• Title/Summary/Keyword: beta-HAD

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Comparison of the chemical compositions and nutritive values of various pumpkin ($Cucurbitaceae$) species and parts

  • Kim, Mi-Young;Kim, Eun-Jin;Kim, Young-Nam;Choi, Chang-Sun;Lee, Bog-Hieu
    • Nutrition Research and Practice
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    • v.6 no.1
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    • pp.21-27
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    • 2012
  • Pumpkins have considerable variation in nutrient contents depending on the cultivation environment, species, or part. In this study, the general chemical compositions and some bioactive components, such as tocopherols, carotenoids, and ${\beta}$-sitosterol, were analyzed in three major species of pumpkin ($Cucurbitaceae$ $pepo$, $C.$ $moschata$, and $C.$ $maxima$) grown in Korea and also in three parts (peel, flesh, and seed) of each pumpkin species. $C.$ $maxima$ had significantly more carbohydrate, protein, fat, and fiber than $C.$ pepo or $C.$ $moschata$ (P<0.05). The moisture content as well as the amino acid and arginine contents in all parts of the pumpkin was highest in $C.$ $pepo$. The major fatty acids in the seeds were palmitic, stearic, oleic, and linoleic acids. $C.$ $pepo$ and $C.$ $moschata$ seeds had significantly more ${\gamma}$-tocopherol than $C.$ $maxima$, whose seeds had the highest ${\beta}$-carotene content. $C.$ $pepo$ seeds had significantly more ${\beta}$-sitosterol than the others. Nutrient compositions differed considerably among the pumpkin species and parts. These results will be useful in updating the nutrient compositions of pumpkin in the Korean food composition database. Additional analyses of various pumpkins grown in different years and in different areas of Korea are needed.

A Study on the Effects of Perceived Service Quality in Korean Food Restaurants upon Customer Satisfaction and Behavioral Intention : Focused on the Mediating Roles of Trust (한식당의 인지된 서비스 품질이 고객 만족도 및 행동 의도에 미치는 영향 연구 : 신뢰의 매개 효과를 중심으로)

  • Jung, Hyo-Sun;Song, Min-Kyung;Lee, Sun-Lyung;Kang, Ok-Goo;Yoon, Hye-Hyun
    • Korean journal of food and cookery science
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    • v.25 no.5
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    • pp.545-556
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    • 2009
  • The purposes of this study were to understand the influence of perceived service quality in Korean food restaurants on customer satisfaction and behavioral intention and to empirically analyze whether trust plays a mediating role between both the relations of the perceived service quality and customer satisfaction and the perceived service quality and behavioral intention. Based on total 303 samples obtained from empirical research, this study reviewed the reliability and fitness of the research model and verified a total of 5 hypotheses using the Amos program. The hypothesized relationships in the model were tested simultaneously by using a structural equation model(SEM). The proposed model provided an adequate fit to the data, $X^2{_{(df131)}}$ = 536.566, p<.01, RMR .126, GFI .841, NFI .896, CFI .919, RMSEA .101. The SEM results showed that the perceived service quality(${\beta}$= .823, t = 9.392, p<.001) had a significant positive effect on trust had a significant positive effect on trust, and trust had a significant positive effect on customer satisfaction(${\beta}$ = .730, t = 14.202, p<.001) and behavioral intention(${\beta}$ = .239, t = 3.362, p<.001). Also, customer satisfaction had a significant positive effect on behavioral intention(${\beta}$ = .509, t = 6.978, p<.001). In addition, the effect of the perceived service quality in Korean food restaurants upon behavioral intention was found to be partially mediated by the trust.

The Effect of Job Characteristics and Job Satisfaction on Organizational Commitment of Hospital Foodservice Employees in Busan Area (부산지역 병원급식 조리종사자의 직무특성 및 직무만족이 조직몰입에 미치는 영향)

  • Kim, Min-Young;Lyu, Eun-Soon
    • Korean journal of food and cookery science
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    • v.32 no.6
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    • pp.745-753
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    • 2016
  • Purpose: This study was conducted to provide basic data for human resources management of hospital foodservice employees by determining their job characteristics, job satisfaction, and organizational commitment according to recent hospital environment changes. Methods: Our survey was administered to 248 hospital foodservice employees in Busan area from September 1 to September 25, 2014. A total of 158 questionnaires were used for final analysis. Results: The mean score of job characteristics showed significantly difference by work experience (p<0.001), annual salary (p<0.01), and cooking certification (p<0.05). Job satisfaction had significantly difference by the age (p<0.05), annual salary (p<0.001), and turnover intention (p<0.001). Organizational commitment showed significantly (p<0.001) difference by age, work experience, and annual salary. There was positive correlation (p<0.001) between organizational commitment and job characteristics, job satisfaction. Organizational commitment had significantly positive correlation with skill variety (p<0.001), feedback (p<0.01), and task significance (p<0.001) of job characteristics, with work (p<0.001), pay (p<0.001), and co-workers (p<0.001) of job satisfaction. Job characteristics (${\beta}=0.249$, p<0.001) and job satisfaction (${\beta}=0.380$, p<0.001) had positive influences on the organizational commitment(p<0.001). In sub factors of job satisfaction, work (${\beta}=0.291$, p<0.001) and pay (${\beta}=0.252$, p<0.01) had positive influences on organizational commitment. Conclusion: To develop the organizational commitment, hospital managers need to reinforce responsibility and fulfillment by job enrichment and to consider increasing salaries to get a higher satisfaction from foodservice employees.

