• Journal of Korean Society of Forest Science
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• v.85 no.3
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• pp.453-461
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• 1996

Three Phyllostachys stands of P. pubescens, P. bambusoides and P. nigra var, henonis in Sunchon were studied to investigate biomass, net production and nutrient distribution. Five $10m{\times}10m$ quadrats were set up and 20 sample culms of 2 years and over were harvested for dimension analysis in each stand. One year old culms and subterranean parts were estimated by the harvested quadrat method. The largest mean DBH, height and basal area were shown in P. pubescens stand, and followed by P. nigra var. henonis stand and P. bambusoides stand. There was little difference in accuracy among three allometric biomass regression models of logWt=A+B1ogD, $logWt=A+BlogD^2H$ and logWt=A+BlogD+ClogH, where Wt, D and H were dry weight, DBH and height, respectively. Analysis of covariance showed that there were significant differences in intercept among the linear allometric biomass regressons of three Phyllostachys species. Biomass included subterranean parts was the largest in P. pubescens stand(103.621t/ha), and followed by P. nigra var. henonis stand(86.447t/ha) and P. bambusoides stand(36.767t/ha). Leaf biomass was 6.3% to 7.8% of total biomass in each stands. The ratio of aboveground biomass and subterranean biomass in each stand was 1.87 to 2.26. Net production included subterranean parts was the greatest in P. pubescens stand(6.115t/ha/yr), and followed by P. nigra var. henonis stand(5.609t/ha/yr) and P, bambusoides stand(3.252t/ha/yr). The highest net assimilation ratio was estimated in P. pubescens stand(2.979), and followed by P. nigra var. henonis stand(2.752) and P. bambusoides stand(2.187). Biomass accumulation ratio of each stand was 2.679 to 5.358. Concentrations of N, P and Mg were the highest in leaves, and followed by subterranean parts, and culms+branches in all three species. Concentration of Ca was the highest in leaves, and followed by culms+branches, and subterranean parts in all three species. The difference in biomass among three species stands was caused by their culm size, leaf biomass, net assimilation ratio, and efficiency of leaves to produce culms.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.12
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• pp.1745-1752
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• 2010

This study was conducted to investigate the antimicrobial activity of 70% ethanol (EtOH) extract and the five fractions of the crude extract from Sasa borealis leaves against seven food poisoning bacteria, Staphylococcus aureus, Micrococus luteus, Listeria monocytogens, Bacillus subtilis, Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa. The yield of 70% EtOH extract was 11.4% and those of n-hexane, chloroform, ethyl acetate, n-butanol, and aqueous fractions were 3.0%, 1.1%, 0.6%, 1.3%, and 5.1%, respectively. The 70% EtOH extract and the four fractions except aqueous fraction demonstrated antimicrobial activity against all the seven food poisoning bacteria at a concentration of 0.5%, although it was less compared to benzoic acid. Minimal inhibitory concentration (MIC) of the 70% EtOH extract against all the food poisoning bacteria except S. aureus was $50{\mu}L$/disc. Moreover, chloroform fraction was $35{\mu}L$/disc against 3 food poisoning bacteria and $50{\mu}L$/disc against the other 4 food poisoning bacteria; ethyl acetate fraction was $50{\mu}L$/disc against all the food poisoning bacteria. In addition, n-butanol fraction was $50{\mu}L$/disc against all the food poisoning bacteria except S. aureus. Aqueous fraction, which did not show antimicrobial activity at 5%, was $200{\mu}L$/disc against only S. aureus and L. monocytogen. The 0.25%, and 0.5% of ethyl acetate fraction inhibited the growth of all the food poisoning bacteria 8 to 12 hours and 24 hours, respectively. These results indicate that the Sasa borealis leaves may be useful as a natural antimicrobial substance.

