• 분석과학
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• 제17권1호
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• pp.45-52
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• 2004

현이차 미분 형광 분광광도법을 이용하여 울산만 해양 저질토양중의 PAHs를 n-hexane용매로 추출하여 11종의 PAHs를 동시 정량분석하였다. Acenaphthene (Ace), anthracene (Anth), benzo(a)pyrene (BaP), benz(a)anthracene (BaA), benzo(b)fluoranthene (BbFt), benzo(k)fluoranthene (BkFt), chrysene (Chry), perylene (Per), phenanthrene (Phen), pyrene (Pyr) 및 fluoranthrene (Ft) 등을 정량분석 하였다. 이들 성분들의 검정선은 대략 0.15~166 ppb의 농도범위에서 직선관계를 보였으며, 0.999이상의 좋은 직선 상관계수를 보였다. 울산만의 해양 저질 토양 (sediment)에 함유된 11종의 PAHs 총량은 68.8 ng/g ~ 324.4 ng/g의 농도범위로 함유되어 있었다. 또한 PAHs의 총량은 울산만의 안쪽으로 갈수록 증가하는 경향을 보였으며, 특히 Pyr과 BaA 등과 같은 4고리화합물의 함량비가 높았다.

• 한국식품영양과학회지
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• 제27권4호
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• pp.712-717
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• 1998

This study was undertaken to investigate the inhibition effects of Auricularia auricula-judae methanol extract in edible mushroom on lipid peroxidation and liver damage in benzo(a)pyrene(B(a)P)-treated mice. The activities of serum aminotransferase, cytochrome P-450, superoxide dismutase, catalase, glutathione peroxidase and the hepatic content of lipid peroxide after B(a)P-treatment was markedly increased than control but those levels were significantly decreased by the treatment of Auricularia auricula-judae methanol extract. Glutathione S-transferase activity and the hepatic glutathione content were decreased by B(a)P-treatment than control, but those were also inhibited by the treament of Auricularia auricula-judae methanol extract. These results suggest that Auricularia auricula-judae methanol extract have a protective effect on liver damage by B(a)P.

• Environmental Analysis Health and Toxicology
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• 제7권1_2호
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• pp.35-43
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• 1992

Mugwort has been used as a Korean folk medicine in treating liver diseases acting as an analgesics, sedative, diuresis, choleretics. This study was perfomed to evaluate the effect of mugwort extracts on the changes of enzyme activities, lipid accumulation of the serum and liver, when hepatotoxicity was induced by benzo(a)pyrene. The results are as follows: 1. Mugwort water extract administration prevented the increase of serum and liver AST, ALT, LDH, ${\gamma}$-GTP, liver ALP activities and bilirubin content caused by B(a)P injection. 2. The increase of serum and liver ALT, LDH, ${\gamma}$-GTP, serum AST activities and liver bilirubin contents in B(a)P treated group were decreased by mugwort methanol extract treatment. 3. Serum and liver total cholesterol, phospholipid, triglyceride level and serum HDL-cholesterol level were increased by B(a)P treatment. After combined treatment of mugwort water and methanul extracts, these lipid content were significantly decreased. 4. The hepatotropic effect of mugwort water extract and after-treatment against B(a)P induced hepatotoxicity was superior to that of methanol extract and pretreatment.

• 약학회지
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• 제34권5호
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• pp.291-295
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• 1990

A modified method was investigated for the determination of benzo(a)pyrene metabolites generated by the rat liver microsomes based on the HPLC technique with radioactive flow detection. By adding $[^3H]-dexamethasone$ to the B(a)P metabolites mixture metabolized by the microsome, the poor yield of solvent extaction of B(a)P metabolites was compensated.

• Environmental Analysis Health and Toxicology
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• 제7권1_2호
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• pp.45-51
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• 1992

This study was performed to investigate the effect of Angelicae gigantis Radix extract oil the hepatic detoxifying enzyme activities and lipids. Male sprague-dawley rats were administrated the extracts with benzo(a)pyrene, a hepatotoxic agent, inducing liver damages. Results obtained from this study were as follows: 1. The markedly increased enzyme activities (AST, ALT, LDH, ALP, ${\gamma}$-GTP, GSH-Px) in B(a)P induced groups tended to be decreased by the treatment of the Angelicae gigantis Radix extract. 2. Liver GSH content and lipid peroxide activity were decreased by the administration of the extracts. 3. It tended that the curative effects were better than the protective effects of the extracts.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1984년도 학술대회지
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• pp.27-36
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• 1984

홍삼의 항발암작용 기전을 규명하기 위한 목적의 일환으로 홍삼추출물이 urethan 및 b두캐 (a) pyrene을 투여한 마우스에서 natural killer(N.K) 세포활성도 및 폐선종 발생에 미치는 영향을 발암물질 처리 후 48주동안 검색하였다. N.K 세포 활성도는 urethan 및 benzo (a) pyrene 의 처리에 의하여 현저히 저하되었다. 이와 같은 N. K 세포활성도의 저하는 상기 발암물질 투여후 4주부터 나타나 24주까지 계속되었으며 홍삼투여에 의하여 정상대조군의 수준으로 되돌아 왔다. 동시에 urethan에 의해 6주시부터 유발되기 시작한 폐선종 역시 홍삼투여에 의해 그 발생빈도가 현저히 억제되었다. 반면 benzo (a) pyrene에 의해 유발된 폐선종은 48주시에 나타나기 시작하였는데 이 시기는 N.K 세포 활성도가 자연적으로 너무 낮은 수준으로 떨어져 있어 홍삼의 영향을 받을 수 없는 시기였으며 홍삼투여에 의해 폐선종의 발생빈도 역시 억제되지 않았다. 결론적으로 저자들은 본 연구를 통하여 홍삼에 의한 항발암 효과는 N.K 세포활성도의 증대와 관련되어 있음을 알 수 있었다.

