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Expression of Mosquitocidal Bacillus sphaericus Binary Toxin and B. thuringiensis cry11B Genes in B. thuringiensis 407

  • Park, Hyun-Woo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.2
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    • pp.185-189
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    • 2001
  • Wild type Bacilus thuringiensis subsp. israelensis and B. sphaericus toxins have been used separately as active in ingredients for bacterial insecticides to control mosquito larvae due to their comparable toxicity to chemical insecticides. Cry11B, recently cloned from B. thuringiensis subsp. jegathesan, shows higher toxicity against three major species of mosquito larvae than Cry11A, one of the major component of B. thuringiensis subsp. israelensis inclusion body. To determine whether the combination of cry11B and B. sphaericus binary toxins is as toxic as B. thuringiensis subsp. israelensis parental strain, cry11B and B. sphaericus binary toxins genes were co-expressed as an operon using cytlA promoters/STAB-SD hybrid expression system in B. thuringiensis subsp. israelensis acrystalliferous strain 4Q7. However, unexpectedly, B. sphaericus binary toxins were barely produced, whereas relatively large amount of Cry11B was produced. When this strain was grown in four different media, NB+G and Peptonized Milk produced more toxin proteins and spores per unit of media than GYS and G-Tris. Toxicity of this strain against fourth instar Culex quinquefasciatus was ranged from of 8.3 to 45.7 ng/ml, with NB+G culture being the highest, and GYS culture was the lowest.

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Comparative of Physicochemical and Sensory Quality Characteristics of Cookies added with Barleys and Oatmeals (보리와 귀리첨가 쿠키의 이화학적 및 관능적 품질특성비교)

  • 이정애;박금순;안상희
    • Korean journal of food and cookery science
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    • v.18 no.2
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    • pp.238-246
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    • 2002
  • The purposes of this study were to provide the basic information on the cookies made with barley and oatmeal powder and to establish an optimum formula for the development of cookies with high content of fiber. Cookie samples were prepared with barley and oatmeal at various mixing ratios (2:0, 1:1, 2:1, 0:2), and the chemical properties, textural characteristics, and sensory properties of them were evaluated. The contents of carbohydrate and moisture of barley cookies were higher than oatmeal cookies, but the contents of crude protein, crude fat, crude ash and total fiber of oatmeal were higher than those of barley. The moisture content was the highest in the cookies of oatmeal and the lowest in barley-used ones. The cookie volume was increased by the addition of barley and oatmeal. The results of sensory evaluation showed that the cookies containing barley and oatmeal (2:1) was significantly more preferable in overall acceptability than the others. Hunter color test showed that the lightness was the highest in control, but the redness and yellowness were increased in the cookies with barley and oatmeal compared with the control.

DEVELOPMENT OF A TOY INTERFEROMETER FOR EDUCATION AND OBSERVATION OF SUN AT 21 cm

  • Park, Yong-Sun;Kim, Chang-Hee;Choi, Sang-In;Lee, Joo-Young;Jang, Woo-Min;Kim, Woo-Yeon;Jeong, Dae-Heon
    • Journal of The Korean Astronomical Society
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    • v.41 no.3
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    • pp.77-81
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    • 2008
  • As a continuation of a previous work by Park et al. (2006), we have developed a two-element radio interferometer that can measure both the phase and amplitude of a visibility function. Two small radio telescopes with diameters of 2.3 m are used as before, but this time an external reference oscillator is shared by the two telescopes so that the local oscillator frequencies are identical. We do not use a hardware correlator; instead we record signals from the two telescopes onto a PC and then perform software correlation. Complex visibilities are obtained toward the sun at ${\lambda}\;=\;21\;cm$, for 24 baselines with the use of the earth rotation and positional changes of one element, where the maximum baseline length projected onto UV plane is ${\sim}\;90{\lambda}$. As expected, the visibility amplitude decreases with the baseline length, while the phase is almost constant. The image obtained by the Fourier transformation of the visibility function nicely delineates the sun, which is barely resolved due to the limited baseline length. The experiment demonstrates that this system can be used as a "toy" interferometer at least for the education of (under)graduate students.

