• 대한약침학회지
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• 제10권2호통권23호
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• pp.81-86
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• 2007

Objectives : This study was conducted to carry out quantitative evaluation and safety of Sweet Bee Venom. Methods : Content analysis was done using HPLC, measurement of LD$^{50}$ was conducted intravenous, subcutaneous, and intramuscular injection to the ICR mice. Results : 1. According to HPLC analysis, removal of the enzymes containing phospholipase A2 was successfully rendered on Sweet Bee Venom. And analyzing melittin content, Sweet Bee Venom contained 12% more melittin than Bee Venom. 2. LD$^{50}$ of ICR mice with Sweet Bee Venom was more than 20mg/kg in subcutaneous injection and intravenous injection, between 15mg/kg and 20mg/kg in muscular injection. 3. LD$^{50}$ of ICR mice with Bee Venom was between 6 and 9mg/kg in subcutaneous injection and intravenous injection, and more than 9mg/kg in muscular injection. Conclusion : Above results indicate that Sweet Bee Venom was more safe than Bee Venom and the process of removing enzymes was well rendered in Sweet Bee Venom.

• 한국유체기계학회 논문집
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• 제19권3호
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• pp.37-42
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• 2016

The demand for wireless technology in plant structure has recently been increasing due to several advantages such as installation cost reduction, easy placement, easy extension and aesthetic benefits. Among the many wireless technologies, ZigBee is one of the most useful for plant structure; a wireless plant networking system can be configured using ZigBee alone. This research proposes a ZigBee to use for extreme cold region and thereby enable integration of wired and wireless plant monitoring systems. In this study, in order to assess the performance of ZigBee measured data by thermocouple were examined based on the results from laboratory tests between existing ZigBee and developed ZigBee. From the experiment results, performance of developed ZigBee in harsh environment can be increased well.

• 대한한방내과학회지
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• 제32권2호
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• pp.203-216
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• 2011

Objectives : This study was conducted to evaluate the protective effects of bee venom on lipopolysaccharide (LPS)-induced chronic obstructive pulmonary disease (COPD). Methods : In this study, LPS was administrated to Balb/c mice to induce a disease that resembles COPD. 2 hr prior to LPS administration, mice were treated with bee venom via an intraperitoneal injection. Total cell number and neutrophils number in bronchoalveolar lavage fluid were counted and pro-inflammatory cytokines were also measured. For histologic analysis, periodic acid Schiff (PAS) and hematoxylin and eosin (H&E) stains were evaluated. Proliferating cell nuclear antigens (PCNA) were also assessed by immunohistochemistry. Results : On 7 days after LPS stimulation, influx of neutrophils significantly decreased in the bee venom group, compared with the COPD group. In addition, TNF-a and IL-6 levels decreased in bee venom group. Histological results also demonstrated the attenuation effect of bee venom on LPS-induced lung inflammation. Conclusions : These data suggest that bee venom has protective effects on LPS-induced lung inflammation. Therefore, bee venom may represent a novel therapeutic agent for lung inflammation and in particular for COPD.

• 대한약침학회지
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• 제6권2호
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• pp.105-111
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• 2003

Objective : The purpose of this study was to investigate the stability of bee venom according to the keeping method and period. Method : The author observed microbial contamination of bee venom in nutrient agar, broth, YPD agar and YPD media and antibacterial activity for S. aureus, E. coli manufactured 12, 6 and 3 months ago as the two type of room temperature and $4^{\circ}C$ cold storage. Results : 1. 1:3,000 and 1:4,000 diluted bee venom solution did not show microbial contamination both room temperature and cold storage within twelve months. 2. There was antibacterial activity of diluted bee venom for S. aureus in cold storage within twelve months and there was no antibacterial activity of diluted bee venom for S. aureus in twelve months, room temperature storage. 3. We could not observe the zone of inhibition around paper disc of all for E.coli. in 1:3,000, 1:30,000 and 1:3,000,000 diluted bee venom solution, respectively. According to results, we expect that diluted bee venom solution is stable both cold and room temperature storage within twelve months.

