• 한국동물학회지
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• 제24권2호
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• pp.65-75
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• 1981

Gerris paludum의 精子形成 동안의 尖體形成에 대한 연구를 요약하면 다음과 같다. 1. Golgi體는 精母細胞의 초기단계에서 細胞質에 산재되어 있다가 서로 융합하여 囊 胚形을 하며 이들이 모여서 감수분열 전기에는 몇 개의 큰 Golgi體群을 형성하는데, 이들은 결국 精細胞에 균등히 분포된다. 2. 精子完成에서 acroblast는 처음에 하나의 큰 胞로 나타나다가, 그 내부에서 尖體顆粒이 分化된 후, acroblast는 顆粒에서 分離되어 결국 tail filament를 따라 사라진다. 3. 尖體는 mitochondrial derivatives의 반대편인 核 전면부로 이동한 후, two zones 즉 core와 sheath로 分化된다. 4. Basal bodies와 tip은 모두 sheath에서 由來한다. Basal bodies는 sheath의 基部에서 발생해서 尖體가 伸長되고 좁아짐에 따라 점차로 尖體의 基部를 싸기 시작하여 결국 완전히 둘러싸게 되며, 分化된 tip은 core의 前端部에 인접해서 나타나지만 sheath와 뚜렷이 연결되어 있다. 5. 分化된 tip은 basal bodies보다 앞서서 伸長한다. Basal bodies는 精細胞 후기에 하나의 顆粒으로 융합하지 않고 서로 연결된 wtin-tubes로 나타나며, sperm bundle에서는 basal bodies group을 형성한다.

• 환경생물
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• 제38권2호
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• pp.278-288
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• 2020

Rhodomonas salina is a phototrophic marine flagellate. We examined the ultrastructure of R. salina with particular attention to the flagellar apparatus by transmission electron microscopy and compared it with that of other cryptomonads reported previously. The major components of the flagellar apparatus in R. salina were a keeled rhizostyle (Rhs), a striated fibrous root(SR), a SR-associated microtubular root (SRm), a mitochondrion-associated lamella (ML), and three types of microtubular roots (9r, 4r, and 2r). The keeled Rhs originated near the proximal end of the dorsal basal body, passed near the nucleus and dissociated at the posterior end of the cell. The SR and SRm originated between two basal bodies and laterally extended to the right side of the cell. The ML originated between two basal bodies and extended to the left side of the cell. The 9r originated between the ventral basal body and the Rhs and extended toward the anterior dorsal lobe of the cell. The 4r originated near the 9r and extended toward the dorsal lobe with the 2r, which originated between two basal bodies. Here, the flagellar apparatus in R. salina is described, and the ultrastructure of the flagellar apparatus is compared among cryptomonad species.

• ALGAE
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• 제28권4호
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• pp.331-341
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• 2013

Rhinomonas reticulata var. atrorosea G. Novarino is a photosynthetic marine flagellate that is known to have typical characteristics of cryptomonads. We examined the flagellar apparatus of R. reticulata var. atrorosea by transmission electron microscopy. The major components of the flagellar apparatus of R. reticulata var. atrorosea consisted of four types of microtubular roots (1r, 2r, 3r, and mr), a non-keeled rhizostyle (Rhs), mitochondrion-associated lamella (ML), two connections between basal bodies, a striated fibrous root (SR) and a striated fiber-associated microtubular root (SRm). Four types of microtubular roots originated near the ventral basal body and extended toward the left side of the basal bodies. The non-keeled Rhs originated at the Rhs-associated striated fiber, which was located between two basal bodies and extended into the middle of the cell. The ML was a plate-like fibrous structure associated with mitochondria and originating from a Rhs-associated fiber. It split into two parts and extended toward the dorsal-posterior of the cell to a mitochondrion. The SR and SRm extended parallel to the anterior lobe of the cell. The overall configuration of the flagellar apparatus in R. reticulata var. atrorosea was similar to the previously reported descriptions of those of Cryptomonas paramecium, C. pyrenoidifera, C. ovata, Hanusia phi, Guillardia theta, and Proteomonas sulcata. However, the flagellar apparatus system of R. reticulata var. atrorosea was more complex than those of other cryptomonad species due to the presence of an additional microtubular root and other distinctive features, such as a rhizostyle-associated striated fiber and large ML.

