) were sorted for mass spectrometry analysis. Out of these 32 proteins, a total of 15 proteins were up-regulated such as Serine carboxypeptidase-like 27, Transcription factor bHLH150, 60 kDa jasmonate-induced protein, Cytosolic Fe-S cluster assembly factor NBP35, Regulatory associated protein of TOR 2 and a total of 17 proteins were down-regulated such as Protein G1-like2, Phenylalanine ammonia-lyase, Fructokinase-2, Trihelix transcription factor GT-3a, Guanine nucleotide-binding protein alpha-1 subunit. However, the frequency distribution of identified proteins was carried out within functional categories based on molecular functions, cellular components, and biological processes. Functional categorization revealed that the most of the identified proteins from the explants were mainly associated with the nucleic acid binding, oxidoreductase, transferase activity, protein binding and hydrolase activity. In addition, the proteomic feedback of tetraploid roots of P. grandiflorum may potentially be used to understand the characteristics of proteins and their functions.

• 한국멀티미디어학회논문지
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• 제15권9호
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• pp.1093-1101
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• 2012

다시점 비디오는 3차원 정보를 표현하기 위한 영상으로 하나의 3차원 장면을 여러 시점에서 다수의 카메라로 촬영한 동영상이다. 영상들 사이에 존재하는 시간적 상관성과 화면간 상관성을 이용하는 다시점 비디오 부호화는 카메라의 수에 비례하여 데이터의 양이 늘어나기 때문에 계산량을 줄일 수 있는 다시점 비디오 부호화 기술이 필요하다. 본 논문에서는 다시점 비디오의 부호화 성능을 향상시키기 위한 효율적인 예측구조를 제안한다. 제안한 예측 구조는 다시점 비디오의 부호화 효율을 높이기 위하여 부호화되는 현재 화면과 현재 화면이 참조하는 참조 화면들과의 평균 거리, B계층 최대 인덱스 그리고 각 Bi 계층의 화면 수를 고려하였다. 제안한 예측 구조의 성능을 참조 예측 구조의 성능과 비교하였을 때 영상 화질 면에 있어서 제안한 예측 구조가 Fraunhofer-HHI의 계층적 B화면 구조보다 약 0.07~0.13 (dB) 성능 향상을 보였다. 발생되는 평균 초당 비트량에 있어서 제안한 예측 구조가 Fraunhofer-HHI의 계층적 B화면 구조보다 최대 6.5(Kbps) 감소하였다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.361.2-361.2
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• 2014

Transition metal dichalcogenides (MoS2, WS2, WSe2, MoSe2, NbS2, NbSe2, etc.) are layered materials that can exhibit semiconducting, metallic and even superconducting behavior. In the bulk form, the semiconducting phases (MoS2, WS2, WSe2, MoSe2) have an indirect band gap. Recently, these layered systems have attracted a great deal of attention mainly due to their complementary electronic properties when compared to other two-dimensional materials, such as graphene (a semimetal) and boron nitride (an insulator). However, these bulk properties could be significantly modified when the system becomes mono-layered; the indirect band gap becomes direct. Such changes in the band structure when reducing the thickness of a WS2 film have important implications for the development of novel applications, such as valleytronics. In this work, we report for the controlled synthesis of large-area (~cm2) single-, bi-, and few-layer WS2 using a two-step process. WOx thin films were deposited onto a Si/SiO2 substrate, and these films were then sulfurized under vacuum in a second step occurring at high temperatures ($750^{\circ}C$). Furthermore, we have developed an efficient route to transfer these WS2 films onto different substrates, using concentrated HF. WS2 films of different thicknesses have been analyzed by optical microscopy, Raman spectroscopy, and high-resolution transmission electron microscopy.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.356.1-356.1
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• 2014

The Isolation of few-layered transition metal dichalcogenides has mainly been performed by mechanical and chemical exfoliation with very low yields. in particular, the two-dimensional layer of molybdenum disulfide (MoS2) has recently attracted much interest due to its direct-gap property and potential application in optoelectronics and energy harvesting. However, the synthetic approach to obtain high-quality and large-area MoS2 atomic thin layers is still rare. In this account, a controlled thermal reduction-sulfurization method is used to synthesize large-MoOx thin films are first deposited on Si/SiO2 substrates, which are then sulfurized (under vacuum) at high temperatures. Samples with different thicknesses have been analyzed by Raman spectroscopy and TEM, and their photoluminescence properties have been evaluated. We demonstrated the presence of mono-, bi-, and few-layered MoS2 on as-grown samples. It is well known that the electronic structure of these materials is very sensitive to the number of layer, ranging from indirect band gap semiconductor in the bulk phase to direct band gap semiconductor in monolayers. This synthetic approach is simple, scalable, and applicable to other transition metal dichalcogenides. Meanwhile, the obtained MoS2 films are transferable to arbitrary substrates, providing great opportunities to make layered composites by stacking various atomically thin layers.

• 한국안광학회지
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• 제10권1호
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• pp.9-15
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• 2005

CR 렌즈에 가한 미세응력 크기의 시각화를 위한 편광기는 광원-편광판-시료-편광판-CCD-컴퓨터-chrome 변환 로 구성하였고, 각 편광판의 단계별 광 파의 $E_1$, $E_2$의 성분을 분석하여, 주 응력차 ${\sigma}_1-{\sigma}_2$와 무늬 차수(n)를 유도하였다. 시료의 광 축이 주 응력 방향과 일치하는 것으로부터 2 차원 모델을 결정할 수 있다. 광파에 작용하는 이중 굴절성과 위상 지연은 주 응력차(${\sigma}_1-{\sigma}_2$)에 비례하며, 최종 광 파의 세기는 $sin^2({\Delta}/2)$에 비례하고, ${\Delta}/2=n{\pi}$ (n=0, 1, 2, ${\ldots}$)일 때 흡광이 일어난다. 미세 응력에 의한 광탄성은 chrome 변환을 이용하여 분석 가능하였으며, 이미지는 뚜렷하였다.

• 한국작물학회지
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• 제60권1호
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• pp.97-106
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• 2015

Platycodon grandiflorum, commonly known as Doraji in Korea, has a wide range of pharmacologic properties, such as reducing adiposity and hyperlipidemia, and antiatherosclerotic effects. However, the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (${\geq}$ 2-fold) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose-1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). In that way, the exclusive protein profile may provide insight clues for better understanding the characteristics of proteins and metabolic activity in various explants of the economically important medicinal plant Platycodon grandiflorum.