• 한국치위생학회지
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• 제12권6호
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• pp.1203-1211
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• 2012

Objectives : The aim of this study was to investigate the factor-related oral care self-efficacy among the type 2 diabetic patients. Methods : Questionnaire was conducted with 174 Type 2 diabetic patients from 9th January to 9th March in 2012. The following conclusion was obtained as a result of carrying out t-test and one-way ANOVA analysis and multiple linear regression analysis. Results : 1. Analysis of the level of each item concerning oral care self-efficacy showed tooth brushing self-efficacy was $13.3{\pm}2.9$, the highest of all. 2. The factor that was most highly related with oral care self-efficacy was oral health behaviors(${\beta}=0.474$). The other factors were found to be expected duration of diabetes(${\beta}=-0.205$), self-assessed physical health(${\beta}=0.177$) and oral health(${\beta}=0.111$) in such order (p<0.05). Conclusions : Diabetes causes a variety of complications in the mouth, and therefore it is very important to practice oral care activity in order to oral health promotion. This study showed oral care self-efficacy appeared to be the greatest factor of relevance in practicing oral care activity. So, dental hygienist is obliged to keep on motivating so that the patient may maintain the oral care activity for him/herself. Also, a study on various intervention methods to improve oral care self-efficacy should be continued.

• 한국식생활문화학회지
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• 제28권2호
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• pp.186-194
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• 2013

The purpose of this study was to understand the effect of loyalty program in family and fastfood restaurant on relationship quality (trust, commitment) and revisit intention. Based on total 394 samples obtained from empirical research, this study reviewed the reliability and fitness of a research model using the Amos program. The hypothesized relationships in the model were simultaneously tested using a structural equation model (SEM). The proposed model provided an adequate fit to the data, ${\chi}^2$ 102.338 (df 48), GFI .959, RMR .049, NFI .970, CFI .984 SEM resulted that loyalty progam (${\beta}$= .429, t=7.880, p< .001) showed a positive significant effect on trust, and that loyalty program (${\beta}$= .455, t=9.532, p< .001) had a positive significant effect on commitment. Also, both trust (${\beta}$= .450, t=6.595, p< .001) and commitment (${\beta}$= .474, t=5.117, p< .001) had a positive significant effect on revisit intention. In addition, there were moderating effects related to restaurant type (family and fastfood restaurant) in terms of causal relationships between loyalty program, relationship quality and revisit intention. Limitations and future research directions are also discussed.

• 한국직업건강간호학회지
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• 제22권2호
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• pp.112-120
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• 2013

Purpose: The purpose of this study was to identify the influence of interpersonal attitude (I+, I-, U+, U-) on communication competence in care workers for frail elderly. Methods: This study was a cross-sectional survey. The data were collected from 153 care workers for frail elderly using a structured questionnaire. The data were analyzed using multiple regression with the SPSS/WIN 20.0 program. Results: The interpersonal attitude style of subjects was I+U+, and the score of communication competence was 3.53. There were significant differences in interpersonal attitude (I+, I-), and communication competence depending on age, education level, experience of personality type test (yes). Factors influencing on communication competence in care workers were interpersonal attitude (I+, I-, U+) and experience of personality type test with $R^2$ value of 48.5% (F=23.47 p<.001). The most influencing factor was I+ (${\beta}$=.36), followed by I- (${\beta}$=-.22), U+ (${\beta}$=.20), and experience of personality type test (yes) (${\beta}$=.16). Conclusion: It is needed to maintain the interpersonal attitude style (I+U+) of care workers. Continuing education program is needed for increasing communication competence especially for those fifties and over, and experienced care workers. Giving an opportunity for personality test is helpful to increase communication competence in care workers.

