• Journal of Plant Biotechnology
• /
• 제44권2호
• /
• pp.164-170
• /
• 2017

A rapid and efficient in vitro regeneration system was established for Hibiscus syriacus L. The successful regeneration protocol employs induction of shoot organogenesis on leaf, petiole, and root explants. Among the various plant growth regulators evaluated, thidiazuron (TDZ) was the most effective for inducing rapid shoot formation. Most efficient shoot regeneration frequency was obtained from Murashige and Skoog (MS) media containing 0.01 mg/L TDZ. Regeneration efficiency was highest in the roots, and lowest in the leaves. A combination of 0.01 mg/L TDZ with benzyladenine (BAP) markedly improved the frequency of shoot differentiation from the root (up to 98%) and petiole (up to 88%) explants. Furthermore, leaf and petiole explants showed the highest frequency of shoot induction in half-strength MS media containing 0.01 mg/L TDZ and 1.0 mg/L BAP, while root explants formed the greatest number of shoots when 0.01 mg/L TDZ and 0.1 mg/L BAP were added to half-strength MS media. Although the frequency of shoot differentiation from leaf explants was only 50%, the leaf is considered the most efficient plant organ for use in tissue culture because leaves are easier to obtain than roots and petioles. Our findings show that various organs of H. syriacus can be used for plant regeneration, and the protocol developed in this study may be applicable in the horticulture industry.

• KSBB Journal
• /
• 제7권2호
• /
• pp.85-91
• /
• 1992

Hybrid Poplar(Poplar nigra var. betulifollia X Poplar trichorcarpa)의 잎절편을 목본실물 배양배지에서 배양하면서 기관분화를 위한 배양조건과 분화과정 중 부정아의 분화와 auxib(NAA)과 cytokinin(BA)과의 상호관계에 대해서 연구하여 다음고 같은 결과를 얻었다. 1. Poplar잎절편으로부터 부정아의 분화는 식물호르몬의 조성이 NAA $0.01mg/\ell$, BA$0.2mg/\ell$일 때 배양후 13일정도에서 기부 절단면으로부터 부정아가 효과적으로 유도되었다. 2. RNA와 단백질의 함량은 단부가 기부보다 높았으며 기부가 4일에서 10일까지의 상대적이 수치에서는 단부보다 높았다. 3. RNA와 단백질의 함량은 식물호르몬의 조성이 NAA,$0.01mg/\ell$;BA,$0.2mg/\ell$일 때 배양후 7일정도에서 가장 높았다. 4. 표준 WPM에 조직 절편을 배양하였을 때 기부에서 배양시기가 경과함에 따라 특이한 단백질이 형성 되었다.

• Plant Biotechnology Reports
• /
• 제4권2호
• /
• pp.157-163
• /
• 2010

A protocol for in vitro propagation from pseudobulb sections of Lycaste armomatica (Graham ex Hook) Lindl., an ornamental and fragrant orchid, was developed. The effect of four cytokinins: kinetin (K), metatopolin (mT), $N^6$-benzyladenine (BA), and thidiazuron (TDZ), in equimolar concentrations, was investigated. Shoot formation from apical and basal pseudobulb sections was obtained in all treatments. A few medial sections cultured in media supplemented with BA formed protocorm-like bodies. Shoot formation was greater from the basal section than the apical, and mainly occurred in explants cultured in media containing TDZ. The highest average numbers of shoots per explant were achieved from basal sections cultured in media supplemented with TDZ at 4.4, 8.87 and 2.2 ${\mu}M$, forming on average 9.9, 8.6 and 7.3 shoots per explant, respectively. Since the medial pseudobulb section was the worst explant for propagation of L. aromatica, we recommend that pseudobulbs be divided into two sections; the basal half should be cultured in MS medium supplemented with TDZ at 4.4 ${\mu}M$ and the apical half with TDZ at 2.2 ${\mu}M$. Subculturing individual shoots in MS medium without plant growth regulators allows further development and rooting. A survival rate of more than 90% under greenhouse conditions was achieved. This research represents a direct contribution to the conservation and sustainable use of this valuable natural resource.