• Journal of Acupuncture Research
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• 제15권2호
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• pp.97-103
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• 1998

Purpose: In order to study the anti-inflammatory and analgesic effects of Bee Venom Therapy, writhing syndrome, carrageenin induced edema, and complete adjuvant induced arthritis were experimented. Methods: 1. We used rats of writhing syndrom induced by acetic acid injection. Group I was treated with Acupuncture therapy. Group II was treated with Bee Venom thearpy. we compared with Group I, II and the control group. 2. We used rats of paw edema induced by carrageenin injection. Group I Was treated with Acupuncture therapy, Group II was treated with Bee Venom thearpy we compared with Group I, II and the control group. 3. We used rats of arthritis induced by Freud's complete adjuvant injection. Group I was treated with Acupuncture therapy, Group II was treated with Bee Venom thearpy we compared with GroupI,II and the control group. Results: 1. Analgesic effects on writhing syndrome induced by acetic acid injection showed statistical significance in Bee Venom treated group as compared with the control group. 2. The paw edema induced by carrageenin injection was decreased with statistical significance in Bee Venom treated group as compared with the control group. 3. The arthritis induced by Freud's complete adjuvant injection wasn't significant. Conclusion: Bee Venom Therapy was effective on inflammatory disease.

• Journal of Acupuncture Research
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• 제20권3호
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• pp.154-165
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• 2003

Objective : to research the trend of the study related to Bee venom and o establish the hereafter direction for the study on Bee Venom therapy. Methods : We reviewed the domestic papers published last ten years(1992-2001). Results: 1.We have searched 53 papers in 7 journals and the pattern of study was as follow: the experimental studies were 33, the clinical studies were 10 and the reviewed studies were 10. 2. The experimental studies were 2 papers of analysis of Bee Venom, 3 papers of safety assessment, 1 paper on production of antibody against Bee Venom and 26 paper of safety assessment. 3. Bee Venom used in studies was made in Korea, China and U.S.A.. There were differences of component and effect according to the place of production. 4. There were the experimental studies of LD50 in mouse, acute toxicity, local irritation test, antigenicity and pyrogen test of Bee Venom. Conclusions : We need more studies of unification of term about Bee Venom, difference according to the place of production, clinical safety and effects.

• Journal of Acupuncture Research
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• 제30권1호
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• pp.57-70
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• 2013

This study was to investigated the effects of the bee venom on inhibition of cell growth via upregulation of death receptor expression in the A549 human lung cancer cells. Bee venom(1-5 ${\mu}g$/ml) inhibited the growth of A549 lung cancer cells by the induction of apoptotic cell death in a dose dependent manner. Consistent with apoptotic cell death, expression of TNFR1, Fas, death receptors(DR) 3, 4 and 6 was increased in the cells. Expression of DR downstream pro-apoptotic proteins including caspase-3, -9 and Bax was concomitantly increased, but the expression of Bcl-2, NF-${\kappa}B$ were inhibited by treatment with bee venom in A549 cells. Moreover, deletion of DR3, DR4 by small interfering RNA significantly reversed bee venom-induced cell growth inhibitory effect, whereas Apo3L strengthened anti-proliferative effect of bee venom through enhancement of DR3 expression. These results suggest that bee venom should exert anti-tumor effect through induction of apoptotic cell death in lung cancer cells via enhancement of death receptor expression, and that bee venom could be a promising agent for preventing and treating lung cancer.

• 한국정보통신학회논문지
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• 제11권2호
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• pp.416-421
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• 2007

ZigBee는 저전력 특성을 기반으로 무선 센서 네트워크의 전송 프로토콜로 활용되고 있다. ZigBee 네트워크에서 연속적인 데이터 전송률이 ZigBee에서 처리 가능한 최대 전송률 수준으로 요구되는 경우에 데이터를 중계하는 라우터가 수신과 송신을 동시에 처리 할 수 없기 때문에 실질 전송률은 절반 이하로 떨어지게 된다. 본 논문에서는 2개의 송수신 모듈을 장착하여 수신과 송신을 동시에 처리할 수 있는 라우터를 설계하고 구현하였다. 또한, 송수신을 동시에 처리할 수 있도록 서로 다른 채널을 할당하는 중앙집중식 채널할당 알고리즘을 제시하였다. 실험을 통해 제안한 이중 채널 라우터의 최대 전송률이 일반 라우터의 2배에 가까운 150kbps에 이르는 것을 보였다.