• Journal of Life Science
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• v.26 no.11
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• pp.1330-1335
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• 2016

Sasa species leaves have been used in traditional medicine for their anti-inflammatory, antipyretic, and diuretic properties. Sasa quelpaertensis Nakai is a small bamboo grass that grows only on Mt. Halla on Jeju Island, Republic of Korea. This small bamboo grass has recently been the focus of much attention due to its potential biomass as well as its beneficial health effects. In this study, to promote the efficient utilization of the S. quelpaertensis leaf, we established a simple preparation method for phytochemical-rich extract (PRE) by comparing phytochemical contents and biological activities according to extraction methods. high performance liquid chromatography (HPLC) analysis revealed that the contents of two major phytochemicals such as, tricin (5.35 mg/g) and p-coumaric acid (44.10 mg/g) contained in PRE were higher than those in fresh hot water extract (SQH, p-coumaric 23.39 mg/g, tricin 0.18 mg/g) and ethanol extract (SQE, p-coumaric 10.8 mg/g, tricin 0.38 mg/g). The antioxidant activities [1,1-diphenyl-2-picrylhydrazy (DPPH) radical scavenging activity, 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, nitric oxide (NO) scavenging activity, and xanthine oxidase inhibitory activity] of PRE were higher than those of SQH and SQE. PRE effectively inhibited NO production in LSP- stimulation RAW 264.7 cells, and the growth of human promyelocytic leukemia (HL-60) cells. These results suggest that PRE has a potential as a promising antioxidant and anti-inflammatory agent.

• Food Science and Preservation
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• v.8 no.4
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• pp.469-474
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• 2001

Chemical composition of the water extract of three different kinds of bamboos was investigated to access nutritive sources. Bamboo stalks and leaves were extracted massively and concentrated to 3°Brix, and crude protein and fat contents of the extracts were respectively 0.56∼0.69% and 0.28∼0.58%. Main mineral contents of the extracts were K, P and Mg in order, and those were the highest in Wangdae stalks. In free sugar analysis of the extracts, Maengjongjuk stalks contained 1.1% sucrose, Somdae stalks contained 0.51% fructose and Wangdae stalks contained 0.6% glucose as the highest level. Organic acid contents of the extracts showed high level in acetic and malic acid which were 54.0 and 44.2mg% in extracts of Wangdae stalks respectively. Among amino acids, aspartic acid was contained the highest level in stalk extracts, and their concentrations were 42.2, 39.9 and 34.6mg% in Wangdae, Somdae and Maengjongjuk respectively. In leaf extracts, glutamic acid concentrations were higher than the other amino acids, and those were 19.3, 18.5 and 15.7mg% in Maengjongjuk, Wangdae and Somdae, respectively.

• Journal of Plant Biology
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• v.7 no.2
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• pp.9-13
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• 1964

As to the up-lnd bamboos, I have reported, in my previous works, that Korea has two species in the genus of Pseudosasa, four in Pleioblastus, and other four in Sasa. In the present work, I dealt with Sasamorpha Purpurascens Nakai var. Borealis Nakai which proved the strongest vitality of all up-land bamboo yet found in various up-lands of Korea, and which might be most important from the utility view-point. This report is chiefly on its procreation. Sasamorpha Purpurascens Nakai var. Borealis Nakai can be found almost everywhere throughout the country, and its leaves and stems are much used in farms in various forms of manufacture. It is also welcomed as the feed for livestocks and as cover plants for aforestation, especially as the excellent means of errosion control on devastated hill sides. It is also widely accepted that it will provide, in the immediate future, abundant sources for the up-land bamboo pulp. As the first phase of my research plan on the subject, I undertook to determine the possible best way of its procreation, for which were included the experiments of inducing the growth of subterranean stems, the entire stand cutting, transplanting, reclaimed planting after burning etc. in order to observe the plant elongation, growing conditon, climatical effect etc. What has been fuond out so far given here as follows: 1) Of the various sections of the country, Mt. Odae area gives out the most excellent Sasamorpha Purpurascens Nakai var. Borealis Nakai(the leaf weight of which is 450 gr.) and Taekwanryong area comes the next class. This species can be transplanted anywhere in the South Korea. 2) The elongation of S.P. Nakai var. Borelais begins at around the middle of May and almost completely stops by the 20th of August. 3) The best studied transplanting season is supposed to be Feb. -April, for those transplanted during that period proved 100% of success. The next best transplanting season may be October. Rain fall does not have so much effect on transplanting as the growing season does. 4) In inducing the subterranean stems, the growing season can be ignored. The root expnsion is most animated during the months of April-June, the most active season for water absorption. Those stems induced during the winter proves more rapid growth. 5) The entire stand cutting shows greater growth than the reclaimed planting after burning and that, the growth is most vigorous during May-July, whereas during the hottest months of Aug-Sept. the growth shows only 5 cm. The new shoots grow slower both in the field of entire stand cutting and in the field of reclaimed planting after burning than in the otherwise fields.

