• 제목/요약/키워드: bacterial diarrhea

검색결과 118건 처리시간 0.025초

Effects of a multi-strain probiotic on growth, health, and fecal bacterial flora of neonatal dairy calves

  • Guo, Yongqing;Li, Zheng;Deng, Ming;Li, Yaokun;Liu, Guangbin;Liu, Dewu;Liu, Qihong;Liu, Qingshen;Sun, Baoli
    • Animal Bioscience
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    • 제35권2호
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    • pp.204-216
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    • 2022
  • Objective: The aim of this study was to investigate the effects of dietary supplementation with a multi-strain probiotic (MSP) product containing of Bifidobacterium animalis, Lactobacillus casei, Streptococcus faecalis, and Bacillus cerevisiae on growth, health, and fecal bacterial composition of dairy calves during the first month of life. Methods: Forty Holstein calves (24 female and 16 male) at 2 d of age were grouped by sex and date of birth then randomly assigned to 1 of 4 treatments: milk replacer supplementation with 0 g (0MSP), 2 g (2MSP), 4 g (4MSP), and 6 g (6MSP) MSP per calf per day. Results: Supplementation of MSP did not result in any significant differences in parameters of body measurements of calves during the 30 d period. As the dosage of MSP increased, the average daily gain (p = 0.025) and total dry matter intake (p = 0.020) of calves showed a linear increase. The fecal consistency index of the 2MSP, 4MSP, and 6MSP group calves were lower than that of the 0MSP group calves (p = 0.003). As the dosage of MSP increased, the concentrations of lactate dehydrogenase (p = 0.068) and aspartate aminotransferase (p = 0.081) in serum tended to decrease, whereas the concentration of total cholesterol increased quadratically (p = 0.021). The relative abundance of Dorea in feces was lower (p = 0.011) in the 2MSP, 4MSP, and 6MSP group calves than that in the 0MSP group calves. The relative abundance of Dorea (p = 0.001), Faecalibacterium (p = 0.050), and Mitsuokella (p = 0.030) decreased linearly, whereas the relative abundance of Prevotella tended to increase linearly as the dosage of MSP increased (p = 0.058). Conclusion: The MSP product can be used to reduce the diarrhea, improve the performance, and alter the composition of the fecal bacteria in neonatal dairy calves under the commercial conditions.

환아의 혈액과 변에서 분리된 Shigella flexneri의 생화학적 성상, 항균제 감수성 및 Pulsed-Field Gel Electrophoresis 분석 (Biochemical Characteristics, Antimicrobial Susceptibility and Pulsed-Field Gel Electrophoresis Patterns of Shigella flexneri Isolated from Blood and Fecal Specimens of Pediatric Patients)

  • 김신무;임채원;소향아;심은숙;김은숙;이규식;정윤섭
    • 대한임상검사과학회지
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    • 제39권3호
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    • pp.183-189
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    • 2007
  • Shigellosis is the most common bacterial gastroenteritis both in developing and developed countries, but bacteremia due to Shigella spp. is very rare. In developed countries recent shigellosis is mostly caused by S. sonnei, but S. flexeri infection is rare. We had rare cases of S. flexeri infections in a family in the Jeonbuk Province: an 8-year-old boy with bacteremic shigellosis and 10- and 12-year-old brothers with diarrhea. The isolates had identical biochemical characteristics, and were resistant to ampicillin, chloramphenicol, and co-trimoxazole. PFGE pattern of Not I-restricted genomic DNA suggested that the isolate from blood was closely related to the two strains isolated from stool which had an identical PFGE pattern.

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Brittany Spaniel에서 Proteus mirabilis에 의한 유방염 발생: 증례 (Mastitis with Proteus mirabilis in Brittany Spaniel, a case)

