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Effects of Medium Compositions and Plant Growth Regulators on in vitro Organogenesis in Cultured Explants of Platycodon grandiflorum Species

  • Kwon, Soo Jeong;Roy, Swapan Kumar;Kim, Hye-Rim;Moon, Young-Ja;Yoon, Ki-Hong;Woo, Sun Hee;Boo, Hee Ock;Koo, Jin-Woog;Kim, Hag Hyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.3
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    • pp.259-274
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    • 2017
  • Platycodon grandiflorum (Bell flower) is an important plant that has traditionally been used as herbal medicine for the treatment of cough, phlegm, sore throats, lung abscesses, chest pains, dysuria, and dysentery. The present study was initiated to investigate the feasibility of inducing shoot and root organogenesis in cultured explants of P. grandiflorum in a range of culture media and through use of various plant growth regulators (PGRs). The plantlets (Stem containing one node) were isolated and cultured on different concentrations of Murashige and Skoog (MS) medium supplemented with PGRs. We found that proliferation and elongation of shoots and roots could be achieved on 1/4 MS for P. grandiflorum with wild and green petals and on 1/8 MS for P. grandiflorum with double petals. The highest levels of development and elongation of adventitious shoots and roots were observed when petal explants were cultured on 1/4 MS (pH 3.8) supplemented with 5% sucrose. Increasing the agar concentration reduced shoot growth and rooting potential; nevertheless, the highest number of shoots and roots was observed on 0.6% agar. In the case of growth regulators, 1/4 MS supplemented with $1mg\;L^{-1}$ 6-benzylaminopurine (BA) was found to be best for shooting, although higher concentrations of BA tended to reduce shoot and root elongation. The highest number of shoots was achieved on $0.5mg{\cdot}L^{-1}$ thidiazuron (TDZ) from double petal explants grown on 1/8 MS. However, root and shoot elongation were found to decrease when TDZ concentrations were increased. Low concentrations of kinetin, naphthalene acetic acid, indole acetic acid, and 3-indole butyric acid induced shoot and root proliferation and elongation. Taken together, our study showed that low concentrations of PGRs induced the greatest root formation and elongation, showing that the optimal concentration of PGRs for shoot proliferation was species-dependent.

According to the clinical training, the study of stresses and coping ways of stresses (임상실습 수행시 스트레스와 스트레스 대처방법에 관한 연구)

  • Nam, Yong-Ok;Jang, Jong-Hwa;Kim, Sun-Sook
    • Journal of Korean society of Dental Hygiene
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    • v.2 no.2
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    • pp.145-158
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    • 2002
  • This is a section study for research of main cause and coping ways caused by stresses during the clinical experiment. It was researched by 231 students of third grade have finished clinical experiment until September though questionnaire. The methods for this study used the a stress measuring instrument thai was used by Hoa-Yun Jun and coping ways of stresses devised by Bell. We computed the frequency and the mean value by the SPSS 10.0 statistical data and we got the following result that from the experiment with t-test and the ANOVA analysis. 1. The mean value of stresses indicated rather higher states in figures 2,855 and the figures that were classified by each areas, indicated highest figures are affected by circumstances, that is 3.065. 2. As higher as the degree of satisfactions in their major, students feel stress less so, the result was not quite different between each of them (p=0.007). 3. The copping ways of stresses indicated, similar differences by using of long-term copping ways and short-term copping ways to cope with stresses(p=0.000). 4. In case of the students who failed college entrance exams and prepared for the next chance for more than one year, indicated highest figures that is 2.57 in short-term ways to cope with stresses, so it showed slightly differences between them(p=0.014). 5. According to the satisfactions of clinical experiments, there were not any statistical differences in long-term coping ways but, as smaller as the satisfactions, in that evidence of the students use short-term copping ways, it showed slightly differences between them(p=0.041). 6. According to the degree of stresses, low-stress groups use short-term coping ways more than high-stress groups, so it showed slightly differences between them(p=0.001). 7. As far as the using of long-term coping ways, the figures of between the lower stress groups and the higher stress groups indicated 2.89 and 2.97 respectably so, the figures were not so closed.

