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Analysis of the Amino Acids Content of Three Neopyropia Dentata Cultivars under the Two Different Aquafarm Environment in Haenam, Korea (해남의 김 양식장별 잇바디돌김(Neopyropia dentata) 3품종의 아미노산 성분 분석)

  • Hye Ri Nam;Sung Je Choi
    • Journal of Practical Agriculture & Fisheries Research
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    • v.25 no.3
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    • pp.5-13
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    • 2023
  • This study aims to assess the water quality of the Eoran and the Imha aquafarm with different aquafarm environments in Haenamn-gun, and analyzed the composition of total amino acids (TAAs) and free amino acids (FAAs) in three Neopyropia dentata cultivars (Yuldo, Supum1 and 2) in two aquafarms. Mean water temperature ranged from 22.9 to 10.9℃ during September to November 2018. In Eoran aquafarm, the water quality analysis showed that NO2-N was high in September, NH4-N and COD in October, and NO3-N, DIN, and DIP in November. In Imha aquafarm, the water quality analysis showed that NH4-N and COD was high in September, NO3-N and DIN in October, and NO2-N and DIP in November. We confirmed the proximate composition, amino acid composition (TAA/FAA) in two auqufarms. In the Eoran aquafarm, the 'Yuldo' cultivar had significantly higher crude lipid content than two other cultivars (Supum 1 and 2). The 'Supum1' cultivar had significantly higher moisture content, whereas the highest content of crude protein, crude lipid, crude ash, and carbohydrates was found in the 'Supum2' cultivar. In the Imha aquafarm, the content of crude lipid and crude ash was highest in the 'Yuldo' cultivar. The highest content of crude protein and carbohydrates was found in the 'Supum1' cultivar, while the 'Supum2' cultivar had the highest content of moisture. The highest concentration of glutamic acid belong to TAAs is observed in all cultivars from Eoran and Imha aquafarm, while all cultivars in two aquafarm also contained higher content of alanine among the detected FAAs.

Isolation and Characterization of 𝛽-Glucosidase-Producing Yeast, Rhodotorula sp. GYP-1 (𝛽-Glucosidase 생성 효모 Rhodotorula sp. GYP-1의 분리 및 특성)

  • Hyun-Soo Roh;Min-Young Kwon;Sol-Bi Kim;Jae-Eun Cho;Song-Ih Han
    • Journal of the Korean Applied Science and Technology
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    • v.40 no.5
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    • pp.1126-1135
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    • 2023
  • Nine microbial strains were isolated from the byproduct of ginseng processing and field of ginseng cultivation. Two strains among them were confirmed. Phylogenetic analysis of these 𝛽-Glucosidase strains confirmed that strain GYP-1 belongs to the Rhodotorula and strain GYP-3-3 belong to genus Brachybacterium. Rhodotorula sp. GYP-1 was finally selected due to its high biomass production. The 𝛽-Glucosidase activity of Rhodotorula sp. GPY-1 was assessed at 30 ℃, and Higher than 70% of the enzyme activity was maintained at the temperature range of 20-40℃. Although the optimum pH for the highest enzyme activity was pH 5.0, the enzyme was stable throughout the pH range of 5.0-8.0. In addition, Rhodotorula sp. demonstrated antifungal activity against the ginseng root rot disease caused by Botrytis.

A Social Psychology of the Afterlife Views (내세관의 사회심리학: 일상생활과의 관련성을 중심으로)

  • Yoshiyuki Inumiya;Il-Ho Choi;Seong-Yeul Han
    • Korean Journal of Culture and Social Issue
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    • v.8 no.1
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    • pp.141-164
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    • 2002
  • The present study examined the variations in personality variables according to different types for the Korean view of the afterlife, including 'extinction type', 'the other world-oriented transmigration type', 'heaven/hell type' and 'obscurity type'. The results may be summarized as follows: (1) 'the other world-oriented transmigration type' and 'heaven/hell type' showed significantly stronger belief in a just world than 'extinction type', (2) 'extinction type' and 'obscurity type' showed more external attribution for accidents than 'heaven/hell type', (3) there was no difference in unrealistic optimism levels, (4) depending upon the types for the view of the afterlife, not only preferred self-enhancing biases but also positive illusions which related to life satisfaction were different, (5) 'extinction type' showed stronger risk-taking tendency than 'heaven/hell type'. These results were explained in terms of features that belong to each afterlife view. Finally, we discussed the limitation of this study and the directions for future study.

