• Title/Summary/Keyword: bacteria isolation

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고온. 호기법에 의한 중화요리잔반의 처리 과정에서의 중.고온균의 분리 및 특성

  • Yang, Jae-Kyung;Seo, Yong-Ki;Choi, Kyung-Min;Park, Eung-Roh;Whang, Key;Lee, Sung-Taik
    • Microbiology and Biotechnology Letters
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    • v.25 no.6
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    • pp.623-629
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    • 1997
  • The studies of isolation and physiological characteristics of mesophilic or thermophilic bacteria from thermophilic oxic process (TOP) treating Chinese restaurant wastes were conducted. Chinese restaurant wastes were consist of moisture; 75.8%, solids; 24.2% and ash; 0.49%. The volatile solid was about 99% of total dry solids. In wastes used in this experiment, there was content of crude protein; 4.47%, crude lipid; 3.56%, free sugar, 0.4% , crude starch; 10.34% and crude fiber 3.14%, respectively. And then it has about 4,970 kcal/kg-dry solid of Chinese restaurant wastes. From TOP treating the chinese restaurant wastes, 37 strains of mesophilic or thermophilic bacteria were primarily isolated using medium used for the isolation and among them 6 strains of thermophilic and 7 strains of mesophilic bacteria were selected by testing the activities of amylase, cellulase, protease and lipase. TB-1, TB-9 as thermophilic bacteria and MB-15-1, 15-2, MB23 as mesophilic bacteria having strong enzyme activity were selected among isolated strains. All selected strains reduced nitrate to nitrite and they utilized glucose, manose, manitol, and maltose as carbon source. From these MB15-2 was identified as Bacillus cereus, TB1, Bacillus licheniformis and TB9; Bacillus schlegelii.

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Marine Bacteria Associated with the Korean Brown Alga, Undaria pinnatifida

  • Lee, Yoo-Kyung;Jung, Hyun-Jung;Lee, Hong-Kum
    • Journal of Microbiology
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    • v.44 no.6
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    • pp.694-698
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    • 2006
  • Several marine bacterial strains were isolated from Undaria pinnatifida (Miyok in Korean). Sixty-six strains were isolated on R2A agar media at $10^{\circ}C$ and identified by a phylogenetic analysis of the 16S rRNA gene sequences. They were grouped into 10 different sequence types based on the initial sequence analysis of the 5' domain of the gene (approximately 500 bp). Full sequences of 16S rRNA gene, were obtained from one strain in each sequence type and the species-affiliation was determined using phylogenetic and sequence similarity analyses. The results of the analyses indicated that they were closely related to Psychrobacter aquimaris, P. celer, P. nivimaris, P. pulmonis, Psychromonas arctica or Bacillus psychrodurans. These bacteria are marine or psychrotrophic bacteria. Because the sporophytes of U. pinnatifida are cultured on the costal area during winter, the U. pinnatifida-associated bacteria appeared to grow at low temperatures. U. pinnatifida sporophytes can be a good source for the isolation of psychrotrophic bacteria.

Isolation and Bacteriological Characteristics of Spiral form Bacteria from Patient with Epidemic Pulmonary Hemorrhagic Fever (유행성 출혈형 폐염양 질환의 병원균 분리와 세균학적 특성)

  • 이봉기;유주현;이원영;김주덕
    • Korean Journal of Microbiology
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    • v.23 no.3
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    • pp.223-229
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    • 1985
  • We have previously isolated seven strains of spiral form bacteria, which have shown several similar characteristics to Leptospira from patient with epidemic pulmonary hemorrhagic fever and matural paddy water. Further studies have performed to characterize them. All of the isolated bacteria were aerobic, gram negative and spiral form and their growth were completely inhibited at temperature below $13^{\circ}C\;and\;50^{\circ}C$. In sensitivity test to drugs, the bacteria were resistant to the bile salts, 5-Fu and Amphotericine B, but sensitive to the 8-Azaguanine. The isolated bacteria as well as 5 serotypes of Leptospira interrogans reacted the patient sera and it was also shown that antisera from rabbit immunized with the isolated bacteria reacted with 5 serotypes of Leptospira interrogans. These results suggest that the isolated bacteria may belong to the genus Leptospira.

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A study on isolated microorganisms from dental cast (석고 모형으로부터 분리 배양된 미생물에 관한 연구)

  • Choi, Ju-Young
    • Journal of Technologic Dentistry
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    • v.35 no.4
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    • pp.321-332
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    • 2013
  • Purpose: This study was examined the characteristics of bacteria isolated from the dental stone that is made ??in the dental laboratory. Methods: 104 dental stones samples were collected from the 4 dental laboratory. Characteristics of bacteria were investigated by microorganism isolation culture method using a Blood Tryptic Soy Agar(TSA) medium. Results: The detected various bacteria was confirmed as pathogenic bacteria, non-pathogenic bacteria and natural bacteria. The isolated bacterial number was confirmed $2.9{\times}10^3CFU$ and maxium bacterial number of $3.0{\times}10^4CFU$. Conclusion: Therefore, infection prevention education is required, it must be to live up the hand-washing and wear protective clothing to protect themselves when working in a dental laboratory.

Isolation of Fungal Deteriogens Inducing Aesthetical Problems and Antifungal Calcite Forming Bacteria from the Tunnel and Their Characteristics (터널에서 미학적 문제를 야기하는 진균 및 항진균 활성을 가진 탄산칼슘 형성세균의 분리와 특성)

  • Park, Jong-Myong;Park, Sung-Jin;Ghim, Sa-Youl
    • Microbiology and Biotechnology Letters
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    • v.39 no.3
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    • pp.287-293
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    • 2011
  • The purpose of this study was to isolate and characterize fungal deteriogens, which induce discoloration of the cement tunnel, and calcite forming bacteria (CFBs), which have antifungal activity against fungal deteriogens. Isolation of mold, bacteria and yeast was performed using several solid media and partially identified using internal transcribed spacer (ITS); 5.8S rRNA gene sequencing and 16s rDNA sequencing. A total of 19 microbial strains were identified with the most widely distributed fungal strain being Cladospirum sphaerospermum. In addition, five bacteria derived from the tunnel were identified as CFBs. Amongst the latter, Bacillus aryabhatti KNUC205 exhibited antifungal activity against Cladospirum sphaerospermum KNUC253 and Aspergillus niger KCTC6906 as concentrated filtered supernatants.