• Korean Journal of Food Science and Technology
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• v.23 no.2
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• pp.200-204
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• 1991

Certain parts of 36 kinds of plant were extracted by 75% ethanol and water. The extracts were tested their microbial inhibition activities against several food spoilage microorganisms, Bacillus subtilis, Lactobacillus plantarum, Leuconostoc mesenteroides, Pseudomonas fluorescens, Bacillus cereus and Saccharomyces cerevisiae. The ethanol extract of amur cork was shown inhibitory effect on all 6 species of the microorganisms tested. Chinese pepper, sesame cake, gromwell and oak were on 5 species except S. cerevisiae or P. fluorescens and bamboo leaves, lycopi herba, paulownia and rigida were on 4 species. In general amur cork exhibited the strongest inhibition with a few exceptions on certain species. By disc diffusion method, the ethanol extract of leaf mustard showed the highest inhibition effect on B. subtilis, amur cork on L.plantarum, L. mesenteroides and B. cereus, and amur cork and gallnut on P. fluorescens. Mostly the ethanol extracts in comparison with water extracts showed higher inhibition in most of plants but a few exhibited higher in water extracts.

• The Korea Journal of Herbology
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• v.38 no.4
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• pp.1-9
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• 2023

Objectives : This study aimed to convert into weight the dosages of flower of Daphne canescens and leaf of Phyllostachys nigra var. henonsis, whose daily dosages are likened to an object in 《Treatise on Cold Damage Diseases》. This study also aimed to understand their implications along with the previously studied gypsium and rhubarb. Methods : After reviewing documents, the dosages were converted into weight, confirmed by experiment, and their implications were considered. Results : A chicken-egg-volume of flowers of Daphne canescens is one Ryang(6.5 g) and two handfuls of leaves of Phyllostachys nigra var. henonsis is four Ryang(13 g). Each item was only used once in a decoction. Their dosages were expressed in weight respectively in 《Supplement to the Essential Prescriptions Worth a Thousand Gold》 and 《Bohenggyuljangbuyongyakbubyo》. Gypsium dosage was expressed in weight except in Daecheongryong-tang as chicken-egg-volume(16 Ryang). To liken a dosage to an object is an exception, as in the three cases above; for precision and convenience it is recommended that dosages be expressed in weight, in which case all three items fell into one of eight categories from one Ryang to 16 Ryang. Rhubarb was measured in weight, but only when using the 12-hour leaching method, four Ryang of rhubarb was cut into of 5-6 wide-go-stone-sized pieces and two Ryang into 5-6 go-stone-sized pieces. Conclusions : To liken a dosage to an object is only an exception and lacks precision and convenience; therefore dosages ended up expressed in weight, count, or volume.