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An Empirical Study on KOSDAQ-Listed SMEs' Convertible Bonds and Financial Constraints (코스닥 기업의 전환사채 발행이 금융제약에 미치는 영향에 관한 실증연구)

  • Binh, Ki Beom;Byun, Jinho;Park, Kyung Hee
    • Korean small business review
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    • v.42 no.3
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    • pp.173-193
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    • 2020
  • This study analyzes the effects of KOSDAQ-listed firms' convertible bonds, which have recently increased rapidly in number and size. Although KOSDAQ companies are called mid-size companies, KOSDAQ companies belong to SMEs. Furthermore, convertible bonds have traditionally been a critical capital raising tool for SMEs in the US and Europe. In Korea, KOSDAQ companies actively employ convertible bonds. Convertible bonds provide investment incentives for hesitant investors, allowing companies to raise capital at low interest rates. This study analyzes whether capital raising through issuance of convertible bonds by KOSDAQ companies affects their financial constraints. Financial constraints result from incomplete capital markets, which are embedded in most companies and countries.. In particular, financial constraints have a significant impact on the growth and survival of SMEs. The seminal study FHP(1988) is the most important and effective study of firm's financial constraints. We find that FHP's financial constraint measures show that convertible bond issuance would mitigate the financial constraints of KOSDAQ companies. However, the significance of the evidence is not strong.

Comparison of miR-106b, miR-191, and miR-30d expression dynamics in milk with regard to its composition in Holstein and Ayrshire cows

  • Marina V. Pozovnikova;Viktoria B. Leibova;Olga V. Tulinova;Elena A. Romanova;Artem P. Dysin;Natalia V. Dementieva;Anastasiia I. Azovtseva;Sergey E. Sedykh
    • Animal Bioscience
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    • v.37 no.6
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    • pp.965-981
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    • 2024
  • Objective: Milk composition varies considerably and depends on paratypical, genetic, and epigenetic factors. MiRNAs belong to the class of small non-coding RNAs; they are one of the key tools of epigenetic control because of their ability to regulate gene expression at the post-transcriptional level. We compared the relative expression levels of miR-106b, miR-191, and miR-30d in milk to demonstrate the relationship between the content of these miRNAs with protein and fat components of milk in Holstein and Ayrshire cattle. Methods: Milk fat, protein, and casein contents were determined in the obtained samples, as well as the content of the main fatty acids (g/100 g milk), including: saturated acids, such as myristic (C14:0), palmitic (C16:0), and stearic (C18:0) acids; monounsaturated acids, including oleic (C18:1) acid; as well as long-, medium- and short-chain, polyunsaturated, and trans fatty acids. Real-time stem-loop one-tube reverse transcription polymerase chain reaction with TaqMan probes was used to measure the miRNA expression levels. Results: The miRNA expression levels in milk samples were found to be decreased in the first two months in Holstein breed, and in the first four months in Ayrshire breed. Correlation analysis did not reveal any dependence between changes in the expression level of miRNA and milk fat content, but showed a multidirectional relationship with individual milk fatty acids. Positive associations between the expression levels of miR-106b and miR-30d and protein and casein content were found in the Ayrshire breed. Receiver operating characteristic curve analysis showed that miR-106b and miR-30d expression levels can cause changes in fatty acid and protein composition of milk in Ayrshire cows, whereas miR-106b expression level determines the fatty acid composition in Holsteins. Conclusion: The data obtained in this study showed that miR-106b, miR-191, and miR-30d expression levels in milk samples have peculiarities associated with breed affiliation and the lactation period.

Speaker verification with ECAPA-TDNN trained on new dataset combined with Voxceleb and Korean (Voxceleb과 한국어를 결합한 새로운 데이터셋으로 학습된 ECAPA-TDNN을 활용한 화자 검증)

  • Keumjae Yoon;Soyoung Park
    • The Korean Journal of Applied Statistics
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    • v.37 no.2
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    • pp.209-224
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    • 2024
  • Speaker verification is becoming popular as a method of non-face-to-face identity authentication. It involves determining whether two voice data belong to the same speaker. In cases where the criminal's voice remains at the crime scene, it is vital to establish a speaker verification system that can accurately compare the two voice evidence. In this study, to achieve this, a new speaker verification system was built using a deep learning model for Korean language. High-dimensional voice data with a high variability like background noise made it necessary to use deep learning-based methods for speaker matching. To construct the matching algorithm, the ECAPA-TDNN model, known as the most famous deep learning system for speaker verification, was selected. A large dataset of the voice data, Voxceleb, collected from people of various nationalities without Korean. To study the appropriate form of datasets necessary for learning the Korean language, experiments were carried out to find out how Korean voice data affects the matching performance. The results showed that when comparing models learned only with Voxceleb and models learned with datasets combining Voxceleb and Korean datasets to maximize language and speaker diversity, the performance of learning data, including Korean, is improved for all test sets.

