• Korean Journal of Agricultural Science
• /
• v.28 no.2
• /
• pp.78-84
• /
• 2001

This study was analyzed by PCR technique with specific primer in order to investigate the characteristics of ${\beta}$-lactoglobulin (${\beta}$-LG) gene 5'flanking region in Korean native goat. This work confirmed amplified product of 1,077 bp fragments obtained from the amplification of ${\beta}$-LG promoter from genomic DNA using PCR in Korean native goat. The nucleotide sequence of ${\beta}$-LG gene 5'flanking region in Korean native goat as compared with that of sheep ${\beta}$-LG were different at 46 base of 897 nucleotides, and showed high homology as about 94.9% each breed. Especially we confirmed that the difference of nucleotide sequences between Korean native goat and sheep were consisted of $T{\rightarrow}C$ substitution and $C{\rightarrow}T$ substitution reversely. As a consequences, the sequences of ${\beta}$-LG gene 5'flanking region showed a high homology between Korean native goat and sheep. Furthermore we should be studied that relationships between the control of gene expression and nucleotide sequences of transcription factor in Korean native goat.

• Journal of the Korean Chemical Society
• /
• v.31 no.4
• /
• pp.352-358
• /
• 1987

Rate constants for the hydrolysis of para-substituted phenyl N-(p-chlorobenzoyl)chloroformimidate (I) derivatives in 1 : 4 dioxane-water at $25^{\circ}C$ have been determined. Rate data, substituent effect $(\rho\>{\rho}^+)$, product analysis and MO calculation indicate that the uncatalyzed reaction proceeds through an $S_N1$ mechanism involving the formation of azocarbonium ion (II) below pH 3.0, and the base-catalyzed reaction proceeds through an $S_N2$ mechanism via transition state (III) above pH 4.0. The relative stability of four peri planar conformational isomers were (E-ap) > (Z-ap) > (E-sp) > (Z-ap), respectively, and the most stable stereo structures shows that the Y-substituted phenyl group $(C_6H_4-Y)$ occupy vertical $(90^{\circ})$ position on the plane of the benzimidochloroformyl group in (E-ap) conformer. The nucleophilic substitution of water molecule occurs by sigma attack to the activatived azomethine carbon atom of (I) derivatives.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.7
• /
• pp.2023-2028
• /
• 2013

A kinetic study is reported for the nucleophilic substitution reactions of phenyl Y-substituted-phenyl carbonates (5a-5k) with piperidine in 80 mol % $H_2O$/20 mol % DMSO at $25.0{\pm}0.1^{\circ}C$. The plots of $k_{obsd}$ vs. [piperidine] for the reactions of substrates possessing a strong electron-withdrawing group (EWG) in the leaving group (i.e., 5a-5i) are linear and pass through the origin. In contrast, the plots for the reactions of substrates bearing a weak EWG or no substituent (i.e., 5j or 5k) curve upward, indicating that the electronic nature of the substituent Y in the leaving group governs the reaction mechanism. Thus, it has been suggested that the reactions of 5a-5i proceed through a stepwise mechanism with a zwitterionic tetrahedral intermediate (i.e., $T^{\pm}$) while those of 5j and 5k proceed through a stepwise mechanism with two intermediates (i.e., $T^{\pm}$ and its deprotonated form $T^-$). The slope of the Br${\o}$nsted-type plot for the second-order rate constants (i.e., $k_N$ or $Kk_2$) changes from -0.41 to -1.89 as the leaving-group basicity increases, indicating that a change in the rate-determining step (RDS) occurs. The reactions of 5a-5k with piperidine result in larger $k_1$ values than the corresponding reactions with ethylamine.