• 한방안이비인후피부과학회지
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• 제23권2호
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• pp.27-40
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• 2010

Background and Objective : Increasing interest in anti-aging and anti-wrinkling agents for the skin has triggered the recent outflow of researches and studies in this field. This study was designed to investigate the effects of bee venom on skin wrinkling and skin aging by testing the skin wrinkling, skin elasticity, trans-epidermal water loss (TEWL), free radical level, anti-oxidative agent level, and skin tissue after infusion of bee venom on hairless mouse. Materials and Methods : Fifteen hairless mice aged between 36~40 weeks were divided randomly into 3 Group; the Bee Venom Syringe Group, the Bee Venom Needle Group, and the control group. The Bee Venom Syringe Group were injected subcutaneously with bee venom (0.1cc in total) using an insulin syringe on three spots in the lumbar spine (one spot on the center and two spots 1~2cm to the side bilaterally). The Bee Venom Needle Group were pricked with bee venom-smeared acupuncture needles on three longitudinal spots in the lumbar spine each 1cm apart, after which the needles were removed 10 minutes later. The Control Group did not receive any form of intervention. All procedures took place thrice a week for four weeks, during which the mice were allowed free access to water and fodder. The mice were measured and compared in the weight, skin wrinkling scale, skin elasticity, and TEWL before and after the experiment. After the experiment, blood samples were taken to measure the free radical and anti-oxidative agent level, and the skin tissue was sliced for examination. Data was analyzed using the SPSS program (ver 12.0). The ANOVA analysis was used to compare and contrast the three groups, and t-test for paired samples was used to evaluate skin-wrinkling before and after experiment. The cut-off p-value of significance was set at p<0.05. Results : 1. Administration of bee venom did not cause serious weight loss or gain. 2. Compared to the control group, the Bee Venom Syringe Group and the Bee Venom Needle Group both showed a decrease in skin wrinkling scale after intervention. Especially, the Bee Venom Syringe Group showed a significant decrease (p<0.05). 3. Compared to the control group, the Bee Venom Syringe Group and the Bee Venom Needle Group both showed an increase in skin elasticity. Especially, the Bee Venom Syringe Group showed a significant increase (p<0.05). 4. No significant change in TEWL was found in the mice in all the three groups before and after experiment. 5. Free radical level was normal in all 15 mice in all the three groups, and anti-oxidative agent was not significantly different across the three groups. 6. The Bee Venom Syringe Group, the Bee Venom Needle Group, and the control group did not show any significant difference in the thickness of epidermis and dermis, infiltration of inflammatory cells, and skin wrinkling. The epidermis layer was relatively better preserved in the Bee Venom Syringe Group as compared to the Bee Venom Needle Group and the control group. Conclusion : Direct injection of bee venom on the hairless mouse using a syringe was found to improve wrinkling of the skin and increase skin elasticity but did not show effectiveness on skin dryness due to water loss. The bee venom appears to have suppressive effects on skin wrinkling, one of the symptoms of skin aging, through a process independent of suppression of free radicals or increase of anti-oxidative agent.

• 대한전자공학회논문지TC
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• 제43권1호
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• pp.79-88
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• 2006

본 논문에서는 RFID와 근거리 무선 통신 기술인 ZigBee를 접목시켜 유비쿼터스 u-Health 시스템을 구현하였다. RFID 태그인식 기술, ZigBee의 데이터 송수신 기술을 이용하는 무선 프로토콜 키트를 만들었고 TinyOS에서 ZigBee의 소프트웨어를 구현하였다. RFID와 접목 가능한 무선 통신 기술들은 Bluetooth, ZigBee, 802.11x WLAN 등이 있다. 논문에서 제시한 환경은 무인간병과 같은 u-Health 시스템으로서, 조밀한 sensor 네트워크 환경을 갖게 되는 병원 등의 환경에 적합하도록 ZigBee를 사용한 것이다. RFID와 ZigBee가 부착되는 디바이스들은 앞으로 팔찌, 손목시계, 반지 등의 크기로 소형화 될 것이다. 본 논문에서 제안하는 RFID와 ZigBee 통합 환경은 WBAN(Wireless Body Area Network) 및 WPAN(Wireless Person Area Network)환경에서 상태 정보를 인식하여 원하는 action들로 반응하도록 요구되는 응용에 활용될 수 있다. 태그의 정보와 환자의 상태에 따라 여러 가지 제약사항을 두어 환자의 단말기에 문자정보의 형태로 LCD에 표시하거나, 음성정보로 메시지를 전송하도록 설계하고 구축하였다 RFID는 어플리케이션의 목적에 맞춰 다양한 무선통신 기술과의 결합을 이루어 나갈 것이다.