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Analysis on Multi-Components of Neurotransmitter Release in Response to Light of Retinal ON-Type Bipolar Cells (망막 ON형 쌍극세포의 광응답에 따른 다중성분의 전달물질 방출에 관한 해석)

  • Jung, Nam-Chae
    • Journal of the Institute of Convergence Signal Processing
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    • v.14 no.4
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    • pp.222-230
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    • 2013
  • Retinal bipolar cells according to the light stimulus respond to potential slowly, emit neurotransmitter release(glutamine acid) to depend on membrane potential. In this paper, the several physiological information on neurotransmitter release mechanism in the presynaptic terminal of the ON-type bipolar cells are incorporated into the formula model. The source of fast components and slow components of neurotransmitter release was arranged in parallel, this model was able to reproduce the membrane potential and intracellular $Ca^{2+}$ concentration dependence of neurotransmitter release faithfully. In addition, because the fast releasable components of neurotransmitter was represented by the membrane potential dependence of trapezoid type, whereas the slow releasable components was represented by the membrane potential dependence of a bell type, $Ca^{2+}$ concentration rise in intracellular is suppressed by $Ca^{2+}$ buffer to reduce slow releasable components, it was confirmed that the membrane potential dependence of neurotransmitter release was characteristics of a trapezoid type. And, in the light response of ON type bipolar cell, the result of the simulation of the neurotransmitter release caused by the components of transient and persistent was that the start of light response occurred the fast release of neurotransmitter, it was confirmed that the transient component and persistent component of the light response occurred the slow release. It was confirmed that the later of persistent component of the light response occurred due to the continuous release by synaptic vesicle supplemented from the storage pool.

xDSL 표준화동향

  • 김기호
    • Information and Communications Magazine
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    • v.14 no.12
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    • pp.77-85
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    • 1997
  • 지금까지 access network에서의 xDSL의 위치를 살펴보고 DSL, HDSL, ADSL 및 VDSL을 중심으로 기술 및 최근의 표준화동향을 살펴보았다. ITU의 조사에 따르면 97년 기준으로 전세계 약 8억가구에 copper access line이 연결되어 있고 그중의 70%이상이 가정에 연결되어 있는데 증가추세도 북미의 경우 년3.5%, 서유럽과 태평양연안의 경우 년4.5%의 성장율을 보이고 있어 2000년경에는 10억가구에 다다를 전망이다. 미국은 97년기준 약 1억가구에 1억6000만 copper access line이 연결되어 있는데 Yankee Group이 조사한 바에 따르면 2000년을 기준으로 3백만가구에 ADSL/RADSL/SDSL이 연결되고 관련 revenue는 10억달러에 이를것으로 추산하고 있다. 전세계 xDSL 시장규모를 미국의 5배정도로 보면 2000년경에는 전세계 twist pair의 2%정도에 xDSL이 연결되어 ADSL/RADSL/SDSL 관련 세계시장규모가 50억달러에 이르고 시장성장률은 30%에 이를 것으로 보고 있다. 현재 이러한 xDSL의 표준화작업은 3장에서 언급한 바와 같이 ANSI T1, ETSI에서 주로 주도되고 있으나 ITU-T, IEEE, ADSL Forum, ATM Forum, DAVIC 등 다른 group과 활발한 liasion meeting 을 통해서도 협력안을 마련하려 노력하고 있다. 예를 들어 DAVIC은 long range(1.5Km이상) PMD규격으로 ADSL을, mid range($300m\sim1.5km$) PMD규격으로 VDSL을, short range(300m이하) PMD규격으로 CAP방식의 FTTC를 각각구정하여 xDSL을 수용하고 있다. Microsoft의 CEO인 Bill Gates가 'Bandwidth bottleneck. No question. that's the biggest obstacle.'이라고 천명한 바와 같이 정보사회로의 진전에 있어 bandwidth는 시급히 해결되어야 할 문제이다. 정보사회의 꿈을 실현하고자 1993년부터 시작된 In-formation Infrastructure의 구축노력이 그동안 다양하게 시도되어왔으나 숱한 우여곡절과 실패를 겪은 telco등의 서비스업자들이 이제는 너무 큰 꿈을 쫓기보다는 internet이라는 현실적인 시장에서 xDSL과 cablemodem을 바탕으로 차근차근 새로 시작하고 있다고 생각된다. 그동안 ADSL, Forum을 중심으로한 일부 technology evangelist들은 전세계 8억가구에 깔려있는 copper twist pair를 경제적인 'the last mile solution'으로 활용할 것을 줄기차게 주장해왔다. 그동안 xDSL은 VDSL이라는 차세대 대안을 제시하여 interim solution이라는 울타리를 벗어나는데 성공하였을 뿐만 아니라 IDSL, SDSL, RADSL등의 새로운 고객지향적 xDSL을 개발하여 선택의 폭을 넓혀주었다. xDSL vendor들간의 치열한 경쟁속에서 값싼 xDSL 시스템들이 속속 등장하고 있으며, DSLAM[8]과 같은 switch solution도 제시되어 기존의 copper twist pair를 이용해 값싸게 다양한서비스를 제공해 줄 수 있는 기틀을 마련한 것이 오늘날의 xDSL의 성공요인이라고 생각된다. 일찌기 Bell Atlantic의 CEO Ray Smith가 'Yes. ADSL is an interim solution that will be with us for forty years.'라고 간파한대로 xDSL은 fiber가 좀 더 값싼 해결책이 될 때까지 당분간 access network에서 확고한 자리를 구축할 것으로 보인다. 최근들어 우리나라의 초고속정보통신망도 ADSL 과 VDSL을 바탕으로 FTTH으로 진화하는 전략을 수립한것은 우리의 현실과 세계적인 추세를 반영한 일이라 여겨진다.

