• Asian-Australasian Journal of Animal Sciences
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• 제30권7호
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• pp.1029-1036
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• 2017

Objective: In the livestock industry, the regulatory mechanisms of muscle proliferation and differentiation can be applied to improve traits such as growth and meat production. We investigated the regulatory pathway of MyoD and its role in muscle differentiation in quail myoblast cells. Methods: The MyoD gene was mutated by the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 technology and single cell-derived MyoD mutant sublines were identified to investigate the global regulatory mechanism responsible for muscle differentiation. Results: The mutation efficiency was 73.3% in the mixed population, and from this population we were able to establish two QM7 MyoD knockout subline (MyoD KO QM7#4) through single cell pick-up and expansion. In the undifferentiated condition, paired box 7 expression in MyoD KO QM7#4 cells was not significantly different from regular QM7 (rQM7) cells. During differentiation, however, myotube formation was dramatically repressed in MyoD KO QM7#4 cells. Moreover, myogenic differentiation-specific transcripts and proteins were not expressed in MyoD KO QM7#4 cells even after an extended differentiation period. These results indicate that MyoD is critical for muscle differentiation. Furthermore, we analyzed the global regulatory interactions by RNA sequencing during muscle differentiation. Conclusion: With CRISPR/Cas9-mediated genomic editing, single cell-derived sublines with a specific knockout gene can be adapted to various aspects of basic research as well as in functional genomics studies.

• 한국동물생명공학회지
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• 제35권2호
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• pp.113-118
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• 2020

Hepatic stellate cells (HSCs) play essential roles in normal and pathophysiological function in liver. In steady state, HSCs contribute to retinoid storage, immune tolerance, and extracellular matrix (ECM) homeostasis. Upon liver injury, they become activated and lead to morphological and functional changes. Studies have demonstrated that activation of HSCs by various stimuli such as toxins, microbial infection, or metabolic overload can promote the fibrotic changes in liver by production of ECM. Herein, we provide current knowledge about the basic characteristics of HSCs and the mechanism by which they are activated.

• Asian-Australasian Journal of Animal Sciences
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• 제18권2호
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• pp.170-178
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• 2005

An in silico approach was developed to survey the genes expressed in four internal organs of pig: liver, kidney, spleen and small intestine. The major procedures of the approach included: (1) BLAST searching against GenBank "est_others" database using human cDNA sequences as queries to screen the porcine orthologous expressed sequence tags (ESTs), (2) classifying the porcine ESTs records by resources according to certain criteria and (3) analyzing data for ESTs specifically expressed in each organ. In order to do so, four Java programs were developed. Based on the ESTs available in the GenBank database, it was found that there were at least 2,100 genes expressed in these four organs, including 128 in the liver, 81 in the kidney, 780 in the spleen, and 1,423 in the small intestine respectively (a few genes co-expressed in these tissues). Gene expression patterns, such as co-expressed genes, preferentially expressed genes and basic active genes were also compared and characterized among these organs. This study provides a comprehensive model on how to use the bioinformatics approach and Genbank databases to facilitate the discovery of new genes in livestock species.

• Animal Bioscience
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• 제34권2호
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• pp.256-264
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• 2021

Objective: The purpose of this experiment was to investigate the effects of the Agaricus bisporus stem residue (ABSR) on the performance, nutrients digestibility, antioxidant activity of laying hens, and its effects on egg storage to determine the appropriate dosage of ABSR, so as to provide a scientific basis for the effective utilization of ABSR. Methods: A total of 384 53-wk-old Nongda III layers were randomly divided into six treatments, four replicates in each treatment and 16 birds in each replicate. The control treatment was fed with basic diet, while experimental treatments were fed with diets of 2%, 4%, 6%, 8%, and 10% ABSR respectively. The experimental period was 56 d. Results: The results showed that compared with the control treatment, ABSR had no significant effect on laying performance (p>0.05). The crude protein and total energy digestibility of experimental treatments was significantly higher than those of control treatment (p<0.05). When eggs were stored for 1 wk, 2 wk, and 3 wk at 25℃, there were no significant differences in egg storage between the experimental treatments and the control treatment (p>0.05). The superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) activity in the serum of the experimental treatments were significantly higher than those of the control treatment (p<0.05), and the malonaldehyde (MDA) content did not change dramatically. SOD activity in yolk of experimental treatments was significantly higher than that in control treatment (p<0.05); MDA content in yolk was markedly lower than that in control treatment (p<0.05). The activity of GSH-Px and SOD in yolk of experimental treatments was significantly higher than that of control treatment stored at 25℃ for 21 d, and the content of MDA was significantly lower than that of control treatment (p<0.05). Conclusion: ABSR can be used to improve the antioxidant activity of laying hens without affecting laying performance.

• 한국농공학회논문집
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• 제64권4호
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• pp.65-72
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• 2022

For sustainable development in the construction industry, blast furnace slag has been used as a substitute for cement in concrete. In contrast, ferronickel slag, which is the by-product generated during smelting to ferronickel used in the manufacturing of stainless steel and nickel alloys, has a limitation to use as a binder and an aggregate due to its expansive characteristics. Recently, stabilization technology of ferronickel slag has been improved and studies have been carried out to utilize ferronicke slag as fine aggregate in concrete. Therefore, in this study, basic mechanical properties of concrete used in ferronickel slag aggregate was evaluated. The compressive strength (24, 30, 40 MPa) and replacement rate of ferronickel slag aggregate (0, 10, 25, 50%) were considered as experimental variables. As a result of test, concrete replaced fine aggregate with 25% ferronickel slag aggregate showed superior performance in the compressive strength and flexural strength.