STRUCTURAL ANALYSIS OF THE SOLUBLE FORM OF BOVINE DOPAMINE BETA-HYDROXYLASE

  • Hwang, On-You;Joh, Tong-Hyub
    • Toxicological Research
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    • v.6 no.1
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    • pp.99-107
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    • 1990
  • Dopamine beta-hydroxylase (DBH) is a neurotransmitter biosynthetic enzyme which catalyzes the conversion of dopamine to norepinephrine. Although structural studies of the mature form of this enzyme have been extensive, the culmination of these finding had been hightly controversial and contradictory. In this study, biochemical approaches were taken to characterize the structure of mature DBH. Soluble bovine DBH was purified from aderenal medulla. Three bands of 69 kDa, 72 kDa and 75 kDa which were physically separable and similar in structure were observed by SDS-PAGE. Furthermore, gas phase sequence analysis revealed that the 72 kDa band consists of two polypeptides which are present at equimolar concentrations and differed in that one had three extra amino acids at the N-terminus. Taken together, the soluble form of DBH exist in at least four forms, three identified by SDS-PAGE, one of which consists of two polypeptides as identified by N-terminal sequence analysis. The significance of these forms and their possible biosynthetic mechanisms are discussed.

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Studies on the Bis (amino-alkyl-oxo) Oxalate Derivatives As a Antidote of Lead poisoning (Bis (\beta$-amino-alkyl-oxo) oxalate 유도체의 납 중독 해독에 관한 연구)

  • 최선아;유미례;노영수;정성현;정세영
    • Environmental Analysis Health and Toxicology
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    • v.4 no.3_4
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    • pp.1-6
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    • 1989
  • The effects of his ($\beta$-amino-ethyl-oxo) oxalate and bis ($\alpha$-amino-methyl-oxo) oxalate on the toxicity of lead acetate in rast were examined. Rats were given intraperitoneally at the dose of lead acetate 45 mg/kg. The exposure of lead acetate showed the 70% decrease of ALAD ($\delta$-amino levulinic acid dehydratase) activity in red blood cell. In vivo 122 mg/kg administration of bis ($\beta$-amino-ethyl-oxo) oxalate and bis ($\alpha$-amino-methyl-oxo) oxalate showed the 50% increase of ALAD activity, whereas 149 mg/kg administeration of Ca-EDTA had no effect. In vitro, the same results were obtained. Both compounds had hemolytic activity at concentrations higher than that required for showing the 50% ALAD activity increase in vivo.

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Effect of Heating Temperature on Viable Yoghurt Culture and $eta$-Galactosidase in Yoghurt (Yoghurt의 가열처리가 젖산균 생균수 및 $eta$-galactosidase의 활성에 미치는 영향)

  • 이광희
    • The Korean Journal of Food And Nutrition
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    • v.6 no.3
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    • pp.208-210
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    • 1993
  • In order to know the influences of heat treatment of yoghurt on pH, $\beta$-galactosidase and viable cells, yoghurt sample was made by general method with Lactobacillus bulgaricus and Streptococcus thermophilus, and the changes in pH, $\beta$-galactosidase-activity and viable cell-count were determined during heating at 55$^{\circ}C$ and 7$0^{\circ}C$. The pH of yoghurt was not changed when the yoghurt was heated at 7$0^{\circ}C$, but at 55$^{\circ}C$ it decreased slightly. The stability of $\beta$-galactosidase was not affected markedly by heat treatment at 55$^{\circ}C$, but was rapidly inactivated at 7$0^{\circ}C$. The heat treatment of yoghurt at 55$^{\circ}C$ had the halb of viable cell in 1 hour, but the heat treatment at 7$0^{\circ}C$ had considerable effect on viable cell in 5 minutes.