• 한국자기공명학회논문지
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• 제26권4호
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• pp.59-65
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• 2022

Benzo[a]pyrene (BaP), one of the polycyclic aromatic hydrocarbons (PAHs), is an endocrine disruptor and carcinogenic. This study was conducted to investigate the metabolic changes of zebrafish short-term exposure to BaP using nuclear magnetic resonance (NMR) spectroscopy. In our results, the multivariate analysis showed that the metabolic responses were differed according to the exposure concentration. Also, it was observed that exposure to high concentration of BaP (162 ㎍/L and 1620 ㎍/L) increased the levels of creatine, histidine, and inosine in zebrafish, which means high concentration of BaP exposure affected the energy metabolism and immune function in zebrafish.

• 한국식품과학회지
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• 제49권2호
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• pp.162-167
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• 2017

훈연 가쓰오부시의 벤조피렌 저감화를 위하여 훈연기를 개선하고 이의 저감화 효과를 평가하였다. 기존 훈연기는 동일한 히터를 사용하여 연기생성 및 가열을 동시에 행하는데 비하여 개선된 훈연기는 연기생성부 및 가열부를 갖추어 연기생성과 가열이 각각 되도록 하였다. 연기생성부는 훈연실 내부에 설치된 CdS 광센서, on/off 제어기, 연소실과 훈연실 사이에 연기 공급을 개폐하는 압축구동 회전체로 구성하였다. 가열부는 훈연실 내부의 온도센서, PID 제어기, 전기히터로 구성하였다. 자숙된 가다랑어를 연기농도를 세가지 수준으로 제어하며 45도에서 훈연한 결과 108시간 경과 후 연기농도가 낮은 순으로 벤조피렌 농도가 5.87, 7.83, $11.41{\mu}g/kg$로 각각 나타났다. 연기농도를 가장 낮은 농도에서 온도를 달리하여 훈연한 결과 벤조피렌 농도가 각각 5.87, 4.82, $3.27{\mu}g/kg$로 검출되었다. 따라서 벤조피렌 저감화 조건은 낮은 연기농도와 높은 온도가 바람직함을 알 수 있었다. 기존 훈연기는 높은 온도를 사용할 경우 연기농도가 종속적으로 높아지기 때문에 저감화 조건을 만족시킬 수 없는 반면에 본 개선된 훈연기는 저감화를 구현할 수 있었다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.6159-6164
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• 2013

Background: Phytochemical compounds are emerging as a new generation of anticancer agents with limited toxicity in cancer patients. The purpose of this study was to investigate the potential effcts of thymoquinone, caffeic acid phenylester (CAPE) and resveratrol on inflammatory markers, oxidative stress parameters, mRNA expression levels of proteins and survival of lung cancer cells in Vitro. Materials and Methods: The A549 cell line was treated with benzo(a)pyrene, benzo(a)pyrene plus caffeic acid phenylester (CAPE), benzo(a)pyrene plus resveratrol (RES), and benzo(a)pyrene plus thymoquinone (TQ). Inflammatory markers, oxidative stress parameters, mRNA expression levels of apoptotic and anti-apoptotic proteins and cell viability were assessed and results were compared among study groups. Results: TQ treatment up-regulated Bax and down-regulated Bcl2 proteins and increased the Bax/Bcl2 ratio. CAPE and TQ also up-regulated Bax expression. RES and TQ down-regulated the expression of Bcl-2. All three agents decreased the expression of cyclin D and increased the expression of p21. However, the most significant up-regulation of p21 expression was observed in TQ treated cells. CAPE, RES and TQ up-regulated TRAIL receptor 1 and 2 expression. RES and TQ down-regulated the expression of NF-kappa B and IKK1. Viability of CAPE, RES and TQ treated cells was found to be significantly decreased when compared with the control group (p=0.004). Conclusions: Our results revealed up-regulation of the key upstream signaling factors, which ultimately cause increase in their regulatory p53 levels affecting the induction of G2/M cell cycle arrest and apoptosis. Overall these results provide mechanistic insights for understanding the molecular basis and utility of the anti-tumor activity of TQ, RES and CAPE.

• 한국환경성돌연변이발암원학회지
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• 제18권2호
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• pp.71-76
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• 1998

환경독성물질에 의한 폭로는 세포내 DNA의 수복과정에 영향을 미쳐 돌연변이나 암을 유발할 수 있다. 독성물질에 의해 유도된 DNA의 비정상 수복효과를 판단하기 위하여.in vivo에서 MNNG 또는 Benzo(a)pyrene을 투여하고 in vitro에서 Bleomycin을 처리하여 나타나는 염색체이상효과를 관찰하였다. 실험결과, MNNG를 투여 후 Bleomycin을 처리하였을 때 염색체이상의 상승효과가 나타났다. 한편, Benzo(a)pyrene 투여 후 Bleomycin을 처리하였을 패는 높은 농도에서 염색체이상의 상승효과가 나타났다. 이같은 결과는 MNNG나 Benzo(a)pyrene 같은 유전독성물질들이 in vivo에서 세포내 비정상 DNA수복을 일으킬 수 있으며, 이러한 작용은 관련 유전독성물질의 염색체손상성에 무관하며 투여용량에 의존되는 것으로 판단된다.