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An Experimental Study on the Heat Transfer Characteristics during Outward Melting Process of Ice in a Vertical Cylinder(comparison of thermal performance on the flow direction of working fluid) (수직원통형 빙축열조내 얼음의 외향용융과정시 전열특성에 관한 실험적 연구(작동 유체의 유입 방향에 따른 비교))

  • Kim, D.H.;Kim, D.C.;Kim, I.K.;Kim, Y.K.;Yim, C.S.
    • Solar Energy
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    • v.16 no.2
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    • pp.113-122
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    • 1996
  • This study presents experimental results of heat transfer characteristics of P.C.M. during outward melting process in a vertical cylinder. The experiment was carried out in six conditions, i. e., three different inlet temperature($7^{\circ}C,\;4^{\circ}C\;and\;1^{\circ}C$) and two directions of working fluid(upward and downward). Melting P.C.M. produced a bell-shaped phase change interface. When the inlet temperature was $7^{\circ}C$, the lower region remained at $4^{\circ}C$ until the temperature of upper region reached $4^{\circ}C$. This was due to the state of maximum density of the lower region. When the direction of the working fluid in the case of $7^{\circ}C$, inlet temperature, was upward, the rate of melting and the total melting energy were higher than when it's direction was downward. But the rate of melting and the total melting energy appeared higher value as it's direction was downward when the inlet temperature is $4^{\circ}C$ and $1^{\circ}C$.

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Alternaria mycotoxins and its incidence in fruits and vegetables

  • Patriarca, Andrea
    • 한국균학회소식:학술대회논문집
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    • 2018.05a
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    • pp.13-13
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    • 2018
  • Alternaria is a ubiquitous fungal genus, widely distributed in the environment and a range of different habitats. It includes both plant pathogenic and saprophytic species, which can affect crops in the field or cause post-harvest spoilage of plant fruits and kernels. Numerous Alternaria species cause damage to agricultural products including cereal grains, fruits and vegetables, and are responsible for severe economic losses worldwide. Most Alternaria species have the ability to produce a variety of secondary metabolites, which may play important roles in plant pathology as well as food quality and safety. Alternariol (AOH), alternariol monomethyl ether (AME), tenuazonic acid (TeA), tentoxin (TEN) and altenuene (ALT) are considered the main Alternaria compounds thought to pose a risk to human health. However, food-borne Alternaria species are able to produce many additional metabolites, whose toxicity has been tested incompletely or not tested at all. Both alternariols are mutagenic and their presence in cereal grain has been associated with high levels of human esophageal cancer in China. TeA exerts cytotoxic and phytotoxic properties, and is acutely toxic in different animal species, causing hemorrhages in several organs. The possible involvement of TA in the etiology of onyalai, a human hematological disorder occurring in Africa, has been suggested. Altertoxins (ALXs) have been found to be more potent mutagens and acutely toxic to mice than AOH and AME. Other metabolites, such as TEN, are reported to be phytotoxins, and their toxicity on animals has not been demonstrated up to now. Vegetable foods infected by Alternaria rot are obviously not suitable for consumption. Thus, whole fresh fruits are not believed to contribute significantly with Alternaria toxins to human exposure. However, processed vegetable products may introduce considerable amounts of these toxins to the human diet if decayed or moldy fruit is not removed before processing. The taxonomy of the genus is not well defined yet, which makes it difficult to establish an accurate relationship between the contaminant species and their associated mycotoxins. Great efforts have been made to organize taxa into subgeneric taxonomic levels, especially for the small-spored, food associated species, which are closely related and constitute the most relevant food pathogens from this genus. Several crops of agricultural value are susceptible to infection by different Alternaria species and can contribute to the entry of Alternaria mycotoxins in the food chain. The distribution of Alternaria species was studied in different commodities grown in Argentina. These food populations were characterized through a polyphasic approach, with special interest in their secondary metabolite profiles, to understand their full chemical potential. Alternaria species associated with tomato, bell pepper, blueberry, apples and wheat cultivated in Argentina showed a surprisingly high metabolomic and mycotoxigenic potential. The natural occurrence of Alternaria toxins in these foods was also investigated. The results here presented will provide background for discussion on regulations for Alternaria toxins in foods.

