• Environmental Engineering Research
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• 제14권1호
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• pp.63-67
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• 2009

Escherichia coli K-12 (E. coli K-12) is a representative indicator globally used for distinguishing and monitoring dynamic fates of pathogenic microorganisms in the environment. This study investigated how to most critically quantify lacZ ($\beta$-galactosidase) gene in E. coli K-12 by two different real-time polymerase chain reaction (real-time PCR) in association with three different DNA extraction practices. Three DNA extractions, i.e., sodium dodecyl sulfate (SDS)/proteinase K, magnetic beads and guanidium thiocyanate (GTC)/silica matrix were each compared for extracting total genomic DNA from E. coli K-12. Among them, GTC/silica matrix and magnetic beads beating similarly worked out to have the highest (22-23 ng/${\mu}L$) concentration of DNA extracted, but employing SDS/proteinase K had the lowest (10 ng/${\mu}L$) concentration of DNA retrieved. There were no significant differences in the quantification of the copy numbers of lacZ gene between SYBR Green I qPCR and QProbe-qPCR. However, SYBR Green I qPCR obtained somewhat higher copy number as $1{\times}10^8$ copies. It was decided that GTC/silica matrix extraction or magnetic beads beating in combination with SYBR Green I qPCR can be preferably applied for more effectively quantifying specific gene from a pure culture of microorganism.

• 한국멀티미디어학회논문지
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• 제14권11호
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• pp.1507-1515
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• 2011

편종의 소리합성을 위한 연구의 첫 단계로 FFT 분석을 통하여 그 음색을 분석하였다. 현대 편종 음계의 음고는 서양의 12평균율의 음고와 큰 차이가 없었다. 편종의 음색은 시간의 흐름에 따라 변화한다. 타격시 모든 주파수 대역에 걸쳐 생성된 노이즈들은 단시간에 사라지고, 고유 배음만이 남는다. 그 후 높은 주파수 성분의 배음부터 차례로 소멸되고 여음부에는 두개의 배음만이 남는데, 이 때 남은 배음의 주파수에 의하여 맥놀이 현상인 발견된다. 본 연구에서 사용된 편종은 비교적 최근에 제작된 편종으로 추후 고대 편종의 음색에 관한 연구가 병행된다면 편종 원형의 소리 합성이 가능할 것이다.

• Tribology and Lubricants
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• 제14권1호
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• pp.7-13
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• 1998

The static characteristics of a five-pad tilting pad journal bearing of load on pad (LOP) type have been investigated experimentally under the different values of bearing load and shaft speed. The diameter and length of the bearing are 300.91 mrn and 149.8 mm, reslx;ctively. Circumferential distribution of the film pressure, film thickness, journal surface temperature and beating surface temperature are measured. A noticeable inlet pressure rise is observed at the entrance of each pad, especially the bottom pad. The inlet pressure is increased by the increase of shaft speed as well as bearing load. In the five-pad tilting pad joumal bearing of LOP type, almost all of beating load is being carried only by the bottom pad. The maximum bearing surface temperature is observed at near the minimum film thickness. It is observed that the metal temperature of the mid-plane is higher than that of the edge at the inlet region, while the metal temperature of the edge is higher than that of the mid-plane at the outlet region.

• 지역사회간호학회지
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• 제12권2호
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• pp.482-490
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• 2001

Purpose I investigated how beliefs in punishment have influence on child abuse. Also, I investigated how socio-demographic variables of parents' have influences on child abuse by parents. Sampling was conveniently and total 160 parents replied to the study. Data analysis was done by descriptive statistics, Pearson's correlation using SPSSWIN. The results were as follows. Mild child abuse: 'Throwing the object at me(21.1%)', 'Clutching, and pushing (21.0%)', 'Slapping on the cheek (19.1 %), Severe child abuse: 'Kicking, pounding and biting(4.1%)', 'Rod, stick, belt, broom beating using a various objects such as(51.7%)', 'Beating all over the body(6.8%)'. Very severe child abuse: 'Burning with cigarette (0%)', 'Threatening me with a knife, a hammer, a naxe, a gimlet the objects such as(0.7%)', 'Injuring me with the objects such as (0%)', 'Hospitalized by belting(0%)'. Correlations of major variables were as follows. Physical child abuse by elementary student's parents has significantly positive correlation with beliefs in punishment(r= .244 p= .003) and has significantly negative correlation with parent's age(r=-.273, p=.001). But physical child abuse has not significant correlations with family income, education. Conclusion: Beliefs in punishment of parents have influences on child abuse. To prevent child abuse, effective parenting program development is required.

• 연구논문집
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• 통권29호
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• pp.111-121
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• 1999

고속 Rapier loom에서 경사와 위사의 조직을 완성하여 직물을 제직하는 위입과 바듸침 기구는 캠구동 방식으로 동일축선 상에 4개의 캠이 설치되어 있다. 이들 캠들은 2개씩 한쌍을 이루면서 서로 공액운동을 수행하고 있으므로 정확한 설계에 의한 동기화운동이 필수적이다. 따라서 본 연구는 전체 거동의 파악을 위한 시스템의 정확한 해석과 컴퓨터 시뮬레이션을 통한 Rapier loom의 고속화를 목적으로, 위입과 바듸침기구의 곡선선정 및 캠의 회전에 따라 정확한 운동을 유도할 수 있도록 2가지 운동의 연관성은 물론 동시에 화면상에 구현할 수 있는 4개의 캠기구에 대한 시뮬레이션 프로그램을 개발하는 것이다.