Genetic Diversity and Molecular Phylogenetic Relationships of the Genus Sarcocheilichthys Fish in Korea (한국산 중고기속(Sarcocheilichthys) 어류의 유전적 다양성과 분자계통학적 유연관계)

  • Ji-Wang Jang;Jae-Goo Kim;Jae-Geun Ko;Bong-Han Yun;Yang-Seop Bae
    • Korean Journal of Ichthyology
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    • v.36 no.2
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    • pp.139-155
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    • 2024
  • Using the cytb gene region of the mitochondrial DNA of eight populations of Sarcocheilichthys nigripinnis morii and five populations of S. variegatus wakiyae, which belong to the genus Sarcocheilichthys from Korea, the genetic diversity and molecular phylogenetic relationships of each population were examined. As a result of the analysis, it was confirmed that the S. variegatus wakiyae population had higher genetic diversity than the S. nigripinnis morii population. In the phylogenetic tree of genus Sarcocheilichthys fish in Korea based on the cytb gene, the Yeongsan River (YSR) population of S. variegatus wakiyae forms a clade with the Tamjin River (TJR), Yeongsan River (YSR), and Seomjin River (SJR) population of S. nigripinnis morii, and genetic relationships that do not align with the current classification system were observed. Meanwhile, on the nuclear DNA phylogenetic tree, S. variegatus wakiyae and S. nigripinnis morii could be clearly distinguished, showing mitonuclear inconsistency where mitochondrial and nuclear DNA conflicted on the phylogenetic tree. The Seomjin River (SJR) population of S. nigripinnis morii was translocated to the Dongjin River (DJR) population, haplotype from which crossbreeding was presumed to have occurred was confirmed. Among the rivers flowing into the East Sea, the S. nigripinnis morii population is known to have been introduced and inhabit only the Hyeongsan River (HSR), and it is presumed to be a population formed by translocation from the Han River (HR) population, with a haplotype representing a unique genetic group also confirmed. The Han River (HR), Geum River (GR), and Mangyeong River (MGR) populations of S. nigripinnis morii formed a genetically identical population with S. czerskii and S. soldatovi distributed north of the Yalu River, and accordingly, a taxonomic reexamination was required through morphological and molecular phylogenetic studies by securing various specimens.

Pulsatility Index in Different Modifications of Fontan Palliation: An Echocardiographic Assessment

  • Reza Shabanian;Parvin Akbari Asbagh;Abdullah Sedaghat;Minoo Dadkhah;Zahra Esmaeeli;Aliyeh Nikdoost;Manizheh Ahani;Mitra Rahimzadeh;Alireza Dehestan;Mohammad Ali Navabi
    • Journal of Cardiovascular Imaging
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    • v.30 no.2
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    • pp.99-108
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    • 2022
  • BACKGROUND: Adding pulsation to the Fontan circulation might change the fate of patients palliated by this procedure. Our aim was to compare the pulsatility index (PI) of the pulmonary artery (PA) between the various modifications of Fontan palliation. METHODS: Doppler-derived PI was measured in PA branches of a cohort of 28 patients palliated by 6 modifications of Fontan procedure. A group of normal individuals was included for comparison. RESULTS: Atriopulmonary connection (APC) group had the highest PA branches PI and statistically was close to the PI of the normal individuals (right pulmonary artery [RPA] PI of 1.58 vs. 1.63; p = 0.99 and left pulmonary artery [LPA] PI of 1.54 vs. 1.68; p = 0.46, respectively). The lowest PA branches PI was seen in the group of extracardiac total cavopulmonary connection (RPA PI of 0.62 and LPA PI of 0.65). Other 4 modifications including the extracardiac conduit with oversewn pulmonary valve, extracardiac conduit with preserved adjusted antegrade flow, extracardiac conduit from inferior vena cava onto the rudimentary right ventricle and lateral tunnel had a mean "RPA and LPA" PI of "1.19 and 1.17", "1.16 and 1.11", "1.13 and 1.11", "0.82 and 0.84", respectively. The modified Dunnett's post hoc test has shown a significant statistical decline in PI of all modifications compared to the normal individuals except for the APC group. CONCLUSIONS: Fontan palliated patients in different groups of surgical modification showed a spectrum of Doppler-derived PI with the highest amounts belong to the groups of pulsatile Fontan.