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Distribution of Heterotrophic Bacteria and Extracellular Enzyme Activities of Bacteria in the Sediment of South Sea, Korea (남해 퇴적토에서 종속영양 세균의 분포 및 세포의 효소 활성력)

  • 김상진;이건형
    • Korean Journal of Microbiology
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    • v.30 no.5
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    • pp.383-390
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    • 1992
  • In the periods of July 31 to August 10. 1988 and March 9 to 13. 1989. sediment samples were collected from the South Sea stations (010] to 092]) located in the area from $N 32^{\circ}$/30' to $34^{\circ}$/30', of latitude and from E $123^{\circ}$ 30' to $128^{\circ}$30' of longitude. These samples were analyzed for the number of total heterotrophic bacteria and extracellular digesting enzyme activities. In the 1989 spring period the number of heterotrophic bacteria in the sediment surface layer was increased more than 100 times at the maximum compared to that in the 1988 summer period. The proportion of fresh water bacteria to total heterotrophic bacteria was also higher in the spring period than the summer period. The extracellular digesting enzyme activities were higher in spring season than summer. Although the water content of sediment in the spring period was lower than that the summer period. the ash weight indicating organic material content was higher. These results means that the diameters of sediment particles were larger in spring than summer but the input of organic material into the sediment was greater. Based on these results bacterial distributions in the sediment layer of South Sea depend greatly on the season due to the effect of fresh water. During the spring season plankton could grow extensively owing to the inorganic nutrients input by the vertical mixing in the water column, then be precipitated into the sediment. Organic nutrients supplied from enzymatic degradation of polymeric particle from plankton can increase the bacterial number, too.

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Prostaglandin $E_1$ Increases cGMP Levels in Beating Rabbit Atria: Lack of Effects of $PGE_1$-induced Cyclic Nucleotides on Secretory and Contractile Functions