• Journal of Advanced Marine Engineering and Technology
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• 제28권2호
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• pp.382-392
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• 2004

The usage of constant velocity (CV) joint is effective for motorboats on gliding regime of the motion. During transition on the gliding when angle of the CV differs from null on driving and driven composite shafts there are moments of the second order. Excitation of oscillations of the second order moments occurs when driving shafts transmits a variable torque. which generates through CV joint a lateral moment acting on the bearing. As a result of oscillations from a resonating harmonic of a shafting the harmonic with the greater or periodically varying amplitude for power condition trough transferring to nominal power 144kW. Beating conditions coincide with third mode having frequency 45.486 Hz. In that case there is high increasing of the equivalent stresses. The forming of the stiffness of the composite material is concerned to use most orientation of the layer angle in the range of $\pm$60 degrees relatively of shaft axis. Application of that angles for layer orientation gives possibility to avoid high disturbance of the shafting for motorboat transition regime.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2012년도 추계학술대회
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• pp.1009-1011
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• 2012

본 논문에서는 두개의 Unpolarized F-P LD를 이용한 상호주입 잠김 기반의 파장 가변 광원을 구현하였고, 온도 변화에 따른 특성을 분석하였다. 온도 변화에 따라 파장의 가변 범위가 최대 약 2nm이고, 상대적 밀도 잡음(RIN)는 최저 -110dB/Hz임을 확인하였다. 또한 온도가 높을수록 상대적 밀도 잡음(RIN)는 높아지고, Eye Pattern에 Beating Noise가 발생하는 것을 알 수 있었다.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 1999년도 Proceedings of Pre-symposium of the 10th ISWPC
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• pp.172-177
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• 1999

Effect of fiber treatment with cellulase (Liftase A40), and with two refining methods (Valley beating and impact refining) on wood fiber and handsheet properties were investigated at three refining levels (300, 400, and 500ml) for two furnishes (NBKP and LBKP). Part of the treated furnishes were classified by 150 mesh screen into fine-free fiber, and fines. Fiber length analysis, WRV, zero-span strength, and other handsheet mechanical properties were compared. The study showed that Liftase A40 lowered the zero-span and the folding endurance of both furnishes (NBKP much more and LBKP much less). Pretreatment with Liftase A 40 followed by refining significantly lowered the fiber length and refining energy to reach to the target freeness. Impact refining, which is done by hitting the fibers vertically with rod at 20% solid content, kept the fiber length increased WRV, and improved handsheet mechanical properties much more than valley beating. Properties of fines from different sources were compared in detail in the study.

• 펄프종이기술
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• 제35권2호
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• pp.58-64
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• 2003

Because of its tendency of making strong Hoc on the fiber surface with fines, Meogujaengi has not been valued as a material of Hanji. As an attempt to manufacture high value-added products using the material made from Jumchihanji, this study performed morphological classification and chemical component analysis and selection of pulping of Meogujaengi method. As a result, it can be concluded as follows, 1. Meogujaengi is assumed to be a local variety of Broussonetia karinoki and its outward appearance is distinguished from Broussonetia kazinoki. 2. The bast fiber of Meogujaengi is longer and thinner than that of Broussonetia papyrifera or Broussonetia kazinoki. However, because of the coarse linear of fiber tissue, there are many clusters. 3. The cluster phenomenon of Meogujaengi is nothing to do with its chemical components. Although the contents of its chemical components are different from those of Broussonetia kazinoki, no component was found that obstructs pulping. 4. The pretreatment for suppressing the occurrence of clusters of Meogujaengi was effective, and it was necessary to do secondary beating using hollander beater after beating mixed with PAM using knife beater.

• 펄프종이기술
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• 제43권2호
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• pp.31-39
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• 2011

The applicability of arrowroots as materials for paper manufacture was investigated by evaluating the characteristics of arrowroots pulping and pulps, and the surface characteristics of paper made of arrowroots. The results is as follows. The yield of grounded pulp, alkaline pulp, grounded bleach pulp, and alkaline bleach pulp are 98.0%, 37.7%, 84.8%, and 32.5%, respectively. The extraction components in arrowroot's fibers was shown high in 1%-NaOH extraction. The amount of holocellulose and lignin were largely decreased, but a slight decrease was shown in the bleaching processes. Many shivers were shown in the grounded fibers. The amount of shivers was decreased and the single fibers were increased during the process of bleaching. The alkaline pulp and the alkaline bleach pulp showed high in the beating time and freeness than the grounded pulp and grounded bleach pulp. The conditions of sheet surface according to beating time is effected largely by the conditions of original pulp.