The Impacts of Social Support and Psychological Factors on Guild Members' Flow and Loyalty in MMORPG (MMORPG에서 길드 구성원들의 사회적 지지와 심리적 요인들이 플로우 및 충성도에 미치는 영향)

  • Kang, Ju-Seon;Ko, Yoon-Jung;Ko, Il-Sang
    • Asia pacific journal of information systems
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    • v.19 no.3
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    • pp.69-98
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    • 2009
  • We investigated what factors motivate gamers to participate in a guild and why they continue to be engaged as members of the guild. We find that, based on the result of focus group interviews with MMORPG gamers, social support and self-esteem factors play important roles. Considering both prior research and the focus group interviews we have conducted, we define social support and character control as independent variables. Character identity, guild identity, and self-esteem are proposed as mediating variables while guild flow and game loyalty as dependent variables. Accordingly, we develop the research model and hypotheses, and verify them empirically. Based on our experiences of playing the WoW game, we proposed a research model and conducted focus-group interviews (FGIs). FGIs involve formulating a hypothesis and then collecting some relevant data. FGIs were conducted face-to-face with students of C University in Korea. We formulated structured interview schedules, and the questions were based on our research variables and personal experiences. The questions for the interviews encompassed the following areas: (a) the demographic characteristics of the focus group; (b) the number of years for which respondents had played online games; (c) the motive for starting a game; (d) the number of game-characters assumed by each gamer; (e) the type of game played; and (f) other issues such as the reasons for involvement in the play, the willingness to reuse the game in case new versions were released, etc. On average, it took two hours to interview each of three groups. A primary set of FGIs was conducted with three groups on the premise that there would be some differences caused by character race (Horde vs. Alliance) or by playable server (Normal vs. Combat). With respect to the manner of playing, we found that guild members shared information, felt a sense of belonging, and played computer games for quite a long time through the guild; however, they did not undergo these experiences when playing alone. Gamers who belonged to a specific guild helped other players without expecting compensation for that, freely shared information about the game, gave away items for free, and more generous with other members who made mistakes. The guild members were aware of the existence other members and experienced a sense of belonging through interactions with, and evaluations from, other players. It was clear that social support was shown within the guild and that it played an important role as a major research variable. Based on the results of the first FGIs, a second set of in-depth FGIs was carried out with a focus on the psychology of the individual within the guild and the social community of the guild. The second set of FGIs also focused on the guild's offline meetings. Gamers, over all, recognize the necessity of joining a community, not only off-line but also online world of the guild. They admit that the guild is important for them to easily and conveniently enjoy playing online computer games. The active behavior and positive attitudes of existing guild members can motivate new members of the guild to adapt themselves to the guild environment. They then adopt the same behaviors and attitudes of established guild members. In this manner, the new members of the guild strengthen the bonds with other gamers while feeling a sense of belonging, and developing social identity, thereby. It was discovered that the interaction among guild members and the social support encouraged new gamers to quickly develop a sense of social identity and increase their self-esteem. The guild seemed to play the role of socializing gamers. Sometimes, even in the real world, the guild members helped one another; therefore, the features of the guild also spilled over to the offline environment. We intend to use self-esteem, which was found through the second set of FGIs, as an important research variable. To collect data, an online survey was designed with a questionnaire to be completed by WoW gamers, who belong to a guild. The survey was registered on the best three domestic game-sites: 'WoW playforum,' 'WoW gamemeca,' and 'Wow invent.' The selected items to be measured in the questionnaire were decided based on prior research and data from FGIs. To verify the content of the questionnaire, we carried out a pilot test with the same participants to point out ambiguous questions as a way to ensure maximum accuracy of the survey result. A total of 244 responses were analyzed from the 250 completed questionnaires. The SEM analysis was used to test goodness-of-fit of the model. As a result, we found important results as follows: First, according to the statistics, social support had statistically significant impacts on character control, character identity, guild identity and self-esteem. Second, character control had significant effects on character identity, guild identity and self-esteem. Third, character identity shows its clear impact on self-esteem and game loyalty. Fourth, guild identity affected self-esteem, guild flow and game loyalty. Fifth, self-esteem had a positive influence on the guild flow. These days, the number of virtual community is rising along with its significance largely because of the nature of the online games. Accordingly, this study is designed to clarify the psychological relationship between gamers within the guild that has been generally established by gamers to play online games together. This study focuses on the relationships in which social support influences guild flow or game loyalty through character control, character identity, guild identity, and self-esteem, which are present within a guild in the MMORPG game environment. The study results are as follows. First, the effects of social support on character control, character identity, guild identity and self-esteem are proven to be statistically significant. It was found that character control improves character identity, guild identity and self-esteem. Among the seven variables, social support, which is derived from FGIs, plays an important role in this study. With the active support of other guild members, gamers can improve their ability to develop good characters and to control them. Second, character identity has a positive effect on self-esteem and game loyalty, while guild identity has a significant effect on self-esteem, guild flow and game loyalty. Self-esteem affects guild flow. It was found that the higher the character and guild identities become, the greater the self-esteem is established. Contrary to the findings of prior research, our study results indicate that the relationship between character identity and guild flow is not significant. Rather, it was found that character identity directly affects game players' loyalty. Even though the character identity had no direct effect on increasing guild flow, it has indirectly affected guild flow through self-esteem. The significant relationship between self-esteem and guild flow indicates that gamers achieve flow, i.e., a feeling of pleasure and excitement through social support. Several important implications of this study should be noted. First, both qualitative and quantitative methods were used to conduct this study. Through FGIs, it was observed that both social support and self-esteem are important variables. Second, because guilds had been rarely studied, this research is expected to play an important role in the online community. Third, according to the result, six hypotheses (H1, H5, H6, H7, H8, and H11) setup based on FGIs, were statistically significant; thus, we can suggest the corresponding relationships among the variables as a guideline for follow-up research. Our research is significant as it has following implications: first, the social support of the guild members is important when establishing character control, character identity, guildidentity and self-esteem. It is also a major variable that affects guild flow and game loyalty. Second, character control when improved by social support shows notable influence on the development of character identity, guild identity and self-esteem. Third, character identity and guild identity are major factors to help establish gamers' own self-esteem. Fourth, character identity affects guild flow through self-esteem and game loyalty. The gamers usually express themselves through characters; the higher character identity is, the more loyalty a gamer has. Fifth, guild identity, established within the guild, has clear effects on self-esteem, guild flow and game loyalty. Sixth, qualitative and quantitative methods are employed to conduct this study. Based on the results of focus group interviews and SEM analysis, we find that the social support by guild members and psychological factors are significant in strengthening the flow of guild and loyalty to the game. As such, game developers should provide some extra functions for guild community, through which gamers can play online games in collaboration with one another. Also, we suggest that positive self-esteem which is built up through social support can help gamers achieve higher level of flow and satisfaction, which will consequently contribute to minimizing the possibility for the players to develop negative attitude toward the guild they belong to.

