• 상하수도학회지
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• 제36권2호
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• pp.97-106
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• 2022

Recently microplastic (MP) biofilm is being attracted as an important environmental issue because it can act as a pollutant carrier in aqueous system. Therefore, this study investigated the MP biofilm communities originated from freshwater. The results showed the bacterial community structure of MP biofilm was distinctively different from the freshwater regardless of biofilm-forming condition and MP type. For MP biofilm communities exposed to raw freshwater, Solimonas variicoloris-like microbe, Frigidibacter albus-like microbe, Nitrospirillum amazonense-like microbe, and Pseudochroococcus couteii-like microbe became abundant, while Acinetobacter johnsonii, Macellibacteroides fermentans, and Sedimentibacter acidaminivorans-like microbe were found as major bacteria for MP biofilm communities exposed to organic rich condition. The results of this study suggest that the unique freshwater biofilm community could be formed on the MP surface.

• Fisheries and Aquatic Sciences
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• 제24권9호
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• pp.297-310
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• 2021

The rockworm gut flora was identified at each growth stage to elucidate the composition of the bacterial community. The source material was Marphysa sanguinea fed regular feed and fed biofloc at the adult stage in parallel. The systematic bacterial community composition was determined based on the next-generation sequencing method, and alpha diversity and beta diversity were conducted to access the species diversity within and between the bacterial communities, respectively. The composition of the gut flora changed considerably as the rockworms developed. The shift in the gut flora was confirmed at the phylum, family, and genera level of the bacterial communities. The Vibrio species associated with high rockworm mortality occupied 7.7% of the gut flora at the larval stage; however, they disappeared in the healthy adult gut. Moreover, different gut flora was observed between adults fed regular feed and those fed biofloc. Specifically in the biofloc-fed adult gut, several immune relevant and water-purifying bacteria were detected. The biofloc-fed adult gut flora could decompose and mineralize organic sediment, and thus be effectively utilized for integrated multitrophic aquaculture. The Venn diagram revealed that only two bacterial species were shared throughout all growth stages, and the biofloc-fed adults exhibited the highest diversity within the bacterial community.

• Fisheries and Aquatic Sciences
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• 제26권10호
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• pp.593-604
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• 2023

Post-harvest handling and hygienic level of aquatic products significantly affect the quality and level of safety. Cold chain control is one of the determining factors for the quality of fish and the bacterial community that grows on the fish. Identification of spoilage bacteria and pathogens in aquatic products must be made because it will determine the physical and chemical quality. A molecular identification method with high sensitivity is the solution. This study aims to identify the quality of fish and bacterial communities that grow. The research procedures included sample collection, pH measurement, drip loss measurement, transportation and cold storage treatment, DNA extraction, DNA sequencing, sequence analysis, and bioinformatics analysis. The conclusion obtained from this study is that the simulation of the cold chain system applied to eastern little tuna does not significantly affect changes in the water activity value, pH, and drip loss. The insignificant change indicates that the eastern little tuna samples are still in good quality. The bioinformatics analysis showed the highest diversity and abundance of the bacterial community came from the Gammaproteobacterial class.

• Asian-Australasian Journal of Animal Sciences
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• 제28권10호
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• pp.1433-1441
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• 2015

This study examined changes of rumen fermentation, ruminal bacteria biodiversity and abundance caused by nitrate addition with Ion Torrent sequencing and real-time polymerase chain reaction. Three rumen-fistulated steers were fed diets supplemented with 0%, 1%, and 2% nitrate (dry matter %) in succession. Nitrate supplementation linearly increased total volatile fatty acids and acetate concentration obviously (p = 0.02; p = 0.02; p<0.01), butyrate and isovalerate concentration numerically (p = 0.07). The alpha (p>0.05) and beta biodiversityof ruminal bacteria were not affected by nitrate. Nitrate increased typical efficient cellulolytic bacteria species (Ruminococcus flavefaciens, Ruminococcus ablus, and Fibrobacter succinogenes) (p<0.01; p = 0.06; p = 0.02). Ruminobactr, Sphaerochaeta, CF231, and BF311 genus were increased by 1% nitrate. Campylobacter fetus, Selenomonas ruminantium, and Mannheimia succiniciproducens were core nitrate reducing bacteria in steers and their abundance increased linearly along with nitrate addition level (p<0.01; p = 0.02; p = 0.04). Potential nitrate reducers in the rumen, Campylobacter genus and Cyanobacteria phyla were significantly increased by nitrate (p<0.01; p = 0.01).To the best of our knowledge, this was the first detailed view of changes in ruminal microbiota by nitrate. This finding would provide useful information on nitrate utilization and nitrate reducer exploration in the rumen.