• 한국식품과학회지
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• 제27권4호
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• pp.631-634
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• 1995

계통이 다른 쌀 전분(일반계 3품종, 통일계 3품종)에서 열수 불용성 전분을 분리하고 그 전분의 구조적 성질을 조사하였다. 열수 불용성 전분의 아빌로오스 함량은 $2.7{\sim}6.1%$였다. 열수 불용성 전분의 사슬 분포는 ${\overline{DP}}\;55$ 이상의 부분이 $31.9{\sim}38.7%$, ${\overline{DP}}\;40{\sim}55$ 부분이 $12.3{\sim}18.0%$ 그리고 ${\overline{DP}}\;10{\sim}20$ 부분이 $48.5{\sim}50.4%$로서 각 품종별로 차이를 보였으며, ${\beta}$-한계 덱스트린의 분자 크기도 품종에 따라 차이를 보였다. 쌀의 열수 불용성 아밀로펙틴 분자 구조는 쌀 아밀로펙틴의 것과는 서로 달랐다. 이러한 결과는 쌀 전번의 열수 불용화는 각 품종에 따라 그 특성을 보이고, 아밀로펙틴의 분자 구조에 영향을 받고 있음을 알 수 있었다.

• Journal of Periodontal and Implant Science
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• 제49권4호
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• pp.258-267
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• 2019

Purpose: Increased bone regeneration has been achieved through the use of stem cells in combination with graft material. However, the survival of transplanted stem cells remains a major concern. The purpose of this study was to evaluate the viability of transplanted mesenchymal stem cells (MSCs) at an early time point (24 hours) based on the type and form of the scaffold used, including type I collagen membrane and synthetic bone. Methods: The stem cells were obtained from the periosteum of the otherwise healthy dental patients. Four symmetrical circular defects measuring 6 mm in diameter were made in New Zealand white rabbits using a trephine drill. The defects were grafted with 1) synthetic bone (${\beta}$-tricalcium phosphate/hydroxyapatite [${\beta}-TCP/HA$]) and $1{\times}10^5MSCs$, 2) collagen membrane and $1{\times}10^5MSCs$, 3) ${\beta}-TCP/HA+collagen$ membrane and $1{\times}10^5MSCs$, or 4) ${\beta}-TCP/HA$, a chipped collagen membrane and $1{\times}10^5MSCs$. Cellular viability and the cell migration rate were analyzed. Results: Cells were easily separated from the collagen membrane, but not from synthetic bone. The number of stem cells attached to synthetic bone in groups 1, 3, and 4 seemed to be similar. Cellular viability in group 2 was significantly higher than in the other groups (P<0.05). The cell migration rate was highest in group 2, but this difference was not statistically significant (P>0.05). Conclusions: This study showed that stem cells can be applied when a membrane is used as a scaffold under no or minimal pressure. When space maintenance is needed, stem cells can be loaded onto synthetic bone with a chipped membrane to enhance the survival rate.

• 미생물학회지
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• 제40권2호
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• pp.104-110
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• 2004

임상균주인 Enterobacter spp.가 생성하는 신규 chromosomal AmpC $\beta$-lactamases의 유전자형 및 진화적 측면을 고찰하기 위해서 항생제 감수성 시험, pI값 측정, DNA 염기서열 분석, 진화적 유연관계를 새로운 발현$.$분비 벡터를 이용하여 수행하였다. 6개 임상균주에서 cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin 및 amoxicillin-clavulanic acid에 내성 요인인 AmpC $\beta$-lactamase 유전자를 pMSG1219에 cloning하여 그 특성을 조사하였다. 381-amino-acid $\beta$-lactamase를 암호화 하는 4개의 ampC 유전자($bla_EcloK992004.1$, $bla_EcloK995120.1$, $bla_EcloK99230$ 및 $bla_EareK9911729$)는 E. cloacae MHN1의 chromosomal ampC 유전자($bla_EcloMHN1$)와 99.6% 이상의 상동성을 나타냈으며 두 ampC 유전자($bla_Eclok9973$ and $bla_EcloK9914325$)는 E. cloacae 908R의 chromosomal ampC 유전자($bla_EcloQ9908R$)와 99.7% 상동성을 나타냈다. 이런 결과는 6개 ampC 유전자가 $bla_EcloMHN1$ or $bla_EcloQ9908R$로부터 유래되었음을 시산한다. 발현ㆍ분비 벡터를 이용하여 제조한 6개 transformant의 MIC값 양상 및 정확한 pI값은 E. coli 균주에 외부 유전자의 특성을 고찰하는 목적에 개발된 발현$.$분비 백터(pMSG1219)가 유용함을 의미한다.