The Design of Rectangular Microstrip Patch Array Antenna with Micorstrip Feed Line Network (마이크로스트립 급전 회로망을 갖는 구형 마이크로스트립패치어레이 안테나의 설계)

  • Park, Doo-Seok;Hong, Ui-Seok;Ko, Young-Hyuk
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.27 no.2
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    • pp.39-46
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    • 1990
  • In this paper, Rectangular microstrip patch array antenna. relative current of which is distributed to 1:2:2:1, is designed and fabricated by using Tschebyscheff's polynominal expression and is verified by theoretical analysis and experiment. Seeking for more accurate resonant frequency, an equivlent network, which is consisted including feed line and power distribution line, is analyzed by using the analysis method of transmission line mode. As a result of their comparison and examination, such as resonant frequency by fringing effects, by return loss, by experiment, it is conformed that errors are not barely and agreed with each other. is also agreed with a measured values.

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Comparison of the Flavor Components of Barley Bran, Barley Meju and Sigumjang (보리등겨, 보리메주 및 시금장의 휘발성 향기성분 조성 비교)

  • Choi, Ung-Kyu;Kwak, Dong-Ju;Son, Dong-Hwa
    • Food Science and Preservation
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    • v.7 no.3
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    • pp.303-307
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    • 2000
  • This study was conducted to investigate difference of the flavor components of barley bran, barley meju and sigumjang. The number of flavor components identified in barely meju and sigumjang was 46, 67 and 61, respectively. Among the flavor components in sigumjang, tetramethylpyrazine was the most dominant and followed by 2-furancarboxaldehyde, ethyl palmitate, 4-ethylphenol. Among the 13 kinds of flavor components commonly identified in thest samples, butanoic acid, hexanoic acid, heptanoic acid, octanoic acid and 2-pentylfuran were the most abundant in barley bran and followed by barley meju and sigumjang. In the mean while the content of nonanoic acid, 2-furancarboxaldehyde, benzenacetaldehyde and tetramethylpyrazine were the most dominant in sigumjang followed by barley meju and barley bran.

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A Study on the Noise and Condensation Characteristics of Complex Structure Drainage Pipe Materials (복합 구조형 배수 배관재의 소음 및 결로 특성에 관한 연구)

  • Kim, Jae-Dol
    • Journal of Power System Engineering
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    • v.20 no.5
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    • pp.72-77
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    • 2016
  • The present study investigates noise and condensation characteristics of polyvinyl chloride (PVC), which is widely used for drainage piping materials, complex double structure by comparing to those of PVC single structure piping materials. In addition, effects of insulation on drainage noise has been measured experimentally. As the results of the experiments, noise reduction effect of PVC complex double structure is superior to that of PVC single structure in terms of elbow and vertical piping materials which are employed for drainage pipes of toilet bowls and bathtub. The insulation barely have effect on the noise reduction in case of the PVC single structure since there is almost no changes in noise occurrence even though the insulation is applied on both elbow and vertical piping materials. Temperature differences between inside and outside of the pipes have been measures for the PVC single and complex double structures as well. In consequence, outside temperature of the PVC complex double structure is higher than that of the PVC single structure. The condensation occurrence time of the PVC complex double structure shows a distinct difference from that of the PVC single structure, thus, the PVC complex double structure has outstanding effect on preventing the condensation.

Multi-dimensional analyses of plant chromosomes and genomes.

  • Fukui, Kiichi;Ohmido, Nobuko;Wako, Toshiyuki
    • Proceedings of the Botanical Society of Korea Conference
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    • 1998.07a
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    • pp.61-70
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    • 1998
  • Genome and chromosome analyses in plants using fluorescence in situ hybridization (FISH) and immuno-staining (IMS) methods are reviewed by presenting the recent results obtained by the Chromosome Link, a group of chromosome and genome researchers. FISH is now effective to detect unique nucleotide sequences with 153 bp on the extended DNA fibers. Genomic in situ hybridization (GISH) also allows painting plant chromosomes of different genomes. GISH is quite effective to detect the genomic differentiation in the individual chromosomes within a nucleus. Three dimensional (3D) analyses are now available by confocal microscopy and a deconvolution system. These techniques are invaluable to visualize both the structural and functional dynamics within a nucleus. 3D-FISH revealed the spatial differentiation of different genomees within a nucleus. 3D-FISH also proved structural partition of centromeric and telomeric domains within a barely nucleus. The dynamic acetylation of histone H4 at the specific regions of a genome during a cell cycle is also analyzed using 3D-IMS. It is anticipated that these methods will provide us powerful tools to understand the structural and functional significance of plant chromosomes and genomes.