• The Journal of Engineering Geology
• /
• v.14 no.2
• /
• pp.189-197
• /
• 2004

For a long time, groundwater has been used for a substitution for surface water but recently many problems have risen due to shortage of water resources and decrepitude of waterwells. Pneumatic fracturing technique is likely to be an efficient way to solve the problem of tile wells, in which pressure under the ground is applied to increase the amount of ground water. When applied pressure is given artificially to unstabilize the rock stress or to remove substances between fractures the groundwater can inflow. As the air pressure applied on the base rocks is stronger, permeability is getting higher, thus producing much groundwater than ever before. The result of this study show 15% increase of pumping rate in the P-5 well. After pneumatic fracturing pumping rate changed from 26m3/day to 30m3/day, drawdown rate increase from 51.12m to 56.58m, and specific yield also increased from 0.51m3/day to 0.53m3/day.

• Structural Engineering and Mechanics
• /
• v.61 no.2
• /
• pp.245-253
• /
• 2017

The increasing lack of good quality soils allowing the development of roadway, motorway, or railway networks, as well as large scale industrial facilities, necessitates the use of reinforcement techniques. Their aim is the improvement of the global performance of compressible soils, both in terms of settlement reduction and increase of the load bearing capacity. Among the various available techniques, the improvement of soils by incorporating vertical stiff piles appears to be a particularly appropriate solution, since it is easy to implement and does not require any substitution of significant soft soil volumes. The technique consists in driving a group of regularly spaced piles through a soft soil layer down to an underlying competent substratum. The surface load being thus transferred to this substratum by means of those reinforcing piles, which illustrates the case of a piled embankment. The differential settlements at the base of the embankment between the soft soil and the stiff piles lead to an "arching effect" in the embankment due to shearing mechanisms. This effect, which can be accentuated by the use of large pile caps, allows partial load transfer onto the pile, as well as surface settlement reduction, thus ensuring that the surface structure works properly. A technique for producing rigid piles has been developed to achieve in a single operation a rigid circular pile associated with a cone shaped head reversed on the place of a rigid circular pile. This technique has been used with success in a pile-supported road near Bourgoin-Jallieu (France). In this article, a numerical study based on this real case is proposed to highlight the functioning mode of this new technique in the case of industrial slabs.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.11
• /
• pp.4589-4592
• /
• 2015

Background: Missense and frame-shift mutations within the dimer forming domain of the caspase 8 gene have been identified in several cancers. However, the genetic status of this region in precancerous lesions, like oral submucous fibrosis (OSMF), and well differentiated oral squamous cell carcinomas (OSCCs) in patients from southern region of India is not known, and hence the present study was designed to address this issue. Materials and Methods: Genomic DNA isolated from biopsy tissues of thirty one oral submucous fibrosis and twenty five OSCC samples were subjected to PCR amplification with intronic primers flanking exon 7 of the caspase 8 gene. The PCR amplicons were subsequently subjected to direct sequencing to elucidate the status of mutation. Results: Sequence analysis identified a frame-shift and a novel missense mutation in two out of twenty five OSCC samples. The frame-shift mutation was due to a two base pair deletion (c.1225_1226delTG), while the missense mutation was due to substitution of wild type cysteine residue with phenylalanine at codon 426 (C426F). The missense mutation, however, was found to be heterozygous as the wild type C426C codon was also present. None of the OSMF samples carried mutations. Conclusions: The identification of mutations in OSCC lesions but not OSMF suggests that dimer forming domain mutations in caspase 8 may be limited to malignant lesions. The absence of mutations in OSMF also suggests that the samples analyzed in the present study may not have acquired transforming potential. To the best of our knowledge this is the first study to have explored and identified frame-shift and novel missense mutations in OSCC tissue samples.