  • Jin, Xuan Shun;Quan, He Xiu;Kim, Sun-Young;Park, Sung-Hun;Kim, Sung-Zoo;Lee, Ho-Sub;Cho, Kyung-Woo
    • The Korean Journal of Physiology and Pharmacology
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    • v.11 no.5
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    • pp.175-182
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    • 2007
  • Members of prostaglandin(PG) E-series elicit cellular effects mainly through adenylyl cyclase-cAMP signaling. The role of $PGE_2$-induced increase in cAMP has been shown to be compartmentalized in the cardiac myocytes: $PGE_2$-induced increase of cAMP is not involved in the control of cardiomyocytic contraction. The purpose of the present study was to define the effect of $PGE_1$ on the cGMP levels and the role of $PGE_1$ in the atrial secretory function. Experiments were performed in perfused beating rabbit atria and atrial contractile responses, cGMP and cAMP efflux, and atrial natriuretic peptide(ANP) secretion were measured. $PGE_1$ increased cGMP as well as cAMP efflux concentration in a concentration-dependent manner, however, no significant changes in atrial secretory responses were observed(with $1.0{\mu}M\;PGE_1$; for cGMP, $144.76{\pm}37.5%$, n=11 versus $-16.81{\pm}4.76%$, n=6, control, p<0.01; for cAMP, $187.60{\pm}41.52%$, n=11 versus $7.38{\pm}19.44%$, n=6, control, p<0.01). $PGE_1$ decreased atrial dynamics slightly but transiently, whereas $PGE_2$ showed similar effects but with lower potency. Isoproterenol increased atrial cAMP efflux(with 2.0 nM; $145.71{\pm}41.89$, n=5 versus $7.38{\pm}19.44%$, n=6, control, p<0.05) and mechanical dynamics and decreased ANP secretion. The $PGE_1$-induced increase in cGMP efflux showed a bell-shaped concentration-response curve. $PGE_1$-induced increase of cGMP efflux was not observed in the presence of L-NAME, an inhibitor of nitric oxide(NO) synthase, or ODQ, an inhibitor of NO-sensitive guanylyl cyclase. L-NAME and ODQ showed no significant effect on the $PGE_1$-induced transient decrease of atrial dynamics. These data indicate that $PGE_1$ increases cGMP levels via NO-soluble GC signaling in the cardiac atrium and also show that $PGE_1$-induced increases in cGMP and cAMP levels are not involved in the regulation of atrial secretory and contractile functions.

A taxonomic study of the genus Hosta in Korea (한국산 비비추속(Hosta Tratt.) 식물의 분류학적 연구)

  • Jo, Hyun;Kim, Muyeol
    • Korean Journal of Plant Taxonomy
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    • v.47 no.1
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    • pp.27-45
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    • 2017
  • A taxonomic study of the genus Hosta (Lilliaceae) in Korea was conducted based on morphological characteristics. Hosta clausa varieties share several characteristics (rhizomes with underground stolons, smooth scapes, and purple anthers). Hosta clausa var. normalis has an open perianth, long pistils, and fertile fruit. H. clausa var. clausa can be distinguished from H. clausa var. normalis by its closed perianth and sterile fruit. H. clausa var. geumgangensis has sterile fruit, green petioles, short pistils, and short narrow perianth tubes. H. clausa var. ensata has very narrow, sword-shaped leaves. It can be included in H. clausa var. normalis, which has a large degree of variation in leaf and petiole shape. H. jonesii has smooth scapes, yellow anthers, and bell-shaped flowers. It can be distinguished from the related species H. tsushimensis found on the Tushima islands in Japan by its wide perianth tube. H. yingeri is distinguished by its funnel-shaped flowers, 3 long and 3 short stamens, and smooth scape; the related species H. laevigata has 6 equally-sized stamens, narrowly lanceolate leaves, and wavy leaf margins. Because H. laevigata is not found in its type locality Isl. Heugsando, its taxonomic position must be determined at a later time. H. minor shares several characteristics (scapes with ridges and yellow-colored anthers) with the related taxa H. venusta. However, H. venusta is changed to H. minor var. venusta because it has overlapping characteristics in plant size, number of flowers, leaf size, and leaf shape. Consequently, the genus Hosta in Korea is classified into 5 species, 3 varieties, and 6 forms.

Vehicle Detection using Feature Points with Directional Features (방향성 특징을 가지는 특징 점에 의한 차량 검출)

  • Choi Dong-Hyuk;Kim Byoung-Soo
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.42 no.2 s.302
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    • pp.11-18
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    • 2005
  • To detect vehicles in image, first the image is transformed with the steerable pyramid which has independent directions and levels. Feature vectors are the collection of filter responses at different scales of a steerable image pyramid. For the detection of vehicles in image, feature vectors in feature points of the vehicle image is used. First the feature points are selected with the grid points in vehicle image that are evenly spaced, and second, the feature points are comer points which m selected by human, and last the feature points are corner Points which are selected in grid points. Next the feature vectors of the model vehicle image we compared the patch of the test images, and if the distance of the model and the patch of the test images is lower than the predefined threshold, the input patch is decided to a vehicle. In experiment, the total 11,191 vehicle images are captured at day(10,576) and night(624) in the two local roads. And the $92.0\%$ at day and $87.3\%$ at night detection rate is achieved.