• Asian-Australasian Journal of Animal Sciences
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• 제21권4호
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• pp.603-615
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• 2008

Dietary fiber is an inevitable component in pig diets. In non-ruminants, it may influence many physiological processes in the gastrointestinal tract (GIT) such as transit time as well as nutrient digestion and absorption. Moreover, dietary fiber is also the main substrate of intestinal bacteria. The bacterial community structure is largely susceptible to changes in the fiber content of a pig's diet. Indeed, bacterial composition in the lower GIT will adapt to the supply of high levels of dietary fiber by increased growth of bacteria with cellulolytic, pectinolytic and hemicellulolytic activities such as Ruminococcus spp., Bacteroides spp. and Clostridium spp. Furthermore, there is growing evidence for growth promotion of beneficial bacteria, such as lactobacilli and bifidobacteria, by certain types of dietary fiber in the small intestine of pigs. Studies in rats have shown that both phosphorus (P) and calcium (Ca) play an important role in the fermentative activity and growth of the intestinal microbiota. This can be attributed to the significance of P for the bacterial cell metabolism and to the buffering functions of Ca-phosphate in intestinal digesta. Moreover, under P deficient conditions, ruminal NDF degradation as well as VFA and bacterial ATP production are reduced. Similar studies in pigs are scarce but there is some evidence that dietary fiber may influence the ileal and fecal P digestibility as well as P disappearance in the large intestine, probably due to microbial P requirement for fermentation. On the other hand, fermentation of dietary fiber may improve the availability of minerals such as P and Ca which can be subsequently absorbed and/or utilized by the microbiota of the pig's large intestine.

• 한국토양비료학회지
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• 제47권6호
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• pp.496-499
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• 2014

The present study investigated variations in soil microbial communities and the chemical properties of forest soils by differing amounts of penetrating sunlight. The soil temperature was significantly higher in higher light-penetrated soils. Higher light-penetrated soils (LP70) showed significantly more fungal communities than the lower light-penetrated soils (LP40 and LP50) (p < 0.05). The $NH_4$-N concentration in LP70 was significantly lower than those of LP40 and LP50, whereas the other chemical properties showed no significant difference among the soils. The cy19:0 to $18:1{\omega}7c$ ratio was significantly lower in LP70 than in LP 40 and LP50 showing the negative correlation of light level with microbial stresses (p < 0.05). The soil microbial communities and the chemical properties that showed positive eigenvector coefficients for PC1 were the fungi to bacteria, fungi, arbuscular mycorrhizal fungi, and Gram-positive bacteria, whereas negative eigenvector coefficients were found for $NH_4$-N, actinomycetes, Gram-negative bacteria, and bacteria. Consequently, the amount of penetrating light was responsible for microbial compositions in the forest soils in correlation with the concentration of $NH_4$-N and soil temperature.

• 미생물학회지
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• 제44권1호
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• pp.29-36
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• 2008

질소는 하수처리과정에서 제거되어야 하는 주요 오염물질 중의 하나이며, 세균 군집을 이용한 고도처리 시스템에서 생물학적 질소제거는 중요한 기술이다. 질산화반응은 생물학적 질소제거 시스템의 첫 단계로 미생물에 의해 진행된다. 암모니아는 암모니아산화세균에 의해 아질산염으로 산화되며, 그 후에 아질산염은 아질산염 산화세균에 의해 질산염으로 산화된다. 실험실 규모의 생물학적 질소제거 시스템인 변형된 eBAF 시스템, Nutrient removal laboratory 시스템과 반추기법을 적용한 rSBR 시스템의 질산화반응조 시료에서 16S rRNA 유전자를 이용한 terminal restriction fragment length polymorphism (T-RFLP) 방법으로 아질산염 산화세균군집을 분석하였다. 제한효소로 형성된 단편의 클러스터분석에서 Nitrobacter 군집은 각각의 폐수처리 시스템에 따라 군집의 차이가 있음이 나타났다. 그러나 Nitrospira 군집의 클러스터분석에서는 액체와 담체의 서식지 환경 차이에 의해 군집이 구분되었다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2000년도 Proceedings of 2000 KSAM International Symposium and Spring Meeting
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• pp.150-156
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• 2000