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Evaluation of the Strength Properties of Glulam Connections with Inserted Steel Plates and Drift Pins

  • Kim, Ho-Ki;Park, Chun-Young;Lee, Jun-Jae
    • Journal of the Korean Wood Science and Technology
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    • v.36 no.2
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    • pp.29-38
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    • 2008
  • It is well-known that the strength properties of wood connections depend on the density of the wood members, the diameter of the fasteners, the number of fasteners, and the arrangement of the fasteners, etc. In this study, the connection with inserted steel plates and drift pins was made in different configurations. The specimens were Larch and Sugi glulam connections. The specimens were loaded in tension, and the yield loads of the connections were obtained. The values obtained from the tests were compared with the predicted values. Good agreement between the yield loads obtained from the tension failure tests and the predicted yield loads were shown. It was shown that the density of the wood member barely affected the strength properties of the connections. The strength decreases of the Sugi glulam connections by the group effect were less than those of the Larch glulam connections.

An analysis of the characteristics of communicative English classes using COLT (COLT 수업관찰 분석법을 통한 학교 현장의 의사소통중심 영어 수업 분석)

  • Jeon, Young-Joo
    • English Language & Literature Teaching
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    • v.16 no.3
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    • pp.339-363
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    • 2010
  • The purpose of this paper is to investigate how the English classes in secondary schools in Korea are in terms of communicative nature. After literature study, COLT parts A and B were used to analyze three kinds of English classes: a common class type taught by a school teacher who had won 1st prize at TEE contest, a team teaching class taught by NNS and NS, and a model class taught by a pre-service English teacher. Their communicative English classes were videotaped and transcribed for analysis by COLT. The results of the study revealed that students participated in the classes mostly as a unit and, therefore, did not get many meaningful chances to speak English. Second, creative language-use opportunities were barely found during classes. Third, the group work seemed to be prepared before the class, not to be done during the class. Therefore it is likely to be learned from memorization rather than meaningful communication. These results suggest English classes in schools be more communicatively oriented for successful English learning. Further study should be conducted in order to make English classes more communicative to help students communicate their genuine messages in English and to help teachers teach English efficiently.

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The Electrochemical Properties and Mechanism of Formation of Anodic Oxide Films on Mg-Al Alloys

  • Kim, Seong-Jong;Okido, Masazumi
    • Bulletin of the Korean Chemical Society
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    • v.24 no.7
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    • pp.975-980
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    • 2003
  • The electrochemical properties and the mechanism of formation of anodic oxide films on Mg alloys containing 0-15 mass% Al, when anodized in NaOH solution, were investigated by focusing on the effects of anodizing potential, Al content, and anodizing time. The intensity ratio of Mg(OH)₂ in the XRD analysis decreased with increasing applied potential, while that of MgO increased. Mg(OH)₂ was barely detected at 80 V, while MgO was readily detected. The anti-corrosion properties of anodized specimens at each constant potential were better than those of non-anodized specimens. The specimen anodized at an applied potential of 3 V had the best anti-corrosion property. The intensity ratio of the β phase increased with aluminum content in Mg-Al alloys. During anodizing, the active dissolution reaction occurred preferentially in β phase until about 4 min, and then the current density increased gradually until 7 min. The dissolution reaction progressed in α phase, which had a lower Al content. In the anodic polarization test in 0.017 mol·$dm^{-3}$ NaCl and 0.1 mol·$dm^{-3}$ Na₂SO₄ at 298 K, the current density of Mg-15 mass% Al alloy anodized for 10 min increased, since the anodic film that forms on the α phase is a non-compacted film. The anodic film on the α phase at 30 min was a compact film as compared with that at 10 min.