Comparison of Soil Profile Development Index of the Residuum Soils Derived from Conglomerate (역암지대(礫岩地帶) 잔적토양(殘積土壤)의 토양단면발달지수(土壤斷面發達指數) 비교(比較))

  • Kim, Jung-Kon;Jung, Yeun-Tae;Son, Il-Soo;Yoon, El-Soo;Um, Ki-Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.19 no.2
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    • pp.99-105
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    • 1986
  • This study was carried out to compare the soil profile development indices of Sinjeong series according to the distribution areas in Yeongnam district. The results obtained were as follows; 1. The soils studied were belong to fine loamy family of hilly residual soils derived from conglomerate. These soils were well drained and dominantly had 30-60% slopes, and used for forest. 2. The surfaces were typified by brownish red (5YR) or brownish yellow (10YR) loam, subsoils were clay loam of reddish brown (5YR) or reddish yellow (7.5YR) and the substrata were in dark reddish brown (5YR) or pink (7.5YR) sandy loam. 3. The indices of horizon development of the soils studied were from 0.18 to 0.38 and the index in illuvial horizons with clay films were over 0.36, that were higher than the others. The profile development indices were similar to each other ranging from 30.18 to 35.93. 4. Positive correlations were observed among horizon development indices and normalized values of structure, texture and consistences, but the relationship between profile development indices and consistences were not the same. 5. The amount of clay minerals formed in the soil horizons per 100g of parent materials were 1.1-1.6g and the rates of the clay leached were around 4.4-5.9%. 6. The sphericity and roundness of the gravels contained in the soils studied were 0.741 and 0.715, respectively. These similarity of "well rounded" gravels certify that the parent rocks were formed under similar environments.

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