Oligotrophic bacteria isolated from forest soil showed a specific community consisting of various taxonomic groups compared with those in other soil or aquatic habitats. Based on the cell shape, the isolates were divided into four groups: regular rod, curved/spiral rod, irregular rod, and prosthecate bacteria. The cellular fatty acids 60 oligotrophic isolates were analyzed. The 30 fatty acids which were identified or characterized are classified. At the dendrogram based on cellular fatty acid composition, four clusters(I-IV) were separated at a euclidian distance of about 50. Cluster 3 and 4-a strains were containing Q-8, these strains are accommodated in the Proteobacteria gamma and beta subdivision. The chemotaxonomic profiles of the cluster 4-a strains showed good agreement with those of the genus Burkholderia. Cluster 3 was characterized by the presence of branched-chain fatty acids, iso-C15:0, iso-C17:1, and iso-C17:0 as the major components. These chemotaxonomy suggested the close relationship of the isolates with Xathomonas/Sterotrophomonas group. Based on the 16S rDNA sequence analysis, the two representative strains(MH256 and MA828) of cluster 3 showed the close relation to genera, Xathomonas/Sterotrophomonas, but were not included in these genera. These strains were even further away from core Xanthomonas, and clearly were seen to branch outside the cluster formed by the Sterotrophomonas maltophilia. MH256 and MA828 16S rDNA sequence was different enough to put new genus on a separate branch. The isolates with Q-10 were also studied. They are corresponded to the two large groups in Proteobacteria alpha subdivision. One was incorporated in the genus Bradyrhizobium cluster, which also includes Agromonas, a genus for oligotrophic bacteria. The strains of the other group showed high similarity to the genus Agrobacterium.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.394-401
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• 2016

Recently, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) system, a genome editing technology, was shown to be versatile in treating several antibiotic-resistant bacteria. In the present study, we applied the CRISPR/Cas9 technology to kill extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli. ESBL bacteria are mostly multidrug resistant (MDR), and have plasmid-mediated antibiotic resistance genes that can be easily transferred to other members of the bacterial community by horizontal gene transfer. To restore sensitivity to antibiotics in these bacteria, we searched for a CRISPR/Cas9 target sequence that was conserved among >1,000 ESBL mutants. There was only one target sequence for each TEM- and SHV-type ESBL, with each of these sequences found in ~200 ESBL strains of each type. Furthermore, we showed that these target sequences can be exploited to re-sensitize MDR cells in which resistance is mediated by genes that are not the target of the CRISPR/Cas9 system, but by genes that are present on the same plasmid as target genes. We believe our Re-Sensitization to Antibiotics from Resistance (ReSAFR) technology, which enhances the practical value of the CRISPR/Cas9 system, will be an effective method of treatment against plasmid-carrying MDR bacteria.

• 한국환경보건학회지
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• 제30권2호
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• pp.98-103
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• 2004

To evaluate the quality of drinking water and contamination sources in a rural community, this study was carried out on the summer of 2003 at Shin-Dong Myun, Chun-Cheon, Gang-Won province. Seventy three drinking water samples were collected from three different types of water supply systems. Sources of contamination were identified and the public perception of water quality area were evaluated. The findings of this study are as follows; Drinking water was mainly obtained from Local Water and Simple Piped Water Supply Systems, and pollution sources varied over the study area. Most of residents (＞63%) were satisfied with the quality of drinking water and could not identify any contamination sources. Corresponding to this perception, measured water quality parameters generally met the Drinking Water Quality Standards (＞64%). However, approximately 35.6% of samples exceeded the regulation for Nㅒ$_3$$^{-}$$_{-}$N, coliform, and general bacteria. The water quality was significantly different among the three water supply systems, and between the potentially contaminated areas and the rest of the areas (p＜0.05). In the potentially contaminated areas, the levels of coliform and general bacteria contamination were significantly higher than the rest of the areas (p＜0.05). The coliform and general bacteria values of Simple Piped Water were significantly higher than Own Piped Water＇s, and the NO$_3$$^{-}$$_{-}$N values of Own Piped Water were significantly higher than those of water＇s (p＜0.05). Based on this study, NO$_3$$^{-}$$_{-}$N, coliform, and general bacteria were identified as a major problem of drinking water. To regularly manage drinking water supply systems, to identify contamination sources, and to add drainage systems are required in the study area.