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Synthesis of $\beta$-Alumina By Oxalate Coprecipitation Method and Its Crystallization Behavior (Oxalate 공침법에 의한 $\beta$-Alumina 합성과 결정화 거동)

  • 박용민;양유철;김형욱;박성수;손영국
    • Journal of the Korean Ceramic Society
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    • v.32 no.4
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    • pp.455-461
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    • 1995
  • To investigate the synthesis of $\beta$-Al2O3 and its crystallization behavior by oxalate coprecipitation method, the optimum pH range for oxalate coprecipitates has been theoretically calculated from the solubility products and the equilibrium constans of each metal ionic species and their solubility diagram wa obtained. The optimum pH range for oxalate coprecipitates at room temperature was estimated as <4. In experiment, we found that the optimum condition for oxalate coprecipitates was pH<1, which was not doped with pH controller. The Na+ ions were easily exchanged for the NH4+ ions of NH4OH which was used as pH controller, and those NH4+ ions were supposed to affect the crystallization behavior of $\beta$-Al2O3. The thermal decomposition of all complexes was almost complete below 40$0^{\circ}C$. The primary product of the decomposition process was m-Al2O3, which transformed to $\beta$"- or $\beta$-Al2O3 at temperature higher than 100$0^{\circ}C$. We found that the powder prepared at 120$0^{\circ}C$ had only $\beta$"- and $\beta$-Al2O3.EX>-Al2O3.

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Purification and characterization of β-secretase inhibitory peptide from sea hare (Aplysia kurodai) by enzymatic hydrolysis

  • Lee, Jung Kwon;Kim, Sung Rae;Byun, Hee-Guk
    • Fisheries and Aquatic Sciences
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    • v.21 no.5
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    • pp.13.1-13.8
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    • 2018
  • Amyloid plaque, also called senile plaque, the product of aggregation of ${\beta}$-amyloid peptides ($A{\beta}$), is observed in brains of the patients with Alzheimer's disease (AD) and is one of the key factors in etiology of the disease. In this study, hydrolysates obtained from the sea hare (Aplysia kurodai) were investigated for ${\beta}$-secretase inhibitory peptide. The sea hare's muscle protein was hydrolyzed using six enzymes in a batch reactor. Trypsin hydrolysate had highest ${\beta}$-secretase inhibitory activity compared to the other hydrolysates. ${\beta}$-secretase inhibitory peptide was separated using Sephadex G-25 column chromatography and high-performance liquid chromatography on a C18 column. ${\beta}$-secretase inhibitory peptide was identified as eight amino acid residues of Val-Ala-Ala-Leu-Met-Leu-Phe-Asn by N-terminal amino acid sequence analysis. $IC_{50}$ value of purified ${\beta}$-secretase inhibitory peptide was $74.25{\mu}M$, and Lineweaver-Burk plots suggested that the peptide purified from sea hare muscle protein acts as a competitive inhibitor against ${\beta}$-secretase. Results of this study suggest that peptides derived from sea hare muscle may be beneficial as anti-dementia compounds in functional foods or as pharmaceuticals.

Regulation of $\beta$-Galactosidase Biosynthesis in Alkalophilic, Thermophilic Bacillus sp. TA-11 (호알칼리성, 고온성 Bacillus sp. TA-11의 $\beta$-galactosidase의 생합성 조절)

  • Lee, Jong-Su;Lee, Hyang-Sook
    • The Journal of Natural Sciences
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    • v.5 no.2
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    • pp.13-17
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    • 1992
  • Regulation of $\beta$-galactosidase biosynthesis was studied with alkalophilic, thermophilic Bacillus sp. TA-11. Biosynthesis of the enzyme was effectively induced by lactose and some low level by isoprophyl-$\beta$-D-thiogalactopyranoside(IPTG). When 30mM glucose was added at the different intervals to the culture that had been in contact with lactose, the different levels of the enzyme synthesis were observed. So, this suggests that glucose interfered with the entry of the lactose into the cells.The glucose inhibitory effect was not relieved by adding cAMP to the culture.

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Synthesis of Hβ (core)/SAPO-11 (shell) Composite Molecular Sieve and its Catalytic Performances in the Methylation of Naphthalene with Methanol

  • Wang, Xiaoxiao;Guo, Shaoqing;Zhao, Liangfu
    • Bulletin of the Korean Chemical Society
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    • v.34 no.12
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    • pp.3829-3834
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    • 2013
  • $H{\beta}$ (core)/SAPO-11 (shell) composite molecular sieve was synthesized by the hydrothermal method in order to combine the advantages of $H{\beta}$ and SAPO-11 for the methylation of naphthalene with methanol. For comparison, the mechanical mixture was prepared through the blending of $H{\beta}$ and SAPO-11. The physicochemical properties of $H{\beta}$, SAPO-11, the composite and the mechanical mixture were characterized by various characterization methods. The characterization results indicated that $H{\beta}$/SAPO-11 composite molecular sieve exhibited a core-shell structure, with the $H{\beta}$ phase as the core and the SAPO-11 phase as the shell. The pore diameter of the composite was between that of $H{\beta}$ and SAPO-11. The composite had fewer acid sites than $H{\beta}$ and mechanical mixture while more acid sites than SAPO-11. The experimental results indicated that the composite exhibited high catalytic performances for the methylation of naphthalene with methanol.