• 농촌계획
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• 제11권4호
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• pp.99-108
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• 2005

In this study, thirteen villages in Chonnam province were selected as case study sites and the spatial distribution of the facilities in the villages was examined to provide basic information fur the establishment of rural plans. According to the questionnaire survey, various problem such as environmental pollution, position, scene, management etc. was brought owing to cattle shed, and dissension was more or less seen by scale of facilities, management of facilities and waste, regional factor(stock farming management condition, life style and attitude of inhabitants) and topographical factor (height, position physical aspect of a mountain, distance with water resources etc.) etc.. The facilities could be classified into 6 types based on the their spatial locations: 1) Type 1, facilities located at the waterside; 2) Type 2, facilities located at the entrance of village; 3) Type 3 facilities, scattered in the residential area: 4) Type 4 facilities, collectivized in village; 5) Type 5 facilities, adjoining village; and 6) Type 6 facilities, scattered irregularly inside and outside of village. Based on the classification, possible implementations for the reduction of environmental impacts were suggested. The results of this study could be used as an example of study on the distribution, classification, and rearrangement of environment-impacting facilities in rural areas for improvement of their roles in providing amenity resources.

• 농업과학연구
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• 제31권1호
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• pp.35-44
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• 2004

In this study, the automatic system exchanging well-plates was developed as a basic stage of the genome project. The developed system consisted of the plate fixing well-plates, the well-plate cassette, the head to move a well-plate from the well-plate cassette to the plate fixing well-plates before genome work or from the plate to the cassette after the work, the manipulator to move the head on the X, Y and Z axes and the control system. The performance test to exchange well-plates with the robotic system developed was carried out. The time to set an well-plate from the well-plate cassette onto the board fixing well-plates was 55 seconds and the time for 9 ones was 8 minutes and 15 seconds. It took 57 seconds to move a well-plate from the board to the cassette and 8 minutes and 33 seconds for 9 ones.

• 한국포장학회지
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• 제29권3호
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• pp.211-221
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• 2023

This study was conducted as a basic study for the selection of tags suitable for logistics management in the palletized unit-load unit and the development of various technologies to activate the palletized unit-load shipment of agricultural products through local APCs. Three types of passive RFID tags of UHF 900 MHz and one type of active RFID tag of 2.4 GHz band designed and manufactured through this study were used to analyze the receiving strength according to the tag's attachment location and distance of the palletized unit-load of agricultural products. In the passive RFID tag, there was a large difference in receiving strength by the tag's attachment location and a large amount of data loss depending on the distance within 30 m, whereas, in the active RFID tag, it was superior to the passive tags in terms of both receiving strength and data loss. Therefore, active tags are desirable from the perspective of multiple identification of warehouses with large spaces in relation to the application of RFID tags for palletized unit-loads of agricultural products, but the development of low-power technologies such as software wakeup power management as well as hardware to minimize battery power consumption is necessary.

• Advances in robotics research
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• 제2권3호
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• pp.201-217
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• 2018

Agriculture production is a critical human intensive task, which takes place in all regions of the world. The process to grow and harvest crops is labor intensive in many countries due to the lack of automation and advanced technology. Much of the difficult, dangerous and dirty labor of crop production can be automated with intelligent and robotic platforms. We propose an intelligent, agent-oriented robotic team, which can enable the process of harvesting, gathering and collecting crops and fruits, of many types, from agricultural fields. This paper describes a novel robotic organization enabling humans, robots and agents to work together for automation of gathering and collection functions. The focus of the research is a model, called HARMS, which can enable Humans, software Agents, Robots, Machines and Sensors to work together indistinguishably. With this model, any capability-based human-like organization can be conceived and modeled, such as in manufacturing or agriculture. In this research, we model, design and implement a technology application of knowledge-based robot-to-robot and human-to-robot collaboration for an agricultural gathering and collection function. The gathering and collection functions were chosen as they are some of the most labor intensive and least automated processes in the process acquisition of agricultural products. The use of robotic organizations can reduce human labor and increase efficiency allowing people to focus on higher level tasks and minimizing the backbreaking tasks of agricultural production in the future. In this work, the HARMS model was applied to three different robotic instances and an integrated test was completed with satisfactory results that show the basic promise of this research.

• 농촌계획
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• 제6권2호
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• pp.3-11
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• 2000

The high salt concentration of reclaimed tidelands in the beginning of reclamation interferes with the growth of most crops. Although the crops are cultivated in the unripened tidal reclaimed paddy fields after desalinization to be arable, they we apt to be injured from salt by the resalinization through accumulated salts in the root zone during the growing period. In oder to make the reasonable irrigation plan in the unripened tidal reclaimed paddy fields, the preventive water requirements of resalinization as well as leaching requirements have to be included in irrigation water requirements. The critical salinity for the normal growth of crops should be determined to estimate the preventive water requirements of resalinization, and the changes of salinity in soil and water should be analyzed during the growing period, In this study, the growth tests of crops were conducted by soil textures and water management methods in the experimental field with lysimeters, using the samples of good drainage soils and poor drainage soils. And the changes of salinity in soil and water during the growing period, were analyzed to obtain the basic data for determining the critical salinity and making the estimation criteria of the preventive water requirements of resalinization. As the results obtained from analyzing the changes of salinity during the growing period in the unripened tidal reclaimed paddy fields, the exchanging interval of water for the prevention of resalinization was estimated to be within two weeks in good drainage soils and a week in poor drainage soils. And the total exchanging requirements of water for the prevention of resalinization during the growing period was estimated to be over 280mm in good drainage soils and 540mm in poor drainage soils.