• International Journal of Oncology
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• 제54권4호
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• pp.1327-1336
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• 2019

Endothelial progenitor cells (EPCs) are bone marrow (BM)-derived progenitor cells that can differentiate into mature endothelial cells, contributing to vasculogenesis in the blood vessel formation process. Runt-related transcription factor 3 (RUNX3) belongs to the Runt domain family and is required for the differentiation of specific immune cells and neurons. The tumor suppressive role of RUNX3, via the induction of apoptosis and cell cycle arrest in a variety of cancers, and its deletion or frequent silencing by epigenetic mechanisms have been studied extensively; however, its role in the differentiation of EPCs is yet to be investigated. Therefore, in the present study, adult BM-derived hematopoietic stem cells (HSCs) were isolated from Runx3 heterozygous (Rx3^+/-) or wild-type (WT) mice. The differentiation of EPCs from the BM-derived HSCs of Rx3^+/- mice was found to be significantly increased compared with those of the WT mice, as determined by the number of small or large colony-forming units. The migration and tube formation abilities of Rx3^+/- EPCs were also observed to be significantly increased compared with those of WT EPCs. Furthermore, the number of circulating EPCs, defined as CD34⁺/vascular endothelial growth factor receptor 2 (VEGFR2)⁺ cells, was also significantly increased in Rx3^+/- mice. Hypoxia-inducible factor (HIF)-1α was upregulated in Rx3^+/- EPCs compared with WT EPCs, even under normoxic conditions. Furthermore, in a hindlimb ischemic mouse models, the recovery of blood flow was observed to be highly stimulated in Rx3^+/- mice compared with WT mice. Also, in a Lewis lung carcinoma cell allograft model, the tumor size in Rx3^+/- mice was significantly larger than that in WT mice, and the EPC cell population (CD34⁺/VEGFR2⁺ cells) recruited to the tumor was greater in the Rx3^+/- mice compared with the WT mice. In conclusion, the present study revealed that Runx3 inhibits vasculogenesis via the inhibition of EPC differentiation and functions via the suppression of HIF-1α activity.

• Journal of Ginseng Research
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• 제46권3호
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• pp.426-434
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• 2022

Aim: This study investigates the effects of ginsenoside Rb1 (GsRb1) on methamphetamine (METH)-induced toxicity in SH-SY5Y neuroblastoma cells and METH-induced conditioned place preference (CPP) in adult Sprague-Dawley rats. It also examines whether GsRb1 can regulate these effects through the NR2B/ERK/CREB/BDNF signaling pathways. Methods: SH-SY5Y cells were pretreated with GsRb1 (20 mM and 40 mM) for 1 h, followed by METH treatment (2 mM) for 24 h. Rats were treated with METH (2 mg/kg) or saline on alternating days for 10 days to allow CPP to be examined. GsRb1 (5, 10, and 20 mg/kg) was injected intraperitoneally 1 h before METH or saline. Western blot was used to examine the protein expression of NR2B, ERK, P-ERK, CREB, P-CREB, and BDNF in the SH-SY5Y cells and the rats' hippocampus, nucleus accumbens (NAc), and prefrontal cortex (PFC). Results: METH dose-dependently reduced the viability of SH-SY5Y cells. Pretreatment of cells with 40 µM of GsRb1 increased cell viability and reduced the expression of METH-induced NR2B, p-ERK, p-CREB and BDNF. GsRb1 also attenuated the expression of METH CPP in a dose-dependent manner in rats. Further, GsRb1 dose-dependently reduced the expression of METH-induced NR2B, p-ERK, p-CREB, and BDNF in the PFC, hippocampus, and NAc of rats. Conclusion: GsRb1 regulated METH-induced neurotoxicity in vitro and METH-induced CPP through the NR2B/ERK/CREB/BDNF regulatory pathway. GsRb1 could be a therapeutic target for treating METH-induced neurotoxicity or METH addiction.

• 한국식품위생안전성학회지
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• 제29권4호
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• pp.322-326
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• 2014