• 한국콘텐츠학회논문지
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• 제14권8호
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• pp.257-265
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• 2014

본 연구는 주관적 치주건강인식 수와 진료내용(임플란트, 교정진료, 일반진료)에 따른 행복지수를 파악하고, 구강보건측면의 영향요인을 파악함으로써 구강보건관리 프로그램을 개발하여 행복지수를 향상시킬 수 있는 기초자료를 제공하고자 한다. 부산.경남지역에 위치한 치과병 의원에 내원한 환자를 대상으로 2013년 3월부터 약 7개월간 총 400명을 대상으로 실시하여 불성실한 응답 16명을 제외하고 최종적으로 384명을 대상으로 하여 진료내용에 따른 행복지수는 단일문항(p<0.001), 5문항(p=0.037) 모두 교정치료를 받는 경우 높았고, 임플란트 진료를 받는 경우 낮았다. 주관적 치주건강인식 수에 따른 행복지수는 단일문항, 5문항 행복지수 모두 구강증상이 없는 경우 높았고(p<0.001), 주관적 건강상태와 주관적 구강건강 상태에서 매우 건강하다고 인식할수록 행복지수가 높았다(p<0.001). 단일문항 행복지수에 영향요인은 소득이 많을수록(${\beta}$=0.198), 주관적 건강상태가 좋을수록(${\beta}$=0.340) 행복지수가 높았으며, 당뇨질환이 있을수록(${\beta}$=-0.103), 치주질환수가 많을수록(${\beta}$=-0.204) 행복지수가 낮게 나타났다. 5문항 행복지수에 영향요인은 연령이 높을수록(${\beta}$=0.241), 소득이 많을수록(${\beta}$=0.198), 진료내용이 일반일 때(${\beta}$=0.107), 주관적 건강상태가 좋을수록(${\beta}$=0.275), 주관적 구강건강상태가 좋을수록(${\beta}$=0.132) 행복지수가 높았으며, 치주질환수가 많을수록(${\beta}$=-0.195) 행복지수가 낮았다. 병 의원에 내원한 환자를 대상으로 주관적 구강건강 인식을 향상하기 위한 구강건강프로그램이 마련되어야할 것이다.

• Childhood Kidney Diseases
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• 제15권2호
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• pp.125-137
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• 2011

목적: 일반적으로 남자는 여자에 비해 만성 신장병의 발병이 많고 말기 신부전으로의 진행이 더 흔한 것으로 알려져 있다. 본 연구는 일측성 요관 폐쇄를 가진 생쥐에서 신섬유화에 대한 성별과 성호르몬의 효과를 규명하기 위해 시행되었다. 방법: 일측성 완전 요관 폐쇄 7일째 암컷과 수컷 생쥐의 신장에서 ${\alpha}$-smooth muscle actin (${\alpha}$-SMA)의 발현을 측정한 후, 암컷 생쥐에서 난소를 제거하거나 제거 후 다시 $17{\beta}$-estradiol을 보충하여 나타나는 신섬유화 정도를 비교 분석하였다. 결과:일측성 요관 폐쇄를 가진 암컷 신장의 ${\alpha}$-SMA의 발현이 수컷 신장에 비해 현저히 낮았다. 난소 제거와 $17{\beta}$-estradiol의 보충은 일측성 요관 폐쇄를 가진 암컷 신장의 안지오텐신 II 1형 수용체의 발현에는 의미 있는 영향을 주지 않았지만, 안지오텐신 II 2형 수용체의 발현은 정상 암컷과 난소 제거 후 $17{\beta}$-estradiol를 보충한 암컷에서 현저히 증가되었다. 또한, inducible nitric oxide synthase (iNOS) 역시 유사한 변화를 보였다. 결론 : 여성은 폐쇄성 요로병증에서 신섬유화에 대한 저항성과 연관이 있으며 이러한 성별의 차이는 $17{\beta}$-estradiol에 의한 안지오텐신 II 2형 수용체와 iNOS의 발현 증가와 연관이 있을 것으로 사료된다.

