• Food Science and Preservation
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• v.16 no.6
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• pp.938-945
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• 2009

The objective of this study was to investigate the effects of ingestion of low-molecular-weight collagen peptides on lipid composition, blood glucose level, and enzyme activities in the serum of hyperlipidemic rats fed experimental diets for 5 weeks. Concentrations of total cholesterol, low-density lipoprotein (LDL)-cholesterol, free cholesterol, triglyceride (TG), phospholipid (PL), and blood glucose, the atherosclerotic index, and the cholesteryl ester ratio were higher in serum of the hyperlipidemic group (CW group), and the cholesterol-plus-collagen peptides extract group (CCP group) than in the control group (BG group basal diet plus water). However, the concentrations of total cholesterol, LDL-cholesterol, free cholesterol, TG, PL, and blood glucose, the atherosclerotic index, and the cholesteryl ester ratio in serum were lower in the CCP group than in the CW group. By contrast, the ratio of HDL-cholesterol to total cholesterol and the absolute HDL-cholesterol level in the CCP group were higher than in the CW group. The activities of alkaline phosphatase (ALP) and aminotransferases (AST, ALT) in serum were lower in the CCP group than in the hyperlipidemic CW group. The results indicate that an extract of low-molecular-weight collagen peptides effectively inhibited increases in lipid elevation, blood glucose level, and enzyme activities, in the serum of hyperlipidemic rats.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.3
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• pp.369-378
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• 2018

Objective: The impact of forage feeding strategy on growth performance, ruminal fermentation and nutrient digestibility in post-weaning calves was investigated. Methods: Forty-five female Holstein calves (body weight [BW] = $79.79{\pm}0.38kg$) were enrolled in the 35-d study at one week after weaning and randomly assigned to one of three dietary treatments. All diets were fed as total mixed ration containing 60% (dry matter [DM] basis) of basal starter feed and 40% (DM basis) of forage, but varied in composition of forage source including i) alfalfa (40% DM, AH); ii) alfalfa hay (26.7% DM)+oat hay (13.3% DM; OH); iii) alfalfa hay (26.7% DM)+corn silage (13.3% DM; WS). Results: Dry matter intake was not different among treatment groups (p>0.05). However, BW (p<0.05) and average daily gain (p<0.05) of calves fed AH and OH were greater than WS-fed calves, whereas heart girth was greater in OH-fed calves than those fed AH and WS (p<0.05). Ruminal fermentation parameters including proportion of butyric acid, acetated-to-propionate ratio, concentration of total volatile fatty acid, protozoal protein, bacterial protein, and microbial protein in rumen were the highest in OH (p<0.05) and the lowest in WS. Compared with the AH and WS, feeding oat hay to postweaning calves increased crude protein digestibility (p<0.05), and decreased duration of diarrhea (p<0.05) and fecal index (p<0.05). Conclusion: Our results suggested that partially replacing alfalfa hay with oat hay improved ruminal fermentation, nitrogen utilization, and reduced incidence of diarrhea in post-weaning dairy calves.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.11
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• pp.1588-1596
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• 2006

An experiment was conducted to study the effect of different dietary protein levels on the performance, nutrient balances, blood biochemical parameters and thyroid hormones of crossbred calves. Thirty crossbred (Bos taurus${\times}$Bos indicus) calves aged 3-5 months were divided into 3 equal groups of 10 each and fed graded levels of crude protein, namely 100 (NP), 75 (LP) and 125 (HP) percent of the Kearl recommendations for 105 d. The calves had access to ad libitum oat hay as the basal roughage. A metabolism trial of 6 d duration was conducted at 90 d of the study. Blood collection and its analysis for various hematological and biochemical parameters as well as thyroid hormones was done both during the pre- and post-experimental periods. The fortnightly body weight changes and the net gain did not differ significantly due to dietary variation. The average daily gain was $367{\pm}21.6$, $347{\pm}22.9$ and $337{\pm}26.4g$ in calves fed NP, LP and HP diets, respectively. Averaged across the feeding trial, oat hay intake was higher (p<0.05) in NP animals than HP or LP fed groups. The dry matter (DM) intake showed no significant difference between the 3 groups but the DM digestibility was higher (p<0.05) in the HP fed animals. The digestibility of crude protein, organic matter, crude fiber and nitrogen-free extract was significantly higher (p<0.05) on HP diets compared to LP or NP diets. The calves on all 3 diets were in positive nitrogen (N) balance, however the N retention was higher (p<0.05) in HP than in LP fed calves. The intake and retention of calcium and phosphorus were similar between the treatments. The blood biochemical profile revealed no significant influence of the dietary treatments on hemoglobin, packed cell volume as well as serum levels of glucose, total protein, albumin, globulin, Ca, P, and alkaline phosphatase. Serum levels of the circulating thyroid hormones ($T_3$ and $T_4$) tended to be lower (p>0.05) on feeding of the LP diet besides showing an increasing trend with the advancement of age. Considering the similar performance and metabolic profile, it could be concluded that crossbred calves can be satisfactorily reared on 25% lower protein level as recommended by Kearl for developing countries, which would not only economize the cost of production but also help to reduce environmental pollution attributable to livestock production.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.1
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• pp.74-81
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• 2010

The effects of different levels of natural clay enterosorbent on the growth, nutrient availability, and genital organs of post-weaning female pigs fed with an addition of zearalenone (ZEA) were investigated in the study. A total of thirty-five post-weaning gilts ($L{\times}Y{\times}D$) with an average body weight of 12.36${\pm}$1.46 kg were used in the test. The gilts were raised individually in metabolism cages and fed a corn-soybean meal-whey basal diet with an addition of 0 or 1 mg/kg of ZEA for 24 d with four levels of natural clay enterosorbent added in the feed. The treatments were: i) control; ii) control+2.5 g/kg clay; iii) control+1 mg/kg ZEA; iv) control+1 mg/kg ZEA+1.25 g/kg clay; v) control+1 mg/kg ZEA+2.5 g/kg clay; vi) control+1 mg/kg ZEA+5.0 g/kg clay; vii) control+1 mg/kg ZEA +10 g/kg clay. Pigs fed diets contaminated with additional purified ZEA had significantly reduced apparent digestibility of crude protein (CP), gross energy (GE) and apparent metabolic rate of GE (ME/GE, p<0.05) without changes of net protein utilization (NPU, p>0.05). Final body weight, average daily gain (ADG), average daily feed intake (ADFI), vulva length, vulva width, vulva area, relative weights of genital organ and proliferative changes of the ovary tissues in gilts fed ZEA-contaminated diet were increased (p<0.05) compared to the gilts fed the control diet. Addition of natural clay enterosorbent in the ZEA-contaminated diet showed a positive protection effect on ZEA feeding, and the protection was increased linearly or quadratically as clay content increased. However, in pigs fed a diet with clay alone at 2.5 g/kg level there was no significant impact (p>0.05) on all the parameters as compared to the control. It is suggested that feeding ZEA at about 1.0 mg/kg for 24 days might result in a deleterious effect in pigs, and addition of 5 or 10 g clay enterosorbent per kg diet can effectively neutralize the detrimental effects of the ZEA feeding.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2002.11a
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• pp.100-102
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• 2002

This study was conducted to determine the effect of feeding betaine on egg production, lipid metabolism and osmoregulation in laying hens. Three hundred and sixty layers were fed corn-soy basal diet containing 16% CP, 2800 kcal/kg ME from 18 to weeks. Hens were provided with betaine 0, 300, 500 or 1200 ppm. Egg production, feed intake, egg weight and feed conversion were examined in every four weeks. Liver and egg betaine. serum cholesterol and neutal fat were measured at the mid experiment. Egg production was not statistically different among treatments from 18 to 26 weeks of age, but significantly increased for the rest of period in hens fed more than 600ppm betaine(P＜0.05). Betaine added treatments showed a tendency to increase feed intake. Feed conversion tended to improve from 26 to 33 weeks old in betaine supplemental groups. Egg qualify was not influenced by dietary supplemental betaine. Betaine supplementation seemed to increase in liver betaine. but not in egg betaine. Serum total cholesterol, triacylglycerol, abdominal fat content and ileal osmolality were not different. However, liver fat content was prone to decrease in betaine supplemental groups. The result of this experiment suggested that dietary supplemtal betaine can improve the egg production and decrease liver fat content.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.7
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• pp.937-945
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• 2010

This study aimed to investigate the effects of dietary zinc (Zn), environmental temperatures and Zn${\times}$temperature interaction on growth, feathering score and mineral composition of broilers. A total of 256 d-old Avian male broiler chicks were randomly allocated to a $4{\times}2$ factorial arrangement with four corn-soybean meal basal diets (containing 44 mg Zn/kg) supplemented with 0, 40, 60 mg/kg Zn (Diets 1, 2 and 3, respectively; 0.8% Ca for these three diets) and non-Zn supplementation, 1.6% Ca (Diet 4) and two temperature conditions (low: 26, 24, $22^{\circ}C$ vs. high: 30, 28, $26^{\circ}C$). All birds were given feathering coverage scores for back, breast, wing, under-wing and tail. The wing and tail were further evaluated for the occurrence and severity of defect feathers. Feathers were then pooled for mineral composition analysis. The results showed that in high temperature conditions, broilers fed Zn-unsupplemented, 0.8% Ca ration (Diet 1) had significantly (p<0.05) lower ADFI and ADG (wk 1-6) than birds under low temperature conditions. However, when the birds were fed 40 and 60 mg/kg Zn supplementation (Diets 2 and 3), the ADFI and ADG in both temperature conditions were not significantly different. In low temperature conditions, the ADFI, ADG (p<0.05), all feather coverage (p<0.01) and tail defect scores (p<0.001) of birds fed Diet 4 (excess Ca) were significantly poorer than those fed Diet 1. More Ca (p<0.05) was retained in the feathers of broilers fed Diet 4 under high temperature conditions. Broilers fed the Zn-unsupplemented ration (Diet 1) had significantly higher feather phosphorus (p<0.01) and potassium (p<0.05) concentrations than those fed the 60 mg/kg Zn-supplemented ration (Diet 3). A reduction of feather phosphorus (p<0.01) and potassium (p<0.05) and higher manganese (p<0.05) concentrations were observed in Diet 4 broilers as compared to those fed Diet 1. Under high temperature conditions, broilers had lower iron (p<0.05) and higher manganese (p<0.05) concentrations in feathers. Broilers kept in high temperature conditions had a higher Zn requirement and 40 mg/kg Zn supplementation was sufficient for the birds to achieve optimum growth. Supplemental Zn ameliorated the adverse effect of high temperature on growth and occurrence of tail feather defects. Excess Ca disrupted Zn metabolism to exert a detrimental effect on growth performance and normal feathering and this was elucidated in the birds kept in low temperature conditions.

• Journal of Nutrition and Health
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• v.4 no.1
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• pp.49-62
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• 1971

한국인(韓國人)의 기초대사량(基礎代謝量)과 1일소비열량(日消費熱量)을 측정(測定)하기 위하여 Douglas씨(氏) ${\ulcorner}$빽${\lrcorner}$과 Scholander씨(氏) ${\ulcorner}$가스${\lrcorner}$ 분석기(分析器)를 사용(使用)하여 넓은 연령(年齡), 직종(職種), 활동(活動)의 영역(領域)에 긍(亘)하여 실험(實驗)을 행(行)하였다. 938 예(例)의 기초대사측정치(基礎代謝測定値)는 Boothby, Fleish, Robertson and Reid 등(等)의 표준치(標準値)와 근사(近似)하였다. 겨울에는 기초대사량(基礎代謝量)은 9.9%의 증가(增加)를 또한 수면중(睡眠中)의 대사량(代射量)은 기초대사량(基礎代謝量)의 약(約) 94%이었다. 또한 Sanborn Metabulator를 사용(使用)하여 1,000 명(名) 이상(以上)의 기초대사량(基礎代謝量)을 측정(測定)하였다. 또한 여러 가지 동작(動作) 활동중(活動中)의 소비열량(消費熱量)이 측정(測定)되었다. 하루의 전소비열량(全消費熱量)은 $3{\sim}7$일간(日間)에 긍(亘)하여 하루 24시간(時間)의 활동상황(活動狀況)을 분단위(分單位)로 기록(紀錄), 집계(集計)하고 이것과 기초대사량(基礎代謝量) 및 개별활동시(個別活動時)의 소비열량(消費熱量)을 참작(參酌), 계산(計算)하였다. 수면중(睡眠中)의 소비열량(消費熱量)은 기초대사량(基礎代謝量)에 준(準)하였는데 사무원(事務員), 교원(敎員), 의사(醫師), 약사(藥師), 점원(店員), 운전수(運轉手), 건축기사(建築技師), 설계사(設計師), 응원사(應園師), 목공(木工), ${\ulcorner}$펜기${\lrcorner}$ 공등(工等)의 1일소비열량(日消費熱量)은 FAO 한국협회책정(韓國協會策定)의 한국인(韓國人) ${\ulcorner}$칼로리${\lrcorner}$에 근사(近似)하였고 ${\ulcorner}$쿡${\lrcorner}$, ${\ulcorner}$웨이터${\lrcorner}$, 농부(農夫), 사공(士工), 기계공(機械工), 석공(石工)은 중등정도(中等程度)의 노동(勞動)의 3,000 ${\ulcorner}$칼로리${\lrcorner}$나 또는 중(重)한 노동(勞動)의 3,900 ${\ulcorner}$칼로리${\lrcorner}$에, 가정주부(家政主婦), 하녀(下女), ${\ulcorner}$디자이너${\lrcorner}$는 여자(女子)의 중등정도(中等程度) 노동(勞動)의 2,200 ${\ulcorner}$칼로리${\lrcorner}$, 간호원(看護員), 청소부(淸掃婦), ${\ulcorner}$쿡${\lrcorner}$ 방(房)바닥연마공(硏磨工)은 중(重)한 노동(勞動)의 2,800${\ulcorner}$칼로리${\lrcorner}$에 모심기 하는 날의 농부(農夫)는 격심(激甚)한 노동(勞動)의 4,100${\ulcorner}$칼로리${\lrcorner}$에 각각(各各) 가까웠다. 여자(女子)일지라도 포제용(鋪製用) 돌까기와 돌날르기는 남자(男子)의 중노동정도(重勞動程度)의 ${\ulcorner}$칼로리${\lrcorner}$를 소비(消費)하였다.

• Journal of Chest Surgery
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• v.13 no.2
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• pp.85-91
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• 1980

Extracorporeal circulation by hemodilution technique has been currently used with its clinical safety and good peripheral tissue perfusion in open heart surgery. There is no doubt that $O_{2}$ carrying capacity of the blood is disturbed by decreased hemoglobin level resulting from hemodilution of the circulating blood. From the view point of the blood gas exchange, these experimental studies were undertaken to determined the sate limit of hemodilution in the condition of cardiopulmonary bypass with a constant perfusion flow rate. Twelve adult mongrel dogs weighing 10 to 13 Kg. were anesthetized with pentobarbital and then respiration was controlled with Harvard volume respirator using room air. The cardiopulmonary by pass was performed by use of Sarns heart lung machine (console 5000, 5 head and 2 roller pumps) and Travenol pediatric bubble oxygenator. The perfusion rate during bypass was maintained at a constant rate of 80 ml/min/Kg of body weight. The ratio of oxygen gas flow to blood flow was kept in 3 to 1 constantly. International hemodilution was attained by serial blood withdrawals and immediate infusion of equal volumes of diluants composed of Ringer's lactate, 5% dextrose in water and 25% mannitol solution, proportionally 60%, 30%, and 10%. Arterial and venous blood samples were obtained between 15 and 20 minutes following each hemodilution. Hematocrits and hemoglobin values, $PO_{2}$, $PCO_{2}$ and pH were measured. Oxygen and carbon dioxide contents oxygen consumption and carbon dioxide elimination were calculated groups according to different hematocrit values and the correlations were evaluated. Result were as follows. 1. the arterial $O_{2}$ tension and $O_{2}$ saturation were maintained at the physiological level irrespective of the hematocrit value. 2. The venous $O_{2}$ tension and $O_{2}$ saturation showed a tendency to decline with the decrease in hematocrit value and positive correlation between them (r = +0.49, r = +0.76), The mean values of venous $O_{2}$ tension and $O_{2}$ saturation, however, were not decreased when the hematocrit levels were lower than 20%. 3. The arterial $O_{2}$ content declined lineally in proportion to the fall of hematocrit level with a positive correlation between them (r = +0.95). 4. The venous $O_{2}$ contents were decreased gradually as the hematocrit value decreased with positive correlation between them ( r =+0.89). The trend of diminution of venous $O_{2}$ content, however, was became low according to progressive decrease of hematocrit level. 5. Systemic oxygen consumption was in higher range than $O_{2}$ requirement of basal metabolism when the hematocrit value was above 20%, but abruptly decreased when the hematocrit value became to below 20%. 6. The arterial $CO_{2}$ tension and $CO_{2}$ content showed trend of increasing with progressive decrease of hematocrit value but exhibited a rather broad range and there was no relationship between those value and the hematocrit value. 7. The venous $CO_{2}$ tension and $CO_{2}$ content have also no correlation with change of Ht. value but related directly to those value of arterial blood with positive correlation between them (r = +0.78, r = +0.95_. 8. A-V difference of $CO_{2}$ content and $CO_{2}$ elimination wasnot significantly influenced by Ht. value. From the results, we obtained that feasible limit in inteneional hemodilution is above the hematocrit value of 20% under the given experimental condition.

• Korean Journal of Clinical Laboratory Science
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• v.48 no.4
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• pp.296-303
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• 2016

This study was performed to evaluate the effect of hemoglobin (Hb) on bone mineral density (BMD) in university students by performing a quantitative analysis. The subjects included healthy university students aged 20 to 30 years. Although osteoporosis has traditionally been considered as a disease of aging women, it is becoming an increasingly concerning male health problem. Diagnosis of osteoporosis is calculated with a quantitative assessment of BMD. Laboratory blood and urine tests are mainly used with low BMD or fragility fractures to identify any possible causes of bone metabolism disorders. In this study, there was no difference in BMD according to gender. The average red blood cell (RBC), Hb, and Hematocrit (HCT) were significantly higher in males (p<0.01). The correlation between lumbar spine, skeletal muscle mass (SMM), and basal metabolic rate (BMR) was statistically significant (p<0.01). Hb showed a 51.7% statistical influence on BMD by multiple regression analysis. These findings are useful to understand the relationship between BMD and Hb; lower Hb level is associated with lower BMD. The Hb level was the strongest predictor of abnormal BMD. In conclusion, this study showed that a low Hb value was significantly correlated with low bone mass, suggesting that a low Hb value is a risk factor for changes in bone turnover that leads to a decrease bone density.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.3
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• pp.236-244
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• 2018

Taurine has been reported to prevent cardiovascular disease and improve liver function, diabetes, and platelet function. However, there are few studies on the effects of taurine in Koreans. The purpose of this study was to investigate the effect of basal doses of taurine on blood glucose, liver disease, and lipid diseases among Korean college students. The study included a taurine intake group and a control group; each group consisted of 15 students. Taurine was administered at a standard dose of 1,000 mg for 2 weeks postprandial. All subjects were excluded from medication or other food besides meals provided by the dormitory. The liver test gamma-glutamyl transferase (GGT) in the taurine group decreased to $23.53{\pm}25.73IU/L$ before intake and to $15.15{\pm}4.91IU/L$ after intake (P=0.186). Lipid metabolite triglyceride (TG) was $100.42{\pm}28.33mg/dL$ before intake and $80.22{\pm}17.08mg/dL$ after intake (P<0.05). Total cholesterol (T-Cho), low density cholesterol (LDL-C), and high density lipoprotein cholesterol (HDL-C). Consequently, taurine improved liver function and lipid metabolism. Hematologic tests showed a decrease in segmented neutrophil percentage and an increase in lymphocyte percentage. Thus, taurine also seems to be related to immunological function.