본 연구에서는 어린이 기호식품 중 하나인 과자류(과자, 캔디류, 츄잉껌, 빙과류)에 대하여 1,005건(과자 250건, 캔디류 255건, 츄잉껌 250 건, 빙과류 250건)에 대해 위생 지표 미생물인 일반세균 수, 대장균군/대장균과 더불어 식중독 원인균인 바실러스 세레우스, 클로스트리디움 퍼프린젠스 및 황색포도상구균에 대한 미생물 검사를 실시하였다. 과자류에 대한 일반세균 수의 경우 전체 시료의 26.8%에서 검출되었고, 검출된 세균 수는 1.7 log CFU/g을 나타내었다. 유형별로 살펴보았을 때에는 과자 250건 중 45.2%의 시료에서 검출되었으며, 빙과류 28.8%, 캔디류 20.0% 및 츄잉껌 13.2% 순으로 검출되는 것으로 확인되었다. 다른 위생지표 세균인 대장균군/대장균의 경우는 모든 시료에서 검출되지 않았다. 식중독균을 분석한 결과에서는 캔디류를 제외한 나머지 유형에서 바실러스 세레우스만 검출이 되었고, 평균 검출량은 1.4~1.6 log CFU/g로 현행 식품공전상의 미생물규격인 3 log CFU/g 이하 수준으로 모두 적합한 제품이었다. 이는 식품공전 상에서 정하고 있는 미생물 규격에 비해서는 낮은 수치로 식품의 제조 유통에는 문제가 없는 것으로 보이지만, 식중독 민감도가 높은 어린이들에게 안전한 식품을 제공하기 위해 합리적이고 과학적인 미생물 기준규격을 고려해야 한다.

• 약학회지
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• 제43권3호
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• pp.289-293
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• 1999

Many poly (vinyl chloride : PVC) membrane electrodes were investigated for the determination of basic drugs, chlorpromazine, amitriptyline, nortriptyline, etc. These electrodes are based on the use of the ion-association complexes of the basic drugs with eriochrome cyanine R, chromoxane cyanine, chrome azurol S and picric acid as ion-exchange sites in a plasticized PVC matrix. All ion-exchangers except picrate complex were not proper for use, because those complexes in plasticized membrane were excluded into aqueous working solution. These drug electrodes show excellent Nernstian responses in the concentration ranges $10^{-2}~10^{-6}$ mol $dm^{-3}$. Their selectivity with respect to each other, as well as their work-able pH range have been investigated. The major advantages of the proposed methods are their simplicity and speed.

• Journal of Korean Biological Nursing Science
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• 제17권2호
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• pp.188-210
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• 2015

Purpose: The purpose of this study was to develop syllabuses of basic nursing science subjects (Structure and Function of Human Body, Pathogenic Microbiology, Pathophysiology, and Mechanisms and Effects of Drug) based on learning outcomes. Methods: We developed a 3-phase plan to develop the syllabuses. In the first phase, The Korean Society of Biological Nursing Science (KSBNS) held a workshop in May, 2013 in which professors who are in charge of basic nursing science subjects shared opinions about learning outcomes. As a result, initial prototype syllabuses came out. In the second phase, revised syllabuses based on learning outcomes were presented and discussed in the conference held by KSBNS in November, 2013. In the last phase, a research team who taught basic nursing science subjects finalized the syllabuses during the meetings. Results: Syllabuses of 4 basic nursing science subjects were developed. Conclusion: These syllabuses of 4 subjects need to be disseminated throughout nursing colleges. Further revision needs to be made according to the circumstances and context of each school.

• 대한지역사회영양학회지
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• 제16권1호
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• pp.145-154
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• 2011

The purpose of this study was to evaluate the relationship between taste perceptions and risk factors for health of Korean elderly living in rural areas. Recognition thresholds for four basic tastes, drug consumption, BMI, fasting blood glucose, serum total cholesterol, serum triglyceride, systolic blood pressure, and diastolic blood pressure were assessed in 176 males and 312 females aged between 50 and 88 years. For the recognition threshold of the four basic tastes, alcohol drinking did not influence their sensitivities, but the alcohol drinking group preferred a higher pleasant concentration of NaCl than did the non-alcohol drinking group. However, smoking significantly decreased sensitivities of the four basic tastes. For the pleasant concentration of NaCl, the smoking group tended to prefer a higher concentration than the non-smoking group. Drug consumption, fasting blood glucose, serum total cholesterol, and serum triglycerides did not have a significant correlation to the sensitivity of the four basic tastes and preference of salty solution. Systolic blood pressure and diastolic blood pressure may have been positively correlated with the pleasant concentration of NaCl but did not correlate with the recognition thresholds of NaCl and sucrose. Further, systolic blood pressure was negatively correlated with the recognition thresholds of caffeine, whereas diastolic blood pressure was negatively correlated with the recognition thresholds of caffeine and citric acid. The finding that the risk factors for health correlated with taste perception has diagnostic and practical implications for health promotion for the elderly.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Current Trends in Toxicological Sciences
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• pp.24-42
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• 2002

Antioxidant responsive element (ARE) mediates the transcriptional activation of the genes encoding phase II drug metabolizing enzymes and antioxidative stress genes. The ARE consensus sequence shows high similarity to NF-E2 binding sequence, a cisacting erythroid gene regulatory element.(omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-1
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• pp.25-25
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• 2003