• The Korean Journal of Food And Nutrition
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• v.17 no.1
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• pp.18-24
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• 2004

Nutritional characteristics and antioxidation effects of Yakju brewed with natural plants(fine tree leaves, bamboo leaves, ginger, cinnamon and licorice root) were tested. With regard to the contents of phenolic compound, Yakju brewed with natural plants was about 1.7 times higher than Yakju which were not brewed with natural plants. In Yakju brewed with natural plants, lactose content was the highest but maltose was not detected. Minerals were detected in the order of P>K>Mg>Ca>Fe and the content of Fe and Na had a little changes during its 30 days storage. Amino acid contents were getting lower during storage. They were about 28% lower compared 3 days with 30 days storage. Electron donating ability of Yakju and its diluents were tested by DPPH. It was found that the electron donating ability of Yakju was lower as the dilution ratio got higher and as the storage terms got longer. Also, Yakju brewed with natural plants has 95.7∼96.7% of electron donating ability during 30 days storage without regard to its dilution ratio. Superoxide dismutase(SOD) like activity was in inverse proportion to dilution ratio and storage days. The highest SOD like activity was shown 51.5% and 53.9% in 3 days storage Yakju brewed with natural plants and in its 2 times diluents, respectively.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.34 no.4
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• pp.287-301
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• 2008

In order to develop a new depigmenting agent, extracts were obtained from 60 native plants and their antimelanogenic activities were screened by evaluating the inhibitory effect on tyrosinase which is a major enzyme responsibles for the melanin synthesis. The extracts of Trichosanthes kirilowii fruits, Phyllostachys bambusoides inner films (BIF), Clerodendrum trichotomum leaves, and Acer okamotoanum leaves showed relatively high inhibitory effect on tyrosinase and their $IC_{50}$ values were $50{\sim}100{\mu}g/mL$. The extract of BIF inhibited melanin synthesis of B16F10 melanoma cells by 52%, which was the highest among those of various extracts. Furthermore, the effect of BIF extract is 10% higher than that of arbutin (42%), a popular depigmenting agent in Korea. Ten compounds having antimelanogenic activity were isolated from the BIF extract by solvent extraction and chromatography. These compounds were identified as phenolic derivatives: SM701, SM702, SM703, and BPR211 were hydroquinone derivatives; SM707 a gallic acid derivative; SM704, SM705, SM706, SM708 and SM709 ferulic acid derivatives. The free radical scavenging activities of these compounds were measured and compared to those of hydroquinone and vitamin C. The $SC_{50}$ values scavenging 50% DPPH of SM702 and SM709 were $60{\sim}70{\mu}M$ similar to that of hydroquinone and those of SM701 and SM708 were $30{\sim}40{\mu}M$ slightly lower than that of vitamin C. These results suggest the presence of components having high antioxidant activity in the BIF extract. The SM709, identified as 1,2-O-diferulylglycerol, inhibited the activities of tyrosine hydroxylase and dopa oxidase by 18 and 60%, respectively. The SM709 also inhibited the melanin synthesis of B16F10 melanoma cells by 62% and this was the highest antimelanogenic activity among those obtained from the various purified compounds. Therefore, antimelanogenic activity of the BIF extract was concluded to be due to both inhibition of DOPA oxidase and antioxidant activity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.6
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• pp.837-843
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• 2013

Sasa borealis is a major source of bamboo leaves used for traditional medicine in Korea. Obesity is a serious health problem in industrialized countries that has been implicated in various diseases, including type 2 diabetes, hypertension, cancer, and coronary heart disease. Recent reports have proposed mechanisms to reduce obesity by decreasing preadipocyte differentiation, and proliferation in 3T3-L1 preadipocyte. The preadipocytes play a key role by differentiation into mature adipocytes and increasing fat mass. In this study, we investigated whether ethanol-soluble extracts and ethyl acetate-soluble fractions from Sasa borealis inhibits intracellular accumulation of lipid droplets in a dose-dependent manner in 3T3-L1 cells (an important model system for studying adipogenesis). The down-regulation of PPAR${\gamma}$ and C/EBP${\alpha}$ (key adipogenic transcription factors) were confirmed by the reverse transcription polymerase chain reaction (RT-PCR). Ethyl acetate-soluble fractions from Sasa borealis attenuated the expression of PPAR${\gamma}$ and C/EBP${\alpha}$. These results suggest that Sasa borealis inhibits adipogenic differentiation by regulating adipogenic transcription factors in 3T3-L1 cells. Therefore, Sasa borealis extracts may be a good candidate for the management of obesity.