  • 이정원;추금숙;곽길한;고원석;송희종
    • 한국동물위생학회지
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    • 제32권3호
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    • pp.215-218
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    • 2009
  • The dog breeding kennel bacterial infection are very significant in perinatal mortality. In many case, Staphylococcus aureus, Streptococcus, and Escherichia coli were infected in intra-uterine or by the genital tract to the puppies, and they are cause of septicemic death of the puppies and clinical mastitis of bitch, leading to septicemic death of newborn puppies. Severe mastitis due to bacterial infection was diagnosed in a 2 year-old female Brittany Spaniel which loss 6 puppies with odor small rice sharp white diarrhea of nine puppies. Bright curd milk and intestinal sample were inoculated on MacConkey agar, blood agar and brain heart infusion agar, and incubated at $37^{\circ}C$ for 24-48 hrs. Gram negative colonies isolated from these sample which were characterized as Glu, Ure, $H_2S$, Orn, Cit, and Cl, and were identified by Microscan Walk-Aways Baxter, American Type Culture Collection, USA) as Proteus mirabils. The isolate was more sensitive to ampicillin, gentamicin, cefoxitin, cefuroxime, and cefazidime. In this results, we confirmed that cause bacteria of septicemic death in puppies was P. mirabilis.

Enteric Pathogens in Pediatric Patients with Acute Gastroenteritis in Gyeongju, Korea: A Retrospective Study for 7 Years in a Regional Hospital

  • Cho, Seung-Man;Lee, Dong Seok;Ha, Gyoung Yim;Son, Dong-Chul;Lee, Chang Il
    • 한국미생물·생명공학회지
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    • 제47권2호
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    • pp.289-295
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    • 2019
  • Acute diarrhea is a global health problem that causes high morbidity and mortality in children. Notably, enteric pathogen co-infections have been suggested to play an important role in gastroenteritis. In this study, we analyzed 1150 stool specimens of patients who visited the pediatric ward of Dongguk University Hospital in Gyeongju province from January 2011 to December 2017. The average isolation rate of potential stool pathogens over 7 years was 37.3% (429/1150), and coinfections were observed in 51 patients (51/429; 11.9%). In the 51 co-infection cases, the most frequent type of co-infection was found to be that of virus-bacteria (33/51). The most frequently detected bacterial pathogen among the co-infected cases was Clostridium spp. (22/51), out of which Clostridium perfringens was found to be the main pathogen (16/22; 72.7%). Escherichia coli spp. were the second most common bacterial pathogens found in 12 cases (12/51; 23.5%), with 10 cases of E. coli EPEC. Furthermore, the most frequently implicated viral pathogen among the co-infected cases was norovirus (16/51), followed by rotavirus (12/51).

Pathophysiology of enteropathogenic Escherichia coli during a host infection

  • Lee, Jun Bong;Kim, Se Kye;Yoon, Jang Won
    • Journal of Veterinary Science
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    • 제23권2호
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    • pp.28.1-28.18
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    • 2022
  • Enteropathogenic Escherichia coli (EPEC) is a major cause of infantile diarrhea in developing countries. However, sporadic outbreaks caused by this microorganism in developed countries are frequently reported recently. As an important zoonotic pathogen, EPEC is being monitored annually in several countries. Hallmark of EPEC infection is formation of attaching and effacing (A/E) lesions on the small intestine. To establish A/E lesions during a gastrointestinal tract (GIT) infeciton, EPEC must thrive in diverse GIT environments. A variety of stress responses by EPEC have been reported. These responses play significant roles in helping E. coli pass through GIT environments and establishing E. coli infection. Stringent response is one of those responses. It is mediated by guanosine tetraphosphate. Interestingly, previous studies have demonstrated that stringent response is a universal virulence regulatory mechanism present in many bacterial pathogens including EPEC. However, biological signficance of a bacterial stringent response in both EPEC and its interaction with the host during a GIT infection is unclear. It needs to be elucidated to broaden our insight to EPEC pathogenesis. In this review, diverse responses, including stringent response, of EPEC during a GIT infection are discussed to provide a new insight into EPEC pathophysiology in the GIT.

소아에서 급성 인두편도염의 원인에 대한 연구 (Etiology of Acute Pharyngotonsillitis in Children: The Presence of Viruses and Bacteria)