• 한국발생생물학회지:발생과생식
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• 제22권2호
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• pp.155-163
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• 2018

This study aimed to investigate changes in the activity and mRNA expression of plasminogen activators (PAs) induced by $17{\beta}$-estradiol ($E_2$), human chorionic gonadotropin (hCG), and interleukin-$1{\beta}$ ($IL-1{\beta}$) in porcine endometrial cells. Endometrial cells were isolated from the epithelium and cultured to 80% confluence. They were then treated for 24 h with $E_2$ (0.2, 2, 20, and 200 ng/mL), $IL-1{\beta}$ (0.1, 1, 10, and 100 ng/mL), and hCG (0.5, 1, 1.5 and 2 IU/mL). mRNA expressions of urokinase-type (uPA) and tissue-type (tPA) PAs were analyzed using reverse transcription PCR, and activities were measured using a PA activity assay. mRNA expressions of uPA and tPA increased with $E_2$ treatment; however, this was not significant. Similarly, treatment with hCG did not influence the mRNA expressions of PAs. Interestingly, treatment with 0.1 ng/mL $IL-1{\beta}$ significantly reduced the mRNA expression of uPA, but did not affect that of tPA. Treatment with 2, 20, and 200 ng/mL $E_2$ increased PA activity compared with the control group; treatment with 0.1 and 1 ng/mL $IL-1{\beta}$ significantly increased PA activity compared with the other $IL-1{\beta}$ treatment groups, whereas treatment with 10 and 100 ng/mL $IL-1{\beta}$ decreased. Treatment with 2 IU/mL hCG increased PA activity compared with the other treatment groups, although there were no significant differences between the hCG and control groups. In conclusion, the activity and mRNA expression of PAs were differently regulated by the hormone/cytokine and its concentration in porcine endometrial cells. Therefore, understanding PA regulatory mechanisms may help to improve the reproductive potential of domestic animals.

• Journal of Microbiology and Biotechnology
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• 제16권6호
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• pp.889-895
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• 2006

To investigate the antibiotic-resistant patterns and the gene types of extended-spectrum $\beta$-lactamase (ESBL)-producing Klebsiella pneumoniae, we collected 226 Klebsiella pneumoniae strains from three general hospitals with more than 500 beds in Busan, Korea from September 2004 to October 2005, The minimum inhibitory concentration (MIC) of antibiotics was measured using the Gram-negative susceptibility (GNS) cards of Vitek (Vitek system, Hazelwood Inc., MO, U.S.A.). Of the 226 K, pneumoniae isolates, 65 ESBL-producing K. pneumoniae strains were detected by the Vitek system and confirmed by the double-disk synergy test. TEM (Temoniera) type, SHV (sulfhydryl variable) type, and CTX-M (cefotaxime) type genes were detected by polymerase chain reaction. All 65 K. pneumoniae strains were resistant to ampicillin, cefazolin, cefepime, ceftriaxone, and aztreonam, and 83.0% of the organisms were resistant to ampicillin/sulbactam, 66.1% to tobramycin, 67.6% to piperacillin/tazobactam, 61.5% to ciprofloxacin, and 47.6% to trimethoprim/sulfamethoxazole, and 43.0% to gentamicin. TEM-type ESBLs (TEM-1 type, -52 type) were found in 64.6% (42 of 65) of the isolates, SHV-type ESBLs (SHV-2a type, -12 type, -28 type) in 70.7% (46 of 65) of isolates, and CTX-M-type ESBLS (CTX-M-15 type) in 45% (29 of 65) of isolates. Of the 65 ESBL-producing K. pneumoniae strains, two strains were found to harbor blaSHV-28, which were detected in Korea for the first time. Therefore, more investigation and research on SHV-28 are needed in order to prevent the ESBL type-producing K. pneumoniae from spreading resistance to oxyimino cephalosporin antibiotics.