“Roundup BioKorea” is the project to develop the role of Korea Food and Drug Administration in leading the process of the effective materials from basic research work to licensed commercial BioProducts. Korean government has invested a large amount of budget to a biotechnological area by “Biotech 2000” program since 1994. (omitted)

• 한국식품위생안전성학회지
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• 제34권1호
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• pp.13-21
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• 2019

본 연구는 2018년 국내식품 중 신규 기준설정 예정 농약인 스트렙토마이신의 잔류 농약 검사를 위한 공정시험법을 마련하기 위하여 수행하였다. 스트렙토마이신은 아미노글리코사이드계 항생제로 소, 돼지, 닭 등 가축 및 가금에 동물용의약품으로 허가되어 세균성 질병치료에 사용되고 있다. 또한 우리나라에서는 감귤, 매실, 참다래, 복숭아 등에 발병하는 세균성 병해를 방지하기 위하여 농약으로 등록되어 있다. 국내에서는 스트렙토마이신의 잔류물의 정의를 스트렙토마이신과 디히드로스트렙토마이신의 합으로 정하여 관리하고 있으며 잔류허용기준은 동물용의 약품으로 사용 시 불검출부터 1.0 mg/kg까지 설정되어 있다. 농약으로 사용 시 농산물 중 잔류허용기준은 설정되어 있지 않으며 식품공전의 시험법 역시 동물용의약품에 적용되는 시험법으로 설정되어 있다. 이에 농산물 중 잔류허용기준 설정을 위한 시험법 마련이 시급하여 본 연구에서는 식품공전에서 정하고 있는 대표농산물 5종(현미, 감자, 대두, 감귤, 고추)을 포함한 잔류시험법을 개발하고자 하였다. 따라서 수용성 유기용매인 메탄올의 적용과 포름산을 이용한 pH 조절을 통한 추출법 및 HLB 카트리지를 이용한 정제법을 최적화하여 LC-MS/MS에 의한 분석법을 확립하였다. 스트렙토마이신과 디히드로스트렙토마이신의 시험법 정량한계는 0.01 mg/kg으로 PLS 제도에 맞는 잔류허용기준 수준을 만족하였다. 스트렙토마이신과 디히드로스트렙토마이신의 평균 회수율은 각각 72.0~116.5%와 72.1~116.0%이었고, 상대표준편차는 각각 12.3%와 12.5% 이하로 조사되어 이러한 결과는 국제식품규격위원회 가이드라인(CAC/GL 40-1993, 2003)의 잔류농약 분석기준 및 식품의약품안전평가원의 '식품등 시험법 마련 표준절차에 관한 가이드라인(2016)'에 적합한 수준임을 검증하였다. 따라서 본 연구에서 개발한 시험법은 농산물 중 스트렙토마이신의 잔류검사를 위한 공정시험법으로 활용할 수 있으며 유사 농산물에 대한 적용도 가능하리라 판단되어 향후 국내 농산물 중 농약의 잔류허용기준 신설 및 잔류농약 검사의 기초자료로 활용 가능할 것이다.

• IMMUNE NETWORK
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• 제1권1호
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• pp.7-13
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• 2001

Currently 18 monoclonal antibodies were approved by FDA for inj ection into humans for therapeutic or diagnostic purpose. And 146 clinical trials are under way to evaluate the efficacy of monoclonal antibodies as anti-cancer agents, which comprise 9 % of clinical trials in cancer therapy field. When considering a lot of disappointment and worries existed in this field during the past 15 years, this boom could be called as resurrection. Antibodies have several merits over small molecule drug. First of all it is easier and faster in development, as proper immunization of the target proteins usually raises good antibody response. The side effects of antibodies are more likely to be checked out in immunohistomchemical staining of whole human tissues. Antibody has better pharmacokinetics, which means a longer half-life. And it is non-toxic as it is purely a "natural drug. Vast array of methods was developed to get the recombinant antibodies to be used as drug. The mice with human immunoglobulin genes were generated. Fully human antibodies can be developed in fast and easy way from these mice through immunization. These mice could make even human monoclonal antibodies against any human antigen like albumin. The concept of combinatorial library was also actively adopted for this purpose. Specific antibodies can be screened out from phage, mRNA, ribosomal library displaying recombinant antibodies like single chain Fvs or Fabs. Then the coding genes of these specific antibodies are obtained from the selected protein-gene units, and used for industrial scale production. Both $na\ddot{i}ve$ and immunized libraries are proved to be effective for this purpose. In post-map arena, antibodies are receiving another spotlight as molecular probes against numerous targets screened out from functional genomics or proteomics. Actually many of these antibodies used for this purpose are already human ones. Through alliance of these two actively growing research areas, antibody would play a central role in target discovery and drug development.