  • 편종석;문경필;강진한;마상혁;배송미
    • Pediatric Infection and Vaccine
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    • 제23권1호
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    • pp.40-45
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    • 2016
  • 목적: 소아에서 급성 인두편도염의 원인균의 분포를 조사하고자 하였다. 방법: 2010년 3월부터 2011년 3월까지 급성 인두편도염 환자의 인두 후부에서 검체를 채취하여 원인바이러스와 세균을 분석하였다. 결과: 615명의 환자 중, 세균이 분리된 경우는 40례(6.5%), 바이러스가 검출된 경우는 310례(50.4%)이었다. 분리된 세균 중 A군 사슬알균이 31례(77.5%)로 가장 많았고, 검출된 바이러스 중에는 adenovirus가 가장 많았으며(203명; 65.5%), 그 다음으로 rhinovirus (65명; 21.0%), enterovirus (43명; 13.9%) 순이었다. 기침, 콧물, 결막염은 바이러스 감염 환자에서, 연구개의 발적과 점상출혈은 세균 감염 환자에서 더 많았다. 결론: 급성 인두편도염 환자에서 바이러스 감염이 세균 감염에 비해 많았으며 임상양상에 차이를 보였다. 따라서 급성 인두편도염 환자에서 무조건적인 항생제의 사용을 피하고 임상양상의 차이를 감안하여 치료를 결정해야 하겠다.

소아설사 환아에서의 Escherichia coli O157 분리 (Isolation of Escherichia coli O157 in Children with Diarrhea)

  • 송원근;김현태;이규만;차재국;이건희
    • Pediatric Infection and Vaccine
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    • 제4권1호
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    • pp.73-78
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    • 1997
  • 목 적 : Escherichia coli (E. coli) O157은 설사, 출혈성대장염 등을 일으키며, 드물게 용혈성 요독증후군까지 일으키는 세균이다. 미국이나 유럽에서는 세균성설사의 2~3번째로 흔한 원인균이다. 우리나라는 이 세균감염이 거의 없는것으로 알려져 왔으나, 우리나라도 점차 햄버거와 같은 간이음식의 섭취가 늘어나, 이 세균감염이 생겼을 가능성이 있을 것으로 사료되어, 향후 이 세균에 대한 통상적인 변배양 검사의 필요성 여부를 알아보고자 하였다. 방 법 : 1996년 3월부터 1997년 2월까지 6개월 이상된 소아설사 환아의 변배양 검체를 대상으로 하였다. Sorbitol-MacConkey 한천에 배양한 후 형성된 집락 중 무색 집락을 대상으로 E. coli로 동정된 것을 E. coli O157 라텍스 응집검사를 실시하여 응집이 되면 양성으로 판독하였다. 결 과 : 총 317 검체중 11월에 1 검체 (0.3%)에서 Shiga 독소를 생성하지 않는 E. coli O157:NM이 분리되었다. 7세 남아로 2일간의 복통과 1일간의 설사 및 구토를 주소로 입원 한 후 특별한 치료없이 2일후 증상이 호전되어 퇴원하였다. 결 론 : 검사된 모든 대변 검체중 한 검체 (0.3%)만이 E. coli O157이 분리되었으나 Shiga 독소를 생성하지 않는 군주였다. 따라서 이 세균의 분리를 위한 통상적인 변배양 검사는 아직 불필요한 것으로 사료되었다.

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대장균의 이열성장독소 생산기전 (Mechanism of Heat-Libile E. coli Enterotoxin Production)

  • 최명식;이광호;장우현;이승훈
    • 대한미생물학회지
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    • 제17권1호
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    • pp.35-41
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    • 1982
  • Enterotoxigenk E. coli is one of the major causative agents of the infantile diarrhea and traveler's diarrhea. The heat-labile enterotoxin is thought to be a virulence factor in the pathogenesis of the diarrhea and to be a marker for identification of the enterotoxigenic E. coli from non pathogenic E. coli. Therefore knowledge about the heat-labile enterotoxin is essential not only for understanding the pathogenesis but also for the diagnosis of the diarrhea. However the in-vitro heat-labile enterotoxin production is reported to be greatly affected by the cultural condition. In this regards, this study was designed to know the optimal conditions for the production of the heat-labile enterotoxin by assaying the permeability factor in the 18 hours culture supernatant of E. coli 08K25(B2) H9 and of E. coli 015 H11. Results obtained were summerized as follows: 1. Amounts of heat-labile enterotoxin produced were greater at initial pH 8.5 than at 7.0 of CYES-2 broth culture. However, the bacterial growth itself was more abundant at 7.0 than at 8.5. 2. Heat-labile enterotoxin per unit volume of culture supernatant was greater at shaking culture than at standing culture condition, but ratio of the enterotoxin produced over the unit mass of E. coli calculated was greater at standing culture than shaking culture condition, indicating that the greater yields of the toxin produced at shaking culture was due to increase in E. coli cell mass compared to the standing culture condition: 3. The enterotoxin produced in the lincomycin(128 microgram/ml) supplemented media was 5 or 11 times greater on the basis of enterotoxin per unit mass of E. coli, compared to the lincomycin-non-supplemented media, indicating that lincomycin itself increases the enterotoxin production. 4. Treatment of 18 hours culture of E. coli with polymyxin B(0.2 mg/ml) for 1 hour increased the yields of enterotoxin amounting to 2 or 5 times of the non-treated control cultures.