• Transactions of the Korean hydrogen and new energy society
• /
• v.10 no.4
• /
• pp.197-210
• /
• 1999

The hydrogen storage performance and electrochemical properties of $Zr_{1-X}Ti_X(Mn_{0.2}V_{0.2}Ni_{0.6})_{1.8}$(X=0.0, 0.2, 0.4, 0.6) alloys are investigated. The relationship between discharge performance and alloy characteristics such as P-C-T characteristics and crystallographic parameters is also discussed. All of these alloys are found to have mainly a C14-type Laves phase structure by X-ray diffraction analysis. As the mole fraction of Ti in the alloy increases, the reversible hydrogen storage capacity decreases while the equilibrium hydrogen pressure of alloy increases. Furthermore, the discharge capacity shows a maxima behavior and the rate-capability is increased, but the cycling durability is rapidly degraded with increasing Ti content in the alloy. In order to analyze the above phenomena, the phase distribution, surface composition, and dissolution amount of alloy constituting elements are examined by S.E.M., A.E.S. and I.C.P. respectively. The decrease of secondary phase amount with increasing Ti content in the alloy explains that the micro-galvanic corrosion by multiphase formation is little related with the degradation of the alloys. The analysis of surface composition shows that the rapid degradation of Ti-substituted Zr base alloy electrode is due to the growth of oxygen penetration layer. After comparing the radii of atoms and ions in the electrolyte, it is clear that the electrode surface becomes more porous, and that is the source of growth of oxygen penetration layer while accelerating the dissolution of alloy constituting elements with increasing Ti content. Consequently, the rapid degradation (fast growth of the oxygen-penetrated layer) with increasing Ti substitution in Zr-based alloy is ascribed to the formation of porous surface oxide through which the oxygen atom and hydroxyl ion with relatively large radius can easily transport into the electrode surface.

• Biomedical Science Letters
• /
• v.4 no.2
• /
• pp.137-142
• /
• 1998

We examined mRNAs, isolated from the rat brain, to ascertain if there is any polymorphism for S-100 beta protein gene. As templates for polymerase chain reaction (PCR) the reverse-transcribed cDNA from the rat brain or phage DNAs isolated from the rat brain cDNA libraries were used. Although PCR products turned out to be exactly same as the expected size based on the previously reported mRNA sequence a single base substitution (CAT to CAC) was identified at nucleotide level. This change was considered as polymorphism since it did not cause any change of the primary structure for S-100 beta protein. This result should facilitate the understanding of the overall structure of the gene for S-100 beta protein.

• Applied Chemistry for Engineering
• /
• v.5 no.3
• /
• pp.431-437
• /
• 1994

The role and the formation of surface and bulk acid sites of heteropoly acids were studied by examining ethanol conversion and MTBE (methyl t-butyl ether) decomposition reaction. In ethanol dehydration diethylether was formed on the surface acid site of 12-tungstophosphoric acid, whereas ethylene was formed in the bulk acid site of the catalyst. It was revealed that water reinforced the bulk acid site of the catalyst, while organic base decreased the bulk acid function of the catalyst. The formation of acid sites of metal salts was due to hydrolysis of crystalline water and/or partial substitution of metal, and with hydrogen treatment, the acid site was reappeared. Also catalyst design as a selective oxidation catalyst was possible by controlling acid function of heteropoly acid catalyst.

• Smart Media Journal
• /
• v.5 no.4
• /
• pp.103-110
• /
• 2016

This study aims to establish the standards to give the design weight in case when developing equipment designs, by classifying products with similar purposes in accordance with the morphological characteristics and also extracting the typicality. Limiting the sampling group for the extraction of the typicality to a pendant-type and a ceiling buried-type through the preceding case study and consultation with experts, the survey was conducted for majors and relevant workers in six cities, sampling products released in Korea and Japan before May 2016. The 1st survey was about the Morphological classification, and the 2nd times about the extraction of the typicality while the 3rd one was about the classification of the morphological attributes. By drawing the design attributes based on the functional/morphological classification and formative principles, it aimed to establish the base of the future research on the measurement of design weight. The results of this study aim to efficiently establish the roles of design technology in accordance with changes in the lighting fixture market caused by the substitution of light sources, and also to draw the development direction to rapidly cope with the accelerated changes in lighting design types and corporate R&D.