• 한국약용작물학회지
• /
• 제25권2호
• /
• pp.95-101
• /
• 2017

Background: Callus cultivation has the advantage of producing a large amount of tissue of a plant in a laboratory regardless of the environment, for extracting an active substance. In the present study, callus formation was induced in the leaves of the succulent plant Adenium obesum (Forssk.) Roem & Schult. After callus cultivation, anti-inflammatory activity tests were conducted, because leaves and stems of A. obesum have been reported to possess biological activity. Methods and Results: In order to induce callus formation, various concentrations of plant growth factors, such as kinetin, naphtha-leneacetic acid (NAA), 6-benzyladenine (BA), and indole-3-acetic acid (IAA) were added to MS solid medium. The maximum callus proliferation was induced by mixed medium consisting of NAA ($2mg/{\ell}$) and BA ($1mg/{\ell}$). In addition, an elicitor was added to the medium under optimal conditions for initiating suspension culture. After suspension culturing, the activities of the callus extracts were compared and analyzed. The cytotoxicity and anti-inflammatory activity tests revealed that the anti-inflammatory activity of the callus extract and the content of phenolic compounds were elevated after treatment of the callus culture with the elicitior. Conclusions: A. obesum callus might be considered as potential source of biologically active anti-inflammatory material.

• Journal of Plant Biotechnology
• /
• 제5권4호
• /
• pp.221-224
• /
• 2003

Leaf explants of Ixeris sonchifolia produced adventitious shoots at a frequency of 100% when cultured on MS medium supplemented with combinations of various concentrations of 6-benzyladenine (BA) (0.44, 4.44, or 8.87 ${\mu}M$) and 0.54 ${\mu}M$ NAA, or MS medium supplemented with 22.19 ${\mu}M$ BA and 2.69 ${\mu}M\;\alpha$-naphthaleneacetic acid (NAA) after four weeks of culture. Each explants (approximately $3{\times}6mm$) produced greater than 70 shoots at a combination of 0.44 ${\mu}M$ BA and 0.54 ${\mu}M$ NAA. Leaf explants produced shoots at a frequency of greater than 80% even at as low as 0.13 ${\mu}M$ BA as the sole growth regulator. Upon transfer to one-third strength MS with 0.54 ${\mu}M$ NAA, excised adventitious shoots were rooted at a frequency of 100%. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse. The competence of I. sonchifolia for plant regeneration via organogenesis appears to be greater than the competence of tobacco, currently the best model plant for organogenesis.

• Journal of Plant Biotechnology
• /
• 제36권2호
• /
• pp.137-143
• /
• 2009

An efficient system for high frequency plant regeneration was established through investigating various factors such as plant growth regulator combinations, explant types and ages, and addition of $AgNO_3$ influenced on shoot regeneration in Brassica juncea L. cv. BARI sarisha-10. Murashige and Skoog (MS) medium supplemented with 0.1 mg/L NAA (1-naphthaleneacetic acid) and 1 mg/L BA (6-benzyladenine) showed the maximum shoot regeneration frequency (56.67%) among the different combinations of NAA and BA. Explant type, explant age, and addition of $AgNO_3$ also significantly affected shoot regeneration. Of the four type of explants (cotyledon, hypocotyl, root, and leaf explants)- cotyledon explants produced the highest shoot regeneration frequency and hypocotyls explants produced the highest number of shoots per explant, whereas root explants did not produce any shoot. The cotyledonary explants from Four-day-old seedlings showed the maximum shoot regeneration frequency and number of shoots per explant. Shoot regeneration frequency increased significantly by adding $AgNO_3$ to the medium. Two mg/L $AgNO_3$ appeared to be the best for shoot regeneration with the highest shoot regeneration frequency (86.67%) and number of shoots per explant (7.5 shoots). Considerable variation in shoot regeneration from cotyledonay explants was observed within the B. juncea L. genotypes. The shoot regeneration frequency ranged from 47.78% for cv. Shambol to 91.11% for cv. Rai-5. In terms of the number of shoots produced per explant, B. juncea L. cv. Daulot showed the maximum efficiency. MS medium supplemented with 0.1 mg/L NAA showed the highest frequency of rooting. The regenerated plantlets were transferred to pot soil and grown to maturity in the greenhouse. All plants were fertile and morphologically identical with the source plants.

• 원예과학기술지
• /
• 제35권2호
• /
• pp.252-264
• /
• 2017

Tea is one of the most consumed beverages worldwide and the relatively high levels polyphenols is benefit for health. In this study, we developed an efficient system for proliferation of callus from 'Anji Baicha', a cultivar of tea (Camellia sinensis). Callus tissue was initially induced by culturing leaf explants on medium containing different plant growth regulators. For callus induction, thidiazuron (TDZ) was more effective than 2,4-dichlorophenoxyacetic acid (2,4-D), ${\alpha}-naphthalene$ acetic acid (NAA), and $N^6-benzyladenine$ (BA). The frequency of callus induction from leaf explants reached 90.21% on $1.0mg{\cdot}L^{-1}$ TDZ and the developed callus was reddish and friable. We also tested the effect of different concentrations of NAA, 2,4-D, indole 3-acetic acid (IAA), BA, and TDZ, alone and in combinations, on callus proliferation. Medium supplemented with TDZ in combination with IAA was suitable for callus proliferation and accumulation of tea polyphenols. The growth index value and tea polyphenol content of callus cultured on MS medium containing $0.5mg{\cdot}L^{-1}$ TDZ and $1.0mg{\cdot}L^{-1}$ IAA was maximally 1,351% and 23.24%, respectively, and the relative abundance of epicatechin was as high as 17.449%. We also measured the antioxidant activity of all samples and the callus with the highest tea polyphenol content also exhibited high potential radical scavenging activity.

• 식물조직배양학회지
• /
• 제21권1호
• /
• pp.1-6
• /
• 1994

멜론의 배발생 캘러스의 유도와 식물체 재분화 시스템을 확립하기 위해서 자엽과 배축의 절편을 여러 농도의 2,4-D 와 BA가 첨가된 MS 기본배지에 치상하여 관찰한 결과, 초기배양에서는 4 mg/L 2,4-D와 1 mg/L BA 그리고 2 mg/L 2,4-D+O.1 mg/L BA가 첨가된 배지에서 연한 노란색의 배발생 캘러스가 형성되었고, 위의 조건에서 형성된 캘러스로부터 배발생 캘러스는 0.5와 1 mg/L 2,4-D가 첨가된 동일배지에서 약 75%가 형성되었다. 그리고 생장조절제를 첨가하지 않은 기본배지에서 대부분의 체세포배는 식물체로 재분화되었으며, 두개의 자엽을 갖춘 정상적인 체세포배보다 비정상적인 나팔형의 자엽을 가진 것이 3배 높은 빈도로 나타났다. 이러한 체세포배들은 MS 기본배지에서 식물체로 발아되었으며 생육상에서 생장하여 견실한 열매를 맺었다.

• Journal of Plant Biotechnology
• /
• 제34권2호
• /
• pp.161-166
• /
• 2007

In vitro shoot regeneration and multiple shoot induction has been obtained from the stolen node explants in Eremochloa ophiuroides (Munro) Hack. The highest number of shoots ($10.66{\pm}0.21$) was observed from initial explants after one month culture duration on Murashige and Skoog (MS) medium containing 6-benzyladenine (BA: 0.5 mg/l). First generation shoot was excised and sub-cultured on the same fresh media for further multiplication of shoots. An enhanced number of second round shoots ($15.33{\pm}0.21$) was obtained compared to the initial culture media containing BA (0.5 mg/l). The number of shoots/stolon node was higher among all the concentrations of BA than kinetin (KN). In vitro regenerated shoots were successfully rooted in the phytohormone free MS medium. Plantlets generated with roots were transferred to pots containing compound mixture of soil and kept in green house conditions. Acclimatized plants showed 100% survival rate with normal morphology in green house conditions. The present study demonstrates the effect of explant and different plant growth regulators towards in vitro response in E. ophiuroides. Moreover, the study reveals the effect of cytokinin on induction of shoot number per stolen node explant in E. ophiuroides.

• Journal of Plant Biotechnology
• /
• 제46권4호
• /
• pp.291-296
• /
• 2019

본 연구는 티크의 기내 생장 및 발근에 미치는 light-emitting diode (LED) 효과를 구명하기 위해 수행되었다. 티크의 정아 절편체의 줄기 신장은 LED 혼합광(BR, 50% 청색광 + 50% 적색광) 하에서 DKW 배지 배양 시 3.2 cm로 가장 좋았고, 줄기유도는 광원 및 배지(MS 및 DKW) 효과가 그리 크지 않은 것으로 나타났다. BA 농도 처리 시 최대 줄기 유도는 BR 광원 및 1.0 mg/L 처리구에서 2.4개, 최대 줄기 신장은 BR 및 0.5 mg/L 처리구에서 4.94 cm로 가장 효과적이었다. 발근에 영향하는 IBA 농도 효과는 정아 절편체를 BR 및 0.5 mg/L 처리구에서 배양 시 최대 발근율(93.8%)과 최대 뿌리 길이 신장(1.3 cm)을 보였고, 뿌리 수는 0.2 mg/L (3.1개/절편) 처리구에서 가장 좋은 것으로 나타났다. 발근 식물체는 상토로 이식 후 순화 과정을 거쳐 정상적은 생장을 보였다.