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면역유도누에 추출물을 이용한 병원성 대장균의 불활성화 (Inactivation of Pathogenic Escherichia coli Using Crude Extract of Immunized Silkworm)

  • 박종우;정찬영;이창훈;강상국;주완택;김성완;김남숙;김기영
    • 생명과학회지
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    • 제31권8호
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    • pp.755-760
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    • 2021
  • 돼지 설사병은 부종병과 함께 양돈 농가에 막대한 경제적 손실을 입히는 가축질병으로 알려져 있으며, 병원성 대장균의 증식에 의하여 설사 및 대장균 생성 독소에 의한 부종병이 동반된다. 본 연구에서는 항균펩타이드를 포함하는 면역유도누에의 조추출물을 이용하여 돼지 설사 및 부종을 유발하는 F4+ 및 F18ab+ 대장균의 사균체를 제작하고 사균백신으로서 이용가능성을 검토하였다. 면역유도누에 추출물의 항균활성을 환확산법을 통해 분석한 결과 F4+ 및 F18ab+ 대장균에 대하여 높은 활성을 나타내었으며, 평판계수법을 이용하여 대장균의 사균체 생산효율을 분석한 결과 50 mg/ml의 농도를 4℃에서 처리했을 때 가장 높게 나타났다. 또한 제작된 사균체에 대해서 투과전자현미경을 이용하여 분석한 결과 세포질은 유출되고 세포막만이 남아있는 대장균이 확인되었다. 따라서 면역유도누에 추출물을 이용하여 제작된 F4+ 및 F18ab+ 대장균은 부종병 예방을 위한 사균백신으로서 이용가능성이 매우 높을 것으로 판단된다.

Infection Status of Hospitalized Diarrheal Patients with Gastrointestinal Protozoa, Bacteria, and Viruses in the Republic of Korea

  • Cheun, Hyeng-Il;Cho, Shin-Hyeong;Lee, Jin-Hee;Lim, Yi-Young;Jeon, Ji-Hye;Yu, Jae-Ran;Kim, Tong-Soo;Lee, Won-Ja;Cho, Seung-Hak;Lee, Deog-Yong;Park, Mi-Seon;Jeong, Hye-Sook;Chen, Doo-Sung;Ji, Yeong-Mi;Kwon, Mi-Hwa
    • Parasites, Hosts and Diseases
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    • 제48권2호
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    • pp.113-120
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    • 2010
  • To understand protozoan, viral, and bacterial infections in diarrheal patients, we analyzed positivity and mixedinfection status with 3 protozoans, 4 viruses, and 10 bacteria in hospitalized diarrheal patients during 2004-2006 in the Republic of Korea. A total of 76,652 stool samples were collected from 96 hospitals across the nation. The positivity for protozoa, viruses, and bacteria was 129, 1,759, and 1,797 per 10,000 persons, respectively. Especially, Cryptosporidium parvum was highly mixed-infected with rotavirus among pediatric diarrheal patients (29.5 per 100 C. parvum positive cases), and Entamoeba histolytica was mixed-infected with Clostridium perfringens (10.3 per 100 E. histolytica positive cases) in protozoan-diarrheal patients. Those infected with rotavirus and C. perfringens constituted relatively high proportions among mixed infection cases from January to April. The positivity for rotavirus among viral infection for those aged $\leq$ 5 years was significantly higher, while C. perfringens among bacterial infection was higher for $\geq$ 50 years. The information for association of viral and bacterial infections with enteropathogenic protozoa in diarrheal patients may contribute to improvement of care for diarrhea as well as development of control strategies for diarrheal diseases in Korea.