• Applied Microscopy
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• 제25권1호
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• pp.48-64
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• 1995

Ultrastructure of nerves and their associated cells in the bronchiolar epithelium of the human fetal lung were studied with ultrastructural and immunohistochemical methods. The neuroendocrine cells were scattered along the basal part of non-ciliated respiratory epithelium and appeared as single cell (solitary neuroendocrine cell) or groups (neuroepithelial bodies). The solitary neuroendocrine cells were devoid of any detectable innervation, while the neuroepithelial bodies were associated with nerve ending containing morphologically afferent (sensory) and efferent (motor) intraepithelial terminals. The afferent nerve endings contained abundant mitochondria with long cristae. The efferent nerve endings were characterized by the presence of synaptic vesicles. Both types of nerve endings formed synaptic junction between nerve endings and neuroepithelial bodies cells. Serial sections of the intraepithelial nerves revealed that both morphologically afferent and efferent types of nerve endings may be formed by the same nerve fiber. By immunohistochemistry, bombesin and serotonin were localized in solitary neuroendocrine cells and neuroepithelial bodies of human fetal lung from various prenatal age groups. These results suggest that the neuroepithelial bodies cells of the human fetal lung have neuroreceptor function.

• ALGAE
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• 제31권2호
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• pp.117-128
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• 2016

Cryptomonas curvata Ehrenberg is a photosynthetic freshwater flagellate and the type species of the genus Cryptomonas. We examined the flagellar apparatus of cryptomorphic C. curvata by transmission electron microscopy. The major components of the flagellar apparatus are the non-keeled rhizostyle (Rhs), striated fibrous root (SR), striated fiber-associated microtubular root (SRm), mitochondrion-associated lamella (ML), and two types of microtubular roots (3r and 2r). The non-keeled Rhs originate at the ventral basal body and consist of two types of microtubule bands extending together into the middle of the cell. The SR and SRm extend parallel to the left side of the cell. The ML originates from the ventral basal body and is a plate-like fibrous structure associated with mitochondria. The 3r extends from the dorsal basal body toward the dorsal anterior of the cell. The 2r originates between the two basal bodies and extends shortly to the left of the cell. The overall configuration of the flagellar apparatus is most similar to that previously reported for C. pyrenoidifera. These results demonstrate that the features of the flagellar apparatus are useful for distinguishing closely related species and inferring phylogenetic relationships among taxa.

• 화훼연구
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• 제17권1호
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• pp.9-14
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• 2009

밀토니아의 기내 대량생산을 위해 PLB의 유도와 증식을 위한 적정 배지 및 배양방법을 찾고자 본 실험을 수행하였다. 적정 배지는 MS 기본배지가 Hyponex 또는 VW배지보다 밀토니아의 PLB 유도가 우수하였다. PLB 증식 및 신초분화는 Hyponex 고체배지(Hyponex +바나나 20 g/L + sucrose $20g{\cdot}L^{-1}$, pH 5.2)가 MS 배지에 비해 더욱 효과적이었다. PLB로부터 신초분화에는 sucrose를 $10{\sim}20g{\cdot}L^{-1}$ 첨가하는 것이 효과적이었으나 30 g 이상의 농도에서는 유도된 신초수가 크게 감소하였다.

• 한국석유지질학회:학술대회논문집
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• 한국석유지질학회 2000년도 제7차 학술발표회 발표논문집
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• pp.47-56
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• 2000

The northwestern part of Kyongsang Basin largely comprises interbedded sandstone and mudstone with local conglomeratic deposits in the basin margin, representing marginal alluvial fans and fluvial depositional environments. The non-marine successions are divided into successive stratigraphic units, each of which is unique in facies assemblages and architecture of sandstone bodies. Two stratigraphic units, i.e., Sinpyong-Anpyong and Jotap units are examined in terms of stratigraphic architecture and its causative processes. Detailed architectural analysis reveals that the channel systems of Sinpyong-Anyong unit were of braided patterns, whereas those of Jotap unit were dominated by small-scale bedforms. The difference in fluvial styles can be attributed to the changes in amount and caliber of sediment load and water discharge, which might have been ultimately governed by basin tectonics, climate, and base level. Along with the marked change in fluvial style, the two successive units show repeated expansions of distal, water-logged floodplains and lacustrine facies in the basal and uppermost parts of Sinpyong-Anpyong unit, where the proportion of channel sandstone bodies is relatively low. These stratigraphic intervals are succeeded by the sequences with proximal, well-drained floodplain facies and relatively coarser-grained channel sandstone bodies of higher proportion, reflecting the progradation of proximal systems (the middle part of Sinpyong-Anpyong unit and Jotap unit). The overall stratigraphic architecture can be ascribed to the fluctuations in accommodation space and sediment supply induced by repeated basin subsidence.

• 대한수의학회지
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• 제34권4호
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• pp.715-725
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• 1994

The ultrastructural investigations of the parotid gland of Korean native goat were carried out by transmission electron microscopy. The results were as follows; 1. The acini of parotid gland were composed of light and dark acinar cells. 2. In the light acinar cells, the secretory granules were classified into three types according to their electron densities and dense bodies. One type of granules was low electron density and had no dense bodies. Another type was low electron density and had dense bodies, and the other type was low electron density and had granular dense bodies. 3. The secretory granules of dark acinar cells showed high electron density and were also calssified into three types by dense bodies as the same way as in the light acinar cells. 4. The intercalated ducts consisted of simple cuboidal epithelium. The nuclei of epithelial cells were oval or round form, located at the central part, and had infolding nuclear membranes and one or two nucleoli. 5. The cells of both of the striated and excretory ducts were composed of light cells, dark cells, specific light cells and basal cells. 6. The nerve terminals were distinguished into two types. One had large granular synaptic vesicles, and another had small agranular synaptic vesicles.

• 한국가금학회지
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• 제28권3호
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• pp.267-274
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• 2001

부화후 성장하는 꿩 송과샘의 미세구조적 발달을 구명하고자 1일령, 1, 2 및 6개월령을 희생시켜 광학 및 전자현미경을 이용하여 다음과 같은 결과를 얻었다. 광학현미경적 관찰에서 꿩 송과샘의 실질은 피막에 의해 싸여 있고 여기에서 기시하는 결합조직에 의해 불완전한 소엽 (lobule)으로 구분되었다. 송과샘실질은 소엽상과 불완전한 여포살이었으며, 둥근 핵과 염색성이 옅은 송과샘세포와 약간 짙고 길쭉한 핵을 갖는 지주세포로 구성되어 있었다. 전자현미경적 관찰에서 송과샘실질에는 길쭉한 송과샘세포와 지주세포가 소엽의 중심부를 향해 배열되어 있었고 광학현미경에서 소엽의 중심부에는 매우 불규칙한 막성층판복합체이나 포상구조물이 출현하였다. 실질은 비교적 밝은 솔과 샘세포와 짙은 핵을 갖는 지주세포로 구성되어 있었다. 송과샘세포는 잔유성 광수용세포의 형태를 취하였는데, 그 첨단부위의 밝고 팽대된 세포질에는 세포소기관이 빈약하나 섬모, 약간의 유리리보솜과 사립체가 출현하였다. 첨단부위세포질과 핵위부위세포질 사이의 좁아진 경부는 지주세포와 연접복합체를 이루고 목부위세포질내에는 미세소관이 풍부하였다. 핵주위세포질은 풍부하고 다수의 사립체, 잘 발달된 골지장치, 풍부한 과립형질내세망 및 유리 리보솜 등을 함유하고 있었다. 또한 핵아래부위세포질에서 여포의 기저부위로 기저돌기를 내고 있었다. 송과샘세포의 핵아래부위 세포질 및 기저돌기에서 60∼90nm 크기의 치밀소포가 관찰된 것이 특이하였고 짙은 핵을 갖고 긴 독기를 갖는 것이 특징이었다. 이상의 결과는 꿩 송과샘세포가 광수용능은 것의 없고 분비기능을 주로 수행하는 소견이었으며, 성숙 꿩의 송과샘의 미세구조적 연구를 확인하여 번식기와 비번식기에 따른 비교연구가 수행되어야 할 것으로 사료된다.

• Plant Biotechnology Reports
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• 제4권2호
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• pp.157-163
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• 2010

A protocol for in vitro propagation from pseudobulb sections of Lycaste armomatica (Graham ex Hook) Lindl., an ornamental and fragrant orchid, was developed. The effect of four cytokinins: kinetin (K), metatopolin (mT), $N^6$-benzyladenine (BA), and thidiazuron (TDZ), in equimolar concentrations, was investigated. Shoot formation from apical and basal pseudobulb sections was obtained in all treatments. A few medial sections cultured in media supplemented with BA formed protocorm-like bodies. Shoot formation was greater from the basal section than the apical, and mainly occurred in explants cultured in media containing TDZ. The highest average numbers of shoots per explant were achieved from basal sections cultured in media supplemented with TDZ at 4.4, 8.87 and 2.2 ${\mu}M$, forming on average 9.9, 8.6 and 7.3 shoots per explant, respectively. Since the medial pseudobulb section was the worst explant for propagation of L. aromatica, we recommend that pseudobulbs be divided into two sections; the basal half should be cultured in MS medium supplemented with TDZ at 4.4 ${\mu}M$ and the apical half with TDZ at 2.2 ${\mu}M$. Subculturing individual shoots in MS medium without plant growth regulators allows further development and rooting. A survival rate of more than 90% under greenhouse conditions was achieved. This research represents a direct contribution to the conservation